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ESP: PubMed Auto Bibliography 29 Oct 2024 at 01:56 Created:
Symbiosis
Symbiosis refers to an interaction between two or more different organisms living in close physical association, typically to the advantage of both. Symbiotic relationships were once thought to be exceptional situations. Recent studies, however, have shown that every multicellular eukaryote exists in a tight symbiotic relationship with billions of microbes. The associated microbial ecosystems are referred to as microbiome and the combination of a multicellular organism and its microbiota has been described as a holobiont. It seems "we are all lichens now."
Created with PubMed® Query: ( symbiosis[tiab] OR symbiotic[tiab] ) NOT pmcbook NOT ispreviousversion
Citations The Papers (from PubMed®)
RevDate: 2024-10-26
Ectomycorrhizal fungal communities associated with Crocanthemum and Lechea (Cistaceae) in subtropical Florida sandhill habitats.
Mycorrhiza [Epub ahead of print].
Cistaceae are shrubs, subshrubs and herbs that often occur in stressful, fire-prone or disturbed environments and form ectomycorrhizal (ECM) associations with symbiotic fungi. Although some Cistaceae are long-lived shrubs that grow to significant size, others are herbaceous annuals or short-lived plants. Thus, Cistaceae are atypical ECM hosts that are fundamentally different in their biology from trees that are the more typically studied ECM hosts. The Mediterranean region is the center of diversity for Cistaceae and the ectomycorrhizal fungi associated with Cistaceae hosts have primarily been studied in Europe, North Africa, and the Middle East. Mediterranean Cistaceae often host diverse communities of ECM fungi, but they also act as hosts for some ECM fungi that putatively show host-specificity or strong host preference for Cistaceae (including species of Delastria, Hebeloma, Terfezia, and Tirmania). The ECM associations of Cistaceae in North America, however, remain highly understudied. Here we use fungal DNA metabarcoding to document the ectomycorrhizal fungal communities associated with Crocanthemum and Lechea (Cistaceae) in open, fire-prone sandhill habitats in north Florida. At each site we also sampled nearby Pinus to determine whether small, herbaceous Cistaceae have specialized ECM fungi or whether they share their ECM fungal community with nearby pines. The ECM communities of Florida Cistaceae are dominated by Cenococcum (Ascomycota) and Russula (Basidiomycota) species but were also significantly associated with Delastria, an understudied genus of mostly truffle-like Pezizales (Ascomycota). Although many Cistaceae ECM fungi were shared with neighboring pines, the ECM communities with Cistaceae were nonetheless significantly different than those of pines.
Additional Links: PMID-39460782
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@article {pmid39460782,
year = {2024},
author = {Caiafa, MV and Grazziotti, PH and Karlsen-Ayala, E and Jusino, MA and Healy, R and Reynolds, NK and Whitten, WM and Smith, ME},
title = {Ectomycorrhizal fungal communities associated with Crocanthemum and Lechea (Cistaceae) in subtropical Florida sandhill habitats.},
journal = {Mycorrhiza},
volume = {},
number = {},
pages = {},
pmid = {39460782},
issn = {1432-1890},
support = {PVEX-88881170665/2018-01//Brazilian Federal Agency for Support and Evaluation of Graduate Education/ ; Hatch project 1001991 and McIntire-Stennis project 1011527//U.S. Department of Agriculture National Institute of Food and Agriculture/ ; Ordway-Swisher Jumpstart Funding//Institute for Food and Agricultural Sciences at University of Florida/ ; DEB-2106130//United States National Science Foundation/ ; },
abstract = {Cistaceae are shrubs, subshrubs and herbs that often occur in stressful, fire-prone or disturbed environments and form ectomycorrhizal (ECM) associations with symbiotic fungi. Although some Cistaceae are long-lived shrubs that grow to significant size, others are herbaceous annuals or short-lived plants. Thus, Cistaceae are atypical ECM hosts that are fundamentally different in their biology from trees that are the more typically studied ECM hosts. The Mediterranean region is the center of diversity for Cistaceae and the ectomycorrhizal fungi associated with Cistaceae hosts have primarily been studied in Europe, North Africa, and the Middle East. Mediterranean Cistaceae often host diverse communities of ECM fungi, but they also act as hosts for some ECM fungi that putatively show host-specificity or strong host preference for Cistaceae (including species of Delastria, Hebeloma, Terfezia, and Tirmania). The ECM associations of Cistaceae in North America, however, remain highly understudied. Here we use fungal DNA metabarcoding to document the ectomycorrhizal fungal communities associated with Crocanthemum and Lechea (Cistaceae) in open, fire-prone sandhill habitats in north Florida. At each site we also sampled nearby Pinus to determine whether small, herbaceous Cistaceae have specialized ECM fungi or whether they share their ECM fungal community with nearby pines. The ECM communities of Florida Cistaceae are dominated by Cenococcum (Ascomycota) and Russula (Basidiomycota) species but were also significantly associated with Delastria, an understudied genus of mostly truffle-like Pezizales (Ascomycota). Although many Cistaceae ECM fungi were shared with neighboring pines, the ECM communities with Cistaceae were nonetheless significantly different than those of pines.},
}
RevDate: 2024-10-26
Understanding the crucial role of phosphate and iron availability in regulating root nodule symbiosis.
Plant & cell physiology pii:7842828 [Epub ahead of print].
The symbiosis between legumes and nitrogen-fixing bacteria (rhizobia) is instrumental in sustaining the nitrogen cycle and providing fixed nitrogen to the food chain. Both partners must maintain an efficient nutrient exchange to ensure a successful symbiosis. This mini-review highlights the intricate phosphate and iron uptake and homeostasis processes taking place in legumes during their interactions with rhizobia. The coordination of transport and homeostasis of these nutrients in host plants and rhizobia ensures an efficient nitrogen fixation process and nutrient use. We discuss the genetic machinery controlling the uptake and homeostasis of these nutrients in the absence of rhizobia and in symbiotic conditions with this soil bacteria. We also highlight the genetic impact of the availability of phosphate and iron to coordinate the activation of the genetic programs that allow legumes to engage in symbiosis with rhizobia. Finally, we discuss how the transcription factor Phosphate Starvation Response (PHR) might be a crucial genetic element to integrate the nitrogen, iron, and phosphate plant's needs while interacting with rhizobia. Understanding the coordination of the iron and phosphate uptake and homeostasis can lead us to better harness the ecological benefits of the legume-rhizobia symbiosis, even under adverse environmental conditions.
Additional Links: PMID-39460549
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@article {pmid39460549,
year = {2024},
author = {Isidra-Arellano, MC and Valdés-López, O},
title = {Understanding the crucial role of phosphate and iron availability in regulating root nodule symbiosis.},
journal = {Plant & cell physiology},
volume = {},
number = {},
pages = {},
doi = {10.1093/pcp/pcae128},
pmid = {39460549},
issn = {1471-9053},
support = {IN200523//Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica (PAPIIT-UNAM)/ ; IN200523//Unam/ ; //Royal Society/ ; },
abstract = {The symbiosis between legumes and nitrogen-fixing bacteria (rhizobia) is instrumental in sustaining the nitrogen cycle and providing fixed nitrogen to the food chain. Both partners must maintain an efficient nutrient exchange to ensure a successful symbiosis. This mini-review highlights the intricate phosphate and iron uptake and homeostasis processes taking place in legumes during their interactions with rhizobia. The coordination of transport and homeostasis of these nutrients in host plants and rhizobia ensures an efficient nitrogen fixation process and nutrient use. We discuss the genetic machinery controlling the uptake and homeostasis of these nutrients in the absence of rhizobia and in symbiotic conditions with this soil bacteria. We also highlight the genetic impact of the availability of phosphate and iron to coordinate the activation of the genetic programs that allow legumes to engage in symbiosis with rhizobia. Finally, we discuss how the transcription factor Phosphate Starvation Response (PHR) might be a crucial genetic element to integrate the nitrogen, iron, and phosphate plant's needs while interacting with rhizobia. Understanding the coordination of the iron and phosphate uptake and homeostasis can lead us to better harness the ecological benefits of the legume-rhizobia symbiosis, even under adverse environmental conditions.},
}
RevDate: 2024-10-28
Current Trends and Technological Advancements in the Use of Oxalate-Degrading Bacteria as Starters in Fermented Foods-A Review.
Life (Basel, Switzerland), 14(10):.
Nephrolithiasis is a medical condition characterized by the existence or development of calculi, commonly referred to as stones within the renal system, and poses significant health challenges. Calcium phosphate and calcium oxalate are the predominant constituents of renal calculi and are introduced into the human body primarily via dietary sources. The presence of oxalates can become particularly problematic when the delicate balance of the normal flora residing within the gastrointestinal tract is disrupted. Within the human gut, species of Oxalobacter, Lactobacillus, and Bifidobacterium coexist in a symbiotic relationship. They play a pivotal role in mitigating the risk of stone formation by modulating certain biochemical pathways and producing specific enzymes that can facilitate the breakdown and degradation of oxalate salts. The probiotic potential exhibited by these bacteria is noteworthy, as it underscores their possible utility in the prevention of nephrolithiasis. Investigating the mechanisms by which these beneficial microorganisms exert their effects could lead to novel therapeutic strategies aimed at reducing the incidence of kidney stones. The implications of utilizing probiotics as a preventive measure against kidney stone formation represent an intriguing frontier in both nephrology and microbiome research, meriting further investigation to unlock their full potential.
Additional Links: PMID-39459637
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@article {pmid39459637,
year = {2024},
author = {Al-Kabe, SH and Niamah, AK},
title = {Current Trends and Technological Advancements in the Use of Oxalate-Degrading Bacteria as Starters in Fermented Foods-A Review.},
journal = {Life (Basel, Switzerland)},
volume = {14},
number = {10},
pages = {},
pmid = {39459637},
issn = {2075-1729},
abstract = {Nephrolithiasis is a medical condition characterized by the existence or development of calculi, commonly referred to as stones within the renal system, and poses significant health challenges. Calcium phosphate and calcium oxalate are the predominant constituents of renal calculi and are introduced into the human body primarily via dietary sources. The presence of oxalates can become particularly problematic when the delicate balance of the normal flora residing within the gastrointestinal tract is disrupted. Within the human gut, species of Oxalobacter, Lactobacillus, and Bifidobacterium coexist in a symbiotic relationship. They play a pivotal role in mitigating the risk of stone formation by modulating certain biochemical pathways and producing specific enzymes that can facilitate the breakdown and degradation of oxalate salts. The probiotic potential exhibited by these bacteria is noteworthy, as it underscores their possible utility in the prevention of nephrolithiasis. Investigating the mechanisms by which these beneficial microorganisms exert their effects could lead to novel therapeutic strategies aimed at reducing the incidence of kidney stones. The implications of utilizing probiotics as a preventive measure against kidney stone formation represent an intriguing frontier in both nephrology and microbiome research, meriting further investigation to unlock their full potential.},
}
RevDate: 2024-10-28
Alleviation of NaCl Stress on Growth and Biochemical Traits of Cenchrus ciliaris L. via Arbuscular Mycorrhizal Fungi Symbiosis.
Life (Basel, Switzerland), 14(10):.
Soil salinization, especially in arid and semi-arid regions, is one of the major abiotic stresses that affect plant growth. To mediate and boost plant tolerance against this abiotic stress, arbuscular mycorrhizal fungi (AMF) symbiosis is commonly thought to be an effective tool. So, the main purpose of this study was to estimate the role of AMF (applied as a consortium of Claroideoglomus etunicatum, Funneliformis mosseae, Rhizophagus fasciculatum, and R. intraradices species) symbiosis in mitigating deleterious salt stress effects on the growth parameters (shoot length (SL), root length (RL), shoot dry weight (SDW), root dry weight (RDW), root surface area (RSA), total root length (TRL), root volume (RV), root diameter (RD), number of nodes and leaves) of Cenchrus ciliaris L. plants through improved accumulations of photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll), proline and phenolic compounds. The results of this experiment revealed that the roots of C. ciliaris plants were colonized by AMF under all the applied salinity levels (0, 75, 150, 225, and 300 mM NaCl). However, the rate of colonization was negatively affected by increasing salinity as depicted by the varied colonization structures (mycelium, vesicles, arbuscules and spores) which were highest under non-saline conditions. This association of AMF induced an increase in the growth parameters of the plant which were reduced by salinity stress. The improved shoot/root indices are likely due to enhanced photosynthetic activities as the AMF-treated plants showed increased accumulation of pigments (chlorophyll a, chlorophyll b and total chlorophyll), under saline as well as non-saline conditions, compared to non-AMF (N-AMF) plants. Furthermore, the AMF-treated plants also exhibited enhanced accumulation of proline and phenolic compounds. These accumulated metabolites act as protective measures under salinity stress, hence explaining the improved photosynthetic and growth parameters of the plants. These results suggest that AMF could be a good tool for the restoration of salt-affected habitats. However, more research is needed to check the true efficacy of different AMF inoculants under field conditions.
Additional Links: PMID-39459576
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@article {pmid39459576,
year = {2024},
author = {Malik, JA and Alqarawi, AA and Alotaibi, F and Habib, MM and Sorrori, SN and Almutairi, MBR and Dar, BA},
title = {Alleviation of NaCl Stress on Growth and Biochemical Traits of Cenchrus ciliaris L. via Arbuscular Mycorrhizal Fungi Symbiosis.},
journal = {Life (Basel, Switzerland)},
volume = {14},
number = {10},
pages = {},
pmid = {39459576},
issn = {2075-1729},
support = {RSPD2024R889//King Saud University, Riyadh, Saudi Arabia/ ; },
abstract = {Soil salinization, especially in arid and semi-arid regions, is one of the major abiotic stresses that affect plant growth. To mediate and boost plant tolerance against this abiotic stress, arbuscular mycorrhizal fungi (AMF) symbiosis is commonly thought to be an effective tool. So, the main purpose of this study was to estimate the role of AMF (applied as a consortium of Claroideoglomus etunicatum, Funneliformis mosseae, Rhizophagus fasciculatum, and R. intraradices species) symbiosis in mitigating deleterious salt stress effects on the growth parameters (shoot length (SL), root length (RL), shoot dry weight (SDW), root dry weight (RDW), root surface area (RSA), total root length (TRL), root volume (RV), root diameter (RD), number of nodes and leaves) of Cenchrus ciliaris L. plants through improved accumulations of photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll), proline and phenolic compounds. The results of this experiment revealed that the roots of C. ciliaris plants were colonized by AMF under all the applied salinity levels (0, 75, 150, 225, and 300 mM NaCl). However, the rate of colonization was negatively affected by increasing salinity as depicted by the varied colonization structures (mycelium, vesicles, arbuscules and spores) which were highest under non-saline conditions. This association of AMF induced an increase in the growth parameters of the plant which were reduced by salinity stress. The improved shoot/root indices are likely due to enhanced photosynthetic activities as the AMF-treated plants showed increased accumulation of pigments (chlorophyll a, chlorophyll b and total chlorophyll), under saline as well as non-saline conditions, compared to non-AMF (N-AMF) plants. Furthermore, the AMF-treated plants also exhibited enhanced accumulation of proline and phenolic compounds. These accumulated metabolites act as protective measures under salinity stress, hence explaining the improved photosynthetic and growth parameters of the plants. These results suggest that AMF could be a good tool for the restoration of salt-affected habitats. However, more research is needed to check the true efficacy of different AMF inoculants under field conditions.},
}
RevDate: 2024-10-28
The Gut Microbiota Impacts Gastrointestinal Cancers through Obesity, Diabetes, and Chronic Inflammation.
Life (Basel, Switzerland), 14(10):.
The gut microbiota's pivotal role in human health is increasingly evident, particularly in chronic conditions like obesity, diabetes, and inflammatory diseases. This intricate symbiotic relationship influences metabolic balance and immune responses. Notably, gut microbial dysbiosis is linked to obesity's metabolic disruption and chronic low-grade inflammation. Similarly, in diabetes, the microbiota's impact on insulin resistance and glucose metabolism is becoming evident. Chronic inflammation, a common denominator in these conditions, is also a recognized precursor to carcinogenesis. This intersection prompts a compelling question: does the gut microbiota's influence extend to gastrointestinal cancers like colorectal and pancreatic cancer? These malignancies are closely intertwined with inflammation and metabolic dysregulation. Exploring whether the microbial signatures associated with chronic conditions overlap with precancerous or cancerous states offers new perspectives. This article reviews emerging evidence on the interplay between the gut microbiota, chronic conditions, and gastrointestinal cancers. By elucidating these connections, we aim to uncover potential avenues for innovative diagnostic, preventative, and therapeutic strategies in colorectal and pancreatic cancer management.
Additional Links: PMID-39459519
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@article {pmid39459519,
year = {2024},
author = {Rumyantsev, KA and Polyakova, VV and Sorokina, IV and Feoktistova, PS and Khatkov, IE and Bodunova, NA and Zhukova, LG},
title = {The Gut Microbiota Impacts Gastrointestinal Cancers through Obesity, Diabetes, and Chronic Inflammation.},
journal = {Life (Basel, Switzerland)},
volume = {14},
number = {10},
pages = {},
pmid = {39459519},
issn = {2075-1729},
support = {0309-2/22.//This work was supported by the Moscow Government under/ ; },
abstract = {The gut microbiota's pivotal role in human health is increasingly evident, particularly in chronic conditions like obesity, diabetes, and inflammatory diseases. This intricate symbiotic relationship influences metabolic balance and immune responses. Notably, gut microbial dysbiosis is linked to obesity's metabolic disruption and chronic low-grade inflammation. Similarly, in diabetes, the microbiota's impact on insulin resistance and glucose metabolism is becoming evident. Chronic inflammation, a common denominator in these conditions, is also a recognized precursor to carcinogenesis. This intersection prompts a compelling question: does the gut microbiota's influence extend to gastrointestinal cancers like colorectal and pancreatic cancer? These malignancies are closely intertwined with inflammation and metabolic dysregulation. Exploring whether the microbial signatures associated with chronic conditions overlap with precancerous or cancerous states offers new perspectives. This article reviews emerging evidence on the interplay between the gut microbiota, chronic conditions, and gastrointestinal cancers. By elucidating these connections, we aim to uncover potential avenues for innovative diagnostic, preventative, and therapeutic strategies in colorectal and pancreatic cancer management.},
}
RevDate: 2024-10-28
CmpDate: 2024-10-26
Identification and Assessment of Secondary Metabolites from Three Fungal Endophytes of Solanum mauritianum Against Public Health Pathogens.
Molecules (Basel, Switzerland), 29(20):.
Fungal endophytes, symbiotic microorganisms residing within plants, are renowned for producing bioactive secondary metabolites with diverse beneficial properties. We investigated the antimicrobial potential of fungal endophytes isolated from Solanum mauritianum, an invasive weed, against clinically significant bacterial pathogens. Selected fungal endophytes (Penicillium chrysogenum, Fusarium sp., and Paracamarosporium leucadendri) were isolated from the plant's leaves and fruits. Their crude extracts were tested against various referenced strains, such as Mycobacterium species (M. smegmatis ATCC 607 and M. bovis ATCC 27290), Staphylococcus aureus ATCC 6571, Bacillus subtilis ATCC 11774, Klebsiella species (K. pneumoniae ATCC 10031 and K. oxytoca ATCC 8724), Escherichia coli ATCC 10536, and Pseudomonas aeruginosa ATCC 10145, using the Kirby-Bauer disk diffusion method. Resazurin Microtiter Assay was used for the determination of the minimum inhibitory concentration. The chemical nature of the secondary metabolites in the crude extracts produced by fungal endophytes was evaluated using high-resolution liquid chromatography-mass spectrometry (LC-MS) using water and acetonitrile gradient. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS) was employed for untargeted metabolomics. LC-QTOF-MS/MS identified 63 bioactive compounds across the three endophytes. P. chrysogenum had the highest activity against S. aureus and M. smegmatis (1.15 mg/mL and 0.02 mg/mL, respectively), while P. leucadendri demonstrated moderate activity against M. smegmatis (2.91 mg/mL) and E. coli (1.16 mg/mL). Fusarium sp. exhibited the broadest spectrum of antibacterial activity, with MIC values ranging from 0.03 mg/mL (B. subtilis) to 10 mg/mL (M. smegmatis). P. leucadendri produced 29 metabolites, Fusarium sp. had 23 identified metabolites, and a total of 11 metabolites were identified from P. chrysogenum. The fruits of the plant, accounting for 60%, appeared to be the most abundant in the endophyte diversity when compared to the stems and leaves. This study highlights the potential of fungal endophytes from S. mauritianum as a source of novel bioactive compounds, particularly against multidrug-resistant pathogens, contributing to the ongoing efforts to combat antimicrobial resistance.
