@article {pmid39713446,
year = {2024},
author = {Gaddy, KE and Septer, AN and Mruk, K and Milton, ME},
title = {A mutualistic model bacterium is lethal to non-symbiotic hosts via the type VI secretion system.},
journal = {bioRxiv : the preprint server for biology},
volume = {},
number = {},
pages = {},
doi = {10.1101/2024.12.13.628426},
pmid = {39713446},
issn = {2692-8205},
abstract = {What makes a bacterium pathogenic? Since the early days of germ theory, researchers have categorized bacteria as pathogens or non-pathogens, those that cause harm and those that do not, but this binary view is not always accurate. Vibrio fischeri is an exclusive mutualistic symbiont found within the light organs of Hawaiian bobtail squid. This symbiotic interaction requires V. fischeri to utilize a range of behaviors and produce molecules that are often associated with pathogenicity. This juxtaposition of employing "pathogenic" behaviors for a symbiotic relationship led the field to focus on how V. fischeri establishes a beneficial association with its host. In this study, we observe that V. fischeri induces mortality in zebrafish embryos and Artemia nauplii. Non-lethal doses of V. fischeri leads to zebrafish growth delays and phenotypes indicative of disease. Our data also provide evidence that the conserved type VI secretion system on chromosome I (T6SS1) plays a role in the V. fischeri -induced mortality of zebrafish embryos and Artemia nauplii. These results support the hypothesis that the V. fischeri T6SS1 is involved in eukaryotic cell interactions. Despite its traditional view as a beneficial symbiont, we provide evidence that V. fischeri is capable of harming aquatic organisms, indicating its potential to be pathogenic toward non-symbiotic hosts.},
}

@article {pmid39713146,
year = {2024},
author = {He, S and Gao, L and Zhang, Z and Ming, Z and Gao, F and Ma, S and Zou, M},
title = {Diversity analysis of microorganisms on the surface of four summer fruit varieties in Baotou, Inner Mongolia, China.},
journal = {PeerJ},
volume = {12},
number = {},
pages = {e18752},
pmid = {39713146},
issn = {2167-8359},
mesh = {*Fruit/microbiology ; China ; *RNA, Ribosomal, 16S/genetics ; Microbiota/genetics ; Malus/microbiology ; Prunus domestica/microbiology ; Bacteria/genetics/classification/isolation & purification ; Fungi/classification/genetics/isolation & purification ; Proteobacteria/genetics/isolation & purification ; Firmicutes/genetics/isolation & purification ; High-Throughput Nucleotide Sequencing ; Bacteroidetes/genetics/isolation & purification/classification ; Biodiversity ; },
abstract = {Improper storage of post-harvest fruits leads to significant losses, especially due to microbial-induced decay. Understanding the naturally occurring microbial communities on fruit surfaces and their functions is the first step in the development of new strategies for controlling post-harvest fruit decay. These new strategies could generate significant economic value by improving fruit preservation and extending the shelf-life of fruit. In the present study, 16S rRNA and ITS high-throughput sequencing technologies were used to analyze the diversity and composition of microorganisms on the surfaces of four different fruit varieties: three plum varieties and one apple variety, all from the same orchard in Donghe District, Baotou City, China. The results displayed no notable difference in bacterial diversity on the surfaces of the four varieties of fruits (P > 0.05), but there were significant differences in fungal diversity (P < 0.05). The most abundant bacterial phyla detected on the fruit surfaces were Proteobacteria, Bacteroidota, and Firmicutes; the most abundant fungal phyla were Ascomycota, Basidiomycota, and Mortierellomycota. Though microbial compositions on the fruit surfaces differed between the fruits, the surface microbial community structure of the three plum varieties exhibited higher similarity, indicating that fruit type is a key factor influencing the composition of surface microorganisms. There were also differences in the epidermal microbial community composition between the fruits involved in this study and fruits of the same species reported from other regions, suggesting that geographical factors also play a critical role in microbial composition. The correlation analysis revealed significant associations between the microorganisms with the highest abundance on the surface of the fruits, suggesting the existence of symbiotic and mutualistic relationships between these microorganisms, but the specific mechanisms behind these relationships need to be further explored. This study provides a basis for the establishment of post-harvest fruit preservation strategies.},
}

*Fruit/microbiology
China
*RNA, Ribosomal, 16S/genetics
Microbiota/genetics
Malus/microbiology
Prunus domestica/microbiology
Bacteria/genetics/classification/isolation & purification
Fungi/classification/genetics/isolation & purification
Proteobacteria/genetics/isolation & purification
Firmicutes/genetics/isolation & purification
High-Throughput Nucleotide Sequencing
Bacteroidetes/genetics/isolation & purification/classification
Biodiversity

@article {pmid39713078,
year = {2024},
author = {Maiti, A and Mondal, S and Choudhury, S and Bandopadhyay, A and Mukherjee, S and Sikdar, N},
title = {Oncometabolites in pancreatic cancer: Strategies and its implications.},
journal = {World journal of experimental medicine},
volume = {14},
number = {4},
pages = {96005},
pmid = {39713078},
issn = {2220-315X},
abstract = {Pancreatic cancer (PanCa) is a catastrophic disease, being third lethal in both the genders around the globe. The possible reasons are extreme disease invasiveness, highly fibrotic and desmoplastic stroma, dearth of confirmatory diagnostic approaches and resistance to chemotherapeutics. This inimitable tumor microenvironment (TME) or desmoplasia with excessive extracellular matrix accumulation, create an extremely hypovascular, hypoxic and nutrient-deficient zone inside the tumor. To survive, grow and proliferate in such tough TME, pancreatic tumor and stromal cells transform their metabolism. Transformed glucose, glutamine, fat, nucleotide metabolism and inter-metabolite communication between tumor and TME in synergism, impart therapy resistance, and immunosuppression in PanCa. Thus, a finer knowledge of altered metabolism would uncover its metabolic susceptibilities. These unique metabolic targets may help to device novel diagnostic/prognostic markers and therapeutic strategies for better management of PanCa. In this review, we sum up reshaped metabolic pathways in PanCa to formulate detection and remedial strategies of this devastating disease.},
}

@article {pmid39712641,
year = {2024},
author = {Shalini, TS and Prathiviraj, R and Senthilraja, P},
title = {Metagenomic analysis and bioactive profiling of kombucha fermentation: antioxidant, antibacterial activities, and molecular docking insights into gastric cancer therapeutics.},
journal = {Toxicology research},
volume = {13},
number = {6},
pages = {tfae224},
pmid = {39712641},
issn = {2045-452X},
abstract = {Kombucha is fermented and produced with a biofilm called a symbiotic culture of bacteria and yeast, which is drunk all over the world for its beneficial effects on human health and energy levels. The metagenomic study of kombucha frequently detected microorganisms in proteobacteria, firmicutes, and actinobacteria. And also, yeast and fungi are Ascomycota and Basidiomycota is present in green leaf and sugarcane juice fermented kombucha. The kombucha extracts' biological activities were assessed using pH, total phenolic content, antioxidant, antibacterial, and anticancer activity. Fermentation may enhance biological activity and the generation of bioactive substances. These results showed the pH -3.1 ± 0.2 and TPC -0.721 μg/mL of gallic acid equivalent. The antioxidant radicals scavenging activity of kombucha was evaluated by DPPH, ABTS, H2O2 and TAC. The bioactive chemicals identified by FT-IR and HR-LC/MS analysis of Kombucha totaled 45 components. The identified compounds were further move on to perform molecular docking study against gastric cancer target proteins 4H9M, 2DQ7 and 1TVO are binding with Nequinate compounds showing best LibDock scores 105.12, 114.49, and 108.97. So, this study suggests that knowledge can potentially active bioactive compounds are present in kombucha and it's stimulated the mechanism of gastrointestinal transit. Additionally, the metagenomic analysis gives strength to understand the bacterial and fungal distribution and its molecular mechanism from Kombucha.},
}

@article {pmid39710945,
year = {2025},
author = {Keller, V and Calchera, A and Otte, J and Schmitt, I},
title = {Genomic features of lichen-associated black fungi.},
journal = {IUBMB life},
volume = {77},
number = {1},
pages = {e2934},
pmid = {39710945},
issn = {1521-6551},
mesh = {*Lichens/genetics/microbiology ; *Genome, Fungal ; *Phylogeny ; *Ascomycota/genetics ; Symbiosis/genetics ; Genomics ; },
abstract = {Lichens are mutualistic associations consisting of a primary fungal host, and one to few primary phototrophic symbiont(s), usually a green alga and/or a cyanobacterium. They form complex thallus structures, which provide unique and stable habitats for many other microorganisms. Frequently isolated from lichens are the so-called black fungi, or black yeasts, which are mainly characterized by melanized cell walls and extremophilic lifestyles. It is presently unclear in which ways these fungi interact with other members of the lichen symbiosis. Genomic resources of lichen-associated black fungi are needed to better understand the physiological potential of these fungi and shed light on the complexity of the lichen consortium. Here, we present high-quality genomes of 14 black fungal lineages, isolated from lichens of the rock-dwelling genus Umbilicaria. Nine of the lineages belong to the Eurotiomycetes (Chaetothyriales), four to the Dothideomycetes, and one to the Arthoniomycetes, representing the first genome of a black fungus in this class. The PacBio-based assemblies are highly contiguous (5-42 contigs per genome, mean coverage of 79-502, N50 of 1.0-7.3 mega-base-pair (Mb), Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness generally ≥95.4%). Most contigs are flanked by a telomere sequence, suggesting we achieved near chromosome-level assemblies. Genome sizes range between 26 and 44 Mb. Transcriptome-based annotations yielded ~11,000-18,000 genes per genome. We analyzed genome content with respect to repetitive elements, biosynthetic genes, and effector genes. Each genome contained a polyketide synthase gene related to the dihydroxynaphthalene-melanin pathway. This research provides insights into genome content and metabolic potential of these relatively unknown, but frequently encountered lichen associates.},
}

*Lichens/genetics/microbiology
*Genome, Fungal
*Phylogeny
*Ascomycota/genetics
Symbiosis/genetics
Genomics

@article {pmid39710701,
year = {2024},
author = {Ardpairin, J and Subkrasae, C and Dumidae, A and Pansri, S and Homkaew, C and Meesil, W and Kumchantuek, T and Phoungpetchara, I and Dillman, AR and Pavesi, C and Bode, HB and Tandhavanant, S and Thanwisai, A and Vitta, A},
title = {Symbiotic bacteria associated with entomopathogenic nematodes showed molluscicidal activity against Biomphalaria glabrata, an intermediate host of Schistosoma mansoni.},
journal = {Parasites & vectors},
volume = {17},
number = {1},
pages = {529},
pmid = {39710701},
issn = {1756-3305},
support = {PHD / 0086/2561//the National Research Council of Thailand/ ; R2567B034//Naresuan University (NU) and the National Science, Research and Innovation Fund (NSRF)/ ; },
mesh = {Animals ; *Biomphalaria/microbiology/parasitology ; *Molluscacides/pharmacology ; *Symbiosis ; Photorhabdus/genetics/physiology ; Xenorhabdus/genetics/physiology ; Schistosoma mansoni/physiology/drug effects ; },
abstract = {BACKGROUND: Biomphalaria glabrata acts as the intermediate host of schistosomes that causes human schistosomiasis. Symbiotic bacteria, Xenorhabdus and Photorhabdus associated with Steinernema and Heterorhabditis, produce secondary metabolites with several biological activities. Controlling B. glabrata is a potential strategy to limit the transmission of schistosomiasis. The aims of this study were to identify Xenorhabdus and Photorhabdus bacteria based on recA sequencing and evaluate their molluscicidal activity against B. glabrata snail.

RESULTS: A total of 31 bacterial isolates belonging to Xenorhabdus (n = 19) and Photorhabdus (n = 12) (X. ehlersii, X. stockiae, X. indica, X. griffinae, P. luminescens, P. akhurstii, and P. laumondii subsp. laumondii were molecularly identified based on recA sequencing. Five isolates of bacterial extracts showed potential molluscicide, with 100% snail mortality. P. laumondii subsp. laumondii (bALN19.5_TH) showed the highest effectiveness with lethal concentration (LC) values of 54.52 µg/mL and 89.58 µg/mL for LC50 and LC90, respectively. Histopathological changes of the snail were observed in the head-foot region, which showed ruptures of the epithelium covering the foot and deformation of the muscle fiber. A hemocyte of the treated snails was observed in the digestive tubules of the digestive glands. The hermaphrodite glands of treated snails showed a reduction in the number of spermatozoa, degeneration of oocytes, and deformation and destruction in the hermaphrodite gland. In addition, liquid chromatography-tandem mass spectrometry (LC-MS/MS) of three symbiotic bacteria contained compounds such as GameXPeptide, Xenofuranone, and Rhabdopeptide.

CONCLUSIONS: Five bacterial extracts showed good activity against B. glabrata, especially P. laumondii subsp. laumondii and X. stockiae, which produced virulent secondary metabolites resulting in the death of the snails. They also caused histopathological alterations in the foot, digestive glands, and hermaphrodite glands of the snails. This study suggests that extracts from these bacteria show promise as molluscicides for the control of B. glabrata.},
}

Animals
*Biomphalaria/microbiology/parasitology
*Molluscacides/pharmacology
*Symbiosis
Photorhabdus/genetics/physiology
Xenorhabdus/genetics/physiology
Schistosoma mansoni/physiology/drug effects

@article {pmid39710632,
year = {2024},
author = {Krueger, CB and Costa Netto, JR and Arifuzzaman, M and Fritschi, FB},
title = {Characterization of genetic diversity and identification of genetic loci associated with carbon allocation in N2 fixing soybean.},
journal = {BMC genomics},
volume = {25},
number = {1},
pages = {1233},
pmid = {39710632},
issn = {1471-2164},
support = {2120-172-0142 and 2220-172-0149//United Soybean Board/ ; 2120-172-0142 and 2220-172-0149//United Soybean Board/ ; 2120-172-0142 and 2220-172-0149//United Soybean Board/ ; 2120-172-0142 and 2220-172-0149//United Soybean Board/ ; },
mesh = {*Glycine max/genetics/metabolism/growth & development ; *Quantitative Trait Loci ; *Genetic Variation ; *Nitrogen Fixation/genetics ; *Carbon/metabolism ; Phenotype ; Genotype ; Polymorphism, Single Nucleotide ; Plant Roots/genetics/metabolism/growth & development ; Biomass ; Chromosome Mapping ; },
abstract = {BACKGROUND: Efficient capture and use of resources is critical for optimal plant growth and productivity. Both shoot and root growth are essential for resource acquisition, namely light and CO2 by the shoot and water and mineral nutrients by roots. Soybean [Glycine max (L.) Merr.], one of the most valuable crops world-wide, uses an additional strategy, symbiotic N fixation (SNF), for N acquisition. SNF relies on development of specialized root organs known as nodules, which represent a distinct C sink. The genetic diversity of C partitioning in N fixing soybean to shoots, roots, and nodules has not been previously investigated but is valuable to better understand consequences of differential C allocation and to develop genetic resources, including identification of quantitative trait loci (QTLs).

RESULTS: A diversity panel of 402 soybean genotypes was phenotyped outdoors in a deep-tube system without addition of mineral N to measure allocation of biomass to the shoot, root, and nodules, as well as to determine nodule number, mean nodule biomass, and total shoot N accumulation. Wide ranges in phenotypes were observed for each of these traits, demonstrating extensive natural diversity in C partitioning and SNF in soybean. Using a set of 35,647 single nucleotide polymorphism (SNP) markers, we identified 121 SNPs tagging 103 QTLs that include both 84 novel and 19 previously identified QTLs for the eight examined traits. A candidate gene search identified 79 promising gene models in the vicinity of these QTLs. Favorable alleles of QTLs identified here may be used in breeding programs to develop elite cultivars with altered C partitioning.

CONCLUSIONS: This study provides novel insights into the diversity of biomass allocation in soybean and illustrates that the traits measured here are heritable and quantitative. QTLs identified in this study can be used in genomic prediction models as well as for further investigation of candidate genes and their roles in determining partitioning of fixed C. Enhancing our understanding of C partitioning in plants may lead to elite cultivars with optimized resource use efficiencies.},
}

*Glycine max/genetics/metabolism/growth & development
*Quantitative Trait Loci
*Genetic Variation
*Nitrogen Fixation/genetics
*Carbon/metabolism
Phenotype
Genotype
Polymorphism, Single Nucleotide
Plant Roots/genetics/metabolism/growth & development
Biomass
Chromosome Mapping

@article {pmid39709880,
year = {2024},
author = {Jiang, S and Lu, H and Xie, Y and Zhou, T and Dai, Z and Sun, R and He, L and Li, C},
title = {Toxicity of microplastics and nano-plastics to coral-symbiotic alga (Dinophyceae Symbiodinium): Evidence from alga physiology, ultrastructure, OJIP kinetics and multi-omics.},
journal = {Water research},
volume = {273},
number = {},
pages = {123002},
doi = {10.1016/j.watres.2024.123002},
pmid = {39709880},
issn = {1879-2448},
abstract = {Corals are representative of typical symbiotic organisms. The coral-algal (Symbiodinium spp.) symbiosis drives the productivity of entire coral reefs. In recent years, microplastics (MPs) and nano-plastics (NPs) have been shown to disrupt this symbiosis, leading to coral bleaching. However, how MPs/NPs affect the Symbiodinium spp. is less thoroughly explored. In this work, Dinophyceae Symbiodinium was employed as a model to study the toxicity effects of MPs and NPs with different concentrations (covering environment-related concentration) toward algae in terms of cellular responses, ultrastructure, OJIP kinetics curve and multi-omics. MPs and NPs caused adverse effects on algae growth throughout whole growing phase, with only slight differences observed in the maximal inhibition ratio. In addition to cell surface shrinkage, holes and plate sutures shedding of algae, the presence of distorted thylakoids, plasmolysis and expanded vesicle volume were observed due to the oxidative stress and physical damage caused by MPs/NPs. The results of OJIP kinetics and JIP-test revealed that MPs/NPs-induced deactivation of oxygen-releasing complex (OEC) centers, reduced electron transfer (photosystem II, PSII), and inefficient energy conversion of antenna proteins were the primary factors for photosynthesis reduction. Weighted correlation network analysis (WGCNA) showed that the impairment of photosynthesis further induces metabolic disturbances, including reactive oxygen species (ROS) generation and nucleotide metabolism dysregulation, thereby exacerbating DNA damage in the algae. Proteomics further validate the accuracy of our results and underscore the significance of the phosphatidylinositol (PI) signaling system in algae responding to MP/NPs acclimation. Collectively, our findings provide comprehensive insights into the ecotoxicity of NPs/MPs on symbiotic algae.},
}

@article {pmid39709662,
year = {2024},
author = {Cao, L and Wang, L and Qi, Y and Yang, S and Gao, J and Liu, Q and Song, L and Hu, R and Wang, Z and Zhang, H},
title = {Enhanced effect of ferrous sulfate on nitrogen retention and PBAT degradation during co-composting by combing with biochar-loaded FN1 bacterial composites.},
journal = {Journal of environmental management},
volume = {373},
number = {},
pages = {123749},
doi = {10.1016/j.jenvman.2024.123749},
pmid = {39709662},
issn = {1095-8630},
abstract = {The treatment of biodegradable plastics through composting has garnered increasing attention. This study aimed to investigate the effects of Biochar FN1 bacteria and ferrous sulfate on nitrogen retention, greenhouse gas emissions, and degradable plastics during composting and to elucidate their synergistic mechanisms on microbial communities. Compared with the control, applying biochar-loaded FN1 bacteria composites combined with Ferrous sulfate (SGC) markedly accelerated organic matter degradation and reduced cumulative CO2 and NH3 emissions. The synergistic interaction between the composites and Ferrous sulfate significantly enhanced NH4[+]-N levels in the thermophilic phase and NO3[-]-N levels in the cooling phase, ultimately decreasing nitrogen loss by 14.9% (P < 0.05) and increasing the seed germination index (GI) by 22.5% (P < 0.05). Additionally, PBAT plastic degradation was improved by 31.6% (P < 0.05). The SGC treatment also altered the richness and diversity of the bacterial community in both the compost and the PBAT plastic sphere, particularly affecting Sphingobacterium, Pseudomonas, and Flavobacterium at the genus level. Symbiotic network analysis and Redundancy Analysis revealed that these functional degradation bacteria were significantly positively correlated with NO3[-]-N levels and PBAT degradation. Furthermore, structural equation modelling indicated a positive relationship between PBAT degradation rate and composting temperature (r = 0.69, p < 0.05). The findings suggested that Fe[2+] not only enhanced the FN1 activity but also promoted PBAT degradation by increasing ·OH content on the PBAT plastic sphere. Overall, the combined use of biochar-loaded FN1 bacteria and Ferrous sulfate effectively supports nitrogen retention and plastic degradation during composting.},
}

@article {pmid39709489,
year = {2024},
author = {Chen, S and Qi, H and Zhu, X and Liu, T and Fan, Y and Su, Q and Gong, Q and Jia, C and Liu, T},
title = {Screening and identification of antimicrobial peptides from the gut microbiome of cockroach Blattella germanica.},
journal = {Microbiome},
volume = {12},
number = {1},
pages = {272},
pmid = {39709489},
issn = {2049-2618},
mesh = {Animals ; *Gastrointestinal Microbiome/drug effects ; *Antimicrobial Peptides/pharmacology ; Mice ; *Anti-Bacterial Agents/pharmacology ; Microbial Sensitivity Tests ; Bacteria/drug effects/classification/isolation & purification ; Humans ; Blattellidae/microbiology ; Cockroaches/microbiology ; },
abstract = {BACKGROUND: The overuse of antibiotics has led to lethal multi-antibiotic-resistant microorganisms around the globe, with restricted availability of novel antibiotics. Compared to conventional antibiotics, evolutionarily originated antimicrobial peptides (AMPs) are promising alternatives to address these issues. The gut microbiome of Blattella germanica represents a previously untapped resource of naturally evolving AMPs for developing antimicrobial agents.

RESULTS: Using the in-house designed tool "AMPidentifier," AMP candidates were mined from the gut microbiome of B. germanica, and their activities were validated both in vitro and in vivo. Among filtered candidates, AMP1, derived from the symbiotic microorganism Blattabacterium cuenoti, demonstrated broad-spectrum antibacterial activity, low cytotoxicity towards mammalian cells, and a lack of hemolytic effects. Mechanistic studies revealed that AMP1 rapidly permeates the bacterial cell and accumulates intracellularly, resulting in a gradual and mild depolarization of the cell membrane during the initial incubation period, suggesting minimal direct impact on membrane integrity. Furthermore, observations from fluorescence microscopy and scanning electron microscopy indicated abnormalities in bacterial binary fission and compromised cell structure. These findings led to the hypothesis that AMP1 may inhibit bacterial cell wall synthesis. Furthermore, AMP1 showed potent antibacterial and wound healing effects in mice, with comparable performances of vancomycin.