Additional Links: PMID-39459292
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@article {pmid39459292,
year = {2024},
author = {Ogofure, AG and Pelo, SP and Green, E},
title = {Identification and Assessment of Secondary Metabolites from Three Fungal Endophytes of Solanum mauritianum Against Public Health Pathogens.},
journal = {Molecules (Basel, Switzerland)},
volume = {29},
number = {20},
pages = {},
pmid = {39459292},
issn = {1420-3049},
mesh = {*Solanum/microbiology/chemistry ; *Endophytes/metabolism ; *Microbial Sensitivity Tests ; Secondary Metabolism ; Anti-Bacterial Agents/pharmacology/chemistry ; Fungi/metabolism ; Metabolomics/methods ; Bacteria/metabolism ; Plant Leaves/microbiology/chemistry ; Tandem Mass Spectrometry ; Fusarium/metabolism ; },
abstract = {Fungal endophytes, symbiotic microorganisms residing within plants, are renowned for producing bioactive secondary metabolites with diverse beneficial properties. We investigated the antimicrobial potential of fungal endophytes isolated from Solanum mauritianum, an invasive weed, against clinically significant bacterial pathogens. Selected fungal endophytes (Penicillium chrysogenum, Fusarium sp., and Paracamarosporium leucadendri) were isolated from the plant's leaves and fruits. Their crude extracts were tested against various referenced strains, such as Mycobacterium species (M. smegmatis ATCC 607 and M. bovis ATCC 27290), Staphylococcus aureus ATCC 6571, Bacillus subtilis ATCC 11774, Klebsiella species (K. pneumoniae ATCC 10031 and K. oxytoca ATCC 8724), Escherichia coli ATCC 10536, and Pseudomonas aeruginosa ATCC 10145, using the Kirby-Bauer disk diffusion method. Resazurin Microtiter Assay was used for the determination of the minimum inhibitory concentration. The chemical nature of the secondary metabolites in the crude extracts produced by fungal endophytes was evaluated using high-resolution liquid chromatography-mass spectrometry (LC-MS) using water and acetonitrile gradient. Liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS/MS) was employed for untargeted metabolomics. LC-QTOF-MS/MS identified 63 bioactive compounds across the three endophytes. P. chrysogenum had the highest activity against S. aureus and M. smegmatis (1.15 mg/mL and 0.02 mg/mL, respectively), while P. leucadendri demonstrated moderate activity against M. smegmatis (2.91 mg/mL) and E. coli (1.16 mg/mL). Fusarium sp. exhibited the broadest spectrum of antibacterial activity, with MIC values ranging from 0.03 mg/mL (B. subtilis) to 10 mg/mL (M. smegmatis). P. leucadendri produced 29 metabolites, Fusarium sp. had 23 identified metabolites, and a total of 11 metabolites were identified from P. chrysogenum. The fruits of the plant, accounting for 60%, appeared to be the most abundant in the endophyte diversity when compared to the stems and leaves. This study highlights the potential of fungal endophytes from S. mauritianum as a source of novel bioactive compounds, particularly against multidrug-resistant pathogens, contributing to the ongoing efforts to combat antimicrobial resistance.},
}
MeSH Terms:
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hide MeSH Terms
*Solanum/microbiology/chemistry
*Endophytes/metabolism
*Microbial Sensitivity Tests
Secondary Metabolism
Anti-Bacterial Agents/pharmacology/chemistry
Fungi/metabolism
Metabolomics/methods
Bacteria/metabolism
Plant Leaves/microbiology/chemistry
Tandem Mass Spectrometry
Fusarium/metabolism
RevDate: 2024-10-26
Dynamics of Hydrogen Peroxide Accumulation During Tip Growth of Infection Thread in Nodules and Cell Differentiation in Pea (Pisum sativum L.) Symbiotic Nodules.
Plants (Basel, Switzerland), 13(20):.
Hydrogen peroxide (H2O2) in plants is produced in relatively large amounts and plays a universal role in plant defense and physiological responses, including the regulation of growth and development. In the Rhizobium-legume symbiosis, hydrogen peroxide plays an important signaling role throughout the development of this interaction. In the functioning nodule, H2O2 has been shown to be involved in bacterial differentiation into the symbiotic form and in nodule senescence. In this study, the pattern of H2O2 accumulation in pea (Pisum sativum L.) wild-type and mutant nodules blocked at different stages of the infection process was analyzed using a cytochemical reaction with cerium chloride. The observed dynamics of H2O2 deposition in the infection thread walls indicated that the distribution of H2O2 was apparently related to the stiffness of the infection thread wall. The dynamics of H2O2 accumulation was traced, and its patterns in different nodule zones were determined in order to investigate the relationship of H2O2 localization and distribution with the stages of symbiotic nodule development in P. sativum. The patterns of H2O2 localization in different zones of the indeterminate nodule have been partially confirmed by comparative analysis on mutant genotypes.
Additional Links: PMID-39458872
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@article {pmid39458872,
year = {2024},
author = {Tsyganova, AV and Gorshkov, AP and Vorobiev, MG and Tikhonovich, IA and Brewin, NJ and Tsyganov, VE},
title = {Dynamics of Hydrogen Peroxide Accumulation During Tip Growth of Infection Thread in Nodules and Cell Differentiation in Pea (Pisum sativum L.) Symbiotic Nodules.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {20},
pages = {},
pmid = {39458872},
issn = {2223-7747},
support = {23-16-00090//Russian Science Foundation/ ; },
abstract = {Hydrogen peroxide (H2O2) in plants is produced in relatively large amounts and plays a universal role in plant defense and physiological responses, including the regulation of growth and development. In the Rhizobium-legume symbiosis, hydrogen peroxide plays an important signaling role throughout the development of this interaction. In the functioning nodule, H2O2 has been shown to be involved in bacterial differentiation into the symbiotic form and in nodule senescence. In this study, the pattern of H2O2 accumulation in pea (Pisum sativum L.) wild-type and mutant nodules blocked at different stages of the infection process was analyzed using a cytochemical reaction with cerium chloride. The observed dynamics of H2O2 deposition in the infection thread walls indicated that the distribution of H2O2 was apparently related to the stiffness of the infection thread wall. The dynamics of H2O2 accumulation was traced, and its patterns in different nodule zones were determined in order to investigate the relationship of H2O2 localization and distribution with the stages of symbiotic nodule development in P. sativum. The patterns of H2O2 localization in different zones of the indeterminate nodule have been partially confirmed by comparative analysis on mutant genotypes.},
}
RevDate: 2024-10-26
G-Quadruplex Structures as Epigenetic Regulatory Elements in Priming of Defense Genes upon Short-Term Trichoderma atroviride Inoculation in Maize.
Plants (Basel, Switzerland), 13(20):.
Symbiosis establishment between Trichoderma atroviride and plant roots triggers the priming of defense responses, among other effects. Currently, there is no clear evidence regarding the molecular mechanisms that allow the plant to remain alert to future stimulus, either by pathogen attack or any other abiotic stress. Epigenetic modifications have emerged as a strategy to explain the increased defense response of plants in a priming state conferred by Trichoderma. Recently, various non-canonical structures of nucleic acids, especially G-quadruplex structures (G-quadruplexes or G4s), have been identified as potential targets during the establishment or maintenance of plant signals. In the present study, we developed a screening test for the identification of putative G4-forming sequences (PQSs) in previously identified Z. mays priming genes. Bioinformatic analysis revealed the presence of PQSs in the promoter region of five essential genes playing a critical role in priming in maize. Biophysical and spectroscopy studies showed the formation of G4s by these PQSs in vitro, and ChIP assays demonstrate their formation in vivo. Therefore, G4 formation could play a role as an epigenetic regulatory mechanism involved in the long-lasting primed state in maize plants.
Additional Links: PMID-39458870
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@article {pmid39458870,
year = {2024},
author = {Agostini, RB and Piga, EJ and Bayón, C and Binolfi, A and Armas, P and Campos-Bermudez, VA and Rius, SP},
title = {G-Quadruplex Structures as Epigenetic Regulatory Elements in Priming of Defense Genes upon Short-Term Trichoderma atroviride Inoculation in Maize.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {20},
pages = {},
pmid = {39458870},
issn = {2223-7747},
support = {PICT 2018-2034//Agencia Nacional de Promoción de la Investigación, el Desarrollo Tecnológico y la Innovación/ ; },
abstract = {Symbiosis establishment between Trichoderma atroviride and plant roots triggers the priming of defense responses, among other effects. Currently, there is no clear evidence regarding the molecular mechanisms that allow the plant to remain alert to future stimulus, either by pathogen attack or any other abiotic stress. Epigenetic modifications have emerged as a strategy to explain the increased defense response of plants in a priming state conferred by Trichoderma. Recently, various non-canonical structures of nucleic acids, especially G-quadruplex structures (G-quadruplexes or G4s), have been identified as potential targets during the establishment or maintenance of plant signals. In the present study, we developed a screening test for the identification of putative G4-forming sequences (PQSs) in previously identified Z. mays priming genes. Bioinformatic analysis revealed the presence of PQSs in the promoter region of five essential genes playing a critical role in priming in maize. Biophysical and spectroscopy studies showed the formation of G4s by these PQSs in vitro, and ChIP assays demonstrate their formation in vivo. Therefore, G4 formation could play a role as an epigenetic regulatory mechanism involved in the long-lasting primed state in maize plants.},
}
RevDate: 2024-10-26
Cyclic Isothiocyanate Goitrin Impairs Lotus japonicus Nodulation, Affects the Proteomes of Nodules and Free Mesorhizobium loti, and Induces the Formation of Caffeic Acid Derivatives in Bacterial Cultures.
Plants (Basel, Switzerland), 13(20):.
The continuous release of glucosinolates into the soil by Brassicaceae root exudation is a prerequisite to maintaining toxic levels of breakdown products such as isothiocyanates (ITCs). ITCs influence plant and microbial diversity in ecosystems, while fungi and Rhizobiaceae are particularly injured. Studies explaining the molecular mechanisms of the negative effects are presently limited. Therefore, we investigated the early effects of cyclic ITC goitrin on proteomes of the host and symbiotic Mesorhizobium loti in the nodules of Lotus japonicus and of free-living bacteria. In the nodules, many host proteins had a higher abundance, among them, peroxidases and pathogenesis-related PR-10 proteins functioning in the abscisic-acid-activated signaling pathway. In the microsymbiont, transporter proteins as a prominent group are enhanced; some proteins involved in N-fixation decreased. The proteomes give a report about the loss of immunity suppression resulting in the termination of symbiosis, which initiates nodule senescence. Free-living M. loti are severely damaged, indicated, i.a., by a decrease in transporter proteins, the assumed candidates for goitrin protein complex formation, and high proteolysis. The production of chicoric acid by the accompanying bacteria is inhibitory for M. loti but connected to goitrin elimination, as confirmed by mass spectrometric (MS) analysis. In summary, the nodulation process is severely affected by goitrin, causing nodule dysfunction and failed nodule development. N deficiency conditions leads to yellowish leaves and leaf abscission.
Additional Links: PMID-39458844
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@article {pmid39458844,
year = {2024},
author = {Jeong, S and Schütz, V and Demir, F and Preusche, M and Huesgen, P and Bigler, L and Kovacic, F and Gutbrod, K and Dörmann, P and Schulz, M},
title = {Cyclic Isothiocyanate Goitrin Impairs Lotus japonicus Nodulation, Affects the Proteomes of Nodules and Free Mesorhizobium loti, and Induces the Formation of Caffeic Acid Derivatives in Bacterial Cultures.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {20},
pages = {},
pmid = {39458844},
issn = {2223-7747},
abstract = {The continuous release of glucosinolates into the soil by Brassicaceae root exudation is a prerequisite to maintaining toxic levels of breakdown products such as isothiocyanates (ITCs). ITCs influence plant and microbial diversity in ecosystems, while fungi and Rhizobiaceae are particularly injured. Studies explaining the molecular mechanisms of the negative effects are presently limited. Therefore, we investigated the early effects of cyclic ITC goitrin on proteomes of the host and symbiotic Mesorhizobium loti in the nodules of Lotus japonicus and of free-living bacteria. In the nodules, many host proteins had a higher abundance, among them, peroxidases and pathogenesis-related PR-10 proteins functioning in the abscisic-acid-activated signaling pathway. In the microsymbiont, transporter proteins as a prominent group are enhanced; some proteins involved in N-fixation decreased. The proteomes give a report about the loss of immunity suppression resulting in the termination of symbiosis, which initiates nodule senescence. Free-living M. loti are severely damaged, indicated, i.a., by a decrease in transporter proteins, the assumed candidates for goitrin protein complex formation, and high proteolysis. The production of chicoric acid by the accompanying bacteria is inhibitory for M. loti but connected to goitrin elimination, as confirmed by mass spectrometric (MS) analysis. In summary, the nodulation process is severely affected by goitrin, causing nodule dysfunction and failed nodule development. N deficiency conditions leads to yellowish leaves and leaf abscission.},
}
RevDate: 2024-10-26
Ionizing Radiation Dose Differentially Affects the Host-Microbe Relationship over Time.
Microorganisms, 12(10):.
Microorganisms that colonize in or on a host play significant roles in regulating the host's immunological fitness and bioenergy production, thus controlling the host's stress responses. Radiation elicits a pro-inflammatory and bioenergy-expensive state, which could influence the gut microbial compositions and, therefore, the host-microbe bidirectional relationship. To test this hypothesis, young adult mice were exposed to total body irradiation (TBI) at doses of 9.5 Gy and 11 Gy, respectively. The irradiated mice were euthanized on days 1, 3, and 9 post TBI, and their descending colon contents (DCCs) were collected. The 16S ribosomal RNAs from the DCCs were screened to find the differentially enriched bacterial taxa due to TBI. Subsequently, these data were analyzed to identify the metagenome-specific biofunctions. The bacterial community of the DCCs showed increased levels of diversity as time progressed following TBI. The abundance profile was the most divergent at day 9 post 11 Gy TBI. For instance, an anti-inflammatory and energy-harvesting bacterium, namely, Firmicutes, became highly abundant and co-expressed in the DCC with pro-inflammatory Deferribacteres at day 9 post 11 Gy TBI. A systems evaluation found a diverging trend in the regulation profiles of the functional networks that were linked to the bacteria and metabolites of the DCCs, respectively. Additionally, the network clusters associated with lipid metabolism and bioenergy synthesis were found to be activated in the DCC bacteria but inhibited in the metabolite space at day 9 post 11 Gy. Taking these results together, the present analysis indicated a disrupted mouse-bacteria symbiotic relationship as time progressed after lethal irradiation. This information can help develop precise interventions to ameliorate the symptoms triggered by TBI.
Additional Links: PMID-39458305
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@article {pmid39458305,
year = {2024},
author = {Chakraborty, N and Hoke, A and Campbell, R and Holmes-Hampton, G and Kumar, VP and Moyler, C and Gautam, A and Hammamieh, R and Ghosh, SP},
title = {Ionizing Radiation Dose Differentially Affects the Host-Microbe Relationship over Time.},
journal = {Microorganisms},
volume = {12},
number = {10},
pages = {},
pmid = {39458305},
issn = {2076-2607},
support = {DM178020 and RAB23338//JPC-7 project and the AFRRI Intramural funding/ ; },
abstract = {Microorganisms that colonize in or on a host play significant roles in regulating the host's immunological fitness and bioenergy production, thus controlling the host's stress responses. Radiation elicits a pro-inflammatory and bioenergy-expensive state, which could influence the gut microbial compositions and, therefore, the host-microbe bidirectional relationship. To test this hypothesis, young adult mice were exposed to total body irradiation (TBI) at doses of 9.5 Gy and 11 Gy, respectively. The irradiated mice were euthanized on days 1, 3, and 9 post TBI, and their descending colon contents (DCCs) were collected. The 16S ribosomal RNAs from the DCCs were screened to find the differentially enriched bacterial taxa due to TBI. Subsequently, these data were analyzed to identify the metagenome-specific biofunctions. The bacterial community of the DCCs showed increased levels of diversity as time progressed following TBI. The abundance profile was the most divergent at day 9 post 11 Gy TBI. For instance, an anti-inflammatory and energy-harvesting bacterium, namely, Firmicutes, became highly abundant and co-expressed in the DCC with pro-inflammatory Deferribacteres at day 9 post 11 Gy TBI. A systems evaluation found a diverging trend in the regulation profiles of the functional networks that were linked to the bacteria and metabolites of the DCCs, respectively. Additionally, the network clusters associated with lipid metabolism and bioenergy synthesis were found to be activated in the DCC bacteria but inhibited in the metabolite space at day 9 post 11 Gy. Taking these results together, the present analysis indicated a disrupted mouse-bacteria symbiotic relationship as time progressed after lethal irradiation. This information can help develop precise interventions to ameliorate the symptoms triggered by TBI.},
}
RevDate: 2024-10-26
The Impact of AI Usage on University Students' Willingness for Autonomous Learning.
Behavioral sciences (Basel, Switzerland), 14(10): pii:bs14100956.
As artificial intelligence (AI) technology becomes increasingly integrated into education, understanding the theoretical mechanisms that drive university students to adopt new learning behaviors through these tools is essential. This study extends the Expectation-Confirmation Model (ECM) by incorporating both cognitive and affective variables to examine students' current AI usage and their future expectations. The model includes intrinsic and extrinsic motivations, focusing on three key factors: positive emotions, digital efficacy, and willingness for autonomous learning. A survey of 721 valid responses revealed that positive emotions, digital efficacy, and satisfaction significantly influence continued AI usage, with positive emotions being particularly critical. Digital efficacy and perceived usefulness also impact satisfaction, but long-term usage intentions are more effectively driven by positive emotions. Furthermore, digital efficacy strongly affects the willingness for autonomous learning. Therefore, higher education institutions should promote AI technology, enhance students' expectation-confirmation levels, and emphasize positive emotional experiences during AI use. Adopting a "human-machine symbiosis" model can foster active learning, personalized learning pathways, and the development of students' digital efficacy and innovation capabilities.
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@article {pmid39457827,
year = {2024},
author = {Wang, L and Li, W},
title = {The Impact of AI Usage on University Students' Willingness for Autonomous Learning.},
journal = {Behavioral sciences (Basel, Switzerland)},
volume = {14},
number = {10},
pages = {},
doi = {10.3390/bs14100956},
pmid = {39457827},
issn = {2076-328X},
support = {BIA240122//National Social Science Foundation/ ; },
abstract = {As artificial intelligence (AI) technology becomes increasingly integrated into education, understanding the theoretical mechanisms that drive university students to adopt new learning behaviors through these tools is essential. This study extends the Expectation-Confirmation Model (ECM) by incorporating both cognitive and affective variables to examine students' current AI usage and their future expectations. The model includes intrinsic and extrinsic motivations, focusing on three key factors: positive emotions, digital efficacy, and willingness for autonomous learning. A survey of 721 valid responses revealed that positive emotions, digital efficacy, and satisfaction significantly influence continued AI usage, with positive emotions being particularly critical. Digital efficacy and perceived usefulness also impact satisfaction, but long-term usage intentions are more effectively driven by positive emotions. Furthermore, digital efficacy strongly affects the willingness for autonomous learning. Therefore, higher education institutions should promote AI technology, enhance students' expectation-confirmation levels, and emphasize positive emotional experiences during AI use. Adopting a "human-machine symbiosis" model can foster active learning, personalized learning pathways, and the development of students' digital efficacy and innovation capabilities.},
}
RevDate: 2024-10-26
CmpDate: 2024-10-26
Integrative Omics Strategies for Understanding and Combating Brown Planthopper Virulence in Rice Production: A Review.
International journal of molecular sciences, 25(20): pii:ijms252010981.
The brown planthopper (Nilaparvata lugens, BPH) is a serious insect pest responsible for causing immense economic losses to rice growers around the globe. The development of high-throughput sequencing technologies has significantly improved the research on this pest, and its genome structure, gene expression profiles, and host-plant interactions are being unveiled. The integration of genomic sequencing, transcriptomics, proteomics, and metabolomics has greatly increased our understanding of the biological characteristics of planthoppers, which will benefit the identification of resistant rice varieties and strategies for their control. Strategies like more optimal genome assembly and single-cell RNA-seq help to update our knowledge of gene control structure and cell type-specific usage, shedding light on how planthoppers adjust as well. However, to date, a comprehensive genome-wide investigation of the genetic interactions and population dynamics of BPHs has yet to be exhaustively performed using these next-generation omics technologies. This review summarizes the recent advances and new perspectives regarding the use of omics data for the BPH, with specific emphasis on the integration of both fields to help develop more sustainable pest management strategies. These findings, in combination with those of post-transcriptional and translational modifications involving non-coding RNAs as well as epigenetic variations, further detail intricate host-brown planthopper interaction dynamics, especially regarding resistant rice varieties. Finally, the symbiogenesis of the symbiotic microbial community in a planthopper can be characterized through metagenomic approaches, and its importance in enhancing virulence traits would offer novel opportunities for plant protection by manipulating host-microbe interactions. The concerted diverse omics approaches collectively identified the holistic and complex mechanisms of virulence variation in BPHs, which enables efficient deployment into rice resistance breeding as well as sustainable pest management.
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@article {pmid39456764,
year = {2024},
author = {Wang, X and Wang, Y and Yang, H and Liu, F and Cai, Y and Xiao, J and Fu, Q and Wan, P},
title = {Integrative Omics Strategies for Understanding and Combating Brown Planthopper Virulence in Rice Production: A Review.},
journal = {International journal of molecular sciences},
volume = {25},
number = {20},
pages = {},
doi = {10.3390/ijms252010981},
pmid = {39456764},
issn = {1422-0067},
support = {CPSIBRF-CNRRI-202406//Fundamental Research Funds for Central Public Welfare Research Institute/ ; 2021YFD1401100//National Key Research and Development Program of China/ ; LY22C140008//Zhejiang Provincial Natural Science Foundation of China/ ; CARS-01//China Agriculture Research System/ ; CAAS-ASTIP-2021-CNRRI//Rice Pest Management Research Group of the Agri-cultural Science and Technology Innovation Program of China Academy of Agricultural Science/ ; },
mesh = {*Oryza/parasitology/genetics/microbiology ; *Hemiptera/genetics/pathogenicity ; Animals ; Genomics/methods ; Plant Diseases/parasitology/genetics/microbiology ; Virulence/genetics ; Proteomics/methods ; Metabolomics/methods ; Transcriptome ; Multiomics ; },
abstract = {The brown planthopper (Nilaparvata lugens, BPH) is a serious insect pest responsible for causing immense economic losses to rice growers around the globe. The development of high-throughput sequencing technologies has significantly improved the research on this pest, and its genome structure, gene expression profiles, and host-plant interactions are being unveiled. The integration of genomic sequencing, transcriptomics, proteomics, and metabolomics has greatly increased our understanding of the biological characteristics of planthoppers, which will benefit the identification of resistant rice varieties and strategies for their control. Strategies like more optimal genome assembly and single-cell RNA-seq help to update our knowledge of gene control structure and cell type-specific usage, shedding light on how planthoppers adjust as well. However, to date, a comprehensive genome-wide investigation of the genetic interactions and population dynamics of BPHs has yet to be exhaustively performed using these next-generation omics technologies. This review summarizes the recent advances and new perspectives regarding the use of omics data for the BPH, with specific emphasis on the integration of both fields to help develop more sustainable pest management strategies. These findings, in combination with those of post-transcriptional and translational modifications involving non-coding RNAs as well as epigenetic variations, further detail intricate host-brown planthopper interaction dynamics, especially regarding resistant rice varieties. Finally, the symbiogenesis of the symbiotic microbial community in a planthopper can be characterized through metagenomic approaches, and its importance in enhancing virulence traits would offer novel opportunities for plant protection by manipulating host-microbe interactions. The concerted diverse omics approaches collectively identified the holistic and complex mechanisms of virulence variation in BPHs, which enables efficient deployment into rice resistance breeding as well as sustainable pest management.},
}
MeSH Terms:
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hide MeSH Terms
*Oryza/parasitology/genetics/microbiology
*Hemiptera/genetics/pathogenicity
Animals
Genomics/methods
Plant Diseases/parasitology/genetics/microbiology
Virulence/genetics
Proteomics/methods
Metabolomics/methods
Transcriptome
Multiomics
RevDate: 2024-10-26
Photophysiological and Oxidative Responses of the Symbiotic Estuarine Anemone Anthopleura hermaphroditica to the Impact of UV Radiation and Salinity: Field and Laboratory Approaches.