CONCLUSIONS: This study exemplifies an interdisciplinary approach to screening safe and effective AMPs from natural biological tissues, and our identified AMP 1 holds promising potential for clinical application.},
}

Animals
*Gastrointestinal Microbiome/drug effects
*Antimicrobial Peptides/pharmacology
Mice
*Anti-Bacterial Agents/pharmacology
Microbial Sensitivity Tests
Bacteria/drug effects/classification/isolation & purification
Humans
Blattellidae/microbiology
Cockroaches/microbiology

@article {pmid39709001,
year = {2024},
author = {Mao, B and Wang, YY and Li, SY and Fu, Y and Xiao, YL and Wang, YF},
title = {A potential role for the interaction of Wolbachia surface proteins with the Drosophila microtubulin in maintenance of endosymbiosis and affecting spermiogenesis.},
journal = {Journal of insect physiology},
volume = {},
number = {},
pages = {104743},
doi = {10.1016/j.jinsphys.2024.104743},
pmid = {39709001},
issn = {1879-1611},
abstract = {Wolbachia, as a widely infected intracellular symbiotic bacterium in Arthropoda, is able to manipulate the reproduction of insect hosts for facilitating their own transmission. Cytoplasmic incompatibility (CI) is the most common phenotype that Wolbachia induced in insect hosts where they resulted in the failure of uninfected egg hatch when fertilized with the sperm derived from Wolbachia-infected males, suggesting that the sperm are modified by Wolbachia during spermatogenesis. Although the molecular mechanisms of CI are beginning to be understood, the effects of Wolbachia on the symbiotic relationship and the proper dynamics of spermatogenesis have not yet been fully investigated. We report here that Wolbachia infection induced a significant upregulation of betaTub85D in the testis of Drosophila melanogaster. Knockdown of betaTub85D in fly testes resulted in significant decrease in the copy number of Wolbachia surface protein gene (wsp), indicating a notable reduction of Wolbachia density. Pull-down analyses revealed that WSP interacted with the betaTub85D of D. melanogaster. Wolbachia infection altered the interactome between betaTub85D and other proteins in the testes, and may thus change the protein synthesis and metabolic pathways. Wolbachia infection induced not only an interaction of betaTub85D with Mst77F but also increase in phosphorylated Mst77F. These results suggest that Wolbachia WSP protein might play important roles in anchoring the endosymbiont to the host's cytoskeleton and consequently interfere the interactions among key proteins involved in spermatogenesis in the insect host testes, resulting in modified sperm.},
}

@article {pmid39708146,
year = {2024},
author = {Zhao, L and Zhang, S and Li, J and Zhang, C and Xiao, R and Bai, X and Xu, H and Zhang, F},
title = {Unveiling Diversity and Function: Venom-Associated Microbes in Two Spiders, Heteropoda venatoria and Chilobrachys guangxiensis.},
journal = {Microbial ecology},
volume = {87},
number = {1},
pages = {156},
pmid = {39708146},
issn = {1432-184X},
mesh = {Animals ; *Spiders/microbiology/physiology ; *Spider Venoms ; *Bacteria/genetics/classification/drug effects/isolation & purification ; Apoptosis ; Symbiosis ; Humans ; Cell Line, Tumor ; High-Throughput Nucleotide Sequencing ; Anti-Inflammatory Agents/pharmacology ; },
abstract = {Spiders are natural predators of agricultural pests, primarily due to the potent venom in their venom glands. Spider venom is compositionally complex and holds research value. This study analyzes the diversity of symbiotic bacteria in spider venom glands and venom, as well as the biological activity of culturable symbiotic bacteria. Focusing on the venom glands and venom of Heteropoda venatoria and Chilobrachys guangxiensis, we identified a diverse array of microorganisms. High-throughput sequencing detected 2151 amplicon sequence variants (ASVs), spanning 31 phyla, 75 classes, and 617 genera. A total of 125 strains of cultivable bacteria were isolated. Using the Oxford cup method, crude extracts from 46 of these strains exhibited inhibitory effects against at least one indicator bacterium. MTT (Thiazolyl blue) assays revealed that the crude extracts from 43 strains had inhibitory effects on tumor cell line MGC-803 growth. Additionally, DAPI (4',6-diamidino-2'-phenylindole) staining and flow cytometry were employed to detect cell apoptosis. The anti-inflammatory activity of nine bacterial strains was assessed using a NO assay kit and enzyme-linked immunosorbent assay (ELISA). This study further investigated the biological activity of venom, exploring the relationship between the venom and the functional activity of venom-associated bacteria.},
}

Animals
*Spiders/microbiology/physiology
*Spider Venoms
*Bacteria/genetics/classification/drug effects/isolation & purification
Apoptosis
Symbiosis
Humans
Cell Line, Tumor
High-Throughput Nucleotide Sequencing
Anti-Inflammatory Agents/pharmacology

@article {pmid39707587,
year = {2024},
author = {Corrêa, JD and Carlos, PPS and Faria, GA and Pacheco, LCR and da Costa, VS and Mendes, IRR and de Oliveira, AB and Colombo, APV},
title = {The Healthy Oral Microbiome: A Changing Ecosystem throughout the Human Lifespan.},
journal = {Journal of dental research},
volume = {},
number = {},
pages = {220345241297583},
doi = {10.1177/00220345241297583},
pmid = {39707587},
issn = {1544-0591},
abstract = {Microorganisms have co-evolved with a variety of plants and animals, developing complex symbiotic relationships with their hosts and the environment. The diversity of symbionts acquired over time help their hosts to adapt, survive, and evolve more rapidly and efficiently, improving fitness across the lifespan. Understanding these synergistic relationships between humans and their endogenous microbiota may provide valuable information on human physiology and on potential mechanisms associated with the onset of diseases. This review summarizes current data on the composition and functionality of the predominant taxa of the healthy oral microbiome across different ages and habitats within the oral cavity, critically pointing out the inconsistency of methodologies for microbiological analysis and what still needs to be validated. We discuss how early acquisition and establishment of the oral microbiome are influenced by factors such as delivery type and feeding practices, and how adolescence marks a phase of significant shifts in the oral taxa due to hormonal and behavioral transitions. During adulthood, the healthy oral microbiome may acquire multistable signatures, shaped by genetic and environmental factors, while minor changes in core microorganisms are observed in the healthy aging populations. Overall, evidence shows that the oral microbiome is a complex ecosystem, continuously modulated by several factors, since its early acquisition through adulthood and into old age. Fluctuations do happen, but a resilient core community will persist over time in most humans to maintain homeostasis. Future challenges of microbiome research will rely on our ability to define multiple age-related healthy oral microbiomes across populations, so that oral dysbiosis can be detected and managed in advance. In this context, standardization of data acquisition and analysis, as well as improvements in multidisciplinary clinical diagnosis of oral health, must be pursued for a better comprehension of the balanced host-microbiome interaction.},
}

@article {pmid39707568,
year = {2024},
author = {Yang, X and Yuan, R and Yang, S and Dai, Z and Di, N and Yang, H and He, Z and Wei, M},
title = {A salt-tolerant growth-promoting phyllosphere microbial combination from mangrove plants and its mechanism for promoting salt tolerance in rice.},
journal = {Microbiome},
volume = {12},
number = {1},
pages = {270},
pmid = {39707568},
issn = {2049-2618},
mesh = {*Oryza/microbiology/growth & development ; *Salt Tolerance ; *Plant Leaves/microbiology ; Microbiota ; Rhizosphere ; Pantoea/genetics ; Soil Microbiology ; Bacillus/genetics/isolation & purification/physiology ; Bacteria/genetics/classification/isolation & purification ; Wetlands ; Rhizophoraceae/microbiology ; Quorum Sensing ; },
abstract = {BACKGROUND: Mangrove plants growing in the high salt environment of coastal intertidal zones colonize a variety of microorganisms in the phyllosphere, which have potential salt-tolerant and growth-promoting effects. However, the characteristics of microbial communities in the phyllosphere of mangrove species with and without salt glands and the differences between them remain unknown, and the exploration and the agricultural utilization of functional microbial resources from the leaves of mangrove plants are insufficient.

RESULTS: In this study, we examined six typical mangrove species to unravel the differences in the diversity and structure of phyllosphere microbial communities between mangrove species with or without salt glands. Our results showed that a combination of salt-tolerant growth-promoting strains of Pantoea stewartii A and Bacillus marisflavi Y25 (A + Y25) was constructed from the phyllosphere of mangrove plants, which demonstrated an ability to modulate osmotic substances in rice and regulate the expression of salt-resistance-associated genes. Further metagenomic analysis revealed that exogenous inoculation with A + Y25 increased the rice rhizosphere's specific microbial taxon Chloroflexi, thereby elevating microbial community quorum sensing and ultimately enhancing ionic balance and overall microbial community function to aid salt resistance in rice.

CONCLUSIONS: This study advances our understanding of the mutualistic and symbiotic relationships between mangrove species and their phyllosphere microbial communities. It offers a paradigm for exploring agricultural beneficial microbial resources from mangrove leaves and providing the potential for applying the salt-tolerant bacterial consortium to enhance crop adaptability in saline-alkaline land. Video Abstract.},
}

*Oryza/microbiology/growth & development
*Salt Tolerance
*Plant Leaves/microbiology
Microbiota
Rhizosphere
Pantoea/genetics
Soil Microbiology
Bacillus/genetics/isolation & purification/physiology
Bacteria/genetics/classification/isolation & purification
Wetlands
Rhizophoraceae/microbiology
Quorum Sensing

@article {pmid39706716,
year = {2024},
author = {Elizondo, A and Williams, R and Anderson, S and Cresswell, K},
title = {Implementing integrated care infrastructure: A longitudinal study on the interplay of policies, interorganizational arrangements and interoperability in NHS England.},
journal = {Health policy (Amsterdam, Netherlands)},
volume = {},
number = {},
pages = {105237},
doi = {10.1016/j.healthpol.2024.105237},
pmid = {39706716},
issn = {1872-6054},
abstract = {BACKGROUND: New models of care that integrate health and social care provision around the patient require a supportive infrastructure, including interorganizational arrangements and information systems. While public policies have been designed to facilitate visions of integrated care, these often neglect the implementation of effective and efficient delivery mechanisms.

METHOD: This study examines a decade of attempts to move from fragmented health and care delivery to integrated care at scale in NHS England by developing and implementing a support infrastructure. We undertook a longitudinal qualitative investigation -encompassing interviews and documentary analysis- of the implementation of interorganizational and digital interoperability infrastructures intended to support integrated care policies.

FINDINGS: Our findings underscore the long-term symbiotic relationship between institutional interorganizational frameworks and the construction of interoperability infrastructures, emphasizing how they mutually reinforce each other to support their ongoing evolution. Iterative, flexible, and experimental approaches to implementation provide opportunities to adapt to local realities while learning in the making.

CONCLUSION: This study underlines the importance of adaptable, locally-informed implementation strategies in supporting the vision of integrated care, and the need to understand such development as a long-term, ongoing process of construction and learning.},
}

@article {pmid39706307,
year = {2024},
author = {Zhang, B and Liu, J and Cai, C and Zhou, Y},
title = {Membrane photobioreactor for biogas capture and conversion - Enhanced microbial interaction in biofilm.},
journal = {Bioresource technology},
volume = {418},
number = {},
pages = {131999},
doi = {10.1016/j.biortech.2024.131999},
pmid = {39706307},
issn = {1873-2976},
abstract = {The urgency to mitigate greenhouse gas emissions has driven interest in sustainable biogas utilization. This study investigates a 1 L enclosed membrane photobioreactor (MPBR) using a microalgae-methanotroph coculture for biogas capture. Operating with a hydraulic and solid retention time of 7 days and a biogas loading rate of 2.7 L /day, the introduction of gas membrane module increased CO2-C and CH4-C uptake rates by 12 % and 50 %, respectively. Biofilm formation on the membrane surface enhanced system performance, with imaging analyses revealing methanotroph predominantly located near the membrane surface and photosynthetic microorganisms distributed throughout. Metagenomic analysis showed shifts in key metabolic pathways, including increased abundance of soluble methane monooxygenase genes and enhanced vitamin B synthesis in the biofilm. These findings highlight the spatial organization and metabolic interactions in methanotroph-microalgae coculture system, providing insights into the role of membrane-induced biofilms in improving MPBR performance for sustainable biogas utilization.},
}

@article {pmid39703707,
year = {2024},
author = {Ma, M and Xue, H and Zhu, X and Wang, L and Niu, L and Luo, J and Cui, J and Gao, X},
title = {Symbiotic microbial population composition of Apolygus lucorum under temperature and pesticide pressures.},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1485708},
pmid = {39703707},
issn = {1664-302X},
abstract = {Insect population control using pesticides faces new challenges as global temperatures change. Symbiotic bacteria of insects play a key role in insect resistance to pesticides, and these symbiotic bacteria themselves are sensitive to the effects of temperature changes. Apolygus lucorum, a sucking pest, survives in a wide range of temperatures (15°C-35°C), and is presently controlled predominantly using the pesticide imidacloprid. Here, we investigated the effects of temperature and imidacloprid on A. lucorum microbial population composition using 16S rRNA sequencing. We found that the application of imidacloprid in high-temperature environments led to an increase in the species diversity of bacteria in the body of A. lucorum. High temperatures may disrupt the symbiotic relationship between certain bacteria and A. lucorum, such as Cedecea neteri. High temperatures led to a decrease in the abundance of Cedecea neteri. Agathobaculum butyriciproducens, Advenella migardefenensis, and Akkermansia muciniphila were very sensitive to temperature and were strongly affected by temperature changes. Microorganisms that were greatly affected by the concentration of imidacloprid in the community include Aeromonas caviae and Akkermansia muciniphila. The aim of this study is to reveal the dynamics and diversity of symbiotic bacteria of A. lucorum treated with imidacloprid at a range of temperatures. These results provide insight into new strategies for pest control in a changing climate.},
}

@article {pmid39702992,
year = {2024},
author = {Gamba, AG and Oakley, CA and Ashley, IA and Grossman, AR and Weis, VM and Suggett, DJ and Davy, SK},
title = {Oxylipin Receptors and Their Role in Inter-Partner Signalling in a Model Cnidarian-Dinoflagellate Symbiosis.},
journal = {Environmental microbiology},
volume = {26},
number = {12},
pages = {e70015},
doi = {10.1111/1462-2920.70015},
pmid = {39702992},
issn = {1462-2920},
support = {19-VUW-086//Marsden Fund of the Royal Society Te Apārangi/ ; },
mesh = {Animals ; *Symbiosis ; *Sea Anemones/physiology ; *Signal Transduction ; *Dinoflagellida/physiology/metabolism ; *Oxylipins/metabolism ; },
abstract = {Oxylipin signalling is central in biology, mediating processes such as cellular homeostasis, inflammation and molecular signalling. It may also facilitate inter-partner communication in the cnidarian-dinoflagellate symbiosis, though this aspect remains understudied. In this study, four oxylipin receptors were characterised using immunohistochemistry and immunoblotting in the sea anemone Exaiptasia diaphana ('Aiptasia'): Prostaglandin E2 receptor 2 (EP2) and 4 (EP4), Transient Receptor Potential cation channel A1 (TRPA1) and Glutamate Receptor Ionotropic, Kainate 2 (GRIK2). Receptor abundance and localisation were compared between aposymbiotic anemones and symbiotic anemones hosting either native Breviolum minutum or non-native Durusdinium trenchii. All receptors were localised to the putative symbiosome of freshly isolated symbionts, suggesting a role in host-symbiont crosstalk. EP2, EP4 and TRPA1 abundance decreased in the gastrodermis of anemones hosting B. minutum, indicating potential downregulation of pathways mediated by these receptors. In contrast, GRIK2 abundance increased in anemones hosting D. trenchii in both the epidermis and gastrodermis; GRIK2 acts as a chemosensor of potential pathogens in other systems and could play a similar role here given D. trenchii's reputation as a sub-optimal partner for Aiptasia. This study contributes to the understanding of oxylipin signalling in the cnidarian-dinoflagellate symbiosis and supports further exploration of host-symbiont molecular signalling.},
}

Animals
*Symbiosis
*Sea Anemones/physiology
*Signal Transduction
*Dinoflagellida/physiology/metabolism
*Oxylipins/metabolism

@article {pmid39702246,
year = {2024},
author = {Song, X and Ju, Y and Chen, L and Zhang, W},
title = {Strategies and tools to construct stable and efficient artificial coculture systems as biosynthetic platforms for biomass conversion.},
journal = {Biotechnology for biofuels and bioproducts},
volume = {17},
number = {1},
pages = {148},
pmid = {39702246},
issn = {2731-3654},
support = {2019YFA0904600//National Key Research and Development Program of China/ ; 2019YFA0904600//National Key Research and Development Program of China/ ; 2019YFA0904600//National Key Research and Development Program of China/ ; 2019YFA0904600//National Key Research and Development Program of China/ ; },
abstract = {Inspired by the natural symbiotic relationships between diverse microbial members, researchers recently focused on modifying microbial chassis to create artificial coculture systems using synthetic biology tools. An increasing number of scientists are now exploring these systems as innovative biosynthetic platforms for biomass conversion. While significant advancements have been achieved, challenges remain in maintaining the stability and productivity of these systems. Sustaining an optimal population ratio over a long time period and balancing anabolism and catabolism during cultivation have proven difficult. Key issues, such as competitive or antagonistic relationships between microbial members, as well as metabolic imbalances and maladaptation, are critical factors affecting the stability and productivity of artificial coculture systems. In this article, we critically review current strategies and methods for improving the stability and productivity of these systems, with a focus on recent progress in biomass conversion. We also provide insights into future research directions, laying the groundwork for further development of artificial coculture biosynthetic platforms.},
}

@article {pmid39699583,
year = {2024},
author = {Galanti, D and Jung, JH and Müller, C and Bossdorf, O},
title = {Discarded sequencing reads uncover natural variation in pest resistance in Thlaspi arvense.},
journal = {eLife},
volume = {13},
number = {},
pages = {},
pmid = {39699583},
issn = {2050-084X},
support = {764965//Horizon 2020 Framework Programme/ ; 401829393//Deutsche Forschungsgemeinschaft/ ; },
mesh = {Animals ; *Aphids/genetics ; *Plant Diseases/genetics/parasitology/microbiology ; Disease Resistance/genetics ; Genome-Wide Association Study ; Genetic Variation ; Polymorphism, Single Nucleotide/genetics ; Genome, Plant/genetics ; },
abstract = {Understanding the genomic basis of natural variation in plant pest resistance is an important goal in plant science, but it usually requires large and labor-intensive phenotyping experiments. Here, we explored the possibility that non-target reads from plant DNA sequencing can serve as phenotyping proxies for addressing such questions. We used data from a whole-genome and -epigenome sequencing study of 207 natural lines of field pennycress (Thlaspi arvense) that were grown in a common environment and spontaneously colonized by aphids, mildew, and other microbes. We found that the numbers of non-target reads assigned to the pest species differed between populations, had significant SNP-based heritability, and were associated with climate of origin and baseline glucosinolate contents. Specifically, pennycress lines from cold and thermally fluctuating habitats, presumably less favorable to aphids, showed higher aphid DNA load, i.e., decreased aphid resistance. Genome-wide association analyses identified genetic variants at known defense genes but also novel genomic regions associated with variation in aphid and mildew DNA load. Moreover, we found several differentially methylated regions associated with pathogen loads, in particular differential methylation at transposons and hypomethylation in the promoter of a gene involved in stomatal closure, likely induced by pathogens. Our study provides first insights into the defense mechanisms of Thlaspi arvense, a rising crop and model species, and demonstrates that non-target whole-genome sequencing reads, usually discarded, can be leveraged to estimate intensities of plant biotic interactions. With rapidly increasing numbers of large sequencing datasets worldwide, this approach should have broad application in fundamental and applied research.},
}

Animals
*Aphids/genetics
*Plant Diseases/genetics/parasitology/microbiology
Disease Resistance/genetics
Genome-Wide Association Study
Genetic Variation
Polymorphism, Single Nucleotide/genetics
Genome, Plant/genetics

@article {pmid39699123,
year = {2024},
author = {Wang, S and Wang, T and Gao, L and Du, H and Wang, D and Ma, M and Rennenberg, H},
title = {Iron addition promotes mercury removal from soil by Robinia pseudoacacia-rhizobia symbiosis.},
journal = {Tree physiology},
volume = {},
number = {},
pages = {},
doi = {10.1093/treephys/tpae166},
pmid = {39699123},
issn = {1758-4469},
abstract = {Iron plaques on the root surface can promote or inhibit the absorption and accumulation of heavy metals by plants. However, the mechanism by which iron regulates the response of Robinia pseudoacacia to mercury (Hg) have not been elucidated which hinders its application in divalent Hg (Hg2+) removal from Hg-contaminated soil. In this study, association analyses between transcriptome and metabolome were used to investigate effects of iron on the rhizosphere microenvironment and performance of R. pseudoacacia to assess its potential for Hg2+ removal. The results showed that the addition of 10 mg kg-1 iron significantly increased the development of iron plaques on root surface, and reduced the secretion of low-molecular-weight organic acids by roots, thereby changing rhizosphere soil characteristics and decreasing total Hg in roots. In addition, the secretion of choline supported signal transduction and enhanced the interaction between R. pseudoacacia and rhizobia, thereby inducing resistance to Hg2+. Anti-oxidative enzyme activities were increased and Hg2+ exposure of plants was reduced. Enhanced Hg2+ resistance was indicated by improved photosynthesis and growth despite promoted xylem loading and transport of Hg2+, resulting in its accumulation in aboveground tissues that is essential for Hg2+ removal. These results indicate that iron addition has a great potential to improve the growth of R. pseudoacacia in Hg-contaminated soil and promote the accumulation of Hg2+ in aboveground tissues for phytoremediation approaches.},
}

@article {pmid39697845,
year = {2024},
author = {Singkronart, K and Sun, JA and Shamsuddin, SR and Lee, KY},
title = {Upgrading Mixed Plastic Waste through Industrial Symbiosis: Pseudoductile Regenerated Cellulose Fiber-Reinforced Shredder Residue Composites.},
journal = {ACS applied polymer materials},
volume = {6},
number = {23},
pages = {14598-14607},
pmid = {39697845},
issn = {2637-6105},
abstract = {The mechanical performance of mixed plastic waste from shredder residue is hindered by brittleness and catastrophic failure, limiting its potential applications. In this study, the mechanical properties of mixed plastic is enhanced by reinforcement with rayon fibers through a wet powder impregnation process to leverage the fiber's ductility and entanglement. However, mixed plastic remains poorly dispersed in water during the composite manufacturing, resulting in poorly consolidated composite, which further deteriorates the mechanical properties of mixed plastic from 1.5% strain-at-break to 0.7%. To address this issue, the addition of sodium dodecyl sulfate (SDS) surfactant is explored, where the optimal concentration is found beyond the critical micelle concentration at 10 mM. Lowering the surface tension of water and the adsorption of the SDS on the mixed plastic powder surface facilitated homogeneous dispersion of mixed plastic particles, resulting in well-consolidated rayon fiber-reinforced composites. The 30 wt % rayon fiber-reinforced mixed plastic composite prepared with SDS demonstrated a progressive failure behavior, exhibiting a strain-at-break of 8% and a remarkable 350% increase in impact strength compared to unreinforced mixed plastic. This approach provides a platform to overcome the inherent limitations of mixed plastic waste, offering waste-derived plastic alternatives and reducing the need for fossil-derived virgin materials for a wide range of noncritical applications.},
}

@article {pmid39695619,
year = {2024},
author = {Cao, J and Zhou, Y and Tian, T and Ji, J and Deng, Y and Guan, Y and Qi, Y and Wang, L and Wang, L and Huang, Y and Fan, Q and Duanmu, D},
title = {Type-B response regulator RRB12 regulates nodule formation in Lotus japonicus.},
journal = {BMC biology},
volume = {22},
number = {1},
pages = {293},
pmid = {39695619},
issn = {1741-7007},
support = {31870220//National Natural Science Foundation of China/ ; },
mesh = {*Lotus/genetics/microbiology ; *Plant Proteins/genetics/metabolism ; *Root Nodules, Plant/microbiology/metabolism/growth & development/genetics ; *Cytokinins/metabolism ; Gene Expression Regulation, Plant ; Symbiosis ; Plant Root Nodulation/genetics ; },
abstract = {BACKGROUND: The mutualistic beneficial relationship between legume plants and rhizobia enables the growth of plants in nitrogen-limiting conditions. Rhizobia infect legumes through root hairs and trigger nodule organogenesis in the cortex. The plant hormone cytokinin plays a pivotal role in regulating both rhizobial infection and the initiation of nodule development. However, the mechanism used by the cytokinin output module to control symbiosis remains poorly documented.

RESULTS: In this study, we identified a cytokinin signaling output component encoded by the Type-B RESPONSE REGULATOR (RRB) gene, LjRRB12, which is expressed in Lotus japonicus nodule primordia and young nodules. Disruption of LjRRB12 leads to a reduction in nodulation and to an increase in the number of infection threads. Overexpression of LjRRB12[D76E], an active form of the LjRRB12 protein, induces nodule-like structures in wild type and hit1 (hyperinfected 1/lotus histidine kinase 1) mutants but not in nin2 (nodule inception 2) mutants. Additionally, we utilized nCUT&Tag and EMSA to demonstrate that LjRRB12 can bind a CE (cytokinin response element) from the LjNIN promoter.

CONCLUSIONS: Our results provide a deeper understanding of nodule organogenesis by establishing a link between the cytokinin signal and the transcriptional regulation of LjNIN.},
}

*Lotus/genetics/microbiology
*Plant Proteins/genetics/metabolism
*Root Nodules, Plant/microbiology/metabolism/growth & development/genetics
*Cytokinins/metabolism
Gene Expression Regulation, Plant
Symbiosis
Plant Root Nodulation/genetics

@article {pmid39695182,
year = {2024},
author = {Bell, CJ and Sena, JA and Fajardo, DA and Lavelle, EM and Costa, MA and Herman, B and Davin, LB and Lewis, NG and Berry, AM},
title = {A root nodule microbiome sequencing data set from red alder (Alnus rubra Bong.).},
journal = {Scientific data},
volume = {11},
number = {1},
pages = {1343},
pmid = {39695182},
issn = {2052-4463},
support = {1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; 1547842//National Science Foundation (NSF)/ ; },
mesh = {*Microbiota ; *Root Nodules, Plant/microbiology ; *Alnus/microbiology ; *High-Throughput Nucleotide Sequencing ; *Symbiosis ; Frankia/genetics ; },
abstract = {There have been frequent reports of more than one strain of the nitrogen-fixing symbiont, Frankia, in the same root nodule of plants in the genus Alnus, but quantitative assessments of their relative contributions have not been made to date. Neither has the diversity of other microbes, having potential functional roles in symbiosis, been systematically evaluated. Alnus rubra root nodule microbiota were studied using Illumina short read sequencing and kmer-based read classification. Single end 76 bp sequencing was done to a median depth of 96 million reads per sample. Reads were assigned to taxa using KrakenUniq, with taxon abundances being estimated using its companion program Bracken. This was the first high resolution study of Alnus root nodules using next generation sequencing (NGS), quantifying multiple Cluster 1 A Frankia strains in single nodules, and in some cases, a Cluster 4 strain. Root nodules were found to contain diverse bacteria, including several genera containing species known to have growth-promoting effects. Evidence was found for partitioning of some bacterial strains in older versus younger lobes.},
}

*Microbiota
*Root Nodules, Plant/microbiology
*Alnus/microbiology
*High-Throughput Nucleotide Sequencing
*Symbiosis
Frankia/genetics

@article {pmid39695176,
year = {2024},
author = {Hargadon, AC and Viliunas, JW and Koehler, S and Thies, AB and Chen, GY and Ladinsky, MS and Kuwabara, J and Avila-Magana, V and Ruby, EG and Tresguerres, M and McFall-Ngai, MJ},
title = {An acidic microenvironment produced by the V-type ATPase of Euprymna scolopes promotes specificity during Vibrio fischeri recruitment.},
journal = {Communications biology},
volume = {7},
number = {1},
pages = {1642},
pmid = {39695176},
issn = {2399-3642},
support = {R37 AI50661//U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)/ ; R37-AI50661//U.S. Department of Health & Human Services | NIH | National Institute of Allergy and Infectious Diseases (NIAID)/ ; R01 GM135254/GM/NIGMS NIH HHS/United States ; R01-GM135254//U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS)/ ; GRFP 1842402//National Science Foundation (NSF)/ ; GRFP//National Science Foundation (NSF)/ ; },
mesh = {*Aliivibrio fischeri/genetics/physiology/enzymology ; Animals ; *Decapodiformes/microbiology ; *Symbiosis ; Hydrogen-Ion Concentration ; Vacuolar Proton-Translocating ATPases/metabolism/genetics ; },
abstract = {Animals often acquire their microbial symbionts from the environment, but the mechanisms underlying how specificity of the association is achieved are poorly understood. We demonstrate that the conserved proton pump, V-type ATPase (VHA), plays a key role in the establishment of the model light-organ symbiosis between the squid Euprymna scolopes and its bacterial partner, Vibrio fischeri. Recruitment of V. fischeri from the surrounding seawater is mediated by juvenile-specific ciliated fields on the organ's surface. These epithelia produce acidic mucus containing antimicrobials with low-pH optima, creating a chemical environment fostering specific recruitment of V. fischeri. We provide evidence that this critical acidic landscape is created by activity of VHA. VHA inhibition abolished epithelial-cell acidity, resulting in increased mucus pH and inefficient symbiont colonization. Thus, VHA provides a mechanistic link between host modulation of microenvironmental acidity, immune function, and selection of microbial symbionts, a strategy for specificity that may govern other symbioses.},
}

*Aliivibrio fischeri/genetics/physiology/enzymology
Animals
*Decapodiformes/microbiology
*Symbiosis
Hydrogen-Ion Concentration
Vacuolar Proton-Translocating ATPases/metabolism/genetics

@article {pmid39693975,
year = {2024},
author = {Bharti, S and Raj, A and Saratale, GD and Romanholo Ferreira, LF and Lucena de Souza, R and Mulla, SI and Bharagava, RN},
title = {A critical review on the symbiotic effect of bacteria and microalgae on treatment of sewage with biofertilizer production.},
journal = {Journal of environmental management},
volume = {373},
number = {},
pages = {123704},
doi = {10.1016/j.jenvman.2024.123704},
pmid = {39693975},
issn = {1095-8630},
abstract = {Wastes like sewage, kitchen and industrial are the major sources of environmental pollution and health hazards. Sewage contains 99.9% water and 0.1% solid waste including urinal waste and faecal matter alongwith large amounts of nitrate, nitrite, ammonium and phosphate ions. Sewage may also contain a variety of harmful contaminants like analgesics, antihypertensive drugs, antibiotics, dioxin, furans, polychlorinated biphenyls, chlorinated hydrocarbon pesticides, chlorine derivatives and plasticizers etc. making it more harmfull to environment and public health. Hence, sewage must be adequately treated by an effective process before its final discharge into the environment. Biological treatment of sewage is an emerging idea in recent years, which has diverse economic and environmental advantages. Sewage treatment by bacteria and microalgae has numerous advantages as it removes various excessive nutrients from waste with large biomass production and also prevents the utilization of toxic chemicals in conventional treatment process. Microalgae-bacterial biomass have potential to be used as biofertilizers, bio-stimulants and bio-seed primers in agricultural field as these contain various biologically active substances like polysaccharides, carotenoids, free fatty acids, phenols, and terpenoids. This review paper mainly discussing the sewage characteristics and different kinds of organic and inorganic pollutants it contained alongwith its harmfull impacts on environment and public health. It also deals the different conventional as well as emerging treatment technologies and different factors affecting the treatment efficiency. In addition, the utilization of developed microalgal and bacterial biomass as biofertilizer and its effects on crop plant alongwith future prospects has been also discussed in detail.},
}

@article {pmid39691195,
year = {2024},
author = {Banda, MF and Matabane, DL and Munyengabe, A},
title = {A phytoremediation approach for the restoration of coal fly ash polluted sites: A review.},
journal = {Heliyon},
volume = {10},
number = {23},
pages = {e40741},
pmid = {39691195},
issn = {2405-8440},
abstract = {Coal fly ash (CFA) is a predominant waste by-product of coal combustion which is disposed of in open ash dams that utilize large pieces of land. This waste material is classified as a hazardous substance in South Africa as well as in other countries due to its fine particles that are easily blown to the atmosphere and the unacceptable levels of heavy metals and persistent organic pollutants. Contaminants in CFA can pollute surface and ground water, agricultural sites, soil and therefore pose risks to the health of humans and the environment. More than 500 million tons of CFA is produced yearly and over 200 million tons remain unused globally. The production will continue due to high consumer energy demands, especially in countries with heavy reliance on coal for power generation. Despite a significant progress made on the application of phytoremediation approach for decontamination of polluted sites, there is very limited evidence for its potential in the rehabilitation of CFA dumps. Low organic carbon, microbial activities and availability of nutrients including nitrogen contribute to restricted plant growth in CFA, and therefore converting ash dumps to barren lands devoid of vegetation. Leguminous plant species can fix atmospheric nitrogen through symbiotic association with bacteria. Therefore, their intercropping mixture development can improve the chemistry of the substrate and facilitate nutrients availability to the companion plants. This approach can enhance the performance of phytoremediation and promote sustainable practices. The paper provides an overview of the ongoing burden of CFA disposal and discusses the ecological and economic benefits of using legumes, aromatic and bioenergy plants. We identify knowledge gaps to establishing vegetation in ash dumping sites, and provide insights to encourage continued research that will enhance the applicability of phytoremediation in restoration programs.},
}

@article {pmid39690891,
year = {2024},
author = {Fan, W and Liu, K and Xu, Y and Chi, Z},
title = {Solid-state fermentation of corn wet distiller grains and wheat bran with Trichoderma reesei and Candida utilis for improving feed value.},
journal = {Journal of the science of food and agriculture},
volume = {},
number = {},
pages = {},
doi = {10.1002/jsfa.14079},
pmid = {39690891},
issn = {1097-0010},
support = {41861124004//National Natural Science Foundation of China/ ; LJ212410152036//Basic Scientific Research Project of Colleges and Universities of Liaoning Provincial Department of Education/ ; },
abstract = {BACKGROUND: Solid-state fermentation is one of the most effective methods for the high-value utilization of agro-industrial by-products. Co-fermentation of wet distiller grains and agricultural waste is an effective way to mitigate the feed shortage caused by corn consumption for bioethanol. It is still challenging to convert wet distiller grains and wheat bran to easily accessible carbon sources and adjust the balanced proportion of amino acids together by fermentation.

RESULTS: Fermentation time, strain ratio, and the addition of ammonium sulfate have been verified to be the important factors influencing the symbiosis of Trichoderma reesei (T. reesei) and Candida utilis (C. utilis) in a mixed system of wet distiller grains and wheat bran. The optimum conditions were fermentation for 8 days, 2:1 (T. reesei: C. utilis) strain ratio, and addition of 4% ammonium sulfate. After fermentation, the cellulose degradation proportion reached 39.1%, and the hemicellulose degradation proportion was 13.1%. The protein content improved by 29.6%. The lysine content increased by 126%, reaching 11.3 g·kg[-1]. The threonine content increased from 6.10 to 10.3 g·kg[-1]. The phytate content was decreased to 3.97 g·kg[-1]. The in vitro digestibility of dry matter and protein increased to 62.8% and 76.1%, respectively.

CONCLUSIONS: These results indicated the feasibility of improving the feeding value of wet distiller grains and wheat bran by the symbiosis of T. reesei and C. utilis. © 2024 Society of Chemical Industry.},
}

@article {pmid39690548,
year = {2024},
author = {Rusakova, MI and Manzhurova, MI and Zakaryan, AN and Lopatina, SL and Frolova, EF and Raevskiy, KP},
title = {[The gut microbiota in bipolar disorder].},
journal = {Zhurnal nevrologii i psikhiatrii imeni S.S. Korsakova},
volume = {124},
number = {11},
pages = {28-33},
doi = {10.17116/jnevro202412411128},
pmid = {39690548},
issn = {1997-7298},
mesh = {Humans ; *Gastrointestinal Microbiome/physiology ; *Bipolar Disorder/microbiology ; *Brain-Gut Axis/physiology ; Brain/microbiology ; Gastrointestinal Tract/microbiology ; Probiotics/therapeutic use ; },
abstract = {The gut microbiota is a community of microorganisms that live in the digestive tract of living beings and form bidirectional symbiotic relationships with them. It is known that gut bacteria play an important role in maintaining the functioning of the host organism, and disruption in the normal composition of the gut microbiota can contribute to the development of many diseases. The study of microbiota has been gaining popularity in recent years, and its influence on the course of various pathological conditions is becoming more and more undeniable. Due to the growing evidence supporting the connection between the gastrointestinal tract, microbiota and brain, the term «microbiota-gut-brain axis» has appeared. It regulates the functions of the central nervous system, affecting the mood, behavior of the host and, therefore, is involved in the pathogenesis of various mental disorders. Bipolar disorder, a long-known affective mental illness of an endogenous origin, is no exception. The review analyzes the evidence on the relationship between gut microbiota and bipolar disorder, as well as an overview of additional treatments for this disease that affect the microflora of the human body.},
}

Humans
*Gastrointestinal Microbiome/physiology
*Bipolar Disorder/microbiology
*Brain-Gut Axis/physiology
Brain/microbiology
Gastrointestinal Tract/microbiology
Probiotics/therapeutic use

@article {pmid39690524,
year = {2024},
author = {Groover, A and Holbrook, NM and Polle, A and Sala, A and Medlyn, B and Brodersen, C and Pittermann, J and Gersony, J and Sokołowska, K and Bogar, L and McDowell, N and Spicer, R and David-Schwartz, R and Keller, S and Tschaplinski, TJ and Preisler, Y},
title = {Tree drought physiology: critical research questions and strategies for mitigating climate change effects on forests.},
journal = {The New phytologist},
volume = {},
number = {},
pages = {},
doi = {10.1111/nph.20326},
pmid = {39690524},
issn = {1469-8137},
support = {89243022SSC0000//Biological and Environmental Research/ ; //Northern Research Station/ ; 1856450//NSF-IOS/ ; 1029588//USDA NIFA/ ; //New Phytologist Trust/ ; //US Forest Service/ ; //National Science Foundation and the Department of Energy's Next Generation Ecosystem Experiment-Tropics/ ; ERKP886//Center for Bioenergy Innovation (CBI), US Department of Energy, Office of Science, Biological and Environmental Research Program/ ; 2222348//NSF ORCC/ ; },
abstract = {Droughts of increasing severity and frequency are a primary cause of forest mortality associated with climate change. Yet, fundamental knowledge gaps regarding the complex physiology of trees limit the development of more effective management strategies to mitigate drought effects on forests. Here, we highlight some of the basic research needed to better understand tree drought physiology and how new technologies and interdisciplinary approaches can be used to address them. Our discussion focuses on how trees change wood development to mitigate water stress, hormonal responses to drought, genetic variation underlying adaptive drought phenotypes, how trees 'remember' prior stress exposure, and how symbiotic soil microbes affect drought response. Next, we identify opportunities for using research findings to enhance or develop new strategies for managing drought effects on forests, ranging from matching genotypes to environments, to enhancing seedling resilience through nursery treatments, to landscape-scale monitoring and predictions. We conclude with a discussion of the need for co-producing research with land managers and extending research to forests in critical ecological regions beyond the temperate zone.},
}

@article {pmid39690016,
year = {2024},
author = {Guerra-Garcia, FJ and Sankari, S},
title = {NCR peptides in plant-bacterial symbiosis: applications and importance.},
journal = {Trends in microbiology},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.tim.2024.11.012},
pmid = {39690016},
issn = {1878-4380},
abstract = {While establishing symbiotic relationships with nitrogen-fixing soil bacteria certain legumes produce nodule-specific cysteine rich peptides. These peptides turn the bacteria into terminally differentiated non-replicative bacteroids. Here, we discuss the properties, essentiality, emerging clinical and agricultural applications, and the need to study the detailed mechanism of action of these peptides.},
}

@article {pmid39689471,
year = {2024},
author = {Li, M and Chen, H and Wang, M and Zhong, Z and Lian, C and Zhou, L and Zhang, H and Wang, H and Cao, L and Li, C},
title = {Phenotypic plasticity of symbiotic organ highlight deep-sea mussel as model species in monitoring fluid extinction of deep-sea methane hydrate.},
journal = {The Science of the total environment},
volume = {958},
number = {},
pages = {178048},
doi = {10.1016/j.scitotenv.2024.178048},
pmid = {39689471},
issn = {1879-1026},
abstract = {Methane hydrates stored in cold seeps are an important source of energy and carbon for both the endemic chemosynthetic community and humanity. However, the methane fluids may cease and even stop naturally or anthropogenically, calling for a thorough evaluation of its potential impact on the endemic species and local chemosynthetic ecosystems. As one dominant megafauna in cold seeps, some of the deep-sea mussels rely on methanotrophic endosymbionts for nutrition and therefore could serve as a promising model in monitoring the dynamic changes of methane hydrate. However, knowledge on the long-term responses of deep-sea mussels to environmental stresses induced by methane reduction and deprivation, is still lacking. Here, we set up a laboratory system and cultivated methanotrophic deep-sea mussel Gigantidas platifrons without methane supply to survey the phenotypic changes after methane deprivation. While the mussels managed to survive for >10 months after the methane deprivation, drastic changes in the metabolism, function, and development of gill tissue, and in the association with methanotrophic symbionts were observed. In detail, the mussel digested all methanotrophic endosymbionts shortly after methane deprivation for nutrition and remodeled the global metabolism of gill to conserve energy. As the methane deprivation continued, the mussel replaced its bacteriocytes with ciliated cells to support filter-feeding, which is an atavistic trait in non-symbiotic mussels. During the long-term methane deprivation assay, the mussel also retained the generation of new cells to support the phenotypic changes of gill and even promoted the activity after being transplanted back to deep-sea, showing the potential resilience after long-term methane deprivation. Evidences further highlighted the participation of symbiont sterol metabolism in regulating these processes. These results collectively show the phenotypic plasticity of deep-sea mussels and their dynamic responses to methane deprivation, providing essential information in assessing the long-term influence of methane hydrate extinction.},
}

@article {pmid39688607,
year = {2024},
author = {Liapis, CC},
title = {["Pseudoneurotransmission" and gut microbiome - brain communication in neuropsychiatric disorders].},
journal = {Psychiatrike = Psychiatriki},
volume = {},
number = {},
pages = {},
doi = {10.22365/jpsych.2024.024},
pmid = {39688607},
issn = {1105-2333},
abstract = {The gut microbiome, which comprises symbiotic bacteria colonizing the human digestive tract, undergoes dynamic changes during the lifespan, as evidenced by the fact that the number of species and the diversity of their composition decrease significantly with age. The aim of this review is to illuminate bilateral neuroimmunological pathways that determine the role of gut microbiome dysbiosis, not only as a cause but also as a byproduct of many neurodegenerative diseases of the CNS, such as Alzheimer's disease (AD) and Parkinson's disease (PD), but also in the frame of several behavioral and psychiatric pathological conditions such as depressive and anxiety disorders, schizophrenia, and autism spectrum disorder (ASD). Dysbiosis, in particular, reveals a model of "deceptive" mimicry of host molecules that might cause abnormal folding ("misfolding") and pathological aggregation of Aβ-peptide, leading to its dispersion through the gut-brain axis, precipitating microglia cell activation. By controlling myelination at the prefrontal cortex (PFC), a crucial area for multifaceted cognitive behavior, forecasting, and decision-making, the gut/microbiome-brain axis influences mood and social behavior, since major depressive disorder is correlated to white matter disturbance in the PFC, due to disregulations in the expression of myelin-related mRNA in this area. The gut microbiome is altered in psychosis compared to healthy controls, while medication with antipsychotics may result in reduced microbial community diversity. The vagus nerve, as a key element of the parasympathetic nervous system, regulating immune responses, may "detect" gut microbiome metabolites and transfer this intestinal information to the CNS, through its afferents, as in a "pseudo-neurotransmission" process. Scientific interest towards microbiome-based therapies increases as psychobiotics (which are strains of probiotics/prebiotics with specific properties to influence the gut-brain axis) appear to be able to exercise a beneficial effect in many CNS disorders. Lifestyle modifications, such as dietary interventions via psychobiotics intake that might enhance the gut microbiome's ability to produce beneficial metabolites that exert therapeutic effects on intestinal permeability, cognitive function, and immunity, may reveal new research pathways and therapeutic directions leading to a radical change of the "epistemology paradigm" as far as prevention and treatment of major neuro-psychiatric disorders is concerned.},
}

@article {pmid39686544,
year = {2024},
author = {Stott, C and Diop, A and Raymann, K and Bobay, LM},
title = {Co-evolution and Gene Transfers Drive Speciation Patterns in Host-Associated Bacteria.},
journal = {Molecular biology and evolution},
volume = {},
number = {},
pages = {},
doi = {10.1093/molbev/msae256},
pmid = {39686544},
issn = {1537-1719},
abstract = {Microbial communities that maintain symbiotic relationships with animals evolve by adapting to the specific environmental niche provided by their host, yet understanding their patterns of speciation remains challenging. Whether bacterial speciation occurs primarily through allopatric or sympatric processes remains an open question. In addition, patterns of DNA transfers, which are pervasive in bacteria, are more constrained in a closed host-gut system. Eusocial bees have co-evolved with their specialized microbiota for over 85 million years, constituting a simple and valuable system to study the complex dynamics of host-associated microbial interactions. Here we studied the patterns of speciation and evolution of seven specialized gut bacteria from three clades of eusocial bee species: western honey bees, eastern honey bees and bumblebees. We conducted genomic analyses to infer species delineation relative to the patterns of homologous recombination (HR), and horizontal gene transfer (HGT). The studied bacteria presented various modes of evolution and speciation relative to their hosts, but some trends were consistent across all of them. We observed a clear interruption of homologous recombination between bacteria inhabiting different bee hosts, which is consistent with a mechanism of allopatric speciation, but we also identified interruptions of homologous recombination within hosts, suggesting recent or ongoing sympatric speciation. In contrast to HR, we observed that HGT events were not constrained by species borders. Overall, our findings show that in host-associated bacterial populations, patterns of HR and HGT have different impacts on speciation patterns, which are driven by both allopatric and sympatric speciation processes.},
}

@article {pmid39684904,
year = {2024},
author = {Kameli, N and Becker, HEF and Jonkers, DM and Penders, J and Savelkoul, P and Stassen, F},
title = {Investigating the Immunomodulatory Impact of Fecal Bacterial Membrane Vesicles and Their IgA Coating Patterns in Crohn's Disease Patients.},
journal = {International journal of molecular sciences},
volume = {25},
number = {23},
pages = {},
pmid = {39684904},
issn = {1422-0067},
mesh = {Humans ; *Crohn Disease/immunology/microbiology/pathology/metabolism ; *Immunoglobulin A/immunology/metabolism ; *Feces/microbiology ; Adult ; Male ; Female ; Extracellular Vesicles/immunology/metabolism ; THP-1 Cells ; Tumor Necrosis Factor-alpha/metabolism ; Bacteria/immunology ; Middle Aged ; Immunomodulation ; Interleukin-10/metabolism ; },
abstract = {The human intestinal tract contains trillions of bacteria that coexist in a symbiotic relationship with human cells. Imbalances in this interaction can lead to disorders such as Crohn's disease (CD). Bacteria membrane vesicles (MVs), which are released by almost all bacteria, have been demonstrated to play a crucial role in bacteria-host interactions. In this study, we assessed the physical characterizations, immunomodulatory effects, and IgA interactions of MVs derived from fecal samples of CD patients and healthy controls (HCs). MVs were isolated from the frozen fecal samples using a combination of ultrafiltration and size-exclusion chromatography. Using nanoparticle tracking analysis, we found that the MVs of the CD patients showed a significantly lower concentration compared to those of the HCs. Cryo-transmission electron microscopy revealed the larger size of the MVs in active CD (Ac-CD) compared to the MVs of remission CD (Re-CD) and HCs. Differentiated monocyte THP-1 cells released more TNF-a when exposed to MVs from the HCs compared to the CD patients. On the other hand, the MVs from the HCs and Re-CD patients but not the Ac-CD patients induced more anti-inflammatory IL-10. Intriguingly, bead-based flow cytometry analysis showed that the MVs of the HCs and Re-CD patients were more coated with IgA compared to those of the Ac-CD patients. These results suggest the potential role of MVs in the immunomodulatory impact on the pathophysiology of CD. Moreover, IgA seems to regulate these effects by direct binding, which was not the case for the Ac-CD patients. Finally, the IgA coating patterns of the MVs could be used as an additional disease biomarker, as they can clearly identify the exacerbation status of CD.},
}

Humans
*Crohn Disease/immunology/microbiology/pathology/metabolism
*Immunoglobulin A/immunology/metabolism
*Feces/microbiology
Adult
Male
Female
Extracellular Vesicles/immunology/metabolism
THP-1 Cells
Tumor Necrosis Factor-alpha/metabolism
Bacteria/immunology
Middle Aged
Immunomodulation
Interleukin-10/metabolism

@article {pmid39684404,
year = {2024},
author = {Kisiel, A and Miller, T and Łobodzińska, A and Rybak, K},
title = {Biosynthesis of Phenolic Compounds of Medicago truncatula After Inoculation with Selected PGPR Strains.},
journal = {International journal of molecular sciences},
volume = {25},
number = {23},
pages = {},
pmid = {39684404},
issn = {1422-0067},
support = {RID/SP/0045/2024/01//Minister of Science Poland, "Regional Excellence Initiative"/ ; },
mesh = {*Medicago truncatula/microbiology/metabolism/genetics ; *Phenols/metabolism ; *Phenylalanine Ammonia-Lyase/metabolism/genetics ; *Gene Expression Regulation, Plant ; Pseudomonas/metabolism/genetics ; Plant Roots/metabolism/microbiology ; Symbiosis ; Plant Leaves/metabolism/microbiology ; Plant Proteins/metabolism/genetics ; Sinorhizobium meliloti/metabolism/genetics ; },
abstract = {The phenylpropanoid biosynthesis pathway is involved in the response of plants to stress factors, including microorganisms. This paper presents how free-living strains of rhizobacteria Pseudomonas brassicacearum KK5, P. corrugata KK7, Paenibacillus borealis KK4, and the symbiotic strain Sinorhizobium meliloti KK13 affect the expression of genes encoding phenylalanine ammonia-lyase (PAL), the activity of this enzyme, and the production of phenolic compounds in Medicago truncatula. Seedlings were inoculated with rhizobacteria, then at T0, T24, T72, and T168 after inoculation, the leaves and roots were analyzed for gene expression, enzyme activity, and the content of phenolic compounds. All bacteria affected PAL gene expression, in particular, MtPAL2, MtPAL3, and MtPAL4. Pseudomonas strains had the greatest impact on gene expression. The inoculation affected PAL activity causing it to increase or decrease. The most stimulating effect on enzyme activity was observed 168 h after inoculation. A varied effect was also observed in the case of the content of phenolic compounds. The greatest changes were observed 24 h after inoculation, especially with the KK7 strain. The influence of the studied rhizobacteria on the biosynthesis of phenolic compounds at the molecular level (expression of MtPAL genes) and biochemical level (PAL activity and content of phenolic compounds) was confirmed. The MtPAL3 gene underwent the most significant changes after inoculation and can be used as a marker to assess the interaction between M. truncatula and rhizobacteria. The Pseudomonas strains had the greatest influence on the biosynthesis pathway of phenolic compounds.},
}

*Medicago truncatula/microbiology/metabolism/genetics
*Phenols/metabolism
*Phenylalanine Ammonia-Lyase/metabolism/genetics
*Gene Expression Regulation, Plant
Pseudomonas/metabolism/genetics
Plant Roots/metabolism/microbiology
Symbiosis
Plant Leaves/metabolism/microbiology
Plant Proteins/metabolism/genetics
Sinorhizobium meliloti/metabolism/genetics