Antioxidants (Basel, Switzerland), 13(10): pii:antiox13101239.
The estuarine anemone Anthopleura hermaphroditica and its symbiont Philozoon anthopleurum are continuously exposed to intense fluctuations in solar radiation and salinity owing to tidal changes. The aim of this study was to evaluate the effects of the tidal cycle, solar radiation, and salinity fluctuations on the photosynthetic and cellular responses (lipid peroxidation, total phenolic compounds, and antioxidant activity) of the symbiont complex over a 24 h period in the Quempillén River Estuary. Additionally, laboratory experiments were conducted to determine the specific photobiological responses to photosynthetically active radiation (PAR), ultraviolet radiation (UVR), and salinity. Our field results showed that the photosynthetic parameters of the symbiont complex decreased with increasing ambient radiation; however, no relationship was observed with changes in salinity. Increased peroxidative damage, total phenolic compound levels, and antioxidant activity were mainly related to increased UVR and, to a lesser extent, PAR. During the dark period, only PAR-exposed organisms returned to the basal levels of photosynthesis and cell damage. Laboratory exposure confirmed the deleterious effects of UVR on the photosynthetic response. The present study suggests that the ability of A. hermaphroditica to acclimate to natural radiation stress is mediated by the concerted action of various physiological mechanisms that occur at different times of the day, under varying levels of environmental stress.
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@article {pmid39456492,
year = {2024},
author = {Cruces, E and Cubillos, VM and Ramírez-Kushel, E and Montory, JA and Mardones, DA and Chaparro, OR and Paredes, FJ and Echeverría-Pérez, I and Salas-Yanquin, LP and Büchner-Miranda, JA},
title = {Photophysiological and Oxidative Responses of the Symbiotic Estuarine Anemone Anthopleura hermaphroditica to the Impact of UV Radiation and Salinity: Field and Laboratory Approaches.},
journal = {Antioxidants (Basel, Switzerland)},
volume = {13},
number = {10},
pages = {},
doi = {10.3390/antiox13101239},
pmid = {39456492},
issn = {2076-3921},
support = {1221545//FONDECYT-ANID/ ; 1190875//FONDECYT-ANID/ ; 1241296//FONDECYT-ANID/ ; },
abstract = {The estuarine anemone Anthopleura hermaphroditica and its symbiont Philozoon anthopleurum are continuously exposed to intense fluctuations in solar radiation and salinity owing to tidal changes. The aim of this study was to evaluate the effects of the tidal cycle, solar radiation, and salinity fluctuations on the photosynthetic and cellular responses (lipid peroxidation, total phenolic compounds, and antioxidant activity) of the symbiont complex over a 24 h period in the Quempillén River Estuary. Additionally, laboratory experiments were conducted to determine the specific photobiological responses to photosynthetically active radiation (PAR), ultraviolet radiation (UVR), and salinity. Our field results showed that the photosynthetic parameters of the symbiont complex decreased with increasing ambient radiation; however, no relationship was observed with changes in salinity. Increased peroxidative damage, total phenolic compound levels, and antioxidant activity were mainly related to increased UVR and, to a lesser extent, PAR. During the dark period, only PAR-exposed organisms returned to the basal levels of photosynthesis and cell damage. Laboratory exposure confirmed the deleterious effects of UVR on the photosynthetic response. The present study suggests that the ability of A. hermaphroditica to acclimate to natural radiation stress is mediated by the concerted action of various physiological mechanisms that occur at different times of the day, under varying levels of environmental stress.},
}
RevDate: 2024-10-26
Functional Properties and Sensory Quality of Kombucha Analogs Based on Herbal Infusions.
Antioxidants (Basel, Switzerland), 13(10): pii:antiox13101191.
Traditionally, kombucha is produced by the fermentation of black or green tea infusions with the use of SCOBY (Symbiotic Culture of Bacteria and Yeasts). However, SCOBY exhibits the ability to ferment other substrates as well, which can be used to create novel products with new sensory and health-promoting properties. This paper investigates the antioxidant activity, chemical composition, and sensory properties of mint, nettle, and blackcurrant leaf-based kombucha analogs. It has been demonstrated that the fermentation process with SCOBY significantly influenced (p ≤ 0.05) sugar, organic acids, and mineral contents, with the increase in iron, magnesium, and calcium amounts in all tested herbal kombucha. The study shows that the type of herb infusion has a significant influence on the parameters associated with antioxidant potential. The fermentation with SCOBY resulted in an increase in antioxidant activity as measured by the superoxide anion radical (O2[•-]) inhibition of all three tested herbal infusions, with the greatest changes observed in nettle kombucha. Herbal kombucha was characterized by significantly increased total phenolic content as determined by Folin's reagent and a changed phenolic compound profile by LC-MS/MS (liquid chromatography with tandem mass spectrometry) in comparison to nonfermented infusions. Very high sensory scores were achieved for fermented mint and blackcurrant-based kombucha.
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@article {pmid39456445,
year = {2024},
author = {Czarnowska-Kujawska, M and Klepacka, J and Starowicz, M and Lesińska, P},
title = {Functional Properties and Sensory Quality of Kombucha Analogs Based on Herbal Infusions.},
journal = {Antioxidants (Basel, Switzerland)},
volume = {13},
number = {10},
pages = {},
doi = {10.3390/antiox13101191},
pmid = {39456445},
issn = {2076-3921},
support = {the Regional Initiative of Excellence Program//This research was funded by the Minister of Science under "the Regional Initiative of Excellence Program"./ ; },
abstract = {Traditionally, kombucha is produced by the fermentation of black or green tea infusions with the use of SCOBY (Symbiotic Culture of Bacteria and Yeasts). However, SCOBY exhibits the ability to ferment other substrates as well, which can be used to create novel products with new sensory and health-promoting properties. This paper investigates the antioxidant activity, chemical composition, and sensory properties of mint, nettle, and blackcurrant leaf-based kombucha analogs. It has been demonstrated that the fermentation process with SCOBY significantly influenced (p ≤ 0.05) sugar, organic acids, and mineral contents, with the increase in iron, magnesium, and calcium amounts in all tested herbal kombucha. The study shows that the type of herb infusion has a significant influence on the parameters associated with antioxidant potential. The fermentation with SCOBY resulted in an increase in antioxidant activity as measured by the superoxide anion radical (O2[•-]) inhibition of all three tested herbal infusions, with the greatest changes observed in nettle kombucha. Herbal kombucha was characterized by significantly increased total phenolic content as determined by Folin's reagent and a changed phenolic compound profile by LC-MS/MS (liquid chromatography with tandem mass spectrometry) in comparison to nonfermented infusions. Very high sensory scores were achieved for fermented mint and blackcurrant-based kombucha.},
}
RevDate: 2024-10-26
Integration of fungal transcriptomics and metabolomics provides insights into the early interaction between the ORM fungus Tulasnella sp. and the orchid Serapias vomeracea seeds.
IMA fungus, 15(1):31.
In nature, germination of orchid seeds and early plant development rely on a symbiotic association with orchid mycorrhizal (ORM) fungi. These fungi provide the host with the necessary nutrients and facilitate the transition from embryos to protocorms. Despite recent advances in omics technologies, our understanding of this symbiosis remains limited, particularly during the initial stages of the interaction. To address this gap, we employed transcriptomics and metabolomics to investigate the early responses occurring in the mycorrhizal fungus Tulasnella sp. isolate SV6 when co-cultivated with orchid seeds of Serapias vomeracea. The integration of data from gene expression and metabolite profiling revealed the activation of some fungal signalling pathways before the establishment of the symbiosis. Prior to seed contact, an indole-related metabolite was produced by the fungus, and significant changes in the fungal lipid profile occurred throughout the symbiotic process. Additionally, the expression of plant cell wall-degrading enzymes (PCWDEs) was observed during the pre-symbiotic stage, as the fungus approached the seeds, along with changes in amino acid metabolism. Thus, the dual-omics approach employed in this study yielded novel insights into the symbiotic relationship between orchids and ORM fungi and suggest that the ORM fungus responds to the presence of the orchid seeds prior to contact.
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@article {pmid39456087,
year = {2024},
author = {De Rose, S and Sillo, F and Ghirardo, A and Perotto, S and Schnitzler, JP and Balestrini, R},
title = {Integration of fungal transcriptomics and metabolomics provides insights into the early interaction between the ORM fungus Tulasnella sp. and the orchid Serapias vomeracea seeds.},
journal = {IMA fungus},
volume = {15},
number = {1},
pages = {31},
pmid = {39456087},
issn = {2210-6340},
abstract = {In nature, germination of orchid seeds and early plant development rely on a symbiotic association with orchid mycorrhizal (ORM) fungi. These fungi provide the host with the necessary nutrients and facilitate the transition from embryos to protocorms. Despite recent advances in omics technologies, our understanding of this symbiosis remains limited, particularly during the initial stages of the interaction. To address this gap, we employed transcriptomics and metabolomics to investigate the early responses occurring in the mycorrhizal fungus Tulasnella sp. isolate SV6 when co-cultivated with orchid seeds of Serapias vomeracea. The integration of data from gene expression and metabolite profiling revealed the activation of some fungal signalling pathways before the establishment of the symbiosis. Prior to seed contact, an indole-related metabolite was produced by the fungus, and significant changes in the fungal lipid profile occurred throughout the symbiotic process. Additionally, the expression of plant cell wall-degrading enzymes (PCWDEs) was observed during the pre-symbiotic stage, as the fungus approached the seeds, along with changes in amino acid metabolism. Thus, the dual-omics approach employed in this study yielded novel insights into the symbiotic relationship between orchids and ORM fungi and suggest that the ORM fungus responds to the presence of the orchid seeds prior to contact.},
}
RevDate: 2024-10-25
CmpDate: 2024-10-26
Rethinking asexuality: the enigmatic case of functional sexual genes in Lepraria (Stereocaulaceae).
BMC genomics, 25(1):1003.
BACKGROUND: The ubiquity of sex across eukaryotes, given its high costs, strongly suggests it is evolutionarily advantageous. Asexual lineages can avoid, for example, the risks and energetic costs of recombination, but suffer short-term reductions in adaptive potential and long-term damage to genome integrity. Despite these costs, lichenized fungi have frequently evolved asexual reproduction, likely because it allows the retention of symbiotic algae across generations. The lichenized fungal genus Lepraria is thought to be exclusively asexual, while its sister genus Stereocaulon completes a sexual reproductive cycle. A comparison of sister sexual and asexual clades should shed light on the evolution of asexuality in lichens in general, as well as the apparent long-term maintenance of asexuality in Lepraria, specifically.
RESULTS: In this study, we assembled and annotated representative long-read genomes from the putatively asexual Lepraria genus and its sexual sister genus Stereocaulon, and added short-read assemblies from an additional 22 individuals across both genera. Comparative genomic analyses revealed that both genera were heterothallic, with intact mating-type loci of both idiomorphs present across each genus. Additionally, we identified and assessed 29 genes involved in meiosis and mitosis and 45 genes that contribute to formation of fungal sexual reproductive structures (ascomata). All genes were present and appeared functional in nearly all Lepraria, and we failed to identify a general pattern of relaxation of selection on these genes across the Lepraria lineage. Together, these results suggest that Lepraria may be capable of sexual reproduction, including mate recognition, meiosis, and production of ascomata.
CONCLUSIONS: Despite apparent maintenance of machinery essential for fungal sex, over 200 years of careful observations by lichenologists have produced no evidence of canonical sexual reproduction in Lepraria. We suggest that Lepraria may have instead evolved a form of parasexual reproduction, perhaps by repurposing MAT and meiosis-specific genes. This may, in turn, allow these lichenized fungi to avoid long-term consequences of asexuality, while maintaining the benefit of an unbroken bond with their algal symbionts.
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@article {pmid39455957,
year = {2024},
author = {Doellman, MM and Sun, Y and Barcenas-Peña, A and Lumbsch, HT and Grewe, F},
title = {Rethinking asexuality: the enigmatic case of functional sexual genes in Lepraria (Stereocaulaceae).},
journal = {BMC genomics},
volume = {25},
number = {1},
pages = {1003},
pmid = {39455957},
issn = {1471-2164},
support = {CTM2015-64728-C2-1-R//Spanish Ministry of Science/ ; },
mesh = {*Reproduction, Asexual/genetics ; Phylogeny ; Ascomycota/genetics/physiology ; Genome, Fungal ; Lichens/genetics ; Genes, Mating Type, Fungal/genetics ; Genomics ; },
abstract = {BACKGROUND: The ubiquity of sex across eukaryotes, given its high costs, strongly suggests it is evolutionarily advantageous. Asexual lineages can avoid, for example, the risks and energetic costs of recombination, but suffer short-term reductions in adaptive potential and long-term damage to genome integrity. Despite these costs, lichenized fungi have frequently evolved asexual reproduction, likely because it allows the retention of symbiotic algae across generations. The lichenized fungal genus Lepraria is thought to be exclusively asexual, while its sister genus Stereocaulon completes a sexual reproductive cycle. A comparison of sister sexual and asexual clades should shed light on the evolution of asexuality in lichens in general, as well as the apparent long-term maintenance of asexuality in Lepraria, specifically.
RESULTS: In this study, we assembled and annotated representative long-read genomes from the putatively asexual Lepraria genus and its sexual sister genus Stereocaulon, and added short-read assemblies from an additional 22 individuals across both genera. Comparative genomic analyses revealed that both genera were heterothallic, with intact mating-type loci of both idiomorphs present across each genus. Additionally, we identified and assessed 29 genes involved in meiosis and mitosis and 45 genes that contribute to formation of fungal sexual reproductive structures (ascomata). All genes were present and appeared functional in nearly all Lepraria, and we failed to identify a general pattern of relaxation of selection on these genes across the Lepraria lineage. Together, these results suggest that Lepraria may be capable of sexual reproduction, including mate recognition, meiosis, and production of ascomata.
CONCLUSIONS: Despite apparent maintenance of machinery essential for fungal sex, over 200 years of careful observations by lichenologists have produced no evidence of canonical sexual reproduction in Lepraria. We suggest that Lepraria may have instead evolved a form of parasexual reproduction, perhaps by repurposing MAT and meiosis-specific genes. This may, in turn, allow these lichenized fungi to avoid long-term consequences of asexuality, while maintaining the benefit of an unbroken bond with their algal symbionts.},
}
MeSH Terms:
show MeSH Terms
hide MeSH Terms
*Reproduction, Asexual/genetics
Phylogeny
Ascomycota/genetics/physiology
Genome, Fungal
Lichens/genetics
Genes, Mating Type, Fungal/genetics
Genomics
RevDate: 2024-10-25
CmpDate: 2024-10-25
Effects of urban green space habitats and tree species on ectomycorrhizal fungal diversity.
Scientific reports, 14(1):25369.
Ectomycorrhizal fungi (EMF) are key symbiotic microbial components for the growth and health of trees in urban greenspace habitats (UGSHs). However, the current understanding of EMF diversity in UGSHs remains poor. Therefore, in this study, using morphological classification and molecular identification, we aimed to investigate EMF diversity in three EMF host plants: Cedrus deodara in the roadside green belt, and C. deodara, Pinus massoniana, and Salix babylonica in the park roadside green belt, in Guiyang, China. A total of 62 EMF Operational Taxonomic Units (OTUs) were identified, including 13 EMF OTUs in the C. deodara roadside green belt, and 23, 31, and 9 EMF OTUs in the park green belts. C. deodara, P. massoniana, and S. babylonica were respectively identified in park green belts. Ascomycota and Basidiomycota were the dominant phylum in the EMF communities in roadside and park green habitat, respectively. The Shannon and Simpson indexes of the C. deodara EMF community in the park green belt were higher than those in the roadside green belt. EMF diversity of the tree species in the park green belt was P. massoniana > C. deodara > S. babylonica. Differences in EMF community diversity was observed among the different greening tree species in the UGSHs. UGSHs with different disturbance gradients had a significant impact on the EMF diversity of the same greening tree species. These results can be used as a scientific reference for optimizing the design and scientific management of UGSHs.
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@article {pmid39455594,
year = {2024},
author = {Lin, QC and Cen, YQ and Xu, M and Jiang, DD and Zhang, J},
title = {Effects of urban green space habitats and tree species on ectomycorrhizal fungal diversity.},
journal = {Scientific reports},
volume = {14},
number = {1},
pages = {25369},
pmid = {39455594},
issn = {2045-2322},
support = {31660150//Study on the driving mechanism of diversity and distribution pattern of ectomycorrhizal fungi in Pinus massoniana in Southwest China/ ; 31960234//Plant function of Pinus massoniana community Study on the spatio-temporal evolution characteristics and interaction mechanism of traits and environmental factors./ ; },
mesh = {*Mycorrhizae/genetics/physiology/classification ; *Trees/microbiology ; *Ecosystem ; *Biodiversity ; China ; Pinus/microbiology ; Soil Microbiology ; Cedrus/microbiology ; },
abstract = {Ectomycorrhizal fungi (EMF) are key symbiotic microbial components for the growth and health of trees in urban greenspace habitats (UGSHs). However, the current understanding of EMF diversity in UGSHs remains poor. Therefore, in this study, using morphological classification and molecular identification, we aimed to investigate EMF diversity in three EMF host plants: Cedrus deodara in the roadside green belt, and C. deodara, Pinus massoniana, and Salix babylonica in the park roadside green belt, in Guiyang, China. A total of 62 EMF Operational Taxonomic Units (OTUs) were identified, including 13 EMF OTUs in the C. deodara roadside green belt, and 23, 31, and 9 EMF OTUs in the park green belts. C. deodara, P. massoniana, and S. babylonica were respectively identified in park green belts. Ascomycota and Basidiomycota were the dominant phylum in the EMF communities in roadside and park green habitat, respectively. The Shannon and Simpson indexes of the C. deodara EMF community in the park green belt were higher than those in the roadside green belt. EMF diversity of the tree species in the park green belt was P. massoniana > C. deodara > S. babylonica. Differences in EMF community diversity was observed among the different greening tree species in the UGSHs. UGSHs with different disturbance gradients had a significant impact on the EMF diversity of the same greening tree species. These results can be used as a scientific reference for optimizing the design and scientific management of UGSHs.},
}
MeSH Terms:
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*Mycorrhizae/genetics/physiology/classification
*Trees/microbiology
*Ecosystem
*Biodiversity
China
Pinus/microbiology
Soil Microbiology
Cedrus/microbiology
RevDate: 2024-10-25
Response to shock load of titanium dioxide nanoparticles on aerobic granular sludge and algal-bacterial granular sludge processes.
NanoImpact pii:S2452-0748(24)00042-9 [Epub ahead of print].
Titanium dioxide nanoparticles (TiO2 NPs) are extensively used in various fields and can consequently be detected in wastewater, making it necessary to study their potential impacts on biological wastewater treatment processes. In this study, the shock-load impacts of TiO2 NPs were investigated at concentrations ranging between 1 and 200 mg L[-1] on nutrient removal, extracellular polymeric substances (EPSs), microbial activity in aerobic granular sludge (AGS), and algal-bacterial granular sludge (AB-AGS) bioreactors. The results indicated that low concentration (≤10 mg L[-1]) TiO2 NPs had no effect on microbial activity or the removal of chemical oxygen demand (COD), nitrogen, and phosphorus, due to the increased production of extracellular polymeric substances (EPSs) in the sludge. In contrast, the performance of both AGS and AB-AGS bioreactors gradually deteriorated as the concentration of TiO2 NPs in the influent increased to 50, 100, and 200 mg L[-1]. Specifically, the ammonia‑nitrogen removal rate in AGS decreased from 99.9 % to 88.6 %, while in AB-AGS it dropped to 91.3 % at 200 mg L[-1] TiO2 NPs. Furthermore, the nitrate‑nitrogen levels remained stable in AB-AGS, while NO3-N was detected in the effluent of AGS at 100 and 200 mg L[-1]. Microbial activities change similarly as smaller decrease in the specific ammonia uptake rate (SAUR) and specific nitrate uptake rate (SNUR) was found in AB-AGS compared to those in AGS. Overall, the algal-bacterial sludge exhibited higher resilience against TiO2 NPs, which was attributed to a) higher EPS volume, b) smaller decrease in LB-EPS, and c) the favorable protein to polysaccharide (PN/PS) ratio. This in turn, along with the symbiotic relationship between the algae and bacteria, mitigates the toxic effects of nanoparticles.