@article {pmid39683232,
year = {2024},
author = {Gorshkov, AP and Kusakin, PG and Vorobiev, MG and Tsyganova, AV and Tsyganov, VE},
title = {Effect of Insecticides Imidacloprid and Alpha-Cypermethrin on the Development of Pea (Pisum sativum L.) Nodules.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {23},
pages = {},
pmid = {39683232},
issn = {2223-7747},
support = {№ 075-15-2022-320 date 20 April 2022//the Ministry of Science and Higher Education of the Russian Federation/ ; },
abstract = {Insecticides are used commonly in agricultural production to defend plants, including legumes, from insect pests. It is a known fact that insecticides can have a harmful effect on the legume-rhizobial symbiosis. In this study, the effects of systemic seed treatment insecticide Imidor Pro (imidacloprid) and foliar insecticide Faskord (alpha-cypermethrin) on the structural organization of pea (Pisum sativum L.) nodules and their transcriptomic activity were investigated. The plants were treated as recommended by the manufacturer (10 mg/mL for Imidor Pro and 50 µg/mL for Faskord) and twofold concentrations were used for both insecticides. Insecticides had no visible effect on the growth of pea plants. The nodules also showed no visible changes, except for the variant treated with twofold concentration of Imidor Pro. However, the dry weight of shoots and roots differed significantly in insecticide-treated plants compared to untreated plants in almost all treatments. The number of nodules decreased in variants with Imidor Pro treatment. At the ultrastructural level, both insecticides caused cell wall deformation, poly-β-hydroxybutyrate accumulation in bacteroids, expansion of the peribacteroid space in symbiosomes, and inclusions in vacuoles. Treatment with Faskord caused chromatin condensation in nucleus. Imidor Pro treatment caused hypertrophy of infection droplets by increasing the amount of matrix, as confirmed by immunofluorescence analysis of extensins. Transcriptome analysis revealed upregulation of expression of a number of extensin-like protein-coding genes in nodules after the Imidor Pro treatment. Overall, both insecticides caused some minor changes in the legume-rhizobial system when used at recommended doses, but Faskord, an enteric contact insecticide, has fewer negative effects on symbiotic nodules and legume plants; of these two insecticides, it is preferred in pea agricultural production.},
}

@article {pmid39683100,
year = {2024},
author = {Luo, Z and Han, H and Yao, H and Yan, G and Bai, J and Shi, L and Pei, X and Li, J and Li, Q},
title = {Effects of Artificially Modified Microbial Communities on the Root Growth and Development of Tall Fescue in Nutrient-Poor Rubble Soil.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {23},
pages = {},
pmid = {39683100},
issn = {2223-7747},
support = {2021YFB2301605-4//Ministry of Science and Technology of the People's Republic of China, National Key R&D Programme/ ; },
abstract = {The granite rubble soil produced through excavation during construction is nutrient-poor and has a simplified microbial community, making it difficult for plants to grow and increasing the challenges of ecological restoration. Recent studies have demonstrated that microbial inoculants significantly promote plant growth and are considered a potential factor influencing root development. Microorganisms influence root development either directly or indirectly, forming beneficial symbiotic relationships with plant roots. However, the mechanisms by which microorganisms affect root development and root anatomy, as well as the dynamics of soil microbial communities following the artificial application of microbial inoculants, remain unclear. This experiment utilized granite rubble soil from construction excavation in a pot trial, implementing five different treatment methods. After the fast-growing grass species tall fescue (Festuca arundinacea) was planted, four growth-promoting microbial inoculants-Bacillus subtilis (K), Bacillus amyloliquefaciens (JD), Aspergillus niger (H), and Trichoderma harzianum (HC)-were applied to the soil in the pots. These treatments were compared with a control group (CK) that received no microbial inoculant. At 120 days of plant growth, the composition of the soil microbial community, biomass, root structure, and root anatomy were measured for each treatment group. This analysis aimed to explore the effects of different microbial treatments on the microbial communities and root development of Festuca arundinacea root soil. The study found that the addition of microbial inoculants reduced the number of microbial operational taxonomic units (OTUs) of bacteria and fungi in the soil, affecting both the marker species and their abundance at the phylum level. Additionally, microbial inoculants promoted the development of the tall fescue root structure, increasing metrics such as the total root length, root surface area, root volume, and root-to-shoot ratio per plant. Redundancy analysis (RDA) revealed that the area ratios of various components in the root anatomy of tall fescue's primary roots, such as the root cortex area, stele area, and the number of lateral roots, were influenced by Proteobacteria. Mortierellomycota was found to affect the root epidermis area.},
}

@article {pmid39683068,
year = {2024},
author = {Qiu, C and Jin, X and Zhao, Y and Kuai, P and Lou, Y},
title = {A Nucleotide-Binding Domain Leucine-Rich Repeat Gene Regulates Plant Growth and Defense Against Chewing Herbivores.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {23},
pages = {},
pmid = {39683068},
issn = {2223-7747},
support = {2023A0200904//Major Science and Technology project of Xinjiang/ ; },
abstract = {Plant nucleotide-binding leucine-rich repeat immune receptor genes (NLRs) play an important role in plant defenses against pathogens, pathogenic nematodes, and piercing-sucking herbivores. However, little is known about their functions in plant defenses against chewing herbivores. Here, we identified a plasma membrane-localized coiled-coil-type NLR protein, OsPik-2-like, whose transcript levels were induced by the infestation of rice leaf folder (LF, Cnaphalocrocis medinalis) larvae, and by treatment with mechanical wounding. Knocking out OsPik-2-like in rice increased the LF-induced levels of jasmonic acid (JA) and jasmonoyl-isoleucine (JA-Ile), the activity of trypsin protease inhibitors (TrypPIs), and the basal levels of some flavonoids, which in turn decreased the performance of LF larvae. Moreover, knocking out OsPik-2-like reduced plant growth. These findings demonstrate that OsPik-2-like regulates the symbiosis between rice and LF by balancing plant growth and defense.},
}

@article {pmid39683062,
year = {2024},
author = {Danso Ofori, A and Su, W and Zheng, T and Datsomor, O and Titriku, JK and Xiang, X and Kandhro, AG and Ahmed, MI and Mawuli, EW and Awuah, RT and Zheng, A},
title = {Roles of Phyllosphere Microbes in Rice Health and Productivity.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {23},
pages = {},
pmid = {39683062},
issn = {2223-7747},
support = {2019YFN0010, 2020YFH0161, and 22GJHZ0024//Project of the Science and Technology Department of Sichuan Province/ ; 2021-YF05-02326-SN//Chengdu Science and Technology Bureau/ ; },
abstract = {The phyllosphere, comprising the aerial portions of plants, is a vibrant ecosystem teeming with diverse microorganisms crucial for plant health and productivity. This review examines the functional roles of phyllosphere microorganisms in rice (Oryza sativa), focusing on their importance in nutrient uptake, disease resistance, and growth promotion. The molecular mechanisms underlying these interactions are explored along with their potential applications in enhancing sustainable rice production. The symbiotic relationships between rice plants and their associated microorganisms are highlighted, offering insights into improved agricultural practices. Furthermore, this review addresses the challenges and future developments in translating laboratory findings into practical applications. By synthesizing current research, this comprehensive analysis serves as a valuable resource for leveraging phyllosphere microbes in rice farming and related fields.},
}

@article {pmid39682733,
year = {2024},
author = {Starke, MD and Kapusta, M and Płachno, BJ and Bohdanowicz, J},
title = {Immunolocalization of Extensin and Pectin Epitopes in Liparis loeselii Protocorm and Protocorm-like Bodies.},
journal = {Cells},
volume = {13},
number = {23},
pages = {},
pmid = {39682733},
issn = {2073-4409},
support = {539-D030-B951-22, 531-D230-D847-24, and the Priority Research Area under the Strategic Programme Excellence Initiative at Jagiellonian University.//University of Gdańsk and Jagiellonian University./ ; },
mesh = {*Pectins/metabolism ; *Epitopes/immunology/metabolism ; *Orchidaceae/metabolism/immunology ; Plant Proteins/metabolism ; Cell Wall/metabolism ; Glycoproteins/metabolism ; },
abstract = {Liparis loeselii (L.) Rich, an endangered member of the Orchidaceae family, is found in alkaline fens. With the declining populations of L. loeselii, there is a pressing need to reintroduce this species in Central Europe. As in vitro germination is a crucial tool for obtaining plants for introduction into the environment, we looked at the morphological changes occurring during the early stages of L. loeselii development in vitro. As the early stages of orchid development, especially the protocorm stage, are thought to be responsible for SAM formation and the initiation of symbiotic association, we focused on cell wall elements whose epitopes have been found in similar processes in other species: the extensin and pectin rhamnogalacturonan I (RG-I) side chain epitopes. We addressed the following questions: Does the cell wall of L. loeselii change its composition during the early stages of development, as noted in other species? Are there noticeable similarities in the cell wall to organs of different species whose function is to contact microorganisms? Are there regularities that allow the recognition of individual structures on this basis? Immunolocalization revealed changes in the distribution of certain extensins (JIM11 and JIM20) and RG-I (LM5 and LM6) side chain epitopes. Extensins, a type of cell wall protein, were observed during the initial stages of the formation of PLB and the shoot apical meristem of protocorms and PLBs. RG-I, on the other hand, was found to play a significant role in the development of the protocorm and PLB. In pseudobulbs, which appeared on the protocorms, extensins occurred in their storage part. However, RG-I side chains (1→4)-β-galactans (LM5), and (1→5)-α-L-arabinans (LM6) were not found in pseudobulbs. We revealed that a common feature of protocorms and PLBs was an increased amount of extensins, which were detected with the JIM11 antibody, and pectins, which were detected with the LM5 antibody, that were present together, which may prove helpful in determining the identity of the induced structures and distinguishing them from pseudobulbs. Thus, our study unveiled the role of extensins and RG-I during the growth of protocorms and PLBs. We suggest that PLBs may mimic the wall remodelling that occurs in protocorms, which indicates that using cell wall components is an invitation to be colonised by a fungal partner. However, this needs to be tested in future research. The findings of this research can help interpret future studies on the propagation, acclimatisation, and introduction of L. loeselii into the natural environment.},
}

*Pectins/metabolism
*Epitopes/immunology/metabolism
*Orchidaceae/metabolism/immunology
Plant Proteins/metabolism
Cell Wall/metabolism
Glycoproteins/metabolism

@article {pmid39681770,
year = {2024},
author = {Lin, X and Wang, M and Xie, F and Cheng, Y and Yang, L and Gao, J and Li, W and Zhang, X and Tang, T},
title = {Endophytic Bacteria Enterobacter cloacae PN7 Promotes Biosynthesis and Accumulation of Saponins in Panax notoginseng.},
journal = {Current microbiology},
volume = {82},
number = {1},
pages = {41},
pmid = {39681770},
issn = {1432-0991},
mesh = {*Saponins/biosynthesis/metabolism ; *Panax notoginseng/microbiology ; *Endophytes/metabolism/genetics ; *Enterobacter cloacae/metabolism/genetics ; Plant Roots/microbiology ; Biosynthetic Pathways/genetics ; Symbiosis ; Seedlings/microbiology ; Plant Growth Regulators/metabolism ; },
abstract = {Panax notoginseng is an important Chinese medicinal plant. Saponins are the major bioactive secondary metabolites with a wide range of medicinal and commercial value in P. notoginseng, so it is crucial to develop environmentally friendly methods to increase their production. The symbiotic relationship between endophytic bacteria and host plants offers a sustainable approach to enhance secondary metabolite biosynthesis. In this study, it was reported that the co-cultivation of an endophytic bacterium Enterobacter cloacae PN7, isolated from P. notoginseng and its host plant could greatly promote saponin accumulation in the root of seedlings. After six days of PN7 treatment, the total saponin concentration reached 21.64 mg/g, representing a 2.01-fold increase over the control. Transcriptome sequencing revealed that PN7 induction upregulated key genes in the saponin biosynthetic pathway (including DXS, HMGR, PMK, DS, CYP450, and GTs), modulated 253 plant hormone signaling genes (such as those related to JA, ETH, and ABA), and affected 284 transcription factor genes and 47 ABC transporter genes. Co-expression network analysis identified DEGs related to plant hormone signaling, transcription factors, and ABC transporters in saponin biosynthesis and distribution. The results suggested that JA signaling, mediated by transcription factors, such as bHLH and MYBs, and its interaction with ETH, played crucial roles in saponin biosynthesis. Additionally, potential ABC transporter candidates involved in saponin transport were identified. This study highlights the role of endophytic bacteria in enhancing saponin production in P. notoginseng and opens avenues for further research on microbial-plant interactions in secondary metabolite production.},
}

*Saponins/biosynthesis/metabolism
*Panax notoginseng/microbiology
*Endophytes/metabolism/genetics
*Enterobacter cloacae/metabolism/genetics
Plant Roots/microbiology
Biosynthetic Pathways/genetics
Symbiosis
Seedlings/microbiology
Plant Growth Regulators/metabolism

@article {pmid39681734,
year = {2024},
author = {Rajendran, D and Vinayagam, S and Sekar, K and Bhowmick, IP and Sattu, K},
title = {Symbiotic Bacteria: Wolbachia, Midgut Microbiota in Mosquitoes and Their Importance for Vector Prevention Strategies.},
journal = {Microbial ecology},
volume = {87},
number = {1},
pages = {154},
pmid = {39681734},
issn = {1432-184X},
mesh = {*Wolbachia/physiology ; Animals ; *Symbiosis ; *Gastrointestinal Microbiome ; *Mosquito Vectors/microbiology ; Culicidae/microbiology ; Mosquito Control/methods ; },
abstract = {Mosquito-borne illnesses pose a significant threat to eradication under existing vector management measures. Chemo-based vector control strategies (use of insecticides) raise a complication of resistance and environmental pollution. Biological control methods are an alternative approach to overcoming this complication arising from insecticides. The mosquito gut microbiome is essential to supporting the factors that involve metabolic regulation and metamorphic development (from juvenile to adult), as well as the induction of an immune response. The induced immune response includes the JAK-STAT, IMD, and Toll pathways due to the microbial interaction with the midgut cells (MG cells) that prevent disease transmission to humans. The aforementioned sequel to the review provides information about endosymbiont Wolbachia, which contaminates insect cells, including germline and somatic cytoplasm, and inhibits disease-causing pathogen development and transmission by competing for resources within the cell. Moreover, it reduces the host population via cytoplasmic incompatibility (CI), feminization, male killing, and parthenogenesis. Furthermore, the Cif factor in Wolbachia is responsible for CI induction that produces inviable cells with the translocating systems and the embryonic defect-causing protein factor, WalE1 (WD0830), which manipulates the host actin. This potential of Wolbachia can be used to design a paratransgenic system to control vectors in the field. An extracellular symbiotic bacterium such as Asaia, which is grown in the growth medium, is used to transfer lethal genes within itself. Besides, the genetically transferred symbiotic bacteria infect the wild mosquito population and are easily manifold. So, it might be suitable for vector control strategies in the future.},
}

*Wolbachia/physiology
Animals
*Symbiosis
*Gastrointestinal Microbiome
*Mosquito Vectors/microbiology
Culicidae/microbiology
Mosquito Control/methods

@article {pmid39680902,
year = {2024},
author = {Lipowska, MM and Sadowska, ET and Kohl, KD and Koteja, P},
title = {Experimental Evolution of a Mammalian Holobiont? Genetic and Maternal Effects on the Cecal Microbiome in Bank Voles Selectively Bred for Herbivorous Capability.},
journal = {Ecological and evolutionary physiology},
volume = {97},
number = {5},
pages = {274-291},
doi = {10.1086/732781},
pmid = {39680902},
issn = {2993-7973},
mesh = {Animals ; *Arvicolinae/microbiology/genetics ; *Herbivory ; *Gastrointestinal Microbiome/genetics ; Female ; Cecum/microbiology ; Biological Evolution ; Maternal Inheritance/genetics ; Selective Breeding/genetics ; Male ; Diet/veterinary ; },
abstract = {AbstractMammalian herbivory represents a complex adaptation requiring evolutionary changes across all levels of biological organization, from molecules to morphology to behavior. Explaining the evolution of such complex traits represents a major challenge in biology, as it is simultaneously muddled and enlightened by a growing awareness of the crucial role of symbiotic associations in shaping organismal adaptations. The concept of hologenomic evolution includes the partnered unit of the holobiont, the host with its microbiome, as a selection unit that may undergo adaptation. Here, we test some of the assumptions underlying the concept of hologenomic evolution using a unique experimental evolution model: lines of the bank vole (Myodes [=Clethrionomys] glareolus) selected for increased ability to cope with a low-quality herbivorous diet and unselected control lines. Results from a complex nature-nurture design, in which we combined cross-fostering between the selected and control lines with dietary treatment, showed that the herbivorous voles harbored a cecal microbiome with altered membership and structure and changed abundances of several phyla and genera regardless of the origin of their foster mothers. Although the differences were small, they were statistically significant and partially robust to changes in diet and housing conditions. Microbial characteristics also correlated with selection-related traits at the level of individual variation. Thus, the results support the hypothesis that selection on a host performance trait leads to genetic changes in the host that promote the maintenance of a beneficial microbiome. Such a result is consistent with some of the assumptions underlying the concept of hologenomic evolution.},
}

Animals
*Arvicolinae/microbiology/genetics
*Herbivory
*Gastrointestinal Microbiome/genetics
Female
Cecum/microbiology
Biological Evolution
Maternal Inheritance/genetics
Selective Breeding/genetics
Male
Diet/veterinary

@article {pmid39680761,
year = {2024},
author = {Yang, J and Tan, S and Ge, S and Yang, M and Liu, H and Liu, W and Zhang, K and Zhang, Z and Wang, ZH and Shi, J and Liu, J},
title = {Cyanobacteria-probiotics symbionts for modulation of intestinal inflammation and microbiome dysregulation in colitis.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {121},
number = {52},
pages = {e2403417121},
doi = {10.1073/pnas.2403417121},
pmid = {39680761},
issn = {1091-6490},
support = {Nos. 82172762 82373287 21904119 82073395//| National Natural Science Foundation of China-Henan Joint Fund (-)/ ; },
mesh = {Animals ; *Colitis/microbiology/chemically induced/therapy ; *Gastrointestinal Microbiome ; Mice ; *Probiotics/pharmacology/therapeutic use/administration & dosage ; *Symbiosis ; Inflammation/metabolism ; Bacillus subtilis ; Inflammatory Bowel Diseases/microbiology/therapy ; Disease Models, Animal ; Synechocystis/metabolism ; Cyanobacteria/metabolism ; Mice, Inbred C57BL ; },
abstract = {Inflammatory bowel disease (IBD) is often associated with excessive inflammatory response and highly dysregulated gut microbiota. Traditional treatments utilize drugs to manage inflammation, potentially with probiotic therapy as an adjuvant. However, current standard practices often suffer from detrimental side effects, low bioavailability, and unsatisfactory therapeutic outcomes. Microbial complexes characterized by mutually beneficial symbiosis hold great promise for IBD therapy. Here, we aggregated Synechocystis sp. PCC6803 (Sp) with Bacillus subtilis (BS) by biomimetic mineralization to form cyanobacteria-probiotics symbionts (ASp@BS), which reshaped a healthy immune system and gut microbiota in a murine model of acute colitis. The symbionts exhibited excellent tolerance to the harsh environment of the gastrointestinal tract. Importantly, probiotics within the symbionts created a local anaerobic environment to activate the [NiFe]-hydrogenase enzyme of cyanobacteria, facilitating the production of hydrogen gas (H2) to persistently scavenge elevated reactive oxygen species and alleviate inflammatory factors. The resulting reduced inflammation improves the viability of the probiotics to efficiently regulate the gut microbiota and reshape the intestinal barrier functions. Our research elucidates that ASp@BS leverages the synergistic interaction between Sp and BS to create a therapeutic platform that addresses multiple aspects of IBD, offering a promising and comprehensive solution for IBD treatment.},
}

Animals
*Colitis/microbiology/chemically induced/therapy
*Gastrointestinal Microbiome
Mice
*Probiotics/pharmacology/therapeutic use/administration & dosage
*Symbiosis
Inflammation/metabolism
Bacillus subtilis
Inflammatory Bowel Diseases/microbiology/therapy
Disease Models, Animal
Synechocystis/metabolism
Cyanobacteria/metabolism
Mice, Inbred C57BL

@article {pmid39680257,
year = {2024},
author = {Alam, A and Gabriel-Neumann, E},
title = {Arbuscular mycorrhizal fungi travel the world with harvested underground crops.},
journal = {Mycorrhiza},
volume = {35},
number = {1},
pages = {4},
pmid = {39680257},
issn = {1432-1890},
mesh = {*Mycorrhizae/physiology ; *Crops, Agricultural/microbiology ; *Soil Microbiology ; *Sorghum/microbiology ; United Arab Emirates ; Symbiosis ; },
abstract = {In recent years, the dispersal of potentially invasive plants, animals, and pathogens via international trading routes for fresh agricultural goods has been the subject of intensive research and risk assessment. Comparatively little is known about the potential impact of global food trade on the spreading of symbiotic soil microorganisms, such as arbuscular mycorrhizal (AM) fungi. The present study thus assessed whether internationally traded underground crop harvest products carry AM fungal propagules. Twenty batches of tubers, corms or bulbs originating from eight different countries were sampled and used to inoculate Sorghum bicolor (L.) Moench plants grown in a heat-sterilized, sandy dune soil from the United Arab Emirates (UAE). Results revealed that most of the underground crop harvest products contained AM fungal propagules able to establish AM symbioses in a pot experiment under greenhouse conditions. Though it is likely that most AM fungal propagules attached to harvest products will ultimately be eliminated in the waste or sewage stream, it is well possible that a certain portion would find its way into agricultural or natural ecosystems, e.g., via organic waste disposal or use of kitchen greywater for irrigation. Given the large volumes of underground crops traded worldwide, their impact on AM fungal dispersal and distribution deserves further investigation and assessment of associated risks of adulteration of soil microbial communities.},
}

*Mycorrhizae/physiology
*Crops, Agricultural/microbiology
*Soil Microbiology
*Sorghum/microbiology
United Arab Emirates
Symbiosis

@article {pmid39680049,
year = {2024},
author = {Martinez, K and Stillson, PT and Ravenscraft, A},
title = {Inferior Caballeronia symbiont lacks conserved symbiosis genes.},
journal = {Microbial genomics},
volume = {10},
number = {12},
pages = {},
doi = {10.1099/mgen.0.001333},
pmid = {39680049},
issn = {2057-5858},
mesh = {*Symbiosis/genetics ; Animals ; *Phylogeny ; Heteroptera/microbiology/genetics ; Burkholderiaceae/genetics/classification ; Genome, Bacterial ; Burkholderia/genetics/classification ; },
abstract = {Pentatomomorphan bugs can form symbiotic associations with bacteria belonging to the supergenus Burkholderia sensu lato. This relationship has become a model for understanding environmental symbiont acquisition. Host insects can utilize various symbiont strains from across Burkholderia sensu lato; however, host colonization success and benefits conferred vary by bacterial clade. Therefore, we conducted a meta-analysis aimed at identifying candidate genes that underpin beneficial symbioses within this system. We scanned the entire Burkholderiaceae family for the presence of 17 colonization-associated genes, as well as 88 candidate genes that are differentially expressed during symbiosis. There was no difference in the distribution of the 17 colonization-associated genes between symbiotic (Caballeronia and insect-associated plant beneficial and environmental clade) and non-symbiotic lineages; however, there was a higher prevalence of the 88 candidate genes in the insect symbiont lineages. We subsequently analysed the genomes of nine symbiotic Caballeronia species that confer varying fitness benefits to their insect hosts. One symbiont species was significantly worse, one was significantly better and the remaining seven were intermediate in terms of conferred host fitness benefits. We found that species possessing a higher number of the candidate genes conferred faster host development time. Furthermore, we identified two candidate genes that were missing in the least beneficial species but present in the other eight, suggesting that these genes may be important in modulating symbiont quality. Our study suggests that the mechanisms required for host colonization are broadly distributed across Burkholderiaceae, but the genes that determine symbiont quality are more prevalent in insect-associated species. This work helps to identify genes that influence this highly specialized yet diverse symbiosis between Pentatomomorphan insects and Burkholderiaceae bacteria.},
}

*Symbiosis/genetics
Animals
*Phylogeny
Heteroptera/microbiology/genetics
Burkholderiaceae/genetics/classification
Genome, Bacterial
Burkholderia/genetics/classification

@article {pmid39678913,
year = {2024},
author = {Wang, K and Liu, X and Huang, H and Suo, M and Wang, J and Liu, X and Zhang, J and Chen, X and Li, Z},
title = {A new target for treating intervertebral disk degeneration: gut microbes.},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1452774},
pmid = {39678913},
issn = {1664-302X},
abstract = {Intervertebral disk degeneration (IDD) is a common clinical spinal disease and one of the main causes of low back pain (LBP). Generally speaking, IDD is considered a natural degenerative process with age. However, with the deepening of research, people have discovered that IDD is not only related to age, but also has many factors that can induce and accelerate its progression. In addition, the pathogenesis of IDD remains unclear, resulting in limited traditional treatment methods that cannot effectively prevent and treat IDD. Conservative treatment may lead to patients' dependence on drugs, and the pain relief effect is not obvious. Similarly, surgical treatment is highly invasive, with a longer recovery time and a higher recurrence rate. With the deepening of exploration, people have discovered that intestinal microorganisms are an important symbiotic microbial community in the human body and are closely related to the occurrence and development of various diseases. Changes in intestinal microorganisms and their metabolites may affect the body's inflammatory response, immune regulation, and metabolic processes, thereby affecting the health of the intervertebral disk. In this context, the gut microbiota has received considerable attention as a potential target for delaying or treating IDD. This article first introduces the impact of gut microbes on common distal organs, and then focuses on three potential mechanisms by which gut microbes and their metabolites influence IDD. Finally, we also summarized the methods of delaying or treating IDD by interfering with intestinal microorganisms and their metabolites. Further understanding of the potential mechanisms between intestinal microorganisms and IDD will help to formulate reasonable IDD treatment strategies to achieve ideal therapeutic effects.},
}