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@article {pmid39454679,
year = {2024},
author = {Kedves, A and Yavuz, Ç and Kedves, O and Haspel, H and Kónya, Z},
title = {Response to shock load of titanium dioxide nanoparticles on aerobic granular sludge and algal-bacterial granular sludge processes.},
journal = {NanoImpact},
volume = {},
number = {},
pages = {100532},
doi = {10.1016/j.impact.2024.100532},
pmid = {39454679},
issn = {2452-0748},
abstract = {Titanium dioxide nanoparticles (TiO2 NPs) are extensively used in various fields and can consequently be detected in wastewater, making it necessary to study their potential impacts on biological wastewater treatment processes. In this study, the shock-load impacts of TiO2 NPs were investigated at concentrations ranging between 1 and 200 mg L[-1] on nutrient removal, extracellular polymeric substances (EPSs), microbial activity in aerobic granular sludge (AGS), and algal-bacterial granular sludge (AB-AGS) bioreactors. The results indicated that low concentration (≤10 mg L[-1]) TiO2 NPs had no effect on microbial activity or the removal of chemical oxygen demand (COD), nitrogen, and phosphorus, due to the increased production of extracellular polymeric substances (EPSs) in the sludge. In contrast, the performance of both AGS and AB-AGS bioreactors gradually deteriorated as the concentration of TiO2 NPs in the influent increased to 50, 100, and 200 mg L[-1]. Specifically, the ammonia‑nitrogen removal rate in AGS decreased from 99.9 % to 88.6 %, while in AB-AGS it dropped to 91.3 % at 200 mg L[-1] TiO2 NPs. Furthermore, the nitrate‑nitrogen levels remained stable in AB-AGS, while NO3-N was detected in the effluent of AGS at 100 and 200 mg L[-1]. Microbial activities change similarly as smaller decrease in the specific ammonia uptake rate (SAUR) and specific nitrate uptake rate (SNUR) was found in AB-AGS compared to those in AGS. Overall, the algal-bacterial sludge exhibited higher resilience against TiO2 NPs, which was attributed to a) higher EPS volume, b) smaller decrease in LB-EPS, and c) the favorable protein to polysaccharide (PN/PS) ratio. This in turn, along with the symbiotic relationship between the algae and bacteria, mitigates the toxic effects of nanoparticles.},
}
RevDate: 2024-10-25
Beatie Wolfe: Designing for the Experience of Analog-Digital Symbiosis.
IEEE computer graphics and applications, 44(5):85-92.
We have enjoyed getting to know Beatie Wolfe after finding her work to be compelling for its consistent ability to use art and communication to build bridges between different societal worlds. Beatie is a multimedia artist who started her career reimagining the ceremonial experiences of analog music formats, creating a new series of retro-future designs for the digital age, and now perfects an artistic process, which melds art and science and other disciplines to create stunning multifaceted installations that draw huge enthusiastic audiences when displayed in public.
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@article {pmid39453782,
year = {2024},
author = {Wolfe, B and Campbell, BD and Samsel, F and Campbell, BD and Samsel, F},
title = {Beatie Wolfe: Designing for the Experience of Analog-Digital Symbiosis.},
journal = {IEEE computer graphics and applications},
volume = {44},
number = {5},
pages = {85-92},
doi = {10.1109/MCG.2024.3428588},
pmid = {39453782},
issn = {1558-1756},
abstract = {We have enjoyed getting to know Beatie Wolfe after finding her work to be compelling for its consistent ability to use art and communication to build bridges between different societal worlds. Beatie is a multimedia artist who started her career reimagining the ceremonial experiences of analog music formats, creating a new series of retro-future designs for the digital age, and now perfects an artistic process, which melds art and science and other disciplines to create stunning multifaceted installations that draw huge enthusiastic audiences when displayed in public.},
}
RevDate: 2024-10-26
On the Origins of Symbiotic Fungi in Carmine Cochineals and Their Function in the Digestion of Plant Polysaccharides.
Insects, 15(10):.
The cochineal insect Dactylopius coccus Costa (Hemiptera) has cultural and economic value because it produces carminic acid that is used commercially. In this study, distinct fungi were cultured from dissected tissue and identified as Penicillium, Coniochaeta, Arthrinium, Cladosporium, Microascus, Aspergillus, and Periconia. Fungi were microscopically observed inside cochineals in the gut, fat body, and ovaries. Since cochineals spend their lives attached to cactus leaves and use the sap as feed, they can obtain fungi from cacti plants. Indeed, we obtained Penicillium, Aspergillus, and Cladosporium fungi from cacti that were identical to those inside cochineals, supporting their plant origin. Fungi could be responsible for the degrading activities in the insect guts, since cellulase, pectinase, and amylase enzymatic activities in insect guts decreased in fungicide-treated cochineals. Our findings set the basis for the further study of the interactions between insects, fungi, and their host plants.
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@article {pmid39452359,
year = {2024},
author = {González-Román, P and Hernández-Oaxaca, D and Bustamante-Brito, R and Rogel, MA and Martínez-Romero, E},
title = {On the Origins of Symbiotic Fungi in Carmine Cochineals and Their Function in the Digestion of Plant Polysaccharides.},
journal = {Insects},
volume = {15},
number = {10},
pages = {},
pmid = {39452359},
issn = {2075-4450},
abstract = {The cochineal insect Dactylopius coccus Costa (Hemiptera) has cultural and economic value because it produces carminic acid that is used commercially. In this study, distinct fungi were cultured from dissected tissue and identified as Penicillium, Coniochaeta, Arthrinium, Cladosporium, Microascus, Aspergillus, and Periconia. Fungi were microscopically observed inside cochineals in the gut, fat body, and ovaries. Since cochineals spend their lives attached to cactus leaves and use the sap as feed, they can obtain fungi from cacti plants. Indeed, we obtained Penicillium, Aspergillus, and Cladosporium fungi from cacti that were identical to those inside cochineals, supporting their plant origin. Fungi could be responsible for the degrading activities in the insect guts, since cellulase, pectinase, and amylase enzymatic activities in insect guts decreased in fungicide-treated cochineals. Our findings set the basis for the further study of the interactions between insects, fungi, and their host plants.},
}
RevDate: 2024-10-25
CmpDate: 2024-10-25
[The human microbiome proofed by the Anthropocene: from correlation to causality and intervention].
Medecine sciences : M/S, 40(10):757-765.
The deleterious effects of human activities on biodiversity in the vegetal and animal world, and on climate changes are now well-established facts. However, little is yet known on the impact of human activities on microbial diversity on the planet and more specifically on the human microbiota Large implementation of metagenomics allows exaustive microbial cataloguing with broad spatio-temporal resolution of human microbiota. A reduction in bacterial richness and diversity in the human microbiota, particularly in the intestinal tract, is now established and particularly obvious in the most industrialized regions of the planet. Massive, uncontrolled use of antibiotics, drastic changes in traditional food habits and some elements of the "global exposome" that remain to identify are usually considered as stressors accounting for this situation of "missing microbes". As a consequence, a dysbiotic situation develops, a "dysbiosis" being characterized by the erosion of the central core of shared bacterial species across individuals and the development of opportunistic "pathobionts" in response to a weaker barrier capacity of these impoverished microbiota. The current challenge is to establish a causality link between the extension of these dysbiotic situations and the steady emergence of epidemic, non-communicable diseases such as asthma, allergy, obesity, diabetes, autoimmune diseases and some cancers. Experimental animal models combined with controlled, prospective clinical interventions are in demand to consolidate causality links, with the understanding that in the deciphering of the mechanisms of alteration of the human-microbiome symbiosis resides a novel exciting chapter of medicine: "microbial medicine".
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@article {pmid39450961,
year = {2024},
author = {Sansonetti, PJ and Doré, J},
title = {[The human microbiome proofed by the Anthropocene: from correlation to causality and intervention].},
journal = {Medecine sciences : M/S},
volume = {40},
number = {10},
pages = {757-765},
doi = {10.1051/medsci/2024121},
pmid = {39450961},
issn = {1958-5381},
mesh = {Humans ; *Microbiota/physiology ; Animals ; *Gastrointestinal Microbiome/physiology ; *Dysbiosis/microbiology ; Biodiversity ; Causality ; Climate Change ; },
abstract = {The deleterious effects of human activities on biodiversity in the vegetal and animal world, and on climate changes are now well-established facts. However, little is yet known on the impact of human activities on microbial diversity on the planet and more specifically on the human microbiota Large implementation of metagenomics allows exaustive microbial cataloguing with broad spatio-temporal resolution of human microbiota. A reduction in bacterial richness and diversity in the human microbiota, particularly in the intestinal tract, is now established and particularly obvious in the most industrialized regions of the planet. Massive, uncontrolled use of antibiotics, drastic changes in traditional food habits and some elements of the "global exposome" that remain to identify are usually considered as stressors accounting for this situation of "missing microbes". As a consequence, a dysbiotic situation develops, a "dysbiosis" being characterized by the erosion of the central core of shared bacterial species across individuals and the development of opportunistic "pathobionts" in response to a weaker barrier capacity of these impoverished microbiota. The current challenge is to establish a causality link between the extension of these dysbiotic situations and the steady emergence of epidemic, non-communicable diseases such as asthma, allergy, obesity, diabetes, autoimmune diseases and some cancers. Experimental animal models combined with controlled, prospective clinical interventions are in demand to consolidate causality links, with the understanding that in the deciphering of the mechanisms of alteration of the human-microbiome symbiosis resides a novel exciting chapter of medicine: "microbial medicine".},
}
MeSH Terms:
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Humans
*Microbiota/physiology
Animals
*Gastrointestinal Microbiome/physiology
*Dysbiosis/microbiology
Biodiversity
Causality
Climate Change
RevDate: 2024-10-25
Exopolysaccharide is detrimental for the symbiotic performance of Sinorhizobium fredii HH103 mutants with a truncated lipopolysaccharide core.
The Biochemical journal pii:235163 [Epub ahead of print].
The nitrogen-fixing rhizobia-legume symbiosis relies on a complex interchange of molecular signals between the two partners during the whole interaction. On the bacterial side, different surface polysaccharides, such as lipopolysaccharide (LPS) and exopolysaccharide (EPS), might play important roles for the success of the interaction. In a previous work we studied two Sinorhizobium fredii HH103 mutants affected in the rkpK and lpsL genes, which are responsible for the production of glucuronic acid and galacturonic acid, respectively. Both mutants produced an altered LPS, and the rkpK mutant, in addition, lacked EPS. These mutants were differently affected in symbiosis with Glycine max and Vigna unguiculata, with the lpsL mutant showing a stronger impairment than the rkpK mutant. In the present work we have further investigated the LPS structure and the symbiotic abilities of the HH103 lpsL and rkpK mutants. We demonstrate that both strains produce the same LPS, with a truncated core oligosaccharide devoid of uronic acids. We show that the symbiotic performance of the lpsL mutant with Macroptilium atropurpureum and Glycyrrhiza uralensis is worse than that of the rkpK mutant. Introduction of an exoA mutation (which avoids EPS production) in HH103 lpsL improved its symbiotic performance with G. max, M. atropurpureum, and G. uralensis to the level exhibited by HH103 rkpK, suggesting that the presence of EPS might hide the truncated LPS produced by the former mutant.
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@article {pmid39450641,
year = {2024},
author = {Fuentes-Romero, F and Mercogliano, M and De Chiara, S and Alías-Villegas, C and Navarro-Gómez, P and Acosta-Jurado, S and Silipo, A and Medina, C and Rodríguez-Carvajal, MÁ and Dardanelli, MS and Ruiz-Sainz, JE and López-Baena, FJ and Molinaro, A and Vinardell, JM and Di Lorenzo, F},
title = {Exopolysaccharide is detrimental for the symbiotic performance of Sinorhizobium fredii HH103 mutants with a truncated lipopolysaccharide core.},
journal = {The Biochemical journal},
volume = {},
number = {},
pages = {},
doi = {10.1042/BCJ20240599},
pmid = {39450641},
issn = {1470-8728},
abstract = {The nitrogen-fixing rhizobia-legume symbiosis relies on a complex interchange of molecular signals between the two partners during the whole interaction. On the bacterial side, different surface polysaccharides, such as lipopolysaccharide (LPS) and exopolysaccharide (EPS), might play important roles for the success of the interaction. In a previous work we studied two Sinorhizobium fredii HH103 mutants affected in the rkpK and lpsL genes, which are responsible for the production of glucuronic acid and galacturonic acid, respectively. Both mutants produced an altered LPS, and the rkpK mutant, in addition, lacked EPS. These mutants were differently affected in symbiosis with Glycine max and Vigna unguiculata, with the lpsL mutant showing a stronger impairment than the rkpK mutant. In the present work we have further investigated the LPS structure and the symbiotic abilities of the HH103 lpsL and rkpK mutants. We demonstrate that both strains produce the same LPS, with a truncated core oligosaccharide devoid of uronic acids. We show that the symbiotic performance of the lpsL mutant with Macroptilium atropurpureum and Glycyrrhiza uralensis is worse than that of the rkpK mutant. Introduction of an exoA mutation (which avoids EPS production) in HH103 lpsL improved its symbiotic performance with G. max, M. atropurpureum, and G. uralensis to the level exhibited by HH103 rkpK, suggesting that the presence of EPS might hide the truncated LPS produced by the former mutant.},
}
RevDate: 2024-10-26
Gene expression signatures of mutualism and pathogenesis in flax roots.
Frontiers in plant science, 15:1415082.
INTRODUCTION: Fusarium wilt, a devastating soil-borne fungal disease in flax (Linum usitatissimum), is caused by Fusarium oxysporum f. sp. lini, a hemibiotrophic plant pathogen that penetrates plant roots. There are several reports of the molecular response of L. usitatissimum to F. oxysporum f. sp. lini; however, comparisons of the effects of mutualistic and pathogenic fungi on plants are more limited.
METHODS: In this study, we have integrated phenotyping and RNA-Seq approaches to examine the response of flax to F. oxysporum f.sp. lini and to a mutualistic arbuscular mycorrhizal fungus (AMF) Rhizoglomus irregulare. R. irregulare is a common soil fungus and also widely used as a commercial inoculant to improve plant growth. We measured flax growth parameters after plant inoculation with each or both fungi, in comparison with non-inoculated control. We performed transcriptome analysis of root tissues collected at 9 and 14 days post-inoculation.
RESULTS: We identified several differentially expressed genes (DEGs) in response to pathogenic and mutualistic fungi. These included genes related to ethylene and salicylic acid biosynthesis, carbohydrate binding, oxidoreductases, and sugar transmembrane transporters. Genes related to calcium signaling, nutrient transport, lipid metabolism, cell wall, and polysaccharide-modifying were up-regulated by R. irregulare; however, the same genes were down-regulated by F. oxysporum f. sp. lini when treated independently. In the combined treatment, genes related to cell wall modifications, hormone regulation and nutrient uptake were up-regulated. These results suggest that inoculation with R. irregulare reduced gene expression related to F. oxysporum f. sp. lini infection, leading to a reduced response to the pathogen. In response to AMF, flax prioritized mutualism-related gene expression over defense, reversing the growth inhibition caused by F. oxysporum f. sp.lini in the combined treatment.
DISCUSSION: This research provides insights into the protective effects of AMF, revealing the pre-symbiotic gene expression profile of flax in response to mutualism in comparison with pathogenicity. Potential target genes for crop improvement were identified, especially defense related genes.
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@article {pmid39450082,
year = {2024},
author = {Quintans, ILADCR and Vukicevich, E and Kokkoris, V and Packard, E and Adhikary, D and Hart, MM and Deyholos, MK},
title = {Gene expression signatures of mutualism and pathogenesis in flax roots.},
journal = {Frontiers in plant science},
volume = {15},
number = {},
pages = {1415082},
pmid = {39450082},
issn = {1664-462X},
abstract = {INTRODUCTION: Fusarium wilt, a devastating soil-borne fungal disease in flax (Linum usitatissimum), is caused by Fusarium oxysporum f. sp. lini, a hemibiotrophic plant pathogen that penetrates plant roots. There are several reports of the molecular response of L. usitatissimum to F. oxysporum f. sp. lini; however, comparisons of the effects of mutualistic and pathogenic fungi on plants are more limited.
METHODS: In this study, we have integrated phenotyping and RNA-Seq approaches to examine the response of flax to F. oxysporum f.sp. lini and to a mutualistic arbuscular mycorrhizal fungus (AMF) Rhizoglomus irregulare. R. irregulare is a common soil fungus and also widely used as a commercial inoculant to improve plant growth. We measured flax growth parameters after plant inoculation with each or both fungi, in comparison with non-inoculated control. We performed transcriptome analysis of root tissues collected at 9 and 14 days post-inoculation.
RESULTS: We identified several differentially expressed genes (DEGs) in response to pathogenic and mutualistic fungi. These included genes related to ethylene and salicylic acid biosynthesis, carbohydrate binding, oxidoreductases, and sugar transmembrane transporters. Genes related to calcium signaling, nutrient transport, lipid metabolism, cell wall, and polysaccharide-modifying were up-regulated by R. irregulare; however, the same genes were down-regulated by F. oxysporum f. sp. lini when treated independently. In the combined treatment, genes related to cell wall modifications, hormone regulation and nutrient uptake were up-regulated. These results suggest that inoculation with R. irregulare reduced gene expression related to F. oxysporum f. sp. lini infection, leading to a reduced response to the pathogen. In response to AMF, flax prioritized mutualism-related gene expression over defense, reversing the growth inhibition caused by F. oxysporum f. sp.lini in the combined treatment.
DISCUSSION: This research provides insights into the protective effects of AMF, revealing the pre-symbiotic gene expression profile of flax in response to mutualism in comparison with pathogenicity. Potential target genes for crop improvement were identified, especially defense related genes.},
}
RevDate: 2024-10-25
CmpDate: 2024-10-25
[Using metabolomics to explore the effects of epigenetic-modification strategies on the metabolites of Acanthus ilicifolius L. endophytic fungi against ovarian cancer].
Se pu = Chinese journal of chromatography, 42(11):1015-1023.
Ovarian cancer is a serious threat to women's health and safety. So far, people have discovered more than 130 small molecule compounds of natural origin for anti-tumor, of which approximately 50% are of microbial origin. The Acanthus ilicifolius L. species is primarily distributed in the Guangdong, Hainan, and Guangxi regions of China and grows in tidally accessible coastal areas. Recent studies have revealed that Acanthus ilicifolius L. extracts are endowed with a range of pharmacological properties, including anti-inflammatory, hepatoprotective, antioxidant, and antitumor activities. Endophytic fungi are commonly found in the healthy tissue and organs of medicinal plants. These fungi and the plants they inhabit form mutually beneficial symbiotic relationships. Endophytic fungi produce a series of secondary metabolites, with active substances having shown great economic value and applications prospects in drug research and development as well as for the biological control of plant diseases. Secondary metabolites production by endophytic fungi is regulated by specific gene clusters, and several techniques have been used to stimulate the secondary metabolic processes of fungi, including epigenetic-modification and OSMAC (one strain many compounds) strategies, co-culturing, and gene modification. Among these, epigenetic modification has been shown to be effective; this strategy involves the addition of small-molecule epigenetic modifiers to the culture medium, thereby activating silenced biosynthetic gene clusters without altering the DNA sequences of the fungi. This approach facilitates the expression of silenced genes in endophytic fungi, thereby increasing the number and diversity of secondary metabolites. Furthermore, it assists in overcoming the inhibition of microbial secondary-metabolite synthesis under laboratory conditions, and enhances silenced-gene expressions. The advent of novel analytical techniques and bioinformatics has provided a comprehensive, multifaceted, and holistic understanding of fungal metabolism through the development of metabolomics as a research platform. However, few studies have combined anti-ovarian cancer-activity screening with metabolomic approaches in the search for activity-differentiating metabolites from endophytic fungi under the intervention of epigenetic modifiers. Herein, we investigated the impact of epigenetic modifiers on the secondary metabolites of the endophytic Diaporthe goulteri fungus from Acanthus ilicifolius L. to determine their potential anti-ovarian cancer activities. Crude extracts were obtained by controlling three variables: the number of fermentation days, the type of epigenetic modifier, and its concentration, with activities screened using the CCK-8 (cell counting kit-8) method. Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was subsequently employed for non-targeted metabolomic analysis. A multivariate statistical analysis model was constructed using principal component analysis and orthogonal partial least squares-discriminant analysis, which combines model and variable importance projection, with qualitative screening performed and significant changes (variable importance in the projection (VIP)≥1; P<0. 05) determined. Fifteen differential metabolites were identified in the fungal and epigenetic modification group, primarily comprising polyketides, amino acids, derivatives, alkaloids, and organic acids, including prenderol, glycine, valine, 2-ethylcaproic acid, rubratoxin B, finasteride, 6-silaspiro[5.5]undecane, 1-(2-nitrophenoxy)octane, heptadecene, 1-pentadecene, 11-ketoetiocholanolone, 3-(1-ethyl-1,3,3-trimethyl-2,3-dihydro-1H-inden-5-yl)butanal, N[2]-benzoylarginine, tabutrex, (3aR,6S,6aS)-6-(4-hydroxy-2-methoxy-2-butanyl)-4,4-dimethylhexahydro-1(2H)-pentalenone, and 8-aminoquinoline. The expressions of prenderol, 1-(2-nitrophenoxy)octane, 3-(1-ethyl-1,3,3-trimethyl-2,3-dihydro-1H-inden-5-yl)butanal, N[2]-benzoylarginine, and 8-aminoquinoline were downregulated, whereas the expressions of the remaining 10 substances were upregulated. Polyketides were the main components that exhibited higher expressions. This study showed that latent active differential metabolites can be searched by combining anti-ovarian cancer-activity screening with metabolomics analysis, thereby providing a reference for the further development of Acanthus ilicifolius L. resources and the subsequent targeted isolation of active compounds.
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@article {pmid39449508,
year = {2024},
author = {Ma, XL and Cai, LY and Liu, YY and Xing, SP and Kang, L and Wei, X and Zhu, D},
title = {[Using metabolomics to explore the effects of epigenetic-modification strategies on the metabolites of Acanthus ilicifolius L. endophytic fungi against ovarian cancer].},
journal = {Se pu = Chinese journal of chromatography},
volume = {42},
number = {11},
pages = {1015-1023},
doi = {10.3724/SP.J.1123.2024.08002},
pmid = {39449508},
issn = {1872-2059},
mesh = {Female ; *Metabolomics ; *Endophytes/metabolism/chemistry ; Humans ; *Epigenesis, Genetic ; *Ovarian Neoplasms/microbiology ; Fungi/metabolism ; },
abstract = {Ovarian cancer is a serious threat to women's health and safety. So far, people have discovered more than 130 small molecule compounds of natural origin for anti-tumor, of which approximately 50% are of microbial origin. The Acanthus ilicifolius L. species is primarily distributed in the Guangdong, Hainan, and Guangxi regions of China and grows in tidally accessible coastal areas. Recent studies have revealed that Acanthus ilicifolius L. extracts are endowed with a range of pharmacological properties, including anti-inflammatory, hepatoprotective, antioxidant, and antitumor activities. Endophytic fungi are commonly found in the healthy tissue and organs of medicinal plants. These fungi and the plants they inhabit form mutually beneficial symbiotic relationships. Endophytic fungi produce a series of secondary metabolites, with active substances having shown great economic value and applications prospects in drug research and development as well as for the biological control of plant diseases. Secondary metabolites production by endophytic fungi is regulated by specific gene clusters, and several techniques have been used to stimulate the secondary metabolic processes of fungi, including epigenetic-modification and OSMAC (one strain many compounds) strategies, co-culturing, and gene modification. Among these, epigenetic modification has been shown to be effective; this strategy involves the addition of small-molecule epigenetic modifiers to the culture medium, thereby activating silenced biosynthetic gene clusters without altering the DNA sequences of the fungi. This approach facilitates the expression of silenced genes in endophytic fungi, thereby increasing the number and diversity of secondary metabolites. Furthermore, it assists in overcoming the inhibition of microbial secondary-metabolite synthesis under laboratory conditions, and enhances silenced-gene expressions. The advent of novel analytical techniques and bioinformatics has provided a comprehensive, multifaceted, and holistic understanding of fungal metabolism through the development of metabolomics as a research platform. However, few studies have combined anti-ovarian cancer-activity screening with metabolomic approaches in the search for activity-differentiating metabolites from endophytic fungi under the intervention of epigenetic modifiers. Herein, we investigated the impact of epigenetic modifiers on the secondary metabolites of the endophytic Diaporthe goulteri fungus from Acanthus ilicifolius L. to determine their potential anti-ovarian cancer activities. Crude extracts were obtained by controlling three variables: the number of fermentation days, the type of epigenetic modifier, and its concentration, with activities screened using the CCK-8 (cell counting kit-8) method. Ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was subsequently employed for non-targeted metabolomic analysis. A multivariate statistical analysis model was constructed using principal component analysis and orthogonal partial least squares-discriminant analysis, which combines model and variable importance projection, with qualitative screening performed and significant changes (variable importance in the projection (VIP)≥1; P<0. 05) determined. Fifteen differential metabolites were identified in the fungal and epigenetic modification group, primarily comprising polyketides, amino acids, derivatives, alkaloids, and organic acids, including prenderol, glycine, valine, 2-ethylcaproic acid, rubratoxin B, finasteride, 6-silaspiro[5.5]undecane, 1-(2-nitrophenoxy)octane, heptadecene, 1-pentadecene, 11-ketoetiocholanolone, 3-(1-ethyl-1,3,3-trimethyl-2,3-dihydro-1H-inden-5-yl)butanal, N[2]-benzoylarginine, tabutrex, (3aR,6S,6aS)-6-(4-hydroxy-2-methoxy-2-butanyl)-4,4-dimethylhexahydro-1(2H)-pentalenone, and 8-aminoquinoline. The expressions of prenderol, 1-(2-nitrophenoxy)octane, 3-(1-ethyl-1,3,3-trimethyl-2,3-dihydro-1H-inden-5-yl)butanal, N[2]-benzoylarginine, and 8-aminoquinoline were downregulated, whereas the expressions of the remaining 10 substances were upregulated. Polyketides were the main components that exhibited higher expressions. This study showed that latent active differential metabolites can be searched by combining anti-ovarian cancer-activity screening with metabolomics analysis, thereby providing a reference for the further development of Acanthus ilicifolius L. resources and the subsequent targeted isolation of active compounds.},
}
MeSH Terms:
show MeSH Terms
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Female
*Metabolomics
*Endophytes/metabolism/chemistry
Humans
*Epigenesis, Genetic
*Ovarian Neoplasms/microbiology
Fungi/metabolism
RevDate: 2024-10-25
CmpDate: 2024-10-25
A toxic relationship: ultra-processed foods & plastics.
Globalization and health, 20(1):74.
BACKGROUND: Among the crises engulfing the world is the symbiotic rise of ultra-processed foods (UPFs) and plastics. Together, this co-dependent duo generates substantial profits for agri-food and petrochemical industries at high costs for people and planet. Cheap, lightweight and highly functional, plastics have ideal properties that enable business models to create demand for low-cost, mass-produced and hyper-palatable UPFs among populations worldwide, hungry, or not. Evidence linking UPF consumption to deterioration in diet quality and higher risk of chronic diseases is well-established and growing rapidly. At the same time, the issue of plastic food contact chemicals (FCCs) is receiving increasing attention among the human health community, as is the generation and dispersion of micro- and nanoplastics.
MAIN BODY: In this commentary, we explore how the lifecycles and shared economic benefits of UPFs and plastics interact to co-produce a range of direct and indirect harms. We caution that the chemical dimension of these harms is underappreciated, with thousands of plastic FCCs known to migrate into foodstuffs. Some of these are hazardous and have been detected in humans and the broader environment, while many are yet to be adequately tested. We question whether policies on both UPF and plastic chemicals are fit for purpose when production and consumption of these products is adding to the chronic chemical exposures that plausibly contribute to the increasing global burden of non-communicable diseases.
CONCLUSIONS: In the context of ongoing negotiations for a legally binding global treaty to end plastics pollution, and rapidly growing concern about the burgeoning share of UPFs in diets worldwide, we ask: What steps are needed to call time on this toxic relationship?
Additional Links: PMID-39449069
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@article {pmid39449069,
year = {2024},
author = {Yates, J and Kadiyala, S and Deeney, M and Carriedo, A and Gillespie, S and Heindel, JJ and Maffini, MV and Martin, O and Monteiro, CA and Scheringer, M and Touvier, M and Muncke, J},
title = {A toxic relationship: ultra-processed foods & plastics.},
journal = {Globalization and health},
volume = {20},
number = {1},
pages = {74},
pmid = {39449069},
issn = {1744-8603},
mesh = {Humans ; *Plastics/adverse effects ; *Fast Foods/adverse effects ; Food Contamination ; Food, Processed ; },
abstract = {BACKGROUND: Among the crises engulfing the world is the symbiotic rise of ultra-processed foods (UPFs) and plastics. Together, this co-dependent duo generates substantial profits for agri-food and petrochemical industries at high costs for people and planet. Cheap, lightweight and highly functional, plastics have ideal properties that enable business models to create demand for low-cost, mass-produced and hyper-palatable UPFs among populations worldwide, hungry, or not. Evidence linking UPF consumption to deterioration in diet quality and higher risk of chronic diseases is well-established and growing rapidly. At the same time, the issue of plastic food contact chemicals (FCCs) is receiving increasing attention among the human health community, as is the generation and dispersion of micro- and nanoplastics.
MAIN BODY: In this commentary, we explore how the lifecycles and shared economic benefits of UPFs and plastics interact to co-produce a range of direct and indirect harms. We caution that the chemical dimension of these harms is underappreciated, with thousands of plastic FCCs known to migrate into foodstuffs. Some of these are hazardous and have been detected in humans and the broader environment, while many are yet to be adequately tested. We question whether policies on both UPF and plastic chemicals are fit for purpose when production and consumption of these products is adding to the chronic chemical exposures that plausibly contribute to the increasing global burden of non-communicable diseases.
CONCLUSIONS: In the context of ongoing negotiations for a legally binding global treaty to end plastics pollution, and rapidly growing concern about the burgeoning share of UPFs in diets worldwide, we ask: What steps are needed to call time on this toxic relationship?},
}
MeSH Terms:
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Humans
*Plastics/adverse effects
*Fast Foods/adverse effects
Food Contamination
Food, Processed
RevDate: 2024-10-24
Screening of Klebsiella pneumoniae isolates reveals the spread of strong biofilm formers and class 1 integrons.
Journal of applied microbiology pii:7840382 [Epub ahead of print].
BACKGROUND: Klebsiella pneumoniae is a Gram-negative bacterium that can colonize, penetrate, and cause infections at several human anatomical locations. The emergence of hypervirulent K. pneumoniae and its ability to evade the immune system and develop antibiotic resistance has made it a key concern in the healthcare industry. The hypervirulent variants are increasingly involved in community-acquired infections. Therefore, it is pertinent to understand the biofilm formation potential among the clinical isolates.
METHODS AND RESULTS: We acquired 225 isolates of K. pneumoniae from the Department of Microbiology, Symbiosis University Hospital and Research Centre (SUHRC), Pune, India over 1 year from March 2022- March 2023, and evaluated antimicrobial susceptibility, hypermucoviscous phenotype, virulence, and antimicrobial-resistant gene distribution in K. pneumoniae isolates and established a correlation between antimicrobial resistance and integrons. Most isolates were strong biofilm formers (76%). The isolates harbored one or more carbapenemase/ beta-lactamase encoding gene combinations. Hypermucoviscous (HMKP) isolates had considerably greater positive rates for iutA, magA, K2 serotype, rmpA, and rmpA2 than non-HMKP isolates. Isolates carrying integrons (43%) showed significantly more antibiotic resistance.
CONCLUSION: The study reveals spread of strong biofilm formers with extensive virulence and antimicrobial-resistant genes, and integrons responsible for multi-drug resistance among the clinical isolates of K. pneumoniae in Pune, India, posing a threat to the public health and necessitating close surveillance, accurate diagnosis, control, and therapeutic management of infections.
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PubMed:
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@article {pmid39448367,
year = {2024},
author = {Saha, U and Jadhav, SV and Pathak, KN and Saroj, SD},
title = {Screening of Klebsiella pneumoniae isolates reveals the spread of strong biofilm formers and class 1 integrons.},
journal = {Journal of applied microbiology},
volume = {},
number = {},
pages = {},
doi = {10.1093/jambio/lxae275},
pmid = {39448367},
issn = {1365-2672},
abstract = {BACKGROUND: Klebsiella pneumoniae is a Gram-negative bacterium that can colonize, penetrate, and cause infections at several human anatomical locations. The emergence of hypervirulent K. pneumoniae and its ability to evade the immune system and develop antibiotic resistance has made it a key concern in the healthcare industry. The hypervirulent variants are increasingly involved in community-acquired infections. Therefore, it is pertinent to understand the biofilm formation potential among the clinical isolates.
METHODS AND RESULTS: We acquired 225 isolates of K. pneumoniae from the Department of Microbiology, Symbiosis University Hospital and Research Centre (SUHRC), Pune, India over 1 year from March 2022- March 2023, and evaluated antimicrobial susceptibility, hypermucoviscous phenotype, virulence, and antimicrobial-resistant gene distribution in K. pneumoniae isolates and established a correlation between antimicrobial resistance and integrons. Most isolates were strong biofilm formers (76%). The isolates harbored one or more carbapenemase/ beta-lactamase encoding gene combinations. Hypermucoviscous (HMKP) isolates had considerably greater positive rates for iutA, magA, K2 serotype, rmpA, and rmpA2 than non-HMKP isolates. Isolates carrying integrons (43%) showed significantly more antibiotic resistance.
CONCLUSION: The study reveals spread of strong biofilm formers with extensive virulence and antimicrobial-resistant genes, and integrons responsible for multi-drug resistance among the clinical isolates of K. pneumoniae in Pune, India, posing a threat to the public health and necessitating close surveillance, accurate diagnosis, control, and therapeutic management of infections.},
}
RevDate: 2024-10-24
Exploring the role of the microbiome of the H. illucens (black soldier fly) for microbial synergy in optimizing black soldier fly rearing and subsequent applications.
Environmental pollution (Barking, Essex : 1987) pii:S0269-7491(24)01770-6 [Epub ahead of print].
The symbiotic microbiome in the insect's gut is vital to the host insect's development, improvement of health, resistance to disease, and adaptability to the environment. The black soldier fly (BSF) can convert organic substrates into a protein- and fat-rich biomass that is viable for various applications. With the support of a selective microbiome, BSF can digest and recycle different organic waste, reduce the harmful effects of improper disposal, and transform low-value side streams into valuable resources. Molecular and systems-level investigations on the harbored microbial populations may uncover new biocatalysts for organic waste degradation. This article discusses and summarizes the efforts taken toward characterizing the BSF microbiota and analyzing its substrate-dependent shifts. In addition, the review discusses the dynamic insect-microbe relationship from the functional point of view and focuses on how understanding this symbiosis can lead to alternative applications for BSF. Valorization strategies can include manipulating the microbiota to optimize insect growth and biomass production, as well as exploiting the role of BSF microbiota to discover new bioactive compounds based on BSF immunity. Optimizing the BSF application in industrial setup and exploiting its gut microbiota for innovative biotechnological applications are potential developments that could emerge in the coming decade.
Additional Links: PMID-39447631
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PubMed:
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@article {pmid39447631,
year = {2024},
author = {Salam, M and Bolletta, V and Meng, Y and Yakti, W and Grossule, V and Shi, D and Hayat, F},
title = {Exploring the role of the microbiome of the H. illucens (black soldier fly) for microbial synergy in optimizing black soldier fly rearing and subsequent applications.},
journal = {Environmental pollution (Barking, Essex : 1987)},
volume = {},
number = {},
pages = {125055},
doi = {10.1016/j.envpol.2024.125055},
pmid = {39447631},
issn = {1873-6424},
abstract = {The symbiotic microbiome in the insect's gut is vital to the host insect's development, improvement of health, resistance to disease, and adaptability to the environment. The black soldier fly (BSF) can convert organic substrates into a protein- and fat-rich biomass that is viable for various applications. With the support of a selective microbiome, BSF can digest and recycle different organic waste, reduce the harmful effects of improper disposal, and transform low-value side streams into valuable resources. Molecular and systems-level investigations on the harbored microbial populations may uncover new biocatalysts for organic waste degradation. This article discusses and summarizes the efforts taken toward characterizing the BSF microbiota and analyzing its substrate-dependent shifts. In addition, the review discusses the dynamic insect-microbe relationship from the functional point of view and focuses on how understanding this symbiosis can lead to alternative applications for BSF. Valorization strategies can include manipulating the microbiota to optimize insect growth and biomass production, as well as exploiting the role of BSF microbiota to discover new bioactive compounds based on BSF immunity. Optimizing the BSF application in industrial setup and exploiting its gut microbiota for innovative biotechnological applications are potential developments that could emerge in the coming decade.},
}
RevDate: 2024-10-24
Bioaccumulation of polycyclic aromatic hydrocarbons and microbiota dynamics across developmental stages of the Asian tiger mosquito, Aedes albopictus exposed to urban pollutants.
Ecotoxicology and environmental safety, 286:117214 pii:S0147-6513(24)01290-9 [Epub ahead of print].
Aedes albopictus mosquitoes face numerous anthropic stressors in urban areas. These xenobiotics not only impact mosquito physiology but also shape the composition of their microbiota, which play important roles in host physiological traits. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants known to alter mosquito metabolism, but no studies have yet investigated their impact on microbiota. Using a bespoke indoor mesocosm tailored for Ae. albopictus mosquitoes, we investigated the dynamics of bacterial communities in both mosquitoes and their larval breeding sites following chronic exposure to a cocktail of PAHs consisting of benzo[a]pyrene, benz[a]anthracene, chrysene and benzo[b]fluoranthene. Our findings showed that PAHs have a stage-specific effect on mosquito microbiota, with a higher impact in larvae than in adults, contributing to 12.5 % and 4.5 % of the PAHs-induced variations, respectively. The presence of PAHs in the treated mesocosm led to the enrichment of bacterial families and genera known for their ability to catabolize PAHs, such as Comamonadaceae and Raoultella (increasing from 19 % to 30 % and from 1.2 % to 5.6 %, respectively). Conversely, prevalent taxa found in mosquito microbiota like Wolbachia and Cedecea exhibited a reduction (decreasing from 4 % to 0.8 % and from 12.8 % to 6.4 %, respectively). This reduction could be attributed to the competitive advantage gained by PAH-degrading taxa, or it could reflect a direct sensitivity to PAH exposure. Overall, this indicates a shift in microbiota composition favoring bacteria that can thrive in a PAH-contaminated environment. PAHs persisted in the water of breeding sites only the first 45 days of the experiment. Benzo[a]pyrene and benzo[b]fluoranthene were more susceptible to bioaccumulation in larval tissues over time. Overall, this study enhances our understanding of the impact of pollution on mosquitoes and could facilitate future research on the importance of symbiosis in urban-dwelling insect disease vectors. Given the recent advancements in the generation of axenic (microbe-free) and gnotobiotic (mosquitoes with a defined or specific microbiota) mosquitoes, further studies are needed to explore how changes in microbiota composition could influence mosquito responses to pollution, particularly in relation to host fitness, immunity, and vector competence.
Additional Links: PMID-39447296
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PubMed:
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@article {pmid39447296,
year = {2024},
author = {Antonelli, P and Grizard, S and Tran, FH and Lejon, D and Bellemain, A and Van, and Mavingui, P and Roiz, D and Simard, F and Martin, E and Abrouk, D and Vigneron, A and Minard, G and Valiente Moro, C},
title = {Bioaccumulation of polycyclic aromatic hydrocarbons and microbiota dynamics across developmental stages of the Asian tiger mosquito, Aedes albopictus exposed to urban pollutants.},
journal = {Ecotoxicology and environmental safety},
volume = {286},
number = {},
pages = {117214},
doi = {10.1016/j.ecoenv.2024.117214},
pmid = {39447296},
issn = {1090-2414},
abstract = {Aedes albopictus mosquitoes face numerous anthropic stressors in urban areas. These xenobiotics not only impact mosquito physiology but also shape the composition of their microbiota, which play important roles in host physiological traits. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental pollutants known to alter mosquito metabolism, but no studies have yet investigated their impact on microbiota. Using a bespoke indoor mesocosm tailored for Ae. albopictus mosquitoes, we investigated the dynamics of bacterial communities in both mosquitoes and their larval breeding sites following chronic exposure to a cocktail of PAHs consisting of benzo[a]pyrene, benz[a]anthracene, chrysene and benzo[b]fluoranthene. Our findings showed that PAHs have a stage-specific effect on mosquito microbiota, with a higher impact in larvae than in adults, contributing to 12.5 % and 4.5 % of the PAHs-induced variations, respectively. The presence of PAHs in the treated mesocosm led to the enrichment of bacterial families and genera known for their ability to catabolize PAHs, such as Comamonadaceae and Raoultella (increasing from 19 % to 30 % and from 1.2 % to 5.6 %, respectively). Conversely, prevalent taxa found in mosquito microbiota like Wolbachia and Cedecea exhibited a reduction (decreasing from 4 % to 0.8 % and from 12.8 % to 6.4 %, respectively). This reduction could be attributed to the competitive advantage gained by PAH-degrading taxa, or it could reflect a direct sensitivity to PAH exposure. Overall, this indicates a shift in microbiota composition favoring bacteria that can thrive in a PAH-contaminated environment. PAHs persisted in the water of breeding sites only the first 45 days of the experiment. Benzo[a]pyrene and benzo[b]fluoranthene were more susceptible to bioaccumulation in larval tissues over time. Overall, this study enhances our understanding of the impact of pollution on mosquitoes and could facilitate future research on the importance of symbiosis in urban-dwelling insect disease vectors. Given the recent advancements in the generation of axenic (microbe-free) and gnotobiotic (mosquitoes with a defined or specific microbiota) mosquitoes, further studies are needed to explore how changes in microbiota composition could influence mosquito responses to pollution, particularly in relation to host fitness, immunity, and vector competence.},
}
RevDate: 2024-10-24
The evolution of thermal performance curves in fungi farmed by attine ant mutualists in aboveground or belowground microclimates.
Journal of evolutionary biology pii:7840318 [Epub ahead of print].
Fungi are abundant and ecologically important at a global scale, but little is known about whether their thermal adaptations are shaped by biochemical constraints (i.e. the Hotter is Better Model, HBM) or evolutionary tradeoffs (i.e., the Specialist Generalist Model, SGM). We tested these hypotheses by generating thermal performance curves (TPCs) of fungal cultivars farmed by six species of Panamanian fungus-farming 'attine' ants. These fungi represent evolutionary transitions in farming strategies as four cultivars are farmed by ants belowground at stable temperatures near 25°C and two cultivars are farmed aboveground at variable temperatures. We generated TPCs using a common garden experiment confining fungal isolates to different temperatures and then used a Bayesian hierarchical modeling approach to compare competing temperature sensitivity models. Some thermal performance traits differed consistently across farming strategies, with aboveground cultivars having: 1) higher tolerance to low temperatures (CTLmin) and 2) higher maximum growth rate at the optimal temperature (rmax). However, two core assumptions shared by the HBM or SGM were not supported as aboveground cultivars did not show systematic increases in either their optimal temperature (Topt) or thermal tolerance breadth. These results harness ant farming systems as long-term natural experiments to decouple the effects of environmental thermal variation and innate physiological temperature sensitivity on fungal thermal evolution. The results have clear implications for predicting climate warming induced breaking points in animal-microbe mutualisms.
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@article {pmid39447058,
year = {2024},
author = {Hess, O and van der Deure, T and Bolander, M and Leal Dutra, C and Shik, JZ},
title = {The evolution of thermal performance curves in fungi farmed by attine ant mutualists in aboveground or belowground microclimates.},
journal = {Journal of evolutionary biology},
volume = {},
number = {},
pages = {},
doi = {10.1093/jeb/voae135},
pmid = {39447058},
issn = {1420-9101},
abstract = {Fungi are abundant and ecologically important at a global scale, but little is known about whether their thermal adaptations are shaped by biochemical constraints (i.e. the Hotter is Better Model, HBM) or evolutionary tradeoffs (i.e., the Specialist Generalist Model, SGM). We tested these hypotheses by generating thermal performance curves (TPCs) of fungal cultivars farmed by six species of Panamanian fungus-farming 'attine' ants. These fungi represent evolutionary transitions in farming strategies as four cultivars are farmed by ants belowground at stable temperatures near 25°C and two cultivars are farmed aboveground at variable temperatures. We generated TPCs using a common garden experiment confining fungal isolates to different temperatures and then used a Bayesian hierarchical modeling approach to compare competing temperature sensitivity models. Some thermal performance traits differed consistently across farming strategies, with aboveground cultivars having: 1) higher tolerance to low temperatures (CTLmin) and 2) higher maximum growth rate at the optimal temperature (rmax). However, two core assumptions shared by the HBM or SGM were not supported as aboveground cultivars did not show systematic increases in either their optimal temperature (Topt) or thermal tolerance breadth. These results harness ant farming systems as long-term natural experiments to decouple the effects of environmental thermal variation and innate physiological temperature sensitivity on fungal thermal evolution. The results have clear implications for predicting climate warming induced breaking points in animal-microbe mutualisms.},
}
RevDate: 2024-10-24
CmpDate: 2024-10-24
Molecular identification and antimicrobial potential of endophytic fungi against some grapevine pathogens.