@article {pmid39678792,
year = {2024},
author = {Cui, JY and Ma, J and Gao, XX and Sheng, ZM and Pan, ZX and Shi, LH and Zhang, BG},
title = {Unraveling the role of cancer-associated fibroblasts in colorectal cancer.},
journal = {World journal of gastrointestinal oncology},
volume = {16},
number = {12},
pages = {4565-4578},
pmid = {39678792},
issn = {1948-5204},
abstract = {Within the intricate milieu of colorectal cancer (CRC) tissues, cancer-associated fibroblasts (CAFs) act as pivotal orchestrators, wielding considerable influence over tumor progression. This review endeavors to dissect the multifaceted functions of CAFs within the realm of CRC, thereby highlighting their indispensability in fostering CRC malignant microenvironment and indicating the development of CAFs-targeted therapeutic interventions. Through a comprehensive synthesis of current knowledge, this review delineates insights into CAFs-mediated modulation of cancer cell proliferation, invasiveness, immune evasion, and neovascularization, elucidating the intricate web of interactions that sustain the pro-tumor metabolism and secretion of multiple factors. Additionally, recognizing the high level of heterogeneity within CAFs is crucial, as they encompass a range of subtypes, including myofibroblastic CAFs, inflammatory CAFs, antigen-presenting CAFs, and vessel-associated CAFs. Innovatively, the symbiotic relationship between CAFs and the intestinal microbiota is explored, shedding light on a novel dimension of CRC pathogenesis. Despite remarkable progress, the orchestrated dynamic functions of CAFs remain incompletely deciphered, underscoring the need for continued research endeavors for therapeutic advancements in CRC management.},
}

@article {pmid39676474,
year = {2024},
author = {Huang, H and Wang, Q and Yang, Y and Zhong, W and He, F and Li, J},
title = {The mycobiome as integral part of the gut microbiome: crucial role of symbiotic fungi in health and disease.},
journal = {Gut microbes},
volume = {16},
number = {1},
pages = {2440111},
doi = {10.1080/19490976.2024.2440111},
pmid = {39676474},
issn = {1949-0984},
mesh = {Humans ; *Gastrointestinal Microbiome ; *Mycobiome ; *Fungi/physiology/classification ; *Symbiosis ; Animals ; *Dysbiosis/microbiology ; Bacteria/classification/isolation & purification/genetics ; },
abstract = {The gut mycobiome significantly affects host health and immunity. However, most studies have focused on symbiotic bacteria in the gut microbiome, whereas less attention has been given to symbiotic fungi. Although fungi constitute only 0.01%-0.1% of the gut microbiome, their larger size and unique immunoregulatory functions make them significant. Factors like diet, antimicrobials use, and age can disrupt the fungal community, leading to dysbiosis. Fungal-bacterial-host immune interactions are critical in maintaining gut homeostasis, with fungi playing a role in mediating immune responses such as Th17 cell activation. This review highlights methods for studying gut fungi, the composition and influencing factors of the gut mycobiome, and its potential in therapeutic interventions for intestinal and hepatic diseases. We aim to provide new insights into the underexplored role of gut fungi in human health.},
}

Humans
*Gastrointestinal Microbiome
*Mycobiome
*Fungi/physiology/classification
*Symbiosis
Animals
*Dysbiosis/microbiology
Bacteria/classification/isolation & purification/genetics

@article {pmid39675287,
year = {2024},
author = {Yan, G and Luo, X and Huang, B and Wang, H and Xing, Y and Wang, Q},
title = {Imbalance in nitrogen and phosphorus allocation between tree roots and leaves induced by nitrogen addition.},
journal = {The Science of the total environment},
volume = {958},
number = {},
pages = {177925},
doi = {10.1016/j.scitotenv.2024.177925},
pmid = {39675287},
issn = {1879-1026},
abstract = {The allocation of limiting elements, such as nitrogen (N) and phosphorus (P), in plant organs is essential for nutrient cycling between soil and plants (soil-plant nutrient cycling) and functional optimization in plant communities. Unprecedented inputs of anthropogenic N have caused drastic N and P imbalances in terrestrial ecosystems. However, the effects of N addition on the allocation strategies of N and P between plant organs remain unclear. In this study, we conducted a long-term, multilevel N addition experiment to investigate the allocation strategies for N and P in plant leaves and fine roots. We found that N addition significantly increased leaf N concentration, leaf P concentration, and leaf N:P ratios, while significantly decreasing fine root N concentration, fine root P concentration, and fine root N:P ratios. Additionally, we demonstrated a higher proportional increase of N in leaves and a lower proportional decrease of P in fine roots with N addition. Furthermore, our analyses revealed that N addition influenced the allocation of N and P between plant leaves and fine roots through changes in plant growth patterns and nutrient distribution strategies. These changes were driven by a significant increase in soil inorganic N concentration, a decrease in soil N cycling and a reduction in mycorrhizal symbiosis. Our findings suggest that N addition will likely lead to an imbalance between the N and P cycles in temperate forest ecosystems, due to the unequal allocation of N and P between tree roots and leaves. This imbalance may, in turn, have negative implications for the provision of ecosystem services.},
}

@article {pmid39674502,
year = {2024},
author = {Liu, Y and Han, Q and Zhang, J and Zhang, X and Chen, Y and Li, M and Hao, Y and Hong, Y and Tang, R and Ferguson, BJ and Gresshoff, PM and Kuai, J and Zhou, G and Li, X and Ji, H},
title = {Soybean nodulation shapes the rhizosphere microbiome to increase rapeseed yield.},
journal = {Journal of advanced research},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.jare.2024.11.034},
pmid = {39674502},
issn = {2090-1224},
abstract = {INTRODUCTION: Crop rotation, a crucial agricultural practice that enhances soil health and crop productivity, is widely used in agriculture worldwide. Soybeans play a crucial role in crop rotation owing to their nitrogen-fixing ability, which is facilitated by symbiotic bacteria in their root systems. The soybean-rapeseed rotation is an effective agricultural practice in the Yangtze River Basin of China. However, the mechanism underlying the effectiveness of this system remains unknown.

OBJECTIVES: The aim of this study was to decipher the mechanisms by which previous soybean cultivation enhances the growth of subsequent rapeseed.

METHODS: Soybeans with three distinct nodulation genotypes were rotated with rapeseed, and the impact of previous soybean cultivation on subsequent rapeseed growth was evaluated by examining the soybean root secretome and soil rhizosphere microbiome.

RESULTS: Soybean-rapeseed rotation significantly enhanced subsequent rapeseed growth and yield, especially when supernodulating soybean plants were used, which released the most nitrogen into the soil rhizosphere. The differences in soybean nodulation capability led to variations in root exudation, which in turn influenced the bacterial communities in the rhizosphere. Notably, the supernodulating soybean plants promoted Sphingomonadaceae family of bacteria growth by secreting oleic acid and cis-4-hydroxy-D-proline, and further attracted them through cis-4-hydroxy-D-proline. Furthermore, the exogenous application of Sphingomonadaceae bacteria, either alone or in combination with rhizobia, significantly enhanced the growth of rapeseed.

CONCLUSION: Our data definitively demonstrated the crucial role of previous soybean cultivation in enhancing the yield of rapeseed, with the assistance of Sphingomonadaceae bacteria and rhizobia. This study elucidates the role of soybean nodulation in rhizosphere bacterial dynamics, highlighting its importance in sustainable agricultural practices.},
}

@article {pmid39674485,
year = {2024},
author = {Ni, Z and Chen, L and Qian, X and Yong, Y and Wu, M and Yihao, L and Li, J and Wang, Y and Li, L and Shao, Y and Chen, A},
title = {Preliminary characterization of Ramaria botrytoides polysaccharide RB-P1-1 and analysis of its hypoglycemic effects by altering the gut microbiota and metabolites in mice with type 2 diabetes mellitus.},
journal = {International journal of biological macromolecules},
volume = {},
number = {},
pages = {138774},
doi = {10.1016/j.ijbiomac.2024.138774},
pmid = {39674485},
issn = {1879-0003},
abstract = {Gut microbiota has a symbiotic relationship with the host and is closely linked to the development of type 2 diabetes mellitus (T2DM). Polysaccharides are natural bioactive compounds with beneficial effects on T2DM; however, the mechanisms underlying their effects remain unclear. This study investigated the hypoglycemic effects of a purified polysaccharide, RB-P1-1, from Ramaria botrytoides and assessed its association with gut microbiota and metabolite changes using 16S rDNA sequencing and liquid chromatography-mass spectrometry, respectively. Hypoglycemic effects were evaluated after microbial community restoration via fecal microbiota transplantation. RB-P1-1 significantly improved hyperglycemia profiles and reshaped gut microbiota, increasing the abundance of Alistipes, Bacteroides, Ruminococcus, Odoribacter, Akkermansia, and Turicibacter. RB-P1-1 modulated microbiota metabolites associated with hypoglycemic effects, including pyridoxamine, L-histidine, quercetin, 3-phosphonopropionic acid, oleoylethanolamide, 3-ketocholanic acid, 4-phenylbutyric acid, LysoPC(P-16:0/0:0), LysoPC(18:2), and short-chain fatty acids, and altered various metabolic pathways involved in T2DM development. Gut microbiota that showed altered abundance were correlated with metabolites that showed altered concentration. Gut microbiota isolated from the RB-P1-1-treated group alleviated the symptoms associated with T2DM. These results suggest RB-P1-1 is an effective active ingredient in the treatment of T2DM by modulating gut microbiota and metabolites.},
}

@article {pmid39674249,
year = {2024},
author = {Bai, X and Li, J and Jiang, H and Cai, R and He, C and Ren, X and Jiang, B},
title = {Effects of multiple temperature variations on nitrogen removal and microbial community structure in tidal flow constructed wetlands.},
journal = {Environmental research},
volume = {},
number = {},
pages = {120616},
doi = {10.1016/j.envres.2024.120616},
pmid = {39674249},
issn = {1096-0953},
abstract = {Tidal-flow constructed wetlands (TFCWs) provide distinct advantages for nitrogen removal by enhancing microbial activity through dynamic water level fluctuations. However, effects of temperature on nitrogen transformation processes and microbial community dynamics in TFCWs remain unclear. We analyzed the effects of TFCWs on nitrogen transformation and microbial community structure under different temperature conditions (23, 16, 12, and 8 °C) through 140 days of temperature-controlled experiments. The nitrogen removal efficiency was considerably enhanced at 23 °C, with transformation rates for ammonia nitrogen (NH4[+]-N) and total nitrogen (TN) reaching 9.28 ± 0.06 g/m[3]/day and 8.35 ± 0.08 g/m[3]/day, respectively. Conversely, at 8 °C, the nitrogen removal efficiency declined, with NH4[+]-N and TN transformation rates decreasing to 7.38 ± 0.05 g/m[3]/day and 6.78 ± 0.05 g/m[3]/day, respectively. Temperature markedly influenced the microbial diversity and community structure, as evidenced by the considerably higher Shannon diversity indices for bacterial communities at 23 °C (5.12 ± 0.21) compared with those at 8 °C (4.52 ± 0.40). Positive microbial interactions were more prevalent at lower temperatures (12 and 8 °C), leading to stronger symbiotic relationships, although the network complexity diminished. The microbial community composition of taxa such as Firmicutes, Proteobacteria, and Thaumarchaeota exhibited greater resilience at lower temperatures. Changes in dissolved oxygen levels also drove changes in bacterial and archaeal communities. These findings underscore the pivotal role of temperature in regulating ecological function and nitrogen removal efficiency of TFCWs and highlight the importance of accounting for temperature variations in the design and management of wastewater treatment systems.},
}

@article {pmid39673195,
year = {2024},
author = {Heredia-Velásquez, AM and Sarkar, S and Thomas, FW and Baza, AC and Garcia-Pichel, F},
title = {Urea-based mutualistic transfer of nitrogen in biological soil crusts.},
journal = {The ISME journal},
volume = {},
number = {},
pages = {},
doi = {10.1093/ismejo/wrae246},
pmid = {39673195},
issn = {1751-7370},
abstract = {Foundational to establishment and recovery of biocrusts is a mutualistic exchange of carbon for nitrogen between pioneer cyanobacteria, including the widespread Microcoleus vaginatus, and heterotrophic diazotrophs in its "cyanosphere". In other such mutualisms, nitrogen is transferred as amino acids or ammonium, preventing losses through specialized structures, cell apposition or intracellularity. Yet, in the biocrust symbiosis relative proximity achieved through chemotaxis optimizes the exchange. We posited that further partner specificity may stem from using an unusual nitrogen vehicle, urea. We show that representative mutualist M. vaginatus PCC 9802 possesses genes for urea uptake, two ureolytic systems, and the urea cycle, overexpressing only uptake and the rare urea carboxylase/allophanate hydrolase (uc/ah) when in co-culture with mutualist Massilia sp. METH4. In turn, it overexpresses urea biosynthesis, but neither urease nor urea uptake when in co-culture. On nitrogen-free medium, three cyanosphere isolates release urea in co-culture with M. vaginatus but not in monoculture. Conversely, M. vaginatus PCC 9802 grows on urea down to the low micromolar range. In natural biocrusts, urea is at low and stable concentrations that do not support the growth of most local bacteria, but aggregates of mutualists constitute dynamic microscale urea hotspots, and the cyanobacterium responds chemotactically to urea. The coordinated gene co-regulation, physiology of cultured mutualists, distribution of urea pools in nature, and responses of native microbial populations, all suggest that low-concentration urea is likely the main vehicle for interspecies N transfer, helping attain partner specificity, for which the rare high-affinity uc/ah system of Microcoleus. vaginatus is likely central.},
}

@article {pmid39672237,
year = {2024},
author = {Pham, MD and Bui, XT and Vo, TK and Dao, TS and Le, LT and Vo, TD and Huynh, KP and Nguyen, TB and Lin, C and Visvanathan, C},
title = {Microalgae - bacteria based wastewater treatment systems: Granulation, influence factors and pollutants removal.},
journal = {Bioresource technology},
volume = {418},
number = {},
pages = {131973},
doi = {10.1016/j.biortech.2024.131973},
pmid = {39672237},
issn = {1873-2976},
abstract = {Wastewater treatment based on microalgae and bacteria symbiosis is an environmentally friendly, sustainable technology that has attracted attention recently because of its high efficiency in treating pollutants, saving energy, and short-term biomass recovery. Among them, the granular microalgae and bacteria combination emerges with the advantages of rapid gravity settling, good resistance to adverse environmental conditions, outstanding wastewater treatment performance, and easy biomass recovery. This review aims to clarify the microalgal-bacterial granule (MBG) - based process for wastewater treatment. In particular, MBG characteristics, granulation mechanism, and influence factors on the process are also discussed. The review contributes to the knowledge system related to MBG research in recent years, thereby pointing out research gaps that need to be filled in the future.},
}

@article {pmid39671865,
year = {2024},
author = {Wang, J and Gao, Y and Liu, Z and Han, Y and Li, W and Lu, X and Dong, K and Zhen, G},
title = {Enhanced propionate degradation and CO2 electromethanogenesis in an up-flow dual-chamber electrocatalytic anaerobic bioreactor (UF-DC-EAB): Leveraging DIET-mediated syntrophy for microbial stability.},
journal = {Water research},
volume = {272},
number = {},
pages = {122927},
doi = {10.1016/j.watres.2024.122927},
pmid = {39671865},
issn = {1879-2448},
abstract = {Anaerobic digestion faces numerous challenges, including high CO2 content in biogas and volatile fatty acids (such as propionate) accumulation in digestate. To address these issues, an up-flow dual-chamber electrocatalytic anaerobic bioreactor (UF-DC-EAB) was developed to enhance propionate degradation through microbial symbiosis while improving biogas quality via CO2 electromethanogenesis. Under the extreme conditions with propionate as the primary carbon source at 6-h HRT, the UF-DC-EAB achieved a propionate removal efficiency of 72.1 ± 9.4 % and a faradaic efficiency of 25.5 ± 5.1 %. Microbial community analysis revealed an enrichment of acetoclastic methanogens (Methanosarcinales, 5.4 %) and syntrophic propionate-oxidizing bacteria (Syntrophobacterales, 13.9 %) in the anode, which facilitated propionate degradation. In the cathode, hydrogenotrophic methanogens (Methanobacterium, 13.6 %) and electroactive bacteria (Geobacter, 6.2 %) were predominant, further promoting CO2 electromethanogenesis and biogas upgrading. Co-occurrence network and structural equation modeling indicated that the electrocatalytic regulation roused the intrinsic capability of the microbial community to oxidize propionate and provoked the occurrence of direct interspecies electron transfer (DIET) among the enriched functional microorganisms, by regulating the synthesis of key molecules like F420 and cytochrome c in response to propionate-induced changes. The DIET-mediated syntropy increased the net energy output by 212.5 %. This study presents a novel electrochemical system combining CO2 electromethanogenesis with propionate-rich digestate degradation, offering an efficient approach for anaerobic post-treatment.},
}

@article {pmid39671445,
year = {2024},
author = {Ndeko, AB and Diedhiou, AG and Founoune-Mboup, H and Chuma, GB and Mugumaarhahama, Y and Diouf, D and Fall, S and Mushagalusa, GN and Kane, A},
title = {Site climate more than soil properties and topography shape the natural arbuscular mycorrhizal symbiosis in maize and spore density within rainfed maize (Zea mays L.) cropland in the eastern DR Congo.},
journal = {PloS one},
volume = {19},
number = {12},
pages = {e0312581},
pmid = {39671445},
issn = {1932-6203},
mesh = {*Zea mays/microbiology/growth & development ; *Mycorrhizae/physiology ; *Soil Microbiology ; *Soil/chemistry ; *Spores, Fungal ; *Symbiosis ; Climate ; Plant Roots/microbiology ; Democratic Republic of the Congo ; Crops, Agricultural/microbiology/growth & development ; Rhizosphere ; },
abstract = {Rhizosphere microorganisms, particularly arbuscular mycorrhizal fungi (AMF), play a vital role in enhancing sustainable maize production. However, uncertainty persist regarding the influence of climate variables and soil properties on mycorrhizal colonization (MC) of maize and the abundance of AM fungal spores in the field. This study aimed to explore the environmental factors such as site climate variables, soil physicochemical properties and topography and vegetation variable, affecting the natural MC of maize and the density of AMF spores. The study hypothesizes that natural maize mycorrhizal colonization and AMF spore density vary significantly across different sites and agroecological zones. It further posits that climatic and edaphic variables predominantly explain the observed variation in mycorrhizal parameters. To assess the impact of these factors, a field study was conducted in 32 sites across three territories in the province of South Kivu, namely Kabare, Walungu, and Uvira. Rhizospheric soil and maize roots were collected from different sites. Maize MC varied significantly among sites, with Kabare and Walungu showing high colonization rates (52.1% and 44.7%, respectively) compared to Uvira (26.40%). Meanwhile, spore density was significantly higher in Uvira (1331.7 spores g-1 soil) than in Kabare (518.9 spores g-1 soil) and Walungu (468.58 spores g-1 soil). Correlation analysis indicated that maize MC was influenced by site climate and soil properties. The PLS-SEM model demonstrated that 76.5% (R2) of the total variance in maize root MC was explained by climatic variables and soil chemical properties. Compared to soil chemical properties, climate characteristics had a more pronounced impact on maize MC. Maize MC was inversely correlated with temperature, C and available P content, while being directly and positively correlated with altitude, rainfall, and base saturation rate. Furthermore, 68.5% (R2) of the spore density variability of AMF was explained by climatic variables and soil physical properties. Spore density was inversely correlated with sand and clay content, field capacity, rainfall, and altitude, while being positively correlated with temperature. The results of this study indicate that climatic conditions exert a more pronounced influence on the mycorrhizal colonization of maize and the density of AMF spores than soil characteristics.},
}

*Zea mays/microbiology/growth & development
*Mycorrhizae/physiology
*Soil Microbiology
*Soil/chemistry
*Spores, Fungal
*Symbiosis
Climate
Plant Roots/microbiology
Democratic Republic of the Congo
Crops, Agricultural/microbiology/growth & development
Rhizosphere

@article {pmid39671023,
year = {2024},
author = {Yan, H and Wang, E and Xu, X and Wei, GS and Zhang, B},
title = {Dissemination patterns and functional role of a symbiotic bacteria Stenotrophomonas maltophilia in Phytoseiulus persimilis.},
journal = {Experimental & applied acarology},
volume = {94},
number = {1},
pages = {11},
pmid = {39671023},
issn = {1572-9702},
support = {Grant No. 6222052//Beijing Natural Science Foundation/ ; Grant No. 32070402//National Natural Science Foundation of China/ ; },
mesh = {*Stenotrophomonas maltophilia/physiology ; Animals ; *Mites/physiology/microbiology ; *Symbiosis ; Female ; Male ; Pest Control, Biological ; Predatory Behavior ; },
abstract = {Symbiotic bacteria play a crucial role in various facets of host biology and physiology. The development and utilization of symbiotic bacteria in insects show promising potential for enhancing their reproduction, temperature tolerance, resistances to pathogens and insecticides. However, limited research has been conducted on symbiotic bacteria in predatory mites. In Phytoseiulus persimilis, we successfully cultivated a strain of Stenotrophomonas maltophilia, which has been noted for its significant contributions to pathogen control, pesticide and toxin resistance, and nutrition provision in some insect species. To explore the effect of S. maltophilia and its potential application on predatory mites, we fed S. maltophilia to P. persimilis and evaluated the transmission dynamics within mite generations. We examined its impacts on predator fitness and resistances to pesticides, pathogens, and starvation. The results showed that the S. maltophilia content in the offspring increased by 12.91 times when gravid mites were fed with the bacterial solution. P. persimilis that consumed prey treated with S. maltophilia showed a 25.20-fold increase in microbial content. Mating with treated males did not affect microbial levels in females. Moreover, S. maltophilia did not cause any discernible effect on the fitness of P. persimilis, including survival, developmental duration, fecundity, and longevity. Notably, it was found to improve P. persimilis survival following exposure to the pathogen Acaricomes phytoseiuli, resulting in a reduction of mortality by 20% compared to the control. This study serves as a foundational step for further utilization of beneficial microbes to improve the efficacy of predatory mite biological control.},
}

*Stenotrophomonas maltophilia/physiology
Animals
*Mites/physiology/microbiology
*Symbiosis
Female
Male
Pest Control, Biological
Predatory Behavior

@article {pmid39671006,
year = {2024},
author = {Hakalehto, E and Jääskeläinen, A},
title = {Production of Novel Energy Gases in Bioprocesses Using Undefined Mixed Cultures.},
journal = {Advances in biochemical engineering/biotechnology},
volume = {},
number = {},
pages = {},
pmid = {39671006},
issn = {0724-6145},
abstract = {Three phases of matter intermingle in various environments. The phenomena behind these fluctuations provide microbial cultures with beneficial interphase on the borderlines. Correspondingly, a bioreactor broth usually consists of a liquid phase but also contains solid particles, gas bubbles, technical surfaces, and other niches, both on a visible scale and microscopically. The diffusion limitation in the suspension is a remarkable hindrance to the reaction sequence during production. It must be overcome technically. Gas flow into the reactor could serve this purpose, and the outgoing stream or bubbling contains volatile products. The various mixing elements or gas flows should be moderated if shear forces disturb the cell growth, biochemical production, enzymatic activity, or any other crucial biological or physicochemical parameters. The focus is to optimize energy production in the form of liberated gases or their mixtures. Many combustible flows need to get purified, depending on their purpose, for example, for various engines. They provide novel sources for traffic in the air, streets, roads, and waterways, not forgetting space technology dimensions.On the other hand, industrial fuels are often used as mixtures of gases or gases with other substances. This approach may facilitate the utilization of side streams. Also, municipal energy needs can be fulfilled by microbial gases. Microbial mixed cultures could play an essential role in the big picture of sustainable industries, living and agriculture, exhibiting an excessive total effect on societies' multifactorial development. The gas phase is the key to realizing their potential.Gaseous emissions are inherent part of all forms of microbial metabolism, both aerobic and anoxic ones. Carbon dioxide is liberated both in respiration and fermentation, but the microbiota also binds volatile carbon compounds. CO2 is also a raw material for plant cultivation, e.g., in greenhouses or in algal pools which both represent the first steps of food chains. Additionally, they produce biomass to produce energy, biochemicals, nutrition, and soil improvement. Gaseous products of the mixed microbial cultures are valuable sources for energy production as purified gases (e.g., biomethane, biohydrogen) or as mixtures (e.g., bio-hythane, volatiles). These relatively simple molecules also serve as supplies for other hydrocarbons (e.g., methanol). Also, many microbial metabolites serve as fuel sources (e.g., bio-oil) and substrates for further biosynthesis. This versatility of potential technological options in energy making and for industrial processes could offer huge opportunities for green energies and sustainable industries, transportation, or municipalities. In the agriculture sector, the complete recycling also includes the consideration of gas phase. This aspect provides increasing sources for clean food production. Moreover, the chemoautotrophic bacteria, including the archaeal strains, could emanate novel streams of biobased products for human use.The bioprocess always consists of a biological component and a reactor or vessel solution, plus its control and adjustment means. Some project examples are taken up introducing the combinations of these two technological mainstreams, which should be in "symbiosis" for the best results. This novel approach could lead the human activities in industries, agriculture, and municipalities into "no waste" situations. At the same time, new global resources for economically feasible and sustainable raw material sources and processes thereof will emerge. In this novel technological ecosystem, connectivity to biosphere will return and remain our societies on healthy foundations, thanks to the microbes and their communities. This chapter introduces some of the potentials.},
}

@article {pmid39670798,
year = {2024},
author = {Baur, P and Comba, P},
title = {Copper coordination chemistry of the patellamides - cyanobactins in the ascidian-Prochloron symbiosis.},
journal = {Dalton transactions (Cambridge, England : 2003)},
volume = {},
number = {},
pages = {},
doi = {10.1039/d4dt03002h},
pmid = {39670798},
issn = {1477-9234},
abstract = {Prochloron didemni, an obligate symbiont of certain ascidians (sea squirts found in tropical areas), produces various cyclic pseudo-octapeptides in large quantities. These secondary metabolites have attracted the attention of medicinal chemists and, due to their four azol(in)e and four amide donor groups, coordination chemists have become interested in these molecules. The structures of the metal-free macrocycles and their dinuclear copper(II) complexes are known, and solution equilibria, spectroscopic properties and a range of biologically relevant reactions have been studied in detail. However, until recently, the properties of the patellamides and structures of the copper(II) complexes in living systems have not been known unambiguously. These are reviewed in the present Perspective and, as a result, it now is possible to discuss possible biological functions of these species.},
}

@article {pmid39670408,
year = {2025},
author = {Arighi, C and Kim, JD and Lu, Z and Rinaldi, F},
title = {Opportunities and Pitfalls with Large Language Models for Biomedical Annotation.},
journal = {Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing},
volume = {30},
number = {},
pages = {706-710},
pmid = {39670408},
issn = {2335-6936},
mesh = {*Computational Biology ; Humans ; Data Curation/statistics & numerical data ; Natural Language Processing ; Programming Languages ; Biomedical Research/statistics & numerical data ; },
abstract = {Large language models (LLMs) and biomedical annotations have a symbiotic relationship. LLMs rely on high-quality annotations for training and/or fine-tuning for specific biomedical tasks. These annotations are traditionally generated through expensive and time-consuming human curation. Meanwhile LLMs can also be used to accelerate the process of curation, thus simplifying the process, and potentially creating a virtuous feedback loop. However, their use also introduces new limitations and risks, which are as important to consider as the opportunities they offer. In this workshop, we will review the process that has led to the current rise of LLMs in several fields, and in particular in biomedicine, and discuss specifically the opportunities and pitfalls when they are applied to biomedical annotation and curation.},
}

*Computational Biology
Humans
Data Curation/statistics & numerical data
Natural Language Processing
Programming Languages
Biomedical Research/statistics & numerical data

@article {pmid39669778,
year = {2024},
author = {Niaz, K and Rauf, M and Arif, M and Hamayun, M and Gul, H and Hashem, A and Abd Allah, EF and Wu, QS},
title = {Drought-tolerant fungal microbes, Aspergillus oryzae and Aspergillus fumigatus, elevate physiohormonal and antioxidant responses of maize under drought stress.},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1488639},
pmid = {39669778},
issn = {1664-302X},
abstract = {INTRODUCTION: Temporary and extended drought stress accelerates phytohormones and reactive oxygen species (ROS) in plants, however, the fate of the plants under stress is mostly determined by the metabolic and molecular reprogramming, which can be modulated by the application of habitat-adapted fungi that triggers resistance to stress upon symbiotic association.