PloS one, 19(10):e0309041.
Endophytic fungi are microorganisms that, exhibiting within the plant tissues without causing any apparent harm to the host, establish a symbiotic relationship with plants. Host plants provide endophytic fungi with essential nutrients and a protected environment. In exchange, the fungi can enhance the plant's ability to acquire nutrients. They can also play a crucial role in increasing the host plant's tolerance to various abiotic and biotic stresses. Endophytic fungi can produce a wide range of bioactive compounds, some similar to those found in the host plant. In Iraq's Duhok province of the Kurdistan region, the plant species Vitis vinifera has been explored as a habitat for diverse endophytic microorganisms across various ecological environments. During the period from 2021 to 2022, a total of 600 samples were collected from four distinct locations: Bagera, Besfke, Barebhar, and Atrush. From these samples, twelve endophytic fungal species were isolated, including Aspergillus flavipes, Botryosphaeria dothidea, Fusarium oxysporum, Fusarium ruscicol, Fusarium venenatum, Chaetomium globosum, Clonostachys rosea, Mucor racemosus, Penicillium glabrum, Aspergillus terreus, Aspergillus nidulans, and Aspergillus niger, Alternaria alternata, Paecilomyces maximus, Curvularia buchloes. These fungi were introduced for their potential as biocontrol agents against grapevine trunk diseases and grape rotting fungi, which pose significant risks to grapevine health and productivity. Penicilium radiatolobatum, Botrysphaeria dothidea, Fusarium ruscicola, Fusarium venenatum, and Paecilomyces maximus represented the first record as endophytes on grapevine in Iraq. Based on ITS and SSU sequencing, molecular identification confirmed these fungi's presence with sequence identities ranging from 99% to 100%. Phylogenetic analysis revealed that these endophytes could be categorized into five main clusters (A, B, C, D, and E), showing high intra-group similarity. Utilizing the Dual Culture method, the endophyte Paecilomyces maximus demonstrated a 70.83% inhibition rate against Ilyonectria destructans. In the Food Poisoning method, A. flavipes and P. maximus emerged as the most effective inhibitors of Ilyonectria destructans, whereas A. terreus, M. racemosus, and P. maximus achieved complete inhibition (100%) of Botrytis cinerea. Additionally, M. racemosus was identified as the most effective biocontrol agent against Neoscytalidium dimidiatum. In conclusion, the study emphasizes the potential of endophytic fungi from Vitis vinifera as effective biocontrol agents against grapevine diseases, highlighting their role in sustainable vineyard management. These findings lead to further exploration and implementation of these fungi-inserted pest management strategies.
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Citation:
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@article {pmid39446969,
year = {2024},
author = {Nashat, LH and Haleem, RA and Ali, SH},
title = {Molecular identification and antimicrobial potential of endophytic fungi against some grapevine pathogens.},
journal = {PloS one},
volume = {19},
number = {10},
pages = {e0309041},
pmid = {39446969},
issn = {1932-6203},
mesh = {*Vitis/microbiology ; *Endophytes/physiology ; *Fungi ; Plant Diseases/microbiology/prevention & control ; Fusarium/physiology ; },
abstract = {Endophytic fungi are microorganisms that, exhibiting within the plant tissues without causing any apparent harm to the host, establish a symbiotic relationship with plants. Host plants provide endophytic fungi with essential nutrients and a protected environment. In exchange, the fungi can enhance the plant's ability to acquire nutrients. They can also play a crucial role in increasing the host plant's tolerance to various abiotic and biotic stresses. Endophytic fungi can produce a wide range of bioactive compounds, some similar to those found in the host plant. In Iraq's Duhok province of the Kurdistan region, the plant species Vitis vinifera has been explored as a habitat for diverse endophytic microorganisms across various ecological environments. During the period from 2021 to 2022, a total of 600 samples were collected from four distinct locations: Bagera, Besfke, Barebhar, and Atrush. From these samples, twelve endophytic fungal species were isolated, including Aspergillus flavipes, Botryosphaeria dothidea, Fusarium oxysporum, Fusarium ruscicol, Fusarium venenatum, Chaetomium globosum, Clonostachys rosea, Mucor racemosus, Penicillium glabrum, Aspergillus terreus, Aspergillus nidulans, and Aspergillus niger, Alternaria alternata, Paecilomyces maximus, Curvularia buchloes. These fungi were introduced for their potential as biocontrol agents against grapevine trunk diseases and grape rotting fungi, which pose significant risks to grapevine health and productivity. Penicilium radiatolobatum, Botrysphaeria dothidea, Fusarium ruscicola, Fusarium venenatum, and Paecilomyces maximus represented the first record as endophytes on grapevine in Iraq. Based on ITS and SSU sequencing, molecular identification confirmed these fungi's presence with sequence identities ranging from 99% to 100%. Phylogenetic analysis revealed that these endophytes could be categorized into five main clusters (A, B, C, D, and E), showing high intra-group similarity. Utilizing the Dual Culture method, the endophyte Paecilomyces maximus demonstrated a 70.83% inhibition rate against Ilyonectria destructans. In the Food Poisoning method, A. flavipes and P. maximus emerged as the most effective inhibitors of Ilyonectria destructans, whereas A. terreus, M. racemosus, and P. maximus achieved complete inhibition (100%) of Botrytis cinerea. Additionally, M. racemosus was identified as the most effective biocontrol agent against Neoscytalidium dimidiatum. In conclusion, the study emphasizes the potential of endophytic fungi from Vitis vinifera as effective biocontrol agents against grapevine diseases, highlighting their role in sustainable vineyard management. These findings lead to further exploration and implementation of these fungi-inserted pest management strategies.},
}
MeSH Terms:
show MeSH Terms
hide MeSH Terms
*Vitis/microbiology
*Endophytes/physiology
*Fungi
Plant Diseases/microbiology/prevention & control
Fusarium/physiology
RevDate: 2024-10-24
Mini review: antimicrobial compounds produced by bacteria associated with marine invertebrates.
Folia microbiologica [Epub ahead of print].
The marine environment is considered one of the most important ecosystems with high biodiversity. Microorganisms in this environment are variable and coexist with other marine organisms. The microbes associated with other marine organisms produce compounds with biological activity that may help the host's defense against invading organisms. The symbiotic association of bacteria with marine invertebrates is of ecological and biotechnological importance. Biologically active metabolites isolated from bacteria associated with marine invertebrates are considered potential sources of natural antimicrobial molecules for treating infectious diseases. Many studies have been conducted to screen the antimicrobial activity of metabolites produced by bacteria associated with marine invertebrates. This work provides an overview of the advancements in antimicrobial compound research on bacteria associated with marine invertebrates.
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@article {pmid39446239,
year = {2024},
author = {Amran, RH and Jamal, MT and Bowrji, S and Sayegh, F and Santanumurti, MB and Satheesh, S},
title = {Mini review: antimicrobial compounds produced by bacteria associated with marine invertebrates.},
journal = {Folia microbiologica},
volume = {},
number = {},
pages = {},
pmid = {39446239},
issn = {1874-9356},
abstract = {The marine environment is considered one of the most important ecosystems with high biodiversity. Microorganisms in this environment are variable and coexist with other marine organisms. The microbes associated with other marine organisms produce compounds with biological activity that may help the host's defense against invading organisms. The symbiotic association of bacteria with marine invertebrates is of ecological and biotechnological importance. Biologically active metabolites isolated from bacteria associated with marine invertebrates are considered potential sources of natural antimicrobial molecules for treating infectious diseases. Many studies have been conducted to screen the antimicrobial activity of metabolites produced by bacteria associated with marine invertebrates. This work provides an overview of the advancements in antimicrobial compound research on bacteria associated with marine invertebrates.},
}
RevDate: 2024-10-24
Genome sequence of the marine alphaproteobacterium Lentilitoribacter sp. EG35 isolated from the temperate octocoral Eunicella gazella.
Microbiology resource announcements [Epub ahead of print].
We report the genome sequence of Lentilitoribacter sp. strain EG35 isolated from the octocoral Eunicella gazella sampled off the coast of Portugal. We reveal the coding potential for the biosynthesis of polyhydroxyalkanoates - biodegradable polyesters that may serve bioplastics production, diverse homoserine lactone-like communication signals, and four putatively novel natural products.
Additional Links: PMID-39445820
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PubMed:
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@article {pmid39445820,
year = {2024},
author = {Keller-Costa, T and Madureira, S and Fernandes, AS and Kozma, L and Gonçalves, JM and Barroso, C and Egas, C and Costa, R},
title = {Genome sequence of the marine alphaproteobacterium Lentilitoribacter sp. EG35 isolated from the temperate octocoral Eunicella gazella.},
journal = {Microbiology resource announcements},
volume = {},
number = {},
pages = {e0087224},
doi = {10.1128/mra.00872-24},
pmid = {39445820},
issn = {2576-098X},
abstract = {We report the genome sequence of Lentilitoribacter sp. strain EG35 isolated from the octocoral Eunicella gazella sampled off the coast of Portugal. We reveal the coding potential for the biosynthesis of polyhydroxyalkanoates - biodegradable polyesters that may serve bioplastics production, diverse homoserine lactone-like communication signals, and four putatively novel natural products.},
}
RevDate: 2024-10-25
CmpDate: 2024-10-24
Microbiota activation and regulation of adaptive immunity.
Frontiers in immunology, 15:1429436.
In the mucosa, T cells and B cells of the immune system are essential for maintaining immune homeostasis by suppressing reactions to harmless antigens and upholding the integrity of intestinal mucosal barrier functions. Host immunity and homeostasis are regulated by metabolites produced by the gut microbiota, which has developed through the long-term coevolution of the host and the gut biome. This is achieved by the immunological system's tolerance for symbiote microbiota, and its ability to generate a proinflammatory response against invasive organisms. The imbalance of the intestinal immune system with commensal organisms is causing a disturbance in the homeostasis of the gut microbiome. The lack of balance results in microbiota dysbiosis, the weakened integrity of the gut barrier, and the development of inflammatory immune reactions toward symbiotic organisms. Researchers may uncover potential therapeutic targets for preventing or regulating inflammatory diseases by understanding the interactions between adaptive immunity and the microbiota. This discussion will explore the connection between adaptive immunity and microbiota.
Additional Links: PMID-39445008
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@article {pmid39445008,
year = {2024},
author = {Heidari, M and Maleki Vareki, S and Yaghobi, R and Karimi, MH},
title = {Microbiota activation and regulation of adaptive immunity.},
journal = {Frontiers in immunology},
volume = {15},
number = {},
pages = {1429436},
pmid = {39445008},
issn = {1664-3224},
mesh = {*Adaptive Immunity ; Humans ; Animals ; *Gastrointestinal Microbiome/immunology ; Homeostasis/immunology ; Dysbiosis/immunology ; Intestinal Mucosa/immunology/microbiology ; Symbiosis/immunology ; },
abstract = {In the mucosa, T cells and B cells of the immune system are essential for maintaining immune homeostasis by suppressing reactions to harmless antigens and upholding the integrity of intestinal mucosal barrier functions. Host immunity and homeostasis are regulated by metabolites produced by the gut microbiota, which has developed through the long-term coevolution of the host and the gut biome. This is achieved by the immunological system's tolerance for symbiote microbiota, and its ability to generate a proinflammatory response against invasive organisms. The imbalance of the intestinal immune system with commensal organisms is causing a disturbance in the homeostasis of the gut microbiome. The lack of balance results in microbiota dysbiosis, the weakened integrity of the gut barrier, and the development of inflammatory immune reactions toward symbiotic organisms. Researchers may uncover potential therapeutic targets for preventing or regulating inflammatory diseases by understanding the interactions between adaptive immunity and the microbiota. This discussion will explore the connection between adaptive immunity and microbiota.},
}
MeSH Terms:
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*Adaptive Immunity
Humans
Animals
*Gastrointestinal Microbiome/immunology
Homeostasis/immunology
Dysbiosis/immunology
Intestinal Mucosa/immunology/microbiology
Symbiosis/immunology
RevDate: 2024-10-25
Comparative metagenomic study unveils new insights on bacterial communities in two pine-feeding Ips beetles (Coleoptera: Curculionidae: Scolytinae).
Frontiers in microbiology, 15:1400894.
BACKGROUND: Climate change has recently boosted the severity and frequency of pine bark beetle attacks. The bacterial community associated with these beetles acts as "hidden players," enhancing their ability to infest and thrive on defense-rich pine trees. There is limited understanding of the environmental acquisition of these hidden players and their life stage-specific association with different pine-feeding bark beetles. There is inadequate knowledge on novel bacterial introduction to pine trees after the beetle infestation. Hence, we conducted the first comparative bacterial metabarcoding study revealing the bacterial communities in the pine trees before and after beetle feeding and in different life stages of two dominant pine-feeding bark beetles, namely Ips sexdentatus and Ips acuminatus. We also evaluated the bacterial association between wild and lab-bred beetles to measure the deviation due to inhabiting a controlled environment.
RESULTS: Significant differences in bacterial amplicon sequence variance (ASVs) abundance existed among different life stages within and between the pine beetles. However, Pseudomonas, Serratia, Pseudoxanthomonas, Taibaiella, and Acinetobacter served as core bacteria. Interestingly, I. sexdentatus larvae correspond to significantly higher bacterial diversity and community richness and evenness compared to other developmental stages, while I. acuminatus adults displayed higher bacterial richness with no significant variation in the diversity and evenness between the life stages. Both wild and lab-bred I. sexdentatus beetles showed a prevalence of the bacterial family Pseudomonadaceae. In addition, wild I. sexdentatus showed dominance of Yersiniaceae, whereas Erwiniaceae was abundant in lab-bred beetles. Alternatively, Acidobacteriaceae, Corynebacteriaceae, and Microbacteriaceae were highly abundant bacterial families in lab-bred, whereas Chitinophagaceae and Microbacteriaceae were highly abundant in wild I. accuminatus. We validated the relative abundances of selected bacterial taxa estimated by metagenomic sequencing with quantitative PCR.
CONCLUSION: Our study sheds new insights into bacterial associations in pine beetles under the influence of various drivers such as environment, host, and life stages. We documented that lab-breeding considerably influences beetle bacterial community assembly. Furthermore, beetle feeding alters bacteriome at the microhabitat level. Nevertheless, our study revisited pine-feeding bark beetle symbiosis under the influence of different drivers and revealed intriguing insight into bacterial community assembly, facilitating future functional studies.
Additional Links: PMID-39444680
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Citation:
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@article {pmid39444680,
year = {2024},
author = {Khara, A and Chakraborty, A and Modlinger, R and Synek, J and Roy, A},
title = {Comparative metagenomic study unveils new insights on bacterial communities in two pine-feeding Ips beetles (Coleoptera: Curculionidae: Scolytinae).},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1400894},
pmid = {39444680},
issn = {1664-302X},
abstract = {BACKGROUND: Climate change has recently boosted the severity and frequency of pine bark beetle attacks. The bacterial community associated with these beetles acts as "hidden players," enhancing their ability to infest and thrive on defense-rich pine trees. There is limited understanding of the environmental acquisition of these hidden players and their life stage-specific association with different pine-feeding bark beetles. There is inadequate knowledge on novel bacterial introduction to pine trees after the beetle infestation. Hence, we conducted the first comparative bacterial metabarcoding study revealing the bacterial communities in the pine trees before and after beetle feeding and in different life stages of two dominant pine-feeding bark beetles, namely Ips sexdentatus and Ips acuminatus. We also evaluated the bacterial association between wild and lab-bred beetles to measure the deviation due to inhabiting a controlled environment.
RESULTS: Significant differences in bacterial amplicon sequence variance (ASVs) abundance existed among different life stages within and between the pine beetles. However, Pseudomonas, Serratia, Pseudoxanthomonas, Taibaiella, and Acinetobacter served as core bacteria. Interestingly, I. sexdentatus larvae correspond to significantly higher bacterial diversity and community richness and evenness compared to other developmental stages, while I. acuminatus adults displayed higher bacterial richness with no significant variation in the diversity and evenness between the life stages. Both wild and lab-bred I. sexdentatus beetles showed a prevalence of the bacterial family Pseudomonadaceae. In addition, wild I. sexdentatus showed dominance of Yersiniaceae, whereas Erwiniaceae was abundant in lab-bred beetles. Alternatively, Acidobacteriaceae, Corynebacteriaceae, and Microbacteriaceae were highly abundant bacterial families in lab-bred, whereas Chitinophagaceae and Microbacteriaceae were highly abundant in wild I. accuminatus. We validated the relative abundances of selected bacterial taxa estimated by metagenomic sequencing with quantitative PCR.
CONCLUSION: Our study sheds new insights into bacterial associations in pine beetles under the influence of various drivers such as environment, host, and life stages. We documented that lab-breeding considerably influences beetle bacterial community assembly. Furthermore, beetle feeding alters bacteriome at the microhabitat level. Nevertheless, our study revisited pine-feeding bark beetle symbiosis under the influence of different drivers and revealed intriguing insight into bacterial community assembly, facilitating future functional studies.},
}
RevDate: 2024-10-24
Coral photosymbiosis on Mid-Devonian reefs.
Nature [Epub ahead of print].
The ability of stony corals to thrive in the oligotrophic (low-nutrient, low-productivity) surface waters of the tropical ocean is commonly attributed to their symbiotic relationship with photosynthetic dinoflagellates[1,2]. The evolutionary history of this symbiosis might clarify its organismal and environmental roles[3], but its prevalence through time, and across taxa, morphologies and oceanic settings, is currently unclear[4-6]. Here we report measurements of the nitrogen isotope ([15]N/[14]N) ratio of coral-bound organic matter (CB-δ[15]N) in samples from Mid-Devonian reefs (Givetian, around 385 million years ago), which represent a constraint on the evolution of coral photosymbiosis. Colonial tabulate and fasciculate (dendroid) rugose corals have low CB-δ[15]N values (2.51 ± 0.97‰) in comparison with co-occurring solitary and (pseudo)colonial (cerioid or phaceloid) rugose corals (5.52 ± 1.63‰). The average of the isotopic difference per deposit (3.01 ± 0.58‰) is statistically indistinguishable from that observed between modern symbiont-barren and symbiont-bearing corals (3.38 ± 1.05‰). On the basis of this evidence, we infer that Mid-Devonian tabulate and some fasciculate (dendroid) rugose corals hosted active photosymbionts, while solitary and some (pseudo)colonial (cerioid or phaceloid) rugose corals did not. The low CB-δ[15]N values of the Devonian tabulate and fasciculate rugose corals relative to the modern range suggest that Mid-Devonian reefs formed in biogeochemical regimes analogous to the modern oligotrophic subtropical gyres. Widespread oligotrophy during the Devonian may have promoted coral photosymbiosis, the occurrence of which may explain why Devonian reefs were the most productive reef ecosystems of the Phanerozoic.
Additional Links: PMID-39443794
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Citation:
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@article {pmid39443794,
year = {2024},
author = {Jung, J and Zoppe, SF and Söte, T and Moretti, S and Duprey, NN and Foreman, AD and Wald, T and Vonhof, H and Haug, GH and Sigman, DM and Mulch, A and Schindler, E and Janussen, D and Martínez-García, A},
title = {Coral photosymbiosis on Mid-Devonian reefs.},
journal = {Nature},
volume = {},
number = {},
pages = {},
pmid = {39443794},
issn = {1476-4687},
abstract = {The ability of stony corals to thrive in the oligotrophic (low-nutrient, low-productivity) surface waters of the tropical ocean is commonly attributed to their symbiotic relationship with photosynthetic dinoflagellates[1,2]. The evolutionary history of this symbiosis might clarify its organismal and environmental roles[3], but its prevalence through time, and across taxa, morphologies and oceanic settings, is currently unclear[4-6]. Here we report measurements of the nitrogen isotope ([15]N/[14]N) ratio of coral-bound organic matter (CB-δ[15]N) in samples from Mid-Devonian reefs (Givetian, around 385 million years ago), which represent a constraint on the evolution of coral photosymbiosis. Colonial tabulate and fasciculate (dendroid) rugose corals have low CB-δ[15]N values (2.51 ± 0.97‰) in comparison with co-occurring solitary and (pseudo)colonial (cerioid or phaceloid) rugose corals (5.52 ± 1.63‰). The average of the isotopic difference per deposit (3.01 ± 0.58‰) is statistically indistinguishable from that observed between modern symbiont-barren and symbiont-bearing corals (3.38 ± 1.05‰). On the basis of this evidence, we infer that Mid-Devonian tabulate and some fasciculate (dendroid) rugose corals hosted active photosymbionts, while solitary and some (pseudo)colonial (cerioid or phaceloid) rugose corals did not. The low CB-δ[15]N values of the Devonian tabulate and fasciculate rugose corals relative to the modern range suggest that Mid-Devonian reefs formed in biogeochemical regimes analogous to the modern oligotrophic subtropical gyres. Widespread oligotrophy during the Devonian may have promoted coral photosymbiosis, the occurrence of which may explain why Devonian reefs were the most productive reef ecosystems of the Phanerozoic.},
}
RevDate: 2024-10-23
Revealing the Interplay of Dissolved Organic Matters Variation with Microbial Symbiotic Network in Lime-Treated Sludge Landscaping.
Environmental research pii:S0013-9351(24)02123-6 [Epub ahead of print].
Lime pretreatment is commonly used for sludge hygienization. Appropriate lime dosage is crucial for achieving both sludge stabilization (lime dosage > 0.2 g/g-TS) and promoting plant and soil health during subsequent landscaping (lime dosage < 0.8 g/g-TS). While much research has been conducted on sludge lime treatment, few studies have examined the effects of lime dosing on integrating sludge stabilization and plant growth promotion during landscaping. In this study, we investigated microbial dynamics and dissolved organic matter (DOM) transformation during sludge landscaping with five lime dosage gradients (0, 0.2, 0.4, 0.6, 0.8 g lime/g-TS) over 90 days. Our results showed that a lime dosage of 0.4 g/g-TS is the lower threshold for achieving waste activated sludge (WAS) stabilization during landscaping, leading to maximum humic substance formation and minimal phytotoxicity. Specifically, at 0.4 g/g-TS lime dosage, protein degradation and decarboxylation-induced humification were significantly enhanced. The predominant microbial genera shifted from Aromatoleum to Exiguobacterium and Romboutsia (both affiliated with the phylum Firmicutes). Reactomics analysis further indicated that a 0.4 g/g-TS lime dosage promoted the hydrolysis of proteins (lyase reactions on C-C, C-O, and C-N bonds), amino acid metabolism, and decarboxylation-induced humification (e.g., C1H2O2, C2H4O2, C5H4O2, C6H4O2). The co-occurrence network analysis suggested that the phyla Firmicutes, Proteobacteria, and Bacteroidetes were key players in DOM transformation. This study provides an in-depth understanding of microbe-mediated DOM transformation during sludge landscaping and identifies the optimal lime dosage for improving sludge landscaping efficiency.
Additional Links: PMID-39442659
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PubMed:
Citation:
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@article {pmid39442659,
year = {2024},
author = {Chen, Z and Zhang, Y and Yang, B and Fan, S and Li, L and Yang, P and Zhang, W},
title = {Revealing the Interplay of Dissolved Organic Matters Variation with Microbial Symbiotic Network in Lime-Treated Sludge Landscaping.},
journal = {Environmental research},
volume = {},
number = {},
pages = {120216},
doi = {10.1016/j.envres.2024.120216},
pmid = {39442659},
issn = {1096-0953},
abstract = {Lime pretreatment is commonly used for sludge hygienization. Appropriate lime dosage is crucial for achieving both sludge stabilization (lime dosage > 0.2 g/g-TS) and promoting plant and soil health during subsequent landscaping (lime dosage < 0.8 g/g-TS). While much research has been conducted on sludge lime treatment, few studies have examined the effects of lime dosing on integrating sludge stabilization and plant growth promotion during landscaping. In this study, we investigated microbial dynamics and dissolved organic matter (DOM) transformation during sludge landscaping with five lime dosage gradients (0, 0.2, 0.4, 0.6, 0.8 g lime/g-TS) over 90 days. Our results showed that a lime dosage of 0.4 g/g-TS is the lower threshold for achieving waste activated sludge (WAS) stabilization during landscaping, leading to maximum humic substance formation and minimal phytotoxicity. Specifically, at 0.4 g/g-TS lime dosage, protein degradation and decarboxylation-induced humification were significantly enhanced. The predominant microbial genera shifted from Aromatoleum to Exiguobacterium and Romboutsia (both affiliated with the phylum Firmicutes). Reactomics analysis further indicated that a 0.4 g/g-TS lime dosage promoted the hydrolysis of proteins (lyase reactions on C-C, C-O, and C-N bonds), amino acid metabolism, and decarboxylation-induced humification (e.g., C1H2O2, C2H4O2, C5H4O2, C6H4O2). The co-occurrence network analysis suggested that the phyla Firmicutes, Proteobacteria, and Bacteroidetes were key players in DOM transformation. This study provides an in-depth understanding of microbe-mediated DOM transformation during sludge landscaping and identifies the optimal lime dosage for improving sludge landscaping efficiency.},
}
RevDate: 2024-10-23
Repeated horizontal acquisition of lagriamide-producing symbionts in Lagriinae beetles.
The ISME journal pii:7833433 [Epub ahead of print].
Microbial symbionts associate with multicellular organisms on a continuum from facultative associations to mutual codependency. In the oldest intracellular symbioses there is exclusive vertical symbiont transmission, and co-diversification of symbiotic partners over millions of years. Such symbionts often undergo genome reduction due to low effective population sizes, frequent population bottlenecks, and reduced purifying selection. Here, we describe multiple independent acquisition events of closely related defensive symbionts followed by genome erosion in a group of Lagriinae beetles. Previous work in Lagria villosa revealed the dominant genome-eroded symbiont of the genus Burkholderia produces the antifungal compound lagriamide, protecting the beetle's eggs and larvae from antagonistic fungi. Here, we use metagenomics to assemble 11 additional genomes of lagriamide-producing symbionts from seven different host species within Lagriinae from five countries, to unravel the evolutionary history of this symbiotic relationship. In each host, we detected one dominant genome-eroded Burkholderia symbiont encoding the lagriamide biosynthetic gene cluster. However, we did not find evidence for host-symbiont co-diversification, or for monophyly of the lagriamide-producing symbionts. Instead, our analyses support a single ancestral acquisition of the gene cluster followed by at least four independent symbiont acquisitions and subsequent genome erosion in each lineage. By contrast, a clade of plant-associated relatives retained large genomes but secondarily lost the lagriamide gene cluster. Our results, therefore, reveal a dynamic evolutionary history with multiple independent symbiont acquisitions characterized by a high degree of specificity, and highlight the importance of the specialized metabolite lagriamide for the establishment and maintenance of this defensive symbiosis.
Additional Links: PMID-39441990
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@article {pmid39441990,
year = {2024},
author = {Uppal, S and Waterworth, SC and Nick, A and Vogel, H and Flórez, LV and Kaltenpoth, M and Kwan, JC},
title = {Repeated horizontal acquisition of lagriamide-producing symbionts in Lagriinae beetles.},
journal = {The ISME journal},
volume = {},
number = {},
pages = {},
doi = {10.1093/ismejo/wrae211},
pmid = {39441990},
issn = {1751-7370},
abstract = {Microbial symbionts associate with multicellular organisms on a continuum from facultative associations to mutual codependency. In the oldest intracellular symbioses there is exclusive vertical symbiont transmission, and co-diversification of symbiotic partners over millions of years. Such symbionts often undergo genome reduction due to low effective population sizes, frequent population bottlenecks, and reduced purifying selection. Here, we describe multiple independent acquisition events of closely related defensive symbionts followed by genome erosion in a group of Lagriinae beetles. Previous work in Lagria villosa revealed the dominant genome-eroded symbiont of the genus Burkholderia produces the antifungal compound lagriamide, protecting the beetle's eggs and larvae from antagonistic fungi. Here, we use metagenomics to assemble 11 additional genomes of lagriamide-producing symbionts from seven different host species within Lagriinae from five countries, to unravel the evolutionary history of this symbiotic relationship. In each host, we detected one dominant genome-eroded Burkholderia symbiont encoding the lagriamide biosynthetic gene cluster. However, we did not find evidence for host-symbiont co-diversification, or for monophyly of the lagriamide-producing symbionts. Instead, our analyses support a single ancestral acquisition of the gene cluster followed by at least four independent symbiont acquisitions and subsequent genome erosion in each lineage. By contrast, a clade of plant-associated relatives retained large genomes but secondarily lost the lagriamide gene cluster. Our results, therefore, reveal a dynamic evolutionary history with multiple independent symbiont acquisitions characterized by a high degree of specificity, and highlight the importance of the specialized metabolite lagriamide for the establishment and maintenance of this defensive symbiosis.},
}
RevDate: 2024-10-23
Alteration of Cecal Microbiota by Antimicrobial Peptides Enhances the Rational and Efficient Utilization of Nutrients in Holstein Bulls.
Probiotics and antimicrobial proteins [Epub ahead of print].
We previously observed that supplementation with antimicrobial peptides facilitated the average daily weight gain, net meat, and carcass weights of Holstein bulls. To expand our knowledge of the possible impact of antimicrobial peptides on cecum microbiota, further investigations were conducted. In this study, 18 castrated Holstein bulls with insignificant weight differences and 10 months of age were split randomly into two groups. The control group (CK) was fed a basic diet, whereas the antimicrobial peptide group (AP) was supplemented with 8 g of antimicrobial peptides for 270 days. After slaughter, metagenomic and metabolomic sequencing analyses were performed on the cecum contents. The results showed significantly higher levels of amylase, cellulase, protease, and lipase in the CK than in the AP group (P ≤ 0.05). The levels of β-glucosidase and xylanase (P ≤ 0.05), and acetic and propionic acids (P ≤ 0.01), were considerably elevated in the AP than in the CK group. The metagenome showed variations between the two groups only at the bacterial level, and 3258 bacteria with differences were annotated. A total of 138 differential abundant genes (P < 0.05) were identified in the CAZyme map, with 65 genes more abundant in the cecum of the AP group and 48 genes more abundant in the cecum of the CK group. Metabolomic analysis identified 68 differentially expressed metabolites. Conjoint analysis of microorganisms and metabolites revealed that Lactobacillus had the greatest impact on metabolites in the AP group and Brumimicrobium in the CK group. The advantageous strains of the AP group Firmicutes bacterium CAG:110 exhibited a strong symbiotic relationship with urodeoxycholic acid and hyodeoxycholic acid. This study identified the classification characteristics, functions, metabolites, and interactions of cecal microbiota with metabolites that contribute to host growth performance. Antimicrobial peptides affect the cecal microorganisms, making the use of nutrients more efficient. The utilization of hemicellulose in the cecum of ruminants may contribute more than cellulose to their production performance.
Additional Links: PMID-39441337
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@article {pmid39441337,
year = {2024},
author = {Shi, J and Lei, Y and Li, Z and Jia, L and He, P and Cheng, Q and Zhang, Z and Lei, Z},
title = {Alteration of Cecal Microbiota by Antimicrobial Peptides Enhances the Rational and Efficient Utilization of Nutrients in Holstein Bulls.},
journal = {Probiotics and antimicrobial proteins},
volume = {},
number = {},
pages = {},
pmid = {39441337},
issn = {1867-1314},
support = {GSA-XMLZ-2021-01//Gansu beef cattle quality fattening project/ ; GSSLCSX-2020-1//the local funding/ ; },
abstract = {We previously observed that supplementation with antimicrobial peptides facilitated the average daily weight gain, net meat, and carcass weights of Holstein bulls. To expand our knowledge of the possible impact of antimicrobial peptides on cecum microbiota, further investigations were conducted. In this study, 18 castrated Holstein bulls with insignificant weight differences and 10 months of age were split randomly into two groups. The control group (CK) was fed a basic diet, whereas the antimicrobial peptide group (AP) was supplemented with 8 g of antimicrobial peptides for 270 days. After slaughter, metagenomic and metabolomic sequencing analyses were performed on the cecum contents. The results showed significantly higher levels of amylase, cellulase, protease, and lipase in the CK than in the AP group (P ≤ 0.05). The levels of β-glucosidase and xylanase (P ≤ 0.05), and acetic and propionic acids (P ≤ 0.01), were considerably elevated in the AP than in the CK group. The metagenome showed variations between the two groups only at the bacterial level, and 3258 bacteria with differences were annotated. A total of 138 differential abundant genes (P < 0.05) were identified in the CAZyme map, with 65 genes more abundant in the cecum of the AP group and 48 genes more abundant in the cecum of the CK group. Metabolomic analysis identified 68 differentially expressed metabolites. Conjoint analysis of microorganisms and metabolites revealed that Lactobacillus had the greatest impact on metabolites in the AP group and Brumimicrobium in the CK group. The advantageous strains of the AP group Firmicutes bacterium CAG:110 exhibited a strong symbiotic relationship with urodeoxycholic acid and hyodeoxycholic acid. This study identified the classification characteristics, functions, metabolites, and interactions of cecal microbiota with metabolites that contribute to host growth performance. Antimicrobial peptides affect the cecal microorganisms, making the use of nutrients more efficient. The utilization of hemicellulose in the cecum of ruminants may contribute more than cellulose to their production performance.},
}
RevDate: 2024-10-23
CmpDate: 2024-10-23
THE INFLUENCE OF THE DEMODEX MITE ON THE MORPHOLOGICAL PICTURE OF EYELID PAPILLOMA.
Georgian medical news.
UNLABELLED: The aim of work is description of new observations related to the participation of demodex in tumor morphogenesis with goal to study the example of eyelid papilloma, imagine the connection between the presence of the demodex mite and certain changes in the typical histomorphological picture of the neoplasm.
MATERIALS AND METHODS: Histomorphological examination covers biopsy and operative material of eye pathology with diagnosis of neoplasm (eyelid papilloma and actinic keratosis (senile keratosis, senile keratoma, solar keratosis)) were selected, with special attention paid to the presence of horn cysts. Agile methodologies were employed to manage the research workflow effectively.
RESULTS: As a result of the conducted histomorphological examination, in 24 cases (48%), patterns pathognomonic for demodectic infection were found. Such patterns, which could indicate the presence of a demodex mite, were cystic formations, as well as fragments of a dead parasite. We noted their presence not only in the tumor tissue, but also in the adjacent hair follicles, sebaceous glands, and subepidermal stroma. It is no coincidence that the authors who previously noted the presence of pigmented elements in keratopapillomas or seborrheic keratomas could not explain their origin, resorting to putting forward such fantastic assumptions as the presence of "symbiosis of melanoblasts and epithelial cells, which ensures the transfer of pigment from the first to the second".
CONCLUSIONS: Histomorphological study of serial sections allows us to see the successive stages of transformation of cavity intratissue defects created by the activity of the mite into horn cysts. Cavity defects are the main evidence of demodectic infestation. They occur in basal cell and squamous cell carcinomas, xanthomas, sebaceous adenomas, etc. tumors of the eyelids, but only in keratoacanthomas and senile keratomas they can turn into corneal cysts, which can be assumed to be due to the differentiating potential of these neoplasms.
Additional Links: PMID-39441269
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Citation:
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@article {pmid39441269,
year = {2024},
author = {Artemov, O and Lytvynenko, M and Chumachenko, I and Bondarenko, A and Dotsenko, N and Ostapchuk, K and Koshelnyk, O and Gargin, V},
title = {THE INFLUENCE OF THE DEMODEX MITE ON THE MORPHOLOGICAL PICTURE OF EYELID PAPILLOMA.},
journal = {Georgian medical news},
volume = {},
number = {352-353},
pages = {50-54},
pmid = {39441269},
issn = {1512-0112},
mesh = {Humans ; Animals ; *Papilloma/pathology/parasitology/diagnostic imaging ; *Mites ; Eyelid Neoplasms/pathology/parasitology/diagnostic imaging ; Male ; Female ; Mite Infestations/pathology/parasitology ; Aged ; Keratosis, Actinic/pathology ; Middle Aged ; },
abstract = {UNLABELLED: The aim of work is description of new observations related to the participation of demodex in tumor morphogenesis with goal to study the example of eyelid papilloma, imagine the connection between the presence of the demodex mite and certain changes in the typical histomorphological picture of the neoplasm.
MATERIALS AND METHODS: Histomorphological examination covers biopsy and operative material of eye pathology with diagnosis of neoplasm (eyelid papilloma and actinic keratosis (senile keratosis, senile keratoma, solar keratosis)) were selected, with special attention paid to the presence of horn cysts. Agile methodologies were employed to manage the research workflow effectively.
RESULTS: As a result of the conducted histomorphological examination, in 24 cases (48%), patterns pathognomonic for demodectic infection were found. Such patterns, which could indicate the presence of a demodex mite, were cystic formations, as well as fragments of a dead parasite. We noted their presence not only in the tumor tissue, but also in the adjacent hair follicles, sebaceous glands, and subepidermal stroma. It is no coincidence that the authors who previously noted the presence of pigmented elements in keratopapillomas or seborrheic keratomas could not explain their origin, resorting to putting forward such fantastic assumptions as the presence of "symbiosis of melanoblasts and epithelial cells, which ensures the transfer of pigment from the first to the second".
CONCLUSIONS: Histomorphological study of serial sections allows us to see the successive stages of transformation of cavity intratissue defects created by the activity of the mite into horn cysts. Cavity defects are the main evidence of demodectic infestation. They occur in basal cell and squamous cell carcinomas, xanthomas, sebaceous adenomas, etc. tumors of the eyelids, but only in keratoacanthomas and senile keratomas they can turn into corneal cysts, which can be assumed to be due to the differentiating potential of these neoplasms.},
}
MeSH Terms:
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Humans
Animals
*Papilloma/pathology/parasitology/diagnostic imaging
*Mites
Eyelid Neoplasms/pathology/parasitology/diagnostic imaging
Male
Female
Mite Infestations/pathology/parasitology
Aged
Keratosis, Actinic/pathology
Middle Aged
RevDate: 2024-10-23
Rhizobial variation, more than plant variation, mediates plant symbiotic and fitness responses to herbicide stress.
Ecology [Epub ahead of print].
Symbiotic mutualisms provide critical ecosystem services throughout the world. Anthropogenic stressors, however, may disrupt mutualistic interactions and impact ecosystem health. The plant-rhizobia symbiosis promotes plant growth and contributes to the nitrogen (N) cycle. While off-target herbicide exposure is recognized as a significant stressor impacting wild plants, we lack knowledge about how it affects the symbiotic relationship between plants and rhizobia. Moreover, we do not know whether the impact of herbicide exposure on symbiotic traits or plant fitness might be ameliorated by plant or rhizobial genetic variation. To address these gaps, we conducted a greenhouse study where we grew 17 full-sibling genetic families of red clover (Trifolium pratense) either alone (uninoculated) or in symbiosis with one of two genetic strains of rhizobia (Rhizobium leguminosarum) and exposed them to a concentration of the herbicide dicamba that simulated "drift" (i.e., off-target atmospheric movement) or a control solution. We recorded responses in immediate vegetative injury, key features of the plant-rhizobia mutualism (nodule number, nodule size, and N fixation), mutualism outcomes, and plant fitness (biomass). In general, we found that rhizobial variation more than plant variation determined outcomes of mutualism and plant fitness in response to herbicide exposure. Herbicide damage response depended on plant family, but also whether plants were inoculated with rhizobia and if so, with which strain. Rhizobial strain variation determined nodule number and size, but this was herbicide treatment-dependent. In contrast, strain and herbicide treatment independently impacted symbiotic N fixation. And while herbicide exposure significantly reduced plant fitness, this effect depended on inoculation state. Furthermore, the differential fitness benefits that the two rhizobial strains provided plants seemed to diminish under herbicidal conditions. Altogether, these findings suggest that exposure to low levels of herbicide impact key components of the plant-rhizobia mutualism as well as plant fitness, but genetic variation in the partners determines the magnitude and/or direction of these effects. In particular, our results highlight a strong role of rhizobial strain identity in driving both symbiotic and plant growth responses to herbicide stress.
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@article {pmid39440990,
year = {2024},
author = {Iriart, V and Rarick, EM and Ashman, TL},
title = {Rhizobial variation, more than plant variation, mediates plant symbiotic and fitness responses to herbicide stress.},
journal = {Ecology},
volume = {},
number = {},
pages = {e4426},
doi = {10.1002/ecy.4426},
pmid = {39440990},
issn = {1939-9170},
support = {1747452//National Science Foundation Graduate Research Fellowship Program/ ; //Botanical Society of America/ ; //Phipps Conservatory and Botanical Gardens, Botany in Action Fellowship/ ; //Graduate Women in Science/ ; },
abstract = {Symbiotic mutualisms provide critical ecosystem services throughout the world. Anthropogenic stressors, however, may disrupt mutualistic interactions and impact ecosystem health. The plant-rhizobia symbiosis promotes plant growth and contributes to the nitrogen (N) cycle. While off-target herbicide exposure is recognized as a significant stressor impacting wild plants, we lack knowledge about how it affects the symbiotic relationship between plants and rhizobia. Moreover, we do not know whether the impact of herbicide exposure on symbiotic traits or plant fitness might be ameliorated by plant or rhizobial genetic variation. To address these gaps, we conducted a greenhouse study where we grew 17 full-sibling genetic families of red clover (Trifolium pratense) either alone (uninoculated) or in symbiosis with one of two genetic strains of rhizobia (Rhizobium leguminosarum) and exposed them to a concentration of the herbicide dicamba that simulated "drift" (i.e., off-target atmospheric movement) or a control solution. We recorded responses in immediate vegetative injury, key features of the plant-rhizobia mutualism (nodule number, nodule size, and N fixation), mutualism outcomes, and plant fitness (biomass). In general, we found that rhizobial variation more than plant variation determined outcomes of mutualism and plant fitness in response to herbicide exposure. Herbicide damage response depended on plant family, but also whether plants were inoculated with rhizobia and if so, with which strain. Rhizobial strain variation determined nodule number and size, but this was herbicide treatment-dependent. In contrast, strain and herbicide treatment independently impacted symbiotic N fixation. And while herbicide exposure significantly reduced plant fitness, this effect depended on inoculation state. Furthermore, the differential fitness benefits that the two rhizobial strains provided plants seemed to diminish under herbicidal conditions. Altogether, these findings suggest that exposure to low levels of herbicide impact key components of the plant-rhizobia mutualism as well as plant fitness, but genetic variation in the partners determines the magnitude and/or direction of these effects. In particular, our results highlight a strong role of rhizobial strain identity in driving both symbiotic and plant growth responses to herbicide stress.},
}
RevDate: 2024-10-24
Transcriptomic insights into mycorrhizal interactions with tomato root: a comparative study of short- and long-term post-inoculation responses.
Frontiers in genetics, 15:1434761.
BACKGROUND: Arbuscular mycorrhiza (AM) refers to a symbiotic association between plant roots and fungi that enhances the uptake of mineral nutrients from the soil and enables the plant to tolerate abiotic and biotic stresses. Although previously reported RNA-seq analyses have identified large numbers of AM-responsive genes in model plants, such as Solanum lycopersicum L., further studies are underway to comprehensively understand the complex interactions between plant roots and AM, especially in terms of the short- and long-term responses after inoculation.
RESULTS: Herein, we used RNA-seq technology to obtain the transcriptomes of tomato roots inoculated with the fungus Rhizophagus irregularis at 7 and 30 days post inoculation (dpi). Of the 1,019 differentially expressed genes (DEGs) in tomato roots, 635 genes showed differential expressions between mycorrhizal and non-mycorrhizal associations at the two time points. The number of upregulated DEGs far exceeded the number of downregulated ones at 7 dpi, and this difference decreased at 30 dpi. Several notable genes were particularly involved in the plant defense, plant growth and development, ion transport, and biological processes, namely, GABAT, AGP, POD, NQO1, MT4, MTA, and AROGP3. In addition, the Kyoto encyclopedia of genes and genomes pathway enrichment analysis revealed that some of the genes were involved in different pathways, including those of ascorbic acid (AFRR, GME1, and APX), metabolism (CYP, GAPC2, and CAM2), and sterols (CYC1 and HMGR), as well as genes related to cell division and cell cycle (CDKB2 and PCNA).