METHODS: The present research exhibited the exploitation of the newly isolated, drought habitat-adapted fungal endophytic consortium of SAB (Aspergillus oryzae) and CBW (Aspergillus fumigatus), on maize under drought stress. SAB and CBW primarily hosted the root tissues of Conyza bonariensis L., which have not been reported earlier, and sufficiently produced growth-promoting metabolites and antioxidants.

RESULTS: SAB and CBW adeptly inhabited the maize roots. They promoted biomass, primary metabolites, osmolytes (protein, sugar, lipids, proline, phenolics, flavonoids), and IAA production while reducing tannins, ABA, and H2O2 contents and increasing antioxidant enzyme activities. In addition, the enhanced adventitious root development at the root/stem interface, and elongated main root development optimum stomatal activity of SAB- and CBW-inoculated maize plants were observed under drought stress. SAB and CBW modulated the expression of the ZmBSK1, ZmAPX, and ZmCAT1 genes in the maize shoot and root tissues under drought stress vs. control, signifying an essential regulatory function for SAB/CBW-induced drought stress tolerance via phytohormonal signaling pathway leading to the antioxidant upregulation.

DISCUSSION: These findings imply that the exogenous administration of the SAB/CBW consortium might be a rather efficient strategy that contributes to optimizing the physio-hormonal attributes and antioxidant potential to alleviate the drought stress in maize.},
}

@article {pmid39669148,
year = {2024},
author = {Olanrewaju, OS and Glick, BR and Babalola, OO},
title = {Beyond correlation: Understanding the causal link between microbiome and plant health.},
journal = {Heliyon},
volume = {10},
number = {23},
pages = {e40517},
pmid = {39669148},
issn = {2405-8440},
abstract = {Understanding the causal link between the microbiome and plant health is crucial for the future of crop production. Established studies have shown a symbiotic relationship between microbes and plants, reshaping our knowledge of plant microbiomes' role in health and disease. Addressing confounding factors in microbiome study is essential, as standardization enables precise identification of microbiome features that influence outcomes. The microbiome significantly impacts plant development, necessitating holistic investigation for maintaining plant health. Mechanistic studies have deepened our understanding of microbiome structure and function related to plant health, though much research still needs to be carried out. This review, therefore, discusses current challenges and proposes advancing studies from correlation to causation and translation. We explore current knowledge on the microbiome and plant health, emphasizing multi-omics approaches and hypothesis-driven research. Future studies should focus on developing translational research for producing probiotics and prebiotics from biomarkers that regulate the microbiome-plant health connection, promoting sustainable crop production through microbiome applications.},
}

@article {pmid39656753,
year = {2024},
author = {Mykhailenko, A and Zieliński, P and Bednarz, A and Schlyter, F and Andersson, MN and Antunes, B and Borowski, Z and Krokene, P and Melin, M and Morales-García, J and Müller, J and Nowak, Z and Schebeck, M and Stauffer, C and Viiri, H and Zaborowska, J and Babik, W and Nadachowska-Brzyska, K},
title = {Complex genomic landscape of inversion polymorphism in Europe's most destructive forest pest.},
journal = {Genome biology and evolution},
volume = {},
number = {},
pages = {},
doi = {10.1093/gbe/evae263},
pmid = {39656753},
issn = {1759-6653},
abstract = {In many species, polymorphic genomic inversions underlie complex phenotypic polymorphisms and facilitate local adaptation in the face of gene flow. Multiple polymorphic inversions can co-occur in a genome, but the prevalence, evolutionary significance, and limits to complexity of genomic inversion landscapes remain poorly understood. Here, we examine genome-wide genetic variation in one of Europe's most destructive forest pests, the spruce bark beetle Ips typographus, scan for polymorphic inversions, and test whether inversions are associated with key traits in this species. We analyzed 240 individuals from 18 populations across the species' European range and, using a whole-genome resequencing approach, identified 27 polymorphic inversions covering approximately 28% of the genome. The inversions vary in size and in levels of intra-inversion recombination, are highly polymorphic across the species range, and often overlap, forming a complex genomic architecture. We found no support for mechanisms such as directional selection, overdominance and associative overdominance that are often invoked to explain the presence of large inversion polymorphisms in the genome. This suggests that inversions are either neutral or maintained by the combined action of multiple evolutionary forces. We also found that inversions are enriched in odorant receptor genes encoding elements of recognition pathways for host plants, mates, and symbiotic fungi. Our results indicate that the genome of this major forest pest of growing social, political, and economic importance harbors one of the most complex inversion landscapes described to date and raise questions about the limits of intraspecific genomic architecture complexity.},
}

@article {pmid39656643,
year = {2024},
author = {Yanagibashi, T and Ikutani, M and Nagai, T and Arita, M and Watanabe, Y and Nagai, Y and Takatsu, K},
title = {IL-5-producing group 2 innate lymphoid cells promote T cell-independent IgA production in cooperation with eosinophils.},
journal = {International immunology},
volume = {},
number = {},
pages = {},
doi = {10.1093/intimm/dxae070},
pmid = {39656643},
issn = {1460-2377},
abstract = {Intestinal bacteria play a critical role in the regulation of the host immune system and an imbalance in intestinal bacterial composition induces various host diseases. Therefore, maintaining a balance in the intestinal bacterial composition is crucial for health. Immunoglobulin A (IgA), produced through T cell-dependent and T cell-independent (TI) pathways, is essential for host defense against pathogen invasion and maintaining the balance of intestinal symbiotic bacteria. Interleukin (IL)-5 is constitutively produced by group 2 innate lymphoid cells (ILC2s) and plays a critical role in the survival and proliferation of B cells and eosinophils. Here, we show that the role of IL-5-producing ILC2s in intestinal TI IgA production at steady state using TCRα deficient mice. In this mouse model, ILC2s increased fecal TI IgA levels in a non-inflammatory state in an IL-5-dependent manner. The administration of recombinant IL-33 (rIL-33) increased the amount of TI IgA production, accompanied by an increase in the number of IL-5-producing ILC2s in the large intestine. In addition, rIL-33 treatment increased IL-5-dependent IgA+ cells in isolated lymphoid follicles, the site of TI IgA production. Furthermore, eosinophils recruited by ILC2s were required for the maximal production of IgA in the TI pathway. Moreover, IL-5 increased the frequency of TI IgA-binding intestinal bacteria and was involved in the maintenance of intestinal bacterial composition. These findings indicate that IL-5-producing ILC2s together with eosinophils contribute to TI IgA production. In addition to their role in TI IgA production, IL-5-producing ILC2s may contribute to the homeostasis of intestinal commensal bacteria.},
}

@article {pmid39657034,
year = {2024},
author = {Wang, Z and Zhang, S and Liang, J and Chen, H and Jiang, Z and Hu, W and Tang, M},
title = {Rhizophagus irregularis regulates RiCPSI and RiCARI expression to influence plant drought tolerance.},
journal = {Plant physiology},
volume = {},
number = {},
pages = {},
doi = {10.1093/plphys/kiae645},
pmid = {39657034},
issn = {1532-2548},
abstract = {Arbuscular mycorrhizal fungi (AMF) can transfer inorganic nitrogen (N) from the soil to host plants to cope with drought stress, with arginine synthesis and NH4+ transport being pivotal processes. However, the regulatory mechanism underlying these processes remains unclear. Here, we found that drought stress upregulated expression of genes involved in the N transfer pathway and putrescine and glutathione synthesis in the mycorrhizal structures of Rhizophagus irregularis within alfalfa (Medicago sativa) roots, i.e., carbamoyl-phosphate synthase (RiCPSI), arginase (RiCARI), urease (RiURE), ornithine decarboxylase (RiODC), and glutamate-cysteine ligase (RiGCL). Furthermore, we confirmed that RiCPSI is a carbamoyl phosphate synthase. Silencing RiCARI via host-induced gene silencing inhibited arbuscule formation, suppressed putrescine and glutathione synthesis, and altered arginine metabolism within R. irregularis-plant symbiosis, leading to a substantial reduction in the drought tolerance of M. sativa. Conversely, silencing RiCPSI decreased arginine, putrescine, and glutathione synthesis in R. irregularis but did not adversely affect NH4+ transfer from fungi to the host plant and drought tolerance of M. sativa. Interestingly, overexpressing RiCPSI via our host-induced gene overexpressing system enhanced arginine, putrescine, and glutathione synthesis in R. irregularis, reduced arbuscule abundance, and improved drought tolerance of M. sativa. Our findings demonstrate that, under drought stress, R. irregularis-plant symbiosis facilitates improved NH4+ transfer from AMF to the host plant. This is accompanied by increased arginine, putrescine, and glutathione synthesis within R. irregularis, driven by the upregulation of RiCPSI and RiCARI expression in mycorrhizal structures within the roots. These molecular adjustments collectively contribute to enhanced drought tolerance in R. irregularis-plant symbiosis.},
}

@article {pmid39656674,
year = {2024},
author = {Reid, TE and Gifford, ML},
title = {Trichoderma gets by with a little help from Streptomyces: fungal-bacterial symbiosis in plant growth promotion.},
journal = {Journal of experimental botany},
volume = {75},
number = {22},
pages = {6893-6897},
doi = {10.1093/jxb/erae439},
pmid = {39656674},
issn = {1460-2431},
}

@article {pmid39668214,
year = {2024},
author = {Mendoza-Suárez, M and Akyol, TY and Nadzieja, M and Andersen, SU},
title = {Increased diversity of beneficial rhizobia enhances faba bean growth.},
journal = {Nature communications},
volume = {15},
number = {1},
pages = {10673},
pmid = {39668214},
issn = {2041-1723},
support = {2071-00012B//Innovationsfonden (Innovation Fund Denmark)/ ; NNF23OC0081220//Novo Nordisk Fonden (Novo Nordisk Foundation)/ ; 771134//EC | Horizon 2020 Framework Programme (EU Framework Programme for Research and Innovation H2020)/ ; },
mesh = {*Vicia faba/microbiology/growth & development ; *Symbiosis ; *Rhizobium leguminosarum/genetics/physiology/growth & development/metabolism ; *Nitrogen Fixation ; Root Nodules, Plant/microbiology ; Genotype ; Plant Root Nodulation ; Rhizobium/physiology/genetics ; },
abstract = {Legume-rhizobium symbiosis provides a sustainable nitrogen source for agriculture. Nitrogen fixation efficiency depends on both legume and rhizobium genotypes, but the implications of their interactions for plant performance in environments with many competing rhizobium strains remain unclear. Here, we let 399 Rhizobium leguminosarum complex sv. viciae strains compete for nodulation of 212 faba bean genotypes. We find that the strains can be categorised by their nodule occupancy profiles into groups that show distinct competitive interactions and plant growth-promoting effects. Further, we show that the diversity of strains occupying root nodules affects plant growth and is under plant genetic control. These insights provide a basis for re-designing rhizobium inoculation and plant breeding strategies to enhance symbiotic nitrogen fixation in agriculture.},
}

*Vicia faba/microbiology/growth & development
*Symbiosis
*Rhizobium leguminosarum/genetics/physiology/growth & development/metabolism
*Nitrogen Fixation
Root Nodules, Plant/microbiology
Genotype
Plant Root Nodulation
Rhizobium/physiology/genetics

@article {pmid39667341,
year = {2024},
author = {Bonfante, P},
title = {Fungal-bacterial endosymbiosis: Recreating an ancient symbiotic relationship.},
journal = {Cell host & microbe},
volume = {32},
number = {12},
pages = {2037-2038},
doi = {10.1016/j.chom.2024.10.018},
pmid = {39667341},
issn = {1934-6069},
mesh = {*Symbiosis ; Fungi/physiology ; Bacteria/genetics/classification ; },
abstract = {Fungal-bacterial endosymbioses, the most intimate typology of symbioses, have been described in different taxa of Mucoromycota, an early diverging group of Fungi. In a recent issue of Nature, Giger and colleagues describe how they implanted a Burkolderia-related microbe inside a Mucoromycota fungus, giving rise to a functional and stable endosymbiosis.},
}

*Symbiosis
Fungi/physiology
Bacteria/genetics/classification

@article {pmid39665174,
year = {2024},
author = {Ayala-García, P and Herrero-Gómez, I and Jiménez-Guerrero, I and Otto, V and Moreno-de Castro, N and Müsken, M and Jänsch, L and van Ham, M and Vinardell, JM and López-Baena, FJ and Ollero, FJ and Pérez-Montaño, F and Borrero-de Acuña, JM},
title = {Extracellular Vesicle-Driven Crosstalk between Legume Plants and Rhizobia: The Peribacteroid Space of Symbiosomes as a Protein Trafficking Interface.},
journal = {Journal of proteome research},
volume = {},
number = {},
pages = {},
doi = {10.1021/acs.jproteome.4c00444},
pmid = {39665174},
issn = {1535-3907},
abstract = {Prokaryotes and eukaryotes secrete extracellular vesicles (EVs) into the surrounding milieu to preserve and transport elevated concentrations of biomolecules across long distances. EVs encapsulate metabolites, DNA, RNA, and proteins, whose abundance and composition fluctuate depending on environmental cues. EVs are involved in eukaryote-to-prokaryote communication owing to their ability to navigate different ecological niches and exchange molecular cargo between the two domains. Among the different bacterium-host relationships, rhizobium-legume symbiosis is one of the closest known to nature. A crucial developmental stage of symbiosis is the formation of N2-fixing root nodules by the plant. These nodules contain endocytosed rhizobia─called bacteroids─confined by plant-derived peribacteroid membranes. The unrestricted interface between the bacterial external membrane and the peribacteroid membrane is the peribacteroid space. Many molecular aspects of symbiosis have been studied, but the interbacterial and interdomain molecule trafficking by EVs in the peribacteroid space has not been questioned yet. Here, we unveil intensive EV trafficking within the symbiosome interface of several rhizobium-legume dual systems by developing a robust EV isolation procedure. We analyze the EV-encased proteomes from the peribacteroid space of each bacterium-host partnership, uncovering both conserved and differential traits of every symbiotic system. This study opens the gates for designing EV-based biotechnological tools for sustainable agriculture.},
}

@article {pmid39664063,
year = {2024},
author = {Li, L and Cai, F and Guo, C and Liu, Z and Qin, J and Huang, J},
title = {Gut microbiome and NAFLD: impact and therapeutic potential.},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1500453},
pmid = {39664063},
issn = {1664-302X},
abstract = {Non-Alcoholic Fatty Liver Disease (NAFLD) affects approximately 32.4% of the global population and poses a significant health concern. Emerging evidence underscores the pivotal role of the gut microbiota-including bacteria, viruses, fungi, and parasites-in the development and progression of NAFLD. Dysbiosis among gut bacteria alters key biological pathways that contribute to liver fat accumulation and inflammation. The gut virome, comprising bacteriophages and eukaryotic viruses, significantly shapes microbial community dynamics and impacts host metabolism through complex interactions. Similarly, gut fungi maintain a symbiotic relationship with bacteria; the relationship between gut fungi and bacteria is crucial for overall host health, with certain fungal species such as Candida in NAFLD patients showing detrimental associations with metabolic markers and liver function. Additionally, the "hygiene hypothesis" suggests that reduced exposure to gut parasites may affect immune regulation and metabolic processes, potentially influencing conditions like obesity and insulin resistance. This review synthesizes current knowledge on the intricate interactions within the gut microbiota and their associations with NAFLD. We highlight the therapeutic potential of targeting these microbial communities through interventions such as probiotics, prebiotics, and fecal microbiota transplantation. Addressing the complexities of NAFLD requires comprehensive strategies that consider the multifaceted roles of gut microorganisms in disease pathology.},
}

@article {pmid39663708,
year = {2024},
author = {Liu, H and Mei, H and Jiang, H and Jiang, L and Lin, K and Jiang, M and Ding, N and Li, X and Gao, Z and Liu, B and Lin, W and Li, J and Zhou, J},
title = {Bioprinted Symbiotic Dressings: A Lichen-Inspired Approach to Diabetic Wound Healing with Enhanced Bioactivity and Structural Integrity.},
journal = {Small (Weinheim an der Bergstrasse, Germany)},
volume = {},
number = {},
pages = {e2407105},
doi = {10.1002/smll.202407105},
pmid = {39663708},
issn = {1613-6829},
support = {//Fundamental Research Funds for the Central Universities/ ; 32271364//National Natural Science Foundation of China/ ; 31971240//National Natural Science Foundation of China/ ; RD-03-202305//Research and Develop Program, West China School of Stomatology, Sichuan University/ ; 2024NSFSC0242//Natural Science Foundation of Sichuan Province/ ; 25NSFSC2494//Natural Science Foundation of Sichuan Province/ ; },
abstract = {Providing oxygen and preventing infection at wound sites are effective ways to heal diabetic chronic wounds. Inspired by natural lichens, a bioprinted biogenic hydrogel (BBH) containing microalgae and probiotics is developed for diabetic chronic wound therapeutics, which offers prolonged biogenetic oxygen supply by microalgae and infection inhibition by probiotics. The rational design of symbiotic BBH with customizable structure and microorganism composition enhances wound resilience against elevated glucose levels and hypoxia, leading to the increased migration ability of fibroblasts and the angiogenic potential of human umbilical vein endothelial cells. Notably, BBH-treated diabetic wounds exhibit dense vascular distribution, reduced hypoxia levels and inflammatory responses, and enhanced epithelial differentiation and keratinization abilities. Consequently, the BBH achieves rapid tissue repairing within 3 d and restores approximately 90% of the whole skin structure within 12 d. This work presents an engineered platform for regulating biological microenvironment of diabetic wounds and provides insights for developing bioprinted hybrid microorganism systems.},
}

@article {pmid39662840,
year = {2024},
author = {Wang, B and Zhang, L and Lian, L and Zhang, X and Qi, Y},
title = {Treatment of Compound Pollution in Simulated Livestock and Poultry Wastewater by Algae-bacteria Symbiosis System.},
journal = {Chemosphere},
volume = {},
number = {},
pages = {143927},
doi = {10.1016/j.chemosphere.2024.143927},
pmid = {39662840},
issn = {1879-1298},
abstract = {Livestock and poultry breeding wastewater contains a large number of heavy metals and antibiotics; the volume is huge, and it is difficult to treat, which causes serious pollution of the environment. Some studies have shown that symbiotic systems can effectively improve the efficiency of sewage treatment, but there is still a lack of research on the treatment of livestock and poultry wastewater. This experiment not only provides a more in-depth discussion of previous studies, but also demonstrates the feasibility of symbiotic treatment of livestock and poultry wastewater and explores the survival mode and operation mechanism of algal and bacterial symbiosis. The results show that the presence of bacteria greatly promoted the growth of microalgae, with production of 0.50-0.59 g/L biomass and 17.5% lipid content. Lipid levels in the algae from the symbiotic system were 1.3 times higher than for the system of pure algae, which is attributed to the bacteria releasing extracellular substances to promote their own growth and providing small molecules of organic matter and other essential elements which can be used by microalgae. In addition, during the removal of complex pollutants in the symbiotic system we found that the main contributor to the removal of heavy metal ions was the adsorption by Chlorella, while the decomposition of antibiotics mainly originated from bacteria. Furthermore, in the context of this experiment was obtained the highest removal rate of SM2 reached 28.8%, while the removal rate of Cu(II) reached 60.6% -66.7%. The technology of symbiotic treatment of wastewater from livestock and poultry breeding fills a gap and lays a theoretical foundation for the improvement of wastewater treatment.},
}

@article {pmid39662569,
year = {2024},
author = {Meng, D and Long, W and Sun, J and Li, H and Wang, Z and Gu, X and Zhang, S},
title = {Eco-friendly fabrication of a delignified wood‑calcium alginate aerogel with improved mechanical properties for efficient thermal insulation and flame retardancy.},
journal = {International journal of biological macromolecules},
volume = {287},
number = {},
pages = {138561},
doi = {10.1016/j.ijbiomac.2024.138561},
pmid = {39662569},
issn = {1879-0003},
abstract = {Wood based composites with low density and great flame retardancy are increasingly required as sustainable and low-carbon building materials for energy conservation. In this work, the symbiosis between bio-based calcium alginate (CaA) and delignified wood was fabricated to form delignified wood-CaA aerogel composites. The density of the delignified wood@CaA sample was dropped to only 89 kg/m[3] from 120 kg/m[3] of the control wood. In addition, its tensile strength, elongation at break, and bending strength reached 16.9 MPa, 4.6 %, and 40.2 MPa, which was increased by 128.4 %, 109.1 %, and 31.8 %, respectively compared to that of control wood. During either heating or cooling, the delignified wood@CaA sample always showed better thermal insulation than the control wood, moreover, it can be developed as an infrared stealthy material. Furthermore, the delignified wood@CaA aerogel reached the limiting oxygen index of 59.2 %, and it was self-extinguished immediately after leaving ignitor in the vertical burning test. In the cone calorimeter test, the total heat release and total smoke production of delignified wood@CaA aerogel decreased by 27.6 % and 71.4 % compared to that of the control wood, respectively. In summary, the light-weighted delignified wood@CaA aerogel composite with superior performance is an ideal material used in sustainable and low-carbon building.},
}

@article {pmid39662506,
year = {2024},
author = {Gribonika, I and Band, VI and Chi, L and Perez-Chaparro, PJ and Link, VM and Ansaldo, E and Oguz, C and Bousbaine, D and Fischbach, MA and Belkaid, Y},
title = {Skin autonomous antibody production regulates host-microbiota interactions.},
journal = {Nature},
volume = {},
number = {},
pages = {},
doi = {10.1038/s41586-024-08376-y},
pmid = {39662506},
issn = {1476-4687},
abstract = {The microbiota colonizes each barrier site and broadly controls host physiology[1]. However, when uncontrolled, microbial colonists can also promote inflammation and induce systemic infection[2]. The unique strategies employed at each barrier tissue to control the coexistence of the host with its microbiota remain largely elusive. Here we uncover that, within the skin, host-microbiota symbiosis depends on the remarkable ability of the skin to act as an autonomous lymphoid organ. Notably, an encounter with a new skin commensal promotes two parallel responses, both under the control of Langerhans cells. On one hand, skin commensals induce the formation of classical germinal centers within the lymph node associated with IgG1 and IgG3 antibody responses. On the other hand, microbial colonization also leads to the development of tertiary lymphoid organs within the skin that can locally sustain IgG2b and IgG2c responses. These phenomena are supported by the ability of regulatory T cells to convert into T follicular helper cells. Skin autonomous production of antibodies is sufficient to control local microbial biomass, as well as subsequent systemic infection with the same microbe. Collectively, these results reveal a striking compartmentalization of humoral responses to the microbiota allowing for control of both microbial symbiosis and potential pathogenesis.},
}