CONCLUSION: These findings provide valuable new data on AM-responsive genes in tomato roots at both short- and long-term postinoculation stages, enabling the deciphering of biological interactions between tomato roots and symbiotic fungi.
Additional Links: PMID-39440244
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Citation:
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@article {pmid39440244,
year = {2024},
author = {Abdelsattar, M and Soliman, MS and Mohamed, RA and Radwan, KH and El-Mahdy, MM and Mousa, KH and Khalil, SRM and Osman, E and Alameldin, HF and Hussein, A and Hassanein, SE and Abdallah, NA and Alsamman, AM and Osama, O},
title = {Transcriptomic insights into mycorrhizal interactions with tomato root: a comparative study of short- and long-term post-inoculation responses.},
journal = {Frontiers in genetics},
volume = {15},
number = {},
pages = {1434761},
pmid = {39440244},
issn = {1664-8021},
abstract = {BACKGROUND: Arbuscular mycorrhiza (AM) refers to a symbiotic association between plant roots and fungi that enhances the uptake of mineral nutrients from the soil and enables the plant to tolerate abiotic and biotic stresses. Although previously reported RNA-seq analyses have identified large numbers of AM-responsive genes in model plants, such as Solanum lycopersicum L., further studies are underway to comprehensively understand the complex interactions between plant roots and AM, especially in terms of the short- and long-term responses after inoculation.
RESULTS: Herein, we used RNA-seq technology to obtain the transcriptomes of tomato roots inoculated with the fungus Rhizophagus irregularis at 7 and 30 days post inoculation (dpi). Of the 1,019 differentially expressed genes (DEGs) in tomato roots, 635 genes showed differential expressions between mycorrhizal and non-mycorrhizal associations at the two time points. The number of upregulated DEGs far exceeded the number of downregulated ones at 7 dpi, and this difference decreased at 30 dpi. Several notable genes were particularly involved in the plant defense, plant growth and development, ion transport, and biological processes, namely, GABAT, AGP, POD, NQO1, MT4, MTA, and AROGP3. In addition, the Kyoto encyclopedia of genes and genomes pathway enrichment analysis revealed that some of the genes were involved in different pathways, including those of ascorbic acid (AFRR, GME1, and APX), metabolism (CYP, GAPC2, and CAM2), and sterols (CYC1 and HMGR), as well as genes related to cell division and cell cycle (CDKB2 and PCNA).
CONCLUSION: These findings provide valuable new data on AM-responsive genes in tomato roots at both short- and long-term postinoculation stages, enabling the deciphering of biological interactions between tomato roots and symbiotic fungi.},
}
RevDate: 2024-10-23
Pathogenicity of Bacillus subtilis Against Symbiotic Fungus of Euwallacea fornicates (Coleoptera: Scolytidae) From South India.
Journal of basic microbiology [Epub ahead of print].
In India, the shot-hole borer, Euwallacea fornicates, commonly known as the tea borer, infests the galleries of tea plant twigs under natural conditions and is a major pest of tea. The current investigation focuses on the antagonistic ability of Bacillus subtilis to directly inhibit the growth of plant pathogens in two different climatic regions of tea-growing area. The evaluation reveals that (a) B. subtilis can directly suppress the growth of plant pathogens (b) in the in vitro evaluation; the B. subtilis suppressed the growth of the Fusarium ambrossium, which is the nourishment for the ambrosia beetle, (c) it also revealed that the antagonistic microbes and the entomopathogens are able to control the pest population of the shot hole borer of tea. The impact of B. subtilis on mycelial growth, sporulation, and spore germination of F. ambrosium in agar medium was observed. In the field condition on the post-treatment assessments shows an average decline of 40% in both foliar and soil drenching. Hence, we recommend the antagonistic bacterium B. subtilis for including as an IPM for the management of shot hole borer in tea.
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@article {pmid39439270,
year = {2024},
author = {Ashif, K and Rabeesh, T},
title = {Pathogenicity of Bacillus subtilis Against Symbiotic Fungus of Euwallacea fornicates (Coleoptera: Scolytidae) From South India.},
journal = {Journal of basic microbiology},
volume = {},
number = {},
pages = {e2400455},
doi = {10.1002/jobm.202400455},
pmid = {39439270},
issn = {1521-4028},
support = {//This study was supported by the National Tea Research Foundation (grant no. 208/2019)./ ; },
abstract = {In India, the shot-hole borer, Euwallacea fornicates, commonly known as the tea borer, infests the galleries of tea plant twigs under natural conditions and is a major pest of tea. The current investigation focuses on the antagonistic ability of Bacillus subtilis to directly inhibit the growth of plant pathogens in two different climatic regions of tea-growing area. The evaluation reveals that (a) B. subtilis can directly suppress the growth of plant pathogens (b) in the in vitro evaluation; the B. subtilis suppressed the growth of the Fusarium ambrossium, which is the nourishment for the ambrosia beetle, (c) it also revealed that the antagonistic microbes and the entomopathogens are able to control the pest population of the shot hole borer of tea. The impact of B. subtilis on mycelial growth, sporulation, and spore germination of F. ambrosium in agar medium was observed. In the field condition on the post-treatment assessments shows an average decline of 40% in both foliar and soil drenching. Hence, we recommend the antagonistic bacterium B. subtilis for including as an IPM for the management of shot hole borer in tea.},
}
RevDate: 2024-10-23
Peanut-Colonized Piriformospora indica Enhanced Drought Tolerance by Modulating the Enzymes and Expression of Drought-Related Genes.
Journal of basic microbiology [Epub ahead of print].
Peanut (Arachis hypogaea L.) is an important cash and oil seed crop, mostly distributed in arid and semi-arid areas. In recent years, due to the influence of atmospheric circulation anomalies and other factors, drought has become frequent and increasingly serious in China. This has posed serious challenges to peanut production. The objective of this study was to investigate the potential of the endophytic fungus Piriformospora indica to form a symbiotic relationship with peanut plants and to evaluate the drought tolerance of P. indica-colonized peanut plants subjected to a simulated drought stress treatment using 20% polyethylene glycol 6000 (PEG6000). The endophytic fungus P. indica affected the physiological characteristics of the host plant by colonizing the plant roots, thereby conferring greater resistance to drought stress. This fungus strongly colonized the roots of peanuts and was found to enhance root activity after 24 h of P. indica colonization under PEG6000. Catalase (CAT) and peroxidase (POD) activities were increased at 24 h in peanut leaves colonized with P. indica. Expression of drought-related genes, such as AhNCED1, AhP5CS, and DREB2A was upregulated at 24 h of P. indica colonization. In addition, after PEG6000 treatment, proline, soluble protein, and abscisic acid (ABA) concentrations in plants were increased, while the accumulation of malondialdehyde (MDA), and hydrogen peroxide (H2O2) was decreased in P. indica colonized peanut. In conclusion, P. indica mediated peanut plant protection against the detrimental effects of drought resulted from enhanced antioxidant enzyme activities, and the upregulated expression of drought-related genes for lower membrane damage.
Additional Links: PMID-39439269
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@article {pmid39439269,
year = {2024},
author = {Gao, J and Wang, C and Tian, PC and Liu, C and Ahsan, T and Wei, Y and Huang, YQ and Zhang, SH},
title = {Peanut-Colonized Piriformospora indica Enhanced Drought Tolerance by Modulating the Enzymes and Expression of Drought-Related Genes.},
journal = {Journal of basic microbiology},
volume = {},
number = {},
pages = {e2400305},
doi = {10.1002/jobm.202400305},
pmid = {39439269},
issn = {1521-4028},
support = {//This research was supported by the Ministry of Science and Technology of the People's Republic of China, National Key Research and Development Program (No. 2022YFA1304401-05) and Basic Scientific Research Projects of Colleges and Universities in Liaoning Province (No. LJKMZ20221044)./ ; },
abstract = {Peanut (Arachis hypogaea L.) is an important cash and oil seed crop, mostly distributed in arid and semi-arid areas. In recent years, due to the influence of atmospheric circulation anomalies and other factors, drought has become frequent and increasingly serious in China. This has posed serious challenges to peanut production. The objective of this study was to investigate the potential of the endophytic fungus Piriformospora indica to form a symbiotic relationship with peanut plants and to evaluate the drought tolerance of P. indica-colonized peanut plants subjected to a simulated drought stress treatment using 20% polyethylene glycol 6000 (PEG6000). The endophytic fungus P. indica affected the physiological characteristics of the host plant by colonizing the plant roots, thereby conferring greater resistance to drought stress. This fungus strongly colonized the roots of peanuts and was found to enhance root activity after 24 h of P. indica colonization under PEG6000. Catalase (CAT) and peroxidase (POD) activities were increased at 24 h in peanut leaves colonized with P. indica. Expression of drought-related genes, such as AhNCED1, AhP5CS, and DREB2A was upregulated at 24 h of P. indica colonization. In addition, after PEG6000 treatment, proline, soluble protein, and abscisic acid (ABA) concentrations in plants were increased, while the accumulation of malondialdehyde (MDA), and hydrogen peroxide (H2O2) was decreased in P. indica colonized peanut. In conclusion, P. indica mediated peanut plant protection against the detrimental effects of drought resulted from enhanced antioxidant enzyme activities, and the upregulated expression of drought-related genes for lower membrane damage.},
}
RevDate: 2024-10-24
Unveiling the hidden world: How arbuscular mycorrhizal fungi and its regulated core fungi modify the composition and metabolism of soybean rhizosphere microbiome.
Environmental microbiome, 19(1):78.
BACKGROUND: The symbiosis between arbuscular mycorrhizal fungi (AMF) and plants often stimulates plant growth, increases agricultural yield, reduces costs, thereby providing significant economic benefits. AMF can also benefit plants through affecting the rhizosphere microbial community, but the underlying mechanisms remain unclear. Using Rhizophagus intraradices as a model AMF species, we assessed how AMF influences the bacterial composition and functional diversity through 16 S rRNA gene sequencing and non-targeted metabolomics analysis in the rhizosphere of aluminum-sensitive soybean that were inoculated with pathogenic fungus Nigrospora oryzae and phosphorus-solubilizing fungus Talaromyces verruculosus in an acidic soil.
RESULTS: The inoculation of R. intraradices, N. oryzae and T. verruculosus didn't have a significant influence on the levels of soil C, N, and P, or various plant characteristics such as seed weight, crude fat and protein content. However, their inoculation affected the structure, function and nutrient dynamics of the resident bacterial community. The co-inoculation of T. verruculosus and R. intraradices increased the relative abundance of Pseudomonas psychrotolerans, which was capable of N-fixing and was related to cry-for-help theory (plants signal for beneficial microbes when under stress), within the rhizosphere. R. intraradices increased the expression of metabolic pathways associated with the synthesis of unsaturated fatty acids, which was known to enhance plant resistance under adverse environmental conditions. The inoculation of N. oryzae stimulated the stress response inside the soil environment by enriching the polyene macrolide antifungal antibiotic-producing bacterial genus Streptomyces in the root endosphere and upregulating two antibacterial activity metabolic pathways associated with steroid biosynthesis pathways in the rhizosphere. Although inoculation of pathogenic fungus N. oryzae enriched Bradyrhizobium and increased soil urease activity, it had no significant effects on biomass and N content of soybean. Lastly, the host niches exhibited differences in the composition of the bacterial community, with most N-fixing bacteria accumulating in the endosphere and Rhizobium vallis only detected in the endosphere.
CONCLUSIONS: Our findings demonstrate that intricate interactions between AMF, associated core fungi, and the soybean root-associated ecological niches co-mediate the regulation of soybean growth, the dynamics of rhizosphere soil nutrients, and the composition, function, and metabolisms of the root-associated microbiome in an acidic soil.
Additional Links: PMID-39439005
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Citation:
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@article {pmid39439005,
year = {2024},
author = {Yang, M and Song, Y and Ma, H and Li, Z and Ding, J and Yin, T and Niu, K and Sun, S and Qi, J and Lu, G and Fazal, A and Yang, Y and Wen, Z},
title = {Unveiling the hidden world: How arbuscular mycorrhizal fungi and its regulated core fungi modify the composition and metabolism of soybean rhizosphere microbiome.},
journal = {Environmental microbiome},
volume = {19},
number = {1},
pages = {78},
pmid = {39439005},
issn = {2524-6372},
support = {2023M731604//China Postdoctoral Science Foundation/ ; 2023T160299//China Postdoctoral Science Foundation/ ; 42377327//National Natural Science Foundation of China/ ; 32101383//National Natural Science Foundation of China/ ; IRT_14R27//Program for Changjiang Scholars and Innovative Research Team in University/ ; },
abstract = {BACKGROUND: The symbiosis between arbuscular mycorrhizal fungi (AMF) and plants often stimulates plant growth, increases agricultural yield, reduces costs, thereby providing significant economic benefits. AMF can also benefit plants through affecting the rhizosphere microbial community, but the underlying mechanisms remain unclear. Using Rhizophagus intraradices as a model AMF species, we assessed how AMF influences the bacterial composition and functional diversity through 16 S rRNA gene sequencing and non-targeted metabolomics analysis in the rhizosphere of aluminum-sensitive soybean that were inoculated with pathogenic fungus Nigrospora oryzae and phosphorus-solubilizing fungus Talaromyces verruculosus in an acidic soil.
RESULTS: The inoculation of R. intraradices, N. oryzae and T. verruculosus didn't have a significant influence on the levels of soil C, N, and P, or various plant characteristics such as seed weight, crude fat and protein content. However, their inoculation affected the structure, function and nutrient dynamics of the resident bacterial community. The co-inoculation of T. verruculosus and R. intraradices increased the relative abundance of Pseudomonas psychrotolerans, which was capable of N-fixing and was related to cry-for-help theory (plants signal for beneficial microbes when under stress), within the rhizosphere. R. intraradices increased the expression of metabolic pathways associated with the synthesis of unsaturated fatty acids, which was known to enhance plant resistance under adverse environmental conditions. The inoculation of N. oryzae stimulated the stress response inside the soil environment by enriching the polyene macrolide antifungal antibiotic-producing bacterial genus Streptomyces in the root endosphere and upregulating two antibacterial activity metabolic pathways associated with steroid biosynthesis pathways in the rhizosphere. Although inoculation of pathogenic fungus N. oryzae enriched Bradyrhizobium and increased soil urease activity, it had no significant effects on biomass and N content of soybean. Lastly, the host niches exhibited differences in the composition of the bacterial community, with most N-fixing bacteria accumulating in the endosphere and Rhizobium vallis only detected in the endosphere.
CONCLUSIONS: Our findings demonstrate that intricate interactions between AMF, associated core fungi, and the soybean root-associated ecological niches co-mediate the regulation of soybean growth, the dynamics of rhizosphere soil nutrients, and the composition, function, and metabolisms of the root-associated microbiome in an acidic soil.},
}
RevDate: 2024-10-22
The CEP peptide-CRA2 receptor module promotes arbuscular mycorrhizal symbiosis.
Current biology : CB pii:S0960-9822(24)01326-5 [Epub ahead of print].
C-terminally encoded peptides (CEPs) are small secreted signaling peptides that promote nitrogen-fixing root nodulation symbiosis in legumes, depending on soil mineral nitrogen availability.[1] In Medicago truncatula, their action is mediated by the leucine-rich repeat receptor-like protein kinase COMPACT ROOT ARCHITECTURE 2 (CRA2).[2][,][3][,][4] Like most land plants, under inorganic phosphate limitation, M. truncatula establishes another root endosymbiotic interaction with arbuscular fungi, the arbuscular mycorrhizal symbiosis (AMS). Because this interaction is beneficial for the plant but has a high energetic cost, it is tightly controlled by host plants to limit fungal infections mainly depending on phosphate availability.[5] We show in this study that the expression of a subset of CEP-encoding genes is enhanced in the low-phosphate conditions and that overexpression of the low-phosphate-induced MtCEP1 gene, previously shown to promote the nitrogen-fixing root nodulation symbiosis, enhances AMS from the initial entry point of the fungi. Conversely, a loss-of-function mutation of the CRA2 receptor required for mediating CEP peptide action[2] decreases the endomycorrhizal interaction from the same initial fungal entry stage. Transcriptomic analyses revealed that the cra2 mutant is negatively affected in the regulation of key phosphate transport and response genes as well as in the biosynthesis of strigolactone hormones that are required for establishing AMS. Accordingly, strigolactone contents were drastically decreased in cra2 mutant roots. Overall, we showed that the CEP/CRA2 pathway promotes both root nodulation and AMS in legume plants, depending on soil mineral nutrient availability.
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@article {pmid39437785,
year = {2024},
author = {Pedinotti, L and Teyssendier de la Serve, J and Roudaire, T and San Clemente, H and Aguilar, M and Kohlen, W and Frugier, F and Frei Dit Frey, N},
title = {The CEP peptide-CRA2 receptor module promotes arbuscular mycorrhizal symbiosis.},
journal = {Current biology : CB},
volume = {},
number = {},
pages = {},
doi = {10.1016/j.cub.2024.09.058},
pmid = {39437785},
issn = {1879-0445},
abstract = {C-terminally encoded peptides (CEPs) are small secreted signaling peptides that promote nitrogen-fixing root nodulation symbiosis in legumes, depending on soil mineral nitrogen availability.[1] In Medicago truncatula, their action is mediated by the leucine-rich repeat receptor-like protein kinase COMPACT ROOT ARCHITECTURE 2 (CRA2).[2][,][3][,][4] Like most land plants, under inorganic phosphate limitation, M. truncatula establishes another root endosymbiotic interaction with arbuscular fungi, the arbuscular mycorrhizal symbiosis (AMS). Because this interaction is beneficial for the plant but has a high energetic cost, it is tightly controlled by host plants to limit fungal infections mainly depending on phosphate availability.[5] We show in this study that the expression of a subset of CEP-encoding genes is enhanced in the low-phosphate conditions and that overexpression of the low-phosphate-induced MtCEP1 gene, previously shown to promote the nitrogen-fixing root nodulation symbiosis, enhances AMS from the initial entry point of the fungi. Conversely, a loss-of-function mutation of the CRA2 receptor required for mediating CEP peptide action[2] decreases the endomycorrhizal interaction from the same initial fungal entry stage. Transcriptomic analyses revealed that the cra2 mutant is negatively affected in the regulation of key phosphate transport and response genes as well as in the biosynthesis of strigolactone hormones that are required for establishing AMS. Accordingly, strigolactone contents were drastically decreased in cra2 mutant roots. Overall, we showed that the CEP/CRA2 pathway promotes both root nodulation and AMS in legume plants, depending on soil mineral nutrient availability.},
}
RevDate: 2024-10-22
Harnessing microbial biofilms in soil ecosystems: Enhancing nutrient cycling, stress resilience, and sustainable agriculture.
Journal of environmental management, 370:122973 pii:S0301-4797(24)02959-1 [Epub ahead of print].
Soil ecosystems are complex networks of microorganisms that play pivotal roles in nutrient cycling, stress resilience, and the provision of ecosystem services. Among these microbial communities, soil biofilms, and complex aggregations of microorganisms embedded within extracellular polymeric substances (EPS) exert significant influence on soil health and function. This review delves into the dynamics of soil biofilms, highlighting their structural intricacies and the mechanisms by which they facilitate nutrient cycling, and discusses how biofilms enhance the degradation of pollutants through the action of extracellular enzymes and horizontal gene transfer, contributing to soil detoxification and fertility. Furthermore, the role of soil biofilms in stress resilience is underscored, as they form symbiotic relationships with plants, bolstering their growth and resistance to environmental stressors. The review also explores the ecological functions of biofilms in enhancing soil structure stability by promoting aggregate formation, which is crucial for water retention and aeration. By integrating these insights, we aim to provide a comprehensive understanding of the multifaceted benefits of biofilms in soil ecosystems. This knowledge is essential for developing strategies to manipulate soil biofilms to improve agricultural productivity and ecological sustainability. This review also identifies research gaps and emphasizes the need for practical applications of biofilms in sustainable agriculture.
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@article {pmid39437688,
year = {2024},
author = {Zhang, B and Hu, X and Zhao, D and Wang, Y and Qu, J and Tao, Y and Kang, Z and Yu, H and Zhang, J and Zhang, Y},
title = {Harnessing microbial biofilms in soil ecosystems: Enhancing nutrient cycling, stress resilience, and sustainable agriculture.},
journal = {Journal of environmental management},
volume = {370},
number = {},
pages = {122973},
doi = {10.1016/j.jenvman.2024.122973},
pmid = {39437688},
issn = {1095-8630},
abstract = {Soil ecosystems are complex networks of microorganisms that play pivotal roles in nutrient cycling, stress resilience, and the provision of ecosystem services. Among these microbial communities, soil biofilms, and complex aggregations of microorganisms embedded within extracellular polymeric substances (EPS) exert significant influence on soil health and function. This review delves into the dynamics of soil biofilms, highlighting their structural intricacies and the mechanisms by which they facilitate nutrient cycling, and discusses how biofilms enhance the degradation of pollutants through the action of extracellular enzymes and horizontal gene transfer, contributing to soil detoxification and fertility. Furthermore, the role of soil biofilms in stress resilience is underscored, as they form symbiotic relationships with plants, bolstering their growth and resistance to environmental stressors. The review also explores the ecological functions of biofilms in enhancing soil structure stability by promoting aggregate formation, which is crucial for water retention and aeration. By integrating these insights, we aim to provide a comprehensive understanding of the multifaceted benefits of biofilms in soil ecosystems. This knowledge is essential for developing strategies to manipulate soil biofilms to improve agricultural productivity and ecological sustainability. This review also identifies research gaps and emphasizes the need for practical applications of biofilms in sustainable agriculture.},
}
RevDate: 2024-10-22
Response of bacterial and fungal composition in tailings to Mn pollution.
Journal of hazardous materials, 480:136223 pii:S0304-3894(24)02802-4 [Epub ahead of print].
Microorganisms are crucial for natural remediation of heavy metal pollution in mining areas. The regional survey and process analysis of Mn mine microbes is still limited