@article {pmid39660940,
year = {2024},
author = {Yurchenko, OV and Chernyshev, AV},
title = {Spermatozoa and Spermatogenesis in the Ribbon Worm Asteronemertes gibsoni (Hoplonemertea, Oerstediidae), a Symbiont of Sea Stars.},
journal = {Journal of morphology},
volume = {285},
number = {12},
pages = {e70014},
doi = {10.1002/jmor.70014},
pmid = {39660940},
issn = {1097-4687},
mesh = {Animals ; *Spermatogenesis/physiology ; Male ; *Spermatozoa/ultrastructure ; *Symbiosis ; Starfish/ultrastructure/physiology ; Invertebrates/ultrastructure/physiology/anatomy & histology ; Acrosome/ultrastructure ; Microscopy, Electron, Transmission ; },
abstract = {In the phylum Nemertea, the class Hoplonemertea (former Enopla) comprises the largest number of studied species with complex spermatozoa. Asteronemertes gibsoni Chernyshev, 1991, a nemertean species having a symbiotic relationship with sea stars, is characterized by complex filiform spermatozoa. Here, spermatogenesis and spermatozoon structure in A. gibsoni have been examined using light and electron microscopy. Numerous proacrosomal vesicles of two kinds have been found in early spermatogenic cells. In spermatozoa, the elongated acrosomal complex consists of two components: a core, which is a spindle-shaped electron-dense acrosomal vesicle with a long anterior end, and its casing of moderate electron density that covers the acrosomal vesicle completely. The acrosomal complex is located laterally relative to the elongated nucleus. The acrosomal casing bears two rows of small, short channels between the nucleus and the electron-dense acrosomal core. In late spermatids, the elongations of the acrosomal complex and the nucleus occur simultaneously and are mediated by numerous microtubules that disappear during the latest stages of spermiogenesis. The flagellum in spermatogenic cells and spermatozoa contains an axoneme with the usual 9 × 2 + 2 microtubular organization and is posteriorly oriented in spermatozoa. As known to date, A. gibsoni has the most modified spermatozoa among investigated Nemertea, and the complex structure of its sperm is suggested to be associated with the reproductive biology, in particular, with fertilization. Additionally, a number of similar ultrastructural features in spermatozoon organization have been found in A. gibsoni and Kurilonemertes phyllospadicola whose phylogenetic relationship was previously proven.},
}

Animals
*Spermatogenesis/physiology
Male
*Spermatozoa/ultrastructure
*Symbiosis
Starfish/ultrastructure/physiology
Invertebrates/ultrastructure/physiology/anatomy & histology
Acrosome/ultrastructure
Microscopy, Electron, Transmission

@article {pmid39659839,
year = {2024},
author = {Fan, X and Su, Z and Zhang, W and Huang, H and He, C and Wu, Z and Zhang, P},
title = {An advanced chitosan based sponges dressing system with antioxidative, immunoregulation, angiogenesis and neurogenesis for promoting diabetic wound healing.},
journal = {Materials today. Bio},
volume = {29},
number = {},
pages = {101361},
pmid = {39659839},
issn = {2590-0064},
abstract = {Promoting wound nerve regeneration and synchronously initiating angiogenesis are critical factors in the healing process of diabetic wounds. However, existing research on diabetic wounds mainly focuses on angiogenesis, bacterial infection and reactive oxygen species, often failing to coordinate neurogenesis and angiogenesis. To coordinate the symbiosis of nerves and blood vessels in the diabetic wounds, we successfully designed a multifunctional chitosan (CS)-based sponges by regulating the structure of CS specifically for diabetic wound healing. This sponge, which facilitates effective exudate transfer and modulates the wound microenvironment, was constructed using hydroxybutyl CS grafted with thioctic acid (TA), named as HCT sponge. When applied in a humid environment, the hydrophobic side chains of the HCT sponge interact with self-assembled hydrophobic domains, forming gel-sponge composite. Experimental results showed that the adhesion strength of the HCT sponge to wet porcine skin was 70.3 kPa. Additionally, the sponge exhibited favorable degradability, cytocompatibility and antioxidant properties. As it is shown in the experiments in vitro, sponge can not only promote cell proliferation, migration, and blood vessel formation, but also promote M2 macrophage polarization. Moreover, the rat liver and femoral artery injury model validated that the HCT sponge can effectively treat heavy bleeding from wounds efficacy through quickly sealing wounds and the formation of multiple hemostatic dams. In vivo studies indicated that the HCT sponge significantly accelerated the diabetic wound healing process compared to the recombinant bovine basic fibroblast growth factor gel, achieving a better recovery from the HCT sponge after 15 days. Pathological results show that the designed novel sponge holds considerable promise for treating diabetic wound, allowing regenerative neurogenesis and angiogenesis at the wound site, which provides a significant potential for further improving clinical applications.},
}

@article {pmid39659293,
year = {2024},
author = {Gasser, MT and Liu, A and Altamia, MA and Brensinger, BR and Brewer, SL and Flatau, R and Hancock, ER and Preheim, SP and Filone, CM and Distel, DL},
title = {Membrane Vesicles Can Contribute to Cellulose Degradation by Teredinibacter turnerae, a Cultivable Intracellular Endosymbiont of Shipworms.},
journal = {Microbial biotechnology},
volume = {17},
number = {12},
pages = {e70064},
pmid = {39659293},
issn = {1751-7915},
support = {//Johns Hopkins University Applied Physics Laboratory/ ; NA19OAR0110303//National Oceanic and Atmospheric Administration/ ; 1R01AI162943-01A1:10062083-NE/NH/NIH HHS/United States ; GBMF9339//Gordon and Betty Moore Foundation/ ; DBI1722553//National Science Foundation/ ; },
mesh = {Animals ; *Symbiosis ; *Cellulose/metabolism ; *Bivalvia/microbiology ; Gammaproteobacteria/metabolism/genetics ; Chromatography, Liquid ; Tandem Mass Spectrometry ; Bacterial Proteins/metabolism/genetics ; Polysaccharides/metabolism ; Carboxymethylcellulose Sodium/metabolism ; },
abstract = {Teredinibacter turnerae is a cultivable cellulolytic Gammaproteobacterium (Cellvibrionaceae) that commonly occurs as an intracellular endosymbiont in the gills of wood-eating bivalves of the family Teredinidae (shipworms). The genome of T. turnerae encodes a broad range of enzymes that deconstruct cellulose, hemicellulose and pectin and contribute to wood (lignocellulose) digestion in the shipworm gut. However, the mechanisms by which T. turnerae secretes lignocellulolytic enzymes are incompletely understood. Here, we show that T. turnerae cultures grown on carboxymethyl cellulose (CMC) produce membrane vesicles (MVs) that include a variety of proteins identified by liquid chromatography-mass spectrometry (LC-MS/MS) as carbohydrate-active enzymes (CAZymes) with predicted activities against cellulose, hemicellulose and pectin. Reducing sugar assays and zymography confirm that these MVs exhibit cellulolytic activity, as evidenced by the hydrolysis of CMC. Additionally, these MVs were enriched with TonB-dependent receptors, which are essential to carbohydrate and iron acquisition by free-living bacteria. These observations indicate a potential role for MVs in lignocellulose utilisation by T. turnerae in the free-living state, suggest possible mechanisms for host-symbiont interaction and may be informative for commercial applications such as enzyme production and lignocellulosic biomass conversion.},
}

Animals
*Symbiosis
*Cellulose/metabolism
*Bivalvia/microbiology
Gammaproteobacteria/metabolism/genetics
Chromatography, Liquid
Tandem Mass Spectrometry
Bacterial Proteins/metabolism/genetics
Polysaccharides/metabolism
Carboxymethylcellulose Sodium/metabolism

@article {pmid39659200,
year = {2024},
author = {Wendlandt, CE and Avelar-Barragan, J and Zomorrodian, AJ and Al-Moussawi, K and Porter, SS and Sachs, JL},
title = {Host control by Acmispon strigosus constrains fitness gains of ineffective Bradyrhizobium symbionts in mixed infections.},
journal = {Journal of evolutionary biology},
volume = {},
number = {},
pages = {},
doi = {10.1093/jeb/voae151},
pmid = {39659200},
issn = {1420-9101},
abstract = {Plant hosts can gain significant growth benefits from symbiosis with microbes, but these benefits could be threatened by divergent fitness interests among partners. Here, we measured fitness outcomes in symbiosis, by varying the genotypes of both microbes and hosts, to examine scenarios that might favor uncooperative symbionts. We studied associations between Acmispon strigosus, an annual legume native to California, and its nitrogen fixing symbionts in the genus Bradyrhizobium. Bradyrhizobium symbionts form root nodules on compatible hosts, with strains varying from effective, fixing substantial nitrogen for the host, to ineffective strains that do not fix nitrogen and provide no benefit to host growth. We co-inoculated four A. strigosus plant lines with nine combinations of effective and ineffective Bradyrhizobium strains and measured the relative fitness of ineffective strains within individual nodules, as hosts must select against uncooperative symbionts to maintain benefits. In mixed infections, ineffective strains always had lower relative fitness in nodules compared to beneficial strains, consistent with efficient punishment of nonfixing rhizobia. However, ineffective strains exhibited genotypic variation in their fitness in nodules within individual nodules co-infected with a beneficial strain, suggesting a role for symbiont competitiveness in shaping this joint phenotype. Variation in symbiont fitness during co-inoculations did not measurably affect plant performance, suggesting that predicted conflict over the joint phenotype of rhizobia fitness has negligible effect on the host.},
}

@article {pmid39658651,
year = {2024},
author = {Garces, KR and Hanley, TC and Deckert, R and Noble, A and Richards, C and Gehring, C and Hughes, AR},
title = {Bacterial and fungal root endophytes alter survival, growth, and resistance to grazing in a foundation plant species.},
journal = {Oecologia},
volume = {207},
number = {1},
pages = {9},
pmid = {39658651},
issn = {1432-1939},
support = {IOS-1556738//National Science Foundation/ ; IOS-1556820//National Science Foundation/ ; IOS-1556087//National Science Foundation/ ; },
mesh = {*Endophytes/physiology ; *Plant Roots/microbiology ; *Herbivory ; Symbiosis ; Fungi/physiology ; Bacteria ; },
abstract = {Plants host an array of microbial symbionts, including both bacterial and fungal endophytes located within their roots. While bacterial and fungal endophytes independently alter host plant growth, response to stress and susceptibility to disease, their combined effects on host plants are poorly studied. To tease apart interactions between co-occurring endophytes on plant growth, morphology, physiology, and survival we conducted a greenhouse experiment. Different genotypes of Spartina alterniflora, a foundational salt marsh species, were inoculated with one bacterial endophyte, Kosakonia oryzae, one fungal endophyte, Magnaporthales sp., or co-inoculated. Within the greenhouse, an unplanned herbivory event occurred which allowed insight into the ways bacteria, fungi, and co-inoculation of both endophytic microbes alters plant defense chemicals and changes herbivory. Broadly, the individual inoculation of the bacterial endophyte increased survival, whereas the fungal endophyte increased plant growth traits. Following the herbivory event, the proportion of stems grazed was reduced when plants were inoculated with the individual endophytes and further reduced when both endophytes were present. Across genotypes, anti-herbivore defense chemicals varied by individual and co-inoculation of endophytes. Bacterial inoculation and genotype interactively affected above:below-ground biomass and S. alterniflora survival of ungrazed plants. Overall, our results highlight the variable outcomes of endophyte inoculation on Spartina growth, morphology, phenolics, and survival. This study furthers our understanding of the combined effects of symbionts and plant multitrophic interactions. Further, exploring intra and inter specific effects of plant--microbe symbiosis may be key in better predicting ecosystem level outcomes, particularly in response to global change.},
}

*Endophytes/physiology
*Plant Roots/microbiology
*Herbivory
Symbiosis
Fungi/physiology
Bacteria

@article {pmid39561350,
year = {2024},
author = {Fajardo, J and Harrison, B and Hervet, VAD and Bakker, MG},
title = {Microbiome profiling suggests novel endosymbiont associations of insect pests of stored grain.},
journal = {Canadian journal of microbiology},
volume = {},
number = {},
pages = {},
doi = {10.1139/cjm-2024-0095},
pmid = {39561350},
issn = {1480-3275},
abstract = {Many arthropods, including economically important pests of stored grains, host intracellular bacterial symbionts. These symbionts can have diverse impacts on host morphology, stress tolerance, and reproductive success. The ability to rapidly determine the infection status of host insects and the identity of intracellular symbionts, if present, is vital to understanding the biology and ecology of these organisms. We used a microbiome profiling method based on amplicon sequencing to rapidly screen 35 captive insect colonies. This method effectively revealed single and mixed infections by intracellular bacterial symbionts, as well as the presence or absence of a dominant symbiont, when that was the case. Because no a priori decisions are required about probable host-symbiont pairing, this method is able to quickly identify novel associations. This work highlights the frequency of endosymbionts, indicates some unexpected pairings that should be investigated further, such as dominant bacterial taxa that are not among the canonical genera of endosymbionts, and reveals different colonies of the same host insect species that differ in the presence and identity of endosymbiotic bacteria.},
}

@article {pmid39658308,
year = {2024},
author = {Taylor, BN},
title = {Symbiotic nitrogen fixation in trees: Patterns, controls, and ecosystem consequences.},
journal = {Tree physiology},
volume = {},
number = {},
pages = {},
doi = {10.1093/treephys/tpae159},
pmid = {39658308},
issn = {1758-4469},
abstract = {Symbiotic nitrogen fixation (SNF) represents the largest natural input of bioavailable nitrogen into the biosphere, impacting key processes spanning from local community dynamics to global patterns of nutrient limitation and primary productivity. While research on SNF historically focused largely on herbaceous and agricultural species, the past two decades have seen major advances in our understanding of SNF by tree species in forest and savanna communities. This has included important developments in the mathematical theory of SNF in forest ecosystems, experimental work on the regulators of tree SNF, broad observational analyses of tree N-fixer abundance patterns, and increasingly process-based incorporation of tree SNF into ecosystem models. This review synthesizes recent work on the local and global patterns, environmental drivers, and community and ecosystem effects of nitrogen-fixing trees in natural ecosystems. By better understanding the drivers and consequences of SNF in forests, this review aims to shed light on the future of this critical process and its role in forest functioning under changing climate, nutrient cycling, and land use.},
}

@article {pmid39658219,
year = {2024},
author = {Juéry, C and Auladell, A and Füssy, Z and Chevalier, F and Yee, DP and Pelletier, E and Corre, E and Allen, AE and Richter, DJ and Decelle, J},
title = {Transportome remodeling of a symbiotic microalga inside a planktonic host.},
journal = {The ISME journal},
volume = {},
number = {},
pages = {},
doi = {10.1093/ismejo/wrae239},
pmid = {39658219},
issn = {1751-7370},
abstract = {Metabolic exchange is one of the foundations of symbiotic associations between organisms and is a driving force in evolution. In the ocean, photosymbiosis between heterotrophic hosts and microalgae is powered by photosynthesis and relies on the transfer of organic carbon to the host (e.g. sugars). Yet, the identity of transferred carbohydrates as well as the molecular mechanisms that drive this exchange remain largely unknown, especially in unicellular photosymbioses that are widespread in the open ocean. Combining genomics, single-holobiont transcriptomics, and environmental metatranscriptomics, we revealed the transportome of the marine microalga Phaeocystis in symbiosis within acantharia, with a focus on sugar transporters. At the genomic level, the sugar transportome of Phaeocystis is comparable to non-symbiotic haptophytes. By contrast, we found significant remodeling of the expression of the transportome in symbiotic microalgae compared to the free-living stage. More particularly, 36% of sugar transporter genes were differentially expressed. Several of them, such as GLUTs, TPTs, and aquaporins, with glucose, triose-phosphate sugars, and glycerol as potential substrates, were upregulated at the holobiont and community level. We also showed that algal sugar transporter genes exhibit distinct temporal expression patterns during the day. This reprogrammed transportome indicates that symbiosis has a major impact on sugar fluxes within and outside the algal cell, and highlights the complexity and the dynamics of metabolic exchanges between partners. This study improves our understanding of the molecular players of the metabolic connectivity underlying the ecological success of planktonic photosymbiosis and paves the way for more studies on transporters across photosymbiotic models.},
}

@article {pmid39658194,
year = {2024},
author = {Takagi, H and Nakamura, Y and Schmidt, C and Kucera, M and Saito, H and Moriya, K},
title = {Two waves of photosymbiosis acquisition in extant planktonic foraminifera explained by ecological incumbency.},
journal = {The ISME journal},
volume = {},
number = {},
pages = {},
doi = {10.1093/ismejo/wrae244},
pmid = {39658194},
issn = {1751-7370},
abstract = {Photosymbiosis, a mode of mixotrophy by algal endosymbiosis, provides key advantage to pelagic life in oligotrophic oceans. Despite its ecological importance, mechanisms underlying its emergence and association with the evolutionary success of photosymbiotic lineages remain unclear. We used planktonic foraminifera, a group of pelagic test-forming protists with an excellent fossil record, to reveal the history of symbiont acquisition among their three main extant clades. We used single-cell 18S rRNA gene amplicon sequencing to reveal symbiont identity and mapped the symbiosis on a phylogeny time-calibrated by fossil data. We show that the highly specific symbiotic interaction with dinoflagellates emerged in the wake of a major extinction of symbiont-bearing taxa at the end of the Eocene. In contrast, less specific and low-light adapted symbioses with pelagophytes emerged 20 million years later, in multiple independent lineages in the Late Neogene, at a time when the vertical structure of pelagic ecosystems was transformed by global cooling. We infer that in foraminifera, photosymbiosis can evolve easily and that its establishment leads to diversification and ecological dominance to such extent, that the proliferation of new symbioses is prevented by the incumbent lineages.},
}

@article {pmid39657996,
year = {2024},
author = {Sakioka, R and Yoneyama, K},
title = {Nitrogen deficiency influences strigolactone levels in basal parts of shoots and shoot branching phenotype in Arabidopsis thaliana.},
journal = {Bioscience, biotechnology, and biochemistry},
volume = {},
number = {},
pages = {},
doi = {10.1093/bbb/zbae185},
pmid = {39657996},
issn = {1347-6947},
abstract = {Strigolactones (SLs) are allelochemicals attracting both symbiotic arbuscular mycorrhizal (AM) fungi and root parasitic plants and a novel class of phytohormones inhibiting lateral shoot branching. In general, nutrient deficiency significantly promotes SL production in roots and exudation into the rhizosphere, and also induces suppressed shoot branching phenotype in host plants of AM fungi including rice, tomato, maize, etc. Nutrient deficiencies also inhibit the shoot branching in Arabidopsis thaliana (hereafter Arabidopsis), a non-host of AM fungi, while the level of carlactone, a non-canonical SL and the SL precursor for the other SLs, was reported to be unaffected. Because Arabidopsis SLs including CL and methyl carlactonoate,, are highly unstable, relationships between SL levels and shoot branching in Arabidopsis remain elusive. Herein, we demonstrate that nitrogen deficiency increases SL levels in the basal part of shoots in Arabidopsis and lateral shoot branching appears to be strongly regulated by these SLs.},
}

@article {pmid39657810,
year = {2024},
author = {Baine, Q and Hughes, DWW and Casares, EE and Martinson, EO and Martinson, VG},
title = {External insect gall morphology influences the functional guilds of natural enemy communities.},
journal = {Proceedings. Biological sciences},
volume = {291},
number = {2036},
pages = {20242424},
pmid = {39657810},
issn = {1471-2954},
support = {//UNM Graduate and Professional Student Association/ ; //University of New Mexico/ ; //UNM Biology Graduate Student Association/ ; },
mesh = {Animals ; *Plant Tumors/parasitology ; *Host-Parasite Interactions ; *Larva/physiology/growth & development/anatomy & histology ; Insecta/physiology ; Biological Evolution ; Wasps/physiology/anatomy & histology ; Symbiosis ; },
abstract = {The evolution of diverse and novel morphological traits is poorly understood, especially how symbiotic interactions can drive these adaptations. The extreme diversity of external traits in insect-induced galls is currently explained by the Enemy Hypothesis, in which these traits have selective advantage in deterring parasitism. While previous tests of this hypothesis used only taxonomic identity, we argue that ecologically functional traits of enemies (i.e. mode of parasitism, larval development strategy) are a crucial addition. Here, we characterize parasitoid guild composition across four disparate gall systems and find consistent patterns of association between enemy guild and gall morphology. Specifically, galls with a longer average larva-to-surface distance host a significantly higher proportion of enemies with a distinct combination of functional traits (i.e. ectoparasitic, idiobiont, elongate ovipositor). Our results support the Enemy Hypothesis and highlight the importance of species ecology in examining insect communities and the evolution of novel defensive characters.},
}

Animals
*Plant Tumors/parasitology
*Host-Parasite Interactions
*Larva/physiology/growth & development/anatomy & histology
Insecta/physiology
Biological Evolution
Wasps/physiology/anatomy & histology
Symbiosis

@article {pmid39657584,
year = {2024},
author = {O'Donnell, RP and Wong, DCJ and Phillips, RD and Peakall, R and Linde, CC},
title = {Discordance Down Under: Combining phylogenomics & fungal symbioses to detangle difficult nodes in a diverse tribe of Australian terrestrial orchids.},
journal = {Systematic biology},
volume = {},
number = {},
pages = {},
doi = {10.1093/sysbio/syae070},
pmid = {39657584},
issn = {1076-836X},
abstract = {Orchid mycorrhizal fungi (OMF) associations in the Orchidaceae are thought to have been a major driver of diversification in the family. In the terrestrial orchid tribe Diurideae, it has long been hypothesised that OMF symbiont associations may reflect evolutionary relationships among orchid hosts. Given that recent phylogenomic efforts have been unable to fully resolve relationships among subtribes in the Diurideae, we sought to ascertain whether orchid OMF preferences may lend support to certain phylogenetic hypotheses. First, we used phylogenomic methods and Bayesian divergence time estimation to produce a genus-level tree for the Diurideae. Next, we synthesised decades of published fungal sequences and morphological/germination data to identify dominant fungal partners at the genus scale and perform ancestral state reconstruction to estimate the evolutionary trajectory of fungal symbiont shifts. Across the tribe, we found phylogenomic discordance stemming from incomplete lineage sorting. However, our results also revealed unprecedented phylogenetic niche conservatism of fungal symbionts within the tribe: entire genera, subtribes, and even groups of related subtribes associate with only a single fungal family, suggesting that fungal symbiont preferences in the Diurideae do indeed reflect phylogenetic relationships among orchid hosts. Moreover, we show that these relationships have evolved directionally from generalist associations with multiple fungal families towards more specific partnerships with only one fungal family. Orchid symbiont preferences here provide new insights into the placement of several groups with longstanding phylogenetic uncertainty. In spite of complex evolutionary histories, host-symbiont relationships can be used to help detangle alternative phylogenetic hypotheses.},
}

@article {pmid39656243,
year = {2024},
author = {Basiru, S and Ait Si Mhand, K and Elfermi, R and Khatour, I and Errafii, K and Legeay, J and Hijri, M},
title = {Enhancing chickpea growth through arbuscular mycorrhizal fungus inoculation: facilitating nutrient uptake and shifting potential pathogenic fungal communities.},
journal = {Mycorrhiza},
volume = {35},
number = {1},
pages = {1},
pmid = {39656243},
issn = {1432-1890},
support = {AS-85//OCP Group/ ; },
mesh = {*Mycorrhizae/physiology ; *Cicer/microbiology/growth & development ; *Soil Microbiology ; Mycobiome ; Glomeromycota/physiology ; Plant Roots/microbiology ; Nutrients/metabolism ; Rhizosphere ; Fungi ; },
abstract = {Arbuscular mycorrhizal fungi (AMF) are the most widespread plant symbionts associated with plant roots, and theyperform numerous functions that contribute to plants' health and physiology. However, there are many knowledge gaps in how the interactions between AMF and root mycobiomes influence the performance of the host plants. To this end, we inoculated a local chickpea cultivar grown in agricultural soil under semi-controlled conditions with Rhizophagus irregularis. In addition to examining mycorrhizal colonization, plant biomass, and mineral nutrition, we sequenced the ITS region of the rDNA to assess the chickpea mycobiome and identify key fungal taxa potentially responding to R. irregularis inoculation. Our results showed that inoculation had a positive effect on chickpea biomass and mineral nutrition, especially the total aboveground phosphorus, potassium and sodium contents. Fusarium, Sporomia, Alternaria, and unknown Pleosporales were the most abundant taxa in the roots, while Stachybotris, Penicillum, Fusarium, Ascobolus, an unknown Pleosporales and Acrophialophora were the most abundant in the rhizosphere. Among the ASVs that either were enriched or depleted in the rhizosphere and roots are potential plant pathogens from the genera Didymella, Fusarium, Neocosmospora, and Stagonosporopsis. This study highlights the relevance of AMF inoculation not only for enhancing chickpea growth and mineral nutrition in semi-arid conditions but also for influencing the composition of the plants' fungal community which contributes to improved plant performance and resilience against biotic and abiotic stress.},
}

*Mycorrhizae/physiology
*Cicer/microbiology/growth & development
*Soil Microbiology
Mycobiome
Glomeromycota/physiology
Plant Roots/microbiology
Nutrients/metabolism
Rhizosphere
Fungi

@article {pmid39656210,
year = {2024},
author = {Ling, X and Guo, H and Di, J and Xie, L and Zhu-Salzman, K and Ge, F and Zhao, Z and Sun, Y},
title = {A complete DNA repair system assembled by two endosymbionts restores heat tolerance of the insect host.},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
volume = {121},
number = {51},
pages = {e2415651121},
doi = {10.1073/pnas.2415651121},
pmid = {39656210},
issn = {1091-6490},
support = {2023YFD1400800//National Key R&D Program of China/ ; no. 32250002//National Natural Science Foundation of China/ ; no. 2023IOZ0307//Initiative Scientific Research of Program, Institute of Zoology, Chinese Academy of Sciences/ ; },
mesh = {Animals ; *Symbiosis ; *Bacterial Proteins/metabolism/genetics ; *DNA Repair ; Buchnera/genetics/metabolism ; Aphids/microbiology/genetics ; Serratia/genetics/metabolism/physiology ; Thermotolerance/genetics ; Promoter Regions, Genetic ; Heat-Shock Response ; },
abstract = {DNA repair systems are essential to maintain genome integrity and stability. Some obligate endosymbionts that experience long-term symbiosis with the insect hosts, however, have lost their key components for DNA repair. It is largely unexplored how the bacterial endosymbionts cope with the increased demand for mismatch repairs under heat stresses. Here, we showed that ibpA, a small heat shock protein encoded by Buchnera aphidicola, directly interacted with the cytoskeletal actin to prevent its aggregation in bacteriocytes, thus reinforcing the stability of bacteriocytes. However, the succession of 11 adenines in the promoter of ibpA is extremely prone to mismatching error, e.g., a single adenine deletion, which impairs the induction of ibpA under heat stress. Coinfection with a facultative endosymbiont Serratia symbiotica remarkably reduced the mutagenesis rate in the Buchnera genome and potentially prevented a single adenine deletion in ibpA promoter, thereby alleviating the heat vulnerability of aphid bacteriocytes. Furthermore, Serratia encoded mutH, a conserved core protein of prokaryotic DNA mismatch repair (MMR), accessed to Buchnera cells, which complemented Buchnera mutL and mutS in constituting an active MMR. Our findings imply that a full complement of a prokaryotic MMR system assembled by two bacterial endosymbionts contributes significantly to the thermostability of aphid bacteriocytes in an ibpA-dependent manner, furnishing a distinct molecular link among tripartite symbioses in shaping resilience and adaptation of their insect hosts to occupy other ecological niches.},
}

Animals
*Symbiosis
*Bacterial Proteins/metabolism/genetics
*DNA Repair
Buchnera/genetics/metabolism
Aphids/microbiology/genetics
Serratia/genetics/metabolism/physiology
Thermotolerance/genetics
Promoter Regions, Genetic
Heat-Shock Response

@article {pmid39656008,
year = {2024},
author = {Ishizaka, A and Tamura, A and Koga, M and Mizutani, T and Yamayoshi, S and Iwatsuki-Horimoto, K and Yasuhara, A and Yamamoto, S and Nagai, H and Adachi, E and Suzuki, Y and Kawaoka, Y and Yotsuyanagi, H},
title = {Dysbiosis of gut microbiota in COVID-19 is associated with intestinal DNA phage dynamics of lysogenic and lytic infection.},
journal = {Microbiology spectrum},
volume = {},
number = {},
pages = {e0099824},
doi = {10.1128/spectrum.00998-24},
pmid = {39656008},
issn = {2165-0497},
abstract = {This study compared intestinal DNA phage dynamics and gut microbiota changes observed at the onset of coronavirus disease 2019 (COVID-19). The study participants included 19 healthy individuals and 19 patients with severe acute respiratory syndrome coronavirus 2 infection. Significant differences were observed in the diversity of the intestinal DNA virome after the onset of COVID-19 compared with that in healthy individuals. Classification by their tail morphology resulted in the order Caudovirales, a double-stranded DNA phage, accounting for >95% of all participants. In classifying phages based on host bacteria, a decreased number of phages infecting mainly the Clostridia class was observed immediately after the onset of COVID-19 and recovered over time. After the onset of COVID-19, two distinct movement patterns of intestinal phages and their host bacteria were observed: phage- and bacteria-predominant. The abundance of obligate anaerobes, such as Clostridium_sense_strict_1, Fusicatenibacter, and Romboutsia, and the phages hosting these bacteria decreased immediately after the onset of COVID-19, and faster phage recovery was observed compared with bacterial recovery. In contrast, the genus Staphylococcus, a facultative anaerobic bacterium, increased immediately after the onset of COVID-19, whereas the phages infecting Staphylococcus decreased. Furthermore, immediately after the onset of COVID-19, the percentage of lytic phages increased, whereas that of temperate phages decreased. These observations suggest that the gut microbiota dysbiosis observed immediately after the onset of COVID-19 may be linked to phage dynamics that control gut microbiota and may also affect the recovery from dysbiosis.IMPORTANCEBacteriophages infect and replicate with bacteria and archaea and are closely associated with intestinal bacteria. The symbiotic relationship between gut microbiota and bacteriophages is of interest, but it is challenging to study their dynamics in the human body over time. SARS-CoV-2 infection has been reported to alter the gut microbiota, which is involved in gut immune regulation and pathophysiology, although changes in the intestinal phages of patients with SARS-CoV-2 and their dynamic relationship with the gut microbiota remain unclear. SARS-CoV-2 infection, which follows a transient pathological course from disease onset to cure, may provide a reliable model to investigate these interactions in the gut environment. Therefore, this study aimed to elucidate the correlation between gut microbiota and intestinal DNA virome dynamics in COVID-19 pathogenesis. This study found that the dysbiosis observed in SARS-CoV-2 infection involves a growth strategy that depends on the phage or bacterial dominance.},
}

@article {pmid39655940,
year = {2024},
author = {Zhang, H and Wang, W and Honnas, L and Mazzola, M and Somera, T},
title = {Evaluating the stability of nursery-established arbuscular mycorrhizal fungal associations in apple rootstocks.},
journal = {Applied and environmental microbiology},
volume = {},
number = {},
pages = {e0193724},
doi = {10.1128/aem.01937-24},
pmid = {39655940},
issn = {1098-5336},
abstract = {Arbuscular mycorrhizal fungi (AMF) are promoted as commercial bioinoculants for sustainable agriculture. Little is known, however, about the survival of AMF inoculants in soil and their impacts on native or pre-established AMF communities in root tissue. The current study was designed to assess the stability of pre-existing/nursery-derived AMF in apple rootstocks after being planted into soil containing a known community of AMF with a limited number of species. Root-associated endophytic communities (bacteria and fungi) are known to differ depending on apple rootstock genotype. Thus, an additional aim of this study was to explore the effect of apple rootstock genotype on AMF community structure. A greenhouse experiment was conducted in which a variety of apple rootstock genotypes (G.890, G.935, M.26, and M.7) were inoculated with a commercially available, multi-species AMF consortium. Nursery-derived AMF communities were sequenced, and changes to AMF community structure following cultivation in pasteurized soil (inoculated and non-inoculated) were assessed using a Glomeromycota-specific phylogenetic tree, which included 91 different AMF species from 24 genera. Results show that inoculant colonization potential was limited and that apple rootstocks serve as a significant source of inoculum from the nursery where they are produced. Rootstocks established relationships with introduced AMF in a genotype-specific manner. Regardless of colonization success, however, the inoculant caused alterations to the resident AMF communities of both Geneva and Malling rootstocks, particularly low abundance taxa. In addition, phylogeny-based analysis revealed a unique, well-supported clade of unknown taxonomy, highlighting the importance of using phylogenetic-based classification for accurate characterization of AMF communities.IMPORTANCEUnderstanding the impacts of introduced AMF on residential AMF communities is essential to improving plant productivity in nursery and orchard systems. In general, there is a dearth of data on the interactions of commercial AMF inoculants with pre-established AMF communities living in symbiosis with the host plant. The interplay between apple rootstock genotype and the endophytic root microbiome is also an area where more research is needed. This study demonstrates the potential for nursery-established AMF associations to be maintained when transplanted into the field. In addition to providing insight into rootstock/AMF associations, our study calls attention to the current issues attendant with relying on web-based databases for determining AMF identity. The use of phylogenetic tools represents one possible solution and may be of value to industry practitioners in terms of improving product composition and consistency.},
}

@article {pmid39655922,
year = {2024},
author = {Gasser, MT and Liu, A and Flatau, R and Altamia, MA and Filone, CM and Distel, DL},
title = {Closing the genome of Teredinibacter turnerae T7902 by long-read nanopore sequencing.},
journal = {Microbiology resource announcements},
volume = {},
number = {},
pages = {e0048424},
doi = {10.1128/mra.00484-24},
pmid = {39655922},
issn = {2576-098X},
abstract = {We present the complete closed circular genome sequence derived from the Oxford Nanopore sequencing of the shipworm endosymbiont, Teredinibacter turnerae T7902 (DSM 15152, ATCC 39867), originally isolated from the shipworm, Lyrodus pedicellatus (1). This sequence will aid in the comparative genomics of shipworm endosymbionts and the understanding of the host-symbiont evolution.},
}

@article {pmid39655918,
year = {2024},
author = {Farrell, MV and Aljaber, AM and Amoruso, M and Chan, WF and Dael, JR and De Tomas, ML and Delavega, EG and Eslava, JM and Holdbrook-Smith, BJ and Lee, P and Mai, V and Michael, LR and Moreno, SV and Quevedo, JF and Roberts, AG and Villanueva, J and Westin, C and Zazueta, DM and Shikuma, NJ},
title = {Draft genome sequences of Flagellimonas sp. MMG031 and Marinobacter sp. MMG032 isolated from the dinoflagellate Symbiodinium pilosum.},
journal = {Microbiology resource announcements},
volume = {},
number = {},
pages = {e0091324},
doi = {10.1128/mra.00913-24},
pmid = {39655918},
issn = {2576-098X},
abstract = {Here, we report the draft genome sequences of Flagellimonas sp. MMG031 and Marinobacter sp. MMG032, isolated from coral-associated dinoflagellate Symbiodinium pilosum, assembled and analyzed by undergraduate students participating in a Marine Microbial Genomics (MMG) course. A genomic comparison suggests MMG031 and MMG032 are novel species and a resource for restoration and biotechnology.},
}

@article {pmid39655670,
year = {2024},
author = {Dong, R and Wang, W and Luo, N and Li, H and Liu, J and Wang, Y and Ye, Y and Zhu, H and Li, F and Yu, H and Cao, Y},
title = {MtNAD1 associates with the autophagy complex to contribute to the degradation of immunity-related proteins in Medicago truncatula nodules.},
journal = {The New phytologist},
volume = {},
number = {},
pages = {},
doi = {10.1111/nph.20336},
pmid = {39655670},
issn = {1469-8137},
support = {32090063//National Natural Science Foundation of China/ ; 31970307//National Natural Science Foundation of China/ ; 32000191//National Natural Science Foundation of China/ ; 2019YFA0904700//National Key R&D Program of China/ ; AML2023B01//National Key Lab of Agricultural Microbiology/ ; 2022A1515011483//Natural Science Foundation of Guangdong Province/ ; 2021A1515011036//Natural Science Foundation of Guangdong Province/ ; },
abstract = {Plant immunity is suppressed in the symbiotic nodule cells, thereby facilitating rhizobial infection. Medicago truncatula NODULES WITH ACTIVATED DEFENSE1 (MtNAD1) is crucial for suppressing immunity in nodules; however, its molecular function is unclear. We explored the molecular basis of the role of MtNAD1 in suppressing innate immunity in M. truncatula nodules. Medicago truncatula mutants lacking MtATG7 produced defective nodules, sharing some similarities with the Mtnad1 mutant nodules. Furthermore, MtNAD1 interacted with several immunity-related proteins, including BAX-inhibitor1a (MtBI-1a), two Lysin-motif proteins (MtLYM1/2), Pathogenesis-related10 (MtPR10c/d), MtMPK3/6, and two Lysin-motif receptor kinases (MtLYK8/9). In addition, MtNAD1 and the autophagy pathway contributed to the reduction of MtBI-1, MtPR10c/d, and MtLYM1/2 protein levels in planta. Knocking out either the MtBI-1 or MtLYM1/2 gene in the M. truncatula nad1 mutant can partially restore the defective nodules of the nad1 mutant. Our results demonstrate that MtNAD1 associates with the autophagy pathway by interacting with MtATG8, contributing to the degradation of several immunity-related proteins in M. truncatula nodules during rhizobial colonization and thereby supporting the development of a successful symbiosis.},
}

@article {pmid39654066,
year = {2024},
author = {Salari, H and Amooaghaie, R and Mozafari, H and Ghorbanpour, M and Sedaghati, E},
title = {Impact of two arbuscular mycorrhizal fungi species on arsenic tolerance and accumulation in safflower (Carthamus tinctorius L.).},
journal = {BMC plant biology},
volume = {24},
number = {1},
pages = {1174},
pmid = {39654066},
issn = {1471-2229},
mesh = {*Mycorrhizae/physiology ; *Carthamus tinctorius/microbiology/metabolism/drug effects ; *Glomeromycota/physiology ; *Arsenic/metabolism/toxicity ; *Soil Pollutants/metabolism/toxicity ; *Biodegradation, Environmental ; Symbiosis ; Plant Roots/microbiology/metabolism/growth & development ; Chlorophyll/metabolism ; Phosphorus/metabolism ; Fungi ; },
abstract = {BACKGROUND: Arbuscular mycorrhizal fungi (AMF) can regulate metal(loid) tolerance in plants and their capacity for phytoremediation. These effects can vary depending on the host plant and the AMF species. The impact of different AMF species on the ability of safflower (Carthamus tinctorius L.) for arsenic (As) phytoremediation is still largely unknown. Therefore, this study aimed to assess the effect of two AMF species, Rhizophagus irregularis, and Funneliformis mosseae, on the tolerance and accumulation of As in safflower in soils spiked with varying arsenate concentrations (0, 25, 50, and 100 mg kg[-1]).

RESULTS: The results indicated that both AMF species established effective symbiotic relationships with safflower. However, plants inoculated with R. irregularis exhibited higher mycorrhizal dependency and root colonization, especially under 100 mg kg[-1] As. Both AMF species significantly improved plant growth parameters, chlorophyll content, and phosphorus (P) nutrition, which resulted in increased P/As ratio and enhanced tolerance index in safflower plants. In addition, AMF inoculation reduced As-induced lipid peroxidation by enhancing catalase and peroxidase activity in leaves and roots. While the mycorrhizal symbiosis didn't affect As availability in soils, it significantly reduced shoot As concentration and the translocation factor under all As levels. Furthermore, mycorrhizal inoculation, especially with R. irregularis, increased As concentration and modified-bioconcentration factor in the roots and enhanced total As uptake per plant.

CONCLUSIONS: Based on the results and multivariate analyses, both AMF species, particularly R. irregularis, enhanced safflower's As tolerance by retaining As in roots, improving phosphorus nutrition, and increasing antioxidant enzyme activity, showcasing their potential to enhance phytostabilization in safflower plants.},
}

*Mycorrhizae/physiology
*Carthamus tinctorius/microbiology/metabolism/drug effects
*Glomeromycota/physiology
*Arsenic/metabolism/toxicity
*Soil Pollutants/metabolism/toxicity
*Biodegradation, Environmental
Symbiosis
Plant Roots/microbiology/metabolism/growth & development
Chlorophyll/metabolism
Phosphorus/metabolism
Fungi

@article {pmid39653531,
year = {2024},
author = {Wang, W and Wang, FR and Guo, Y and Zhang, HB and Jiang, FF},
title = {[Characteristics of airway microbiome co-occurrence network in patients with type 2 and non-type 2 asthma].},
journal = {Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases},
volume = {47},
number = {12},
pages = {1121-1129},
doi = {10.3760/cma.j.cn112147-20241015-00611},
pmid = {39653531},
issn = {1001-0939},
mesh = {Humans ; *Asthma/microbiology/metabolism ; Female ; Male ; Middle Aged ; Adult ; Prospective Studies ; *Microbiota ; *Nitric Oxide/metabolism ; Sputum/microbiology ; Case-Control Studies ; Respiratory System/microbiology/metabolism ; Breath Tests ; },
abstract = {Objective: To study the characteristics of the airway microbiome co-occurrence network in patients with type 2 and non-type 2 asthma. Methods: In a prospective study based on a cohort of asthma patients, respiratory induced sputum samples were collected from 55 asthma patients [25 males and 30 females, with a median age of 47.7 years (age range 34.3-63.0 years)] admitted to the Department of Respiratory and Critical Care, Beijing Chaoyang Hospital, Capital Medical University and 12 healthy controls from the Physical Examination Centre of Beijing Chaoyang Hospital, Capital Medical University, from May 2021 to May 2022. According to the level of exhaled breath nitric oxide (FeNO), the asthma patients were divided into 22 cases in the high FeNO group (FeNO≥40 ppb, i.e., type 2 asthma group) and 33 cases in the low FeNO group (FeNO<40 ppb, i.e., non-type 2 asthma group). All induced sputum samples were subjected to second-generation macrogenomic sequencing and bioinformatic analyses of microbial community diversity, compositional characteristics, symbiotic network characteristics and metabolic function prediction. The Kruskal-Wallis rank sum test was used for between-group comparisons, and the linear discriminant analysis (LEfSe) method was used to compare the differences in flora composition between groups. The R language was used for microbial network analysis. In addition, PICRUSt was used to predict the metabolic-functional characteristics of the microbial communities. Results: The microbial communities in the healthy control group had a lower proportion of p_Firmicutes and p_Proteobacteria than asthma patients, 29% and 21%, respectively; 37% and 33% in the low FeNO group and 42% and 26% in the high FeNO group. The microbial network in the low FeNO group had 64 pairs of edges forming 16 communities, and about 75% of the nodes had eigenvector centrality values between 0 and 0.05, and 25% of the nodes had eigenvector centrality values between 0.10 and 0.45. There were four layers of κ-nucleosynthesis, and about 42% of the vertices were in the centre of the two layers. The microbial network of the high-FeNO group had 80 pairs of edges forming 18 clusters, and 81% of the nodes had eigenvector centrality values between 0 and 0.05, and 19% of the nodes had eigenvector centrality values between 0.10 and 0.35. The κ-nucleus decomposition had eight layers, and 21% of the vertices were located in the centre's two layers. The main functional differences between the low and high FeNO groups were shown in metabolic pathways (including sugar, lipid, amino acid, and energy metabolism), drug resistance, biofilm transport, signalling, intercellular communication, and cellular repair. Conclusions: Compared with non-type 2 asthmatics, type 2 asthmatics had a higher alpha diversity of respiratory microbiota, lower levels of microorganisms in the p_Proteobacteria, and a more aggregated microbial network. There was a significant difference in the predicted metabolic function of the two endotypes of asthmatics.},
}

Humans
*Asthma/microbiology/metabolism
Female
Male
Middle Aged
Adult
Prospective Studies
*Microbiota
*Nitric Oxide/metabolism
Sputum/microbiology
Case-Control Studies
Respiratory System/microbiology/metabolism
Breath Tests

@article {pmid39653482,
year = {2024},
author = {Newsham, KK and Foot, GW and Sands, CJ and Goodall-Copestake, WP},
title = {A cosmopolitan Serendipita forms mycothalli with sub-Antarctic leafy liverworts.},
journal = {Fungal biology},
volume = {128},
number = {8 Pt B},
pages = {2355-2364},
doi = {10.1016/j.funbio.2023.11.006},
pmid = {39653482},
issn = {1878-6146},
mesh = {*Hepatophyta/microbiology ; *Phylogeny ; *DNA, Fungal/genetics ; Sequence Analysis, DNA ; Antarctic Regions ; Symbiosis ; DNA, Ribosomal Spacer/genetics/chemistry ; DNA, Ribosomal/genetics/chemistry ; Ascomycota/genetics/classification/isolation & purification/cytology ; Molecular Sequence Data ; Microscopy ; },
abstract = {The occurrence of mycothalli, symbioses between liverworts and fungi, is poorly documented in sub-Antarctica, and biogeographical patterns in Serendipita, the main fungal genus forming the symbiosis, remain understudied. Here, 83 specimens of 16 leafy liverwort species were sampled from sub-Antarctic South Georgia and were examined for mycothalli. Microscopy was used to enumerate fungal structures in liverwort tissues, and sequencing of fungal ribosomal DNA was used to determine the taxonomic and biogeographical affinities of the fungi. Stained hyphal coils, a defining feature of the symbiosis, were found to be frequent (>40% of stem length colonised) in Barbilophozia hatcheri, Cephaloziella varians and Lophoziopsis excisa. A single species of Serendipita, based on a 3% cut-off for ITS2 region sequence divergence, was a frequent colonist of these liverworts. A further 18 basidiomycete and ascomycete taxa colonised other liverwort species. The presence of the Serendipita species was positively associated with the occurrence of stained hyphal coils in stem epidermal cells. Phylogenetic analyses, incorporating worldwide accessions from leafy liverwort-associated Serendipita, showed that the same species, which also occurs in Chile, mainland Europe and on Svalbard, is apparently the sole symbiont of sub- and maritime Antarctic leafy liverworts, and indicated much higher species richness of the genus outside Antarctica.},
}

*Hepatophyta/microbiology
*Phylogeny
*DNA, Fungal/genetics
Sequence Analysis, DNA
Antarctic Regions
Symbiosis
DNA, Ribosomal Spacer/genetics/chemistry
DNA, Ribosomal/genetics/chemistry
Ascomycota/genetics/classification/isolation & purification/cytology
Molecular Sequence Data
Microscopy

@article {pmid39653175,
year = {2024},
author = {Wei, Y and Xia, W and Qian, Y and Rong, C and Ye, M and Yujie, C and Kikuchi, J and Li, YY},
title = {Revealing microbial compatibility of partial nitritation/Anammox biofilm from sidestream to mainstream applications: Origins, dynamics, and interrelationships.},
journal = {Bioresource technology},
volume = {},
number = {},
pages = {131963},
doi = {10.1016/j.biortech.2024.131963},
pmid = {39653175},
issn = {1873-2976},
abstract = {Biofilms offer a solution to the challenge of low biomass retention faced in mainstream partial nitritation/Anammox (PN/A) applications. In this study, a one-stage PN/A reactor derived from initial granular sludge was successfully transformed into a biofilm system using shedding carriers. Environmental stressors, such as ammonium nitrogen concentration and organic matter, significantly affected the competitive dynamics and dominant species composition between Ca. Kuenenia and Ca. Brocadia. Under approximately 500 mg/L NH4[+]-N, Ca. Brocadia emerged as the dominant anammox bacteria species, but was subsequently replaced by Ca. Kuenenia in the presence of approximately 54 mg COD/L CH3COONa. Moreover, Chloroflexi species on the original biofilm exhibited an associated relationship with the growth of Ca. Kuenenia in new biofilm. The biofilm assembly and microbial community migration uniquely reveal the microbial niche dynamics. This study provides valuable insights for PN/A biofilm applications facing diverse challenges of environmental stresses in the transition from sidestream to mainstream.},
}

@article {pmid39653007,
year = {2024},
author = {Xia, Z and Xue, C and Liu, R and Hui, Q and Hu, B and Rennenberg, H},
title = {Lead accumulation and concomitant reactive oxygen species (ROS) scavenging in Robinia pseudoacacia are dependent on nitrogen nutrition.},
journal = {Plant physiology and biochemistry : PPB},
volume = {219},
number = {},
pages = {109388},
doi = {10.1016/j.plaphy.2024.109388},
pmid = {39653007},
issn = {1873-2690},
abstract = {Heavy metal pollution combined with nitrogen (N) limitation is a major factor preventing revegetation of contaminated land. Woody N2-fixing legumes are a natural choice for phytoremediation. However, the physiological responses of woody legumes to lead (Pb) with low N exposure are currently unknown. In the present study, a common Robinia cultivar from Northeast China, inoculated and non-inoculated with rhizobia, was exposed to -Pb or + Pb at moderate (norN) or low N application (lowN). Our results showed that without inoculation, independent of N application, Pb taken up by the roots was allocated to the shoot and inhibited photosynthesis and biomass production. In non-inoculated Robinia, Pb-mediated oxidative stress resulted in reduced H2O2 scavenging as indicated by increased ascorbate peroxidase (APX) activity in the leaves and proline contents in the roots, independent of N application. Combined lowN∗Pb exposure significantly increased malondialdehyde (MDA) contents in roots and leaves and enhanced APX and dehydroascorbate reductase activities in leaves compared to individual Pb exposure. Rhizobia inoculation raised the abundance of nodules and promoted Pb uptake by roots. Under Pb exposure, inoculation with rhizobia reduced MDA contents, increased proline contents in leaves and roots and enhanced activity of nitrate reductase in the leaves, independent of N application. Under Pb exposure, nitrogenase activity of inoculated Robinia under low- and norN application were similar indicating that enhanced of N2-fixation at lowN was counteracted by Pb exposure. These results show that inoculation of Robinia with rhizobia can alleviate Pb toxicity at combined lowN and Pb exposure by reducing oxidative stress.},
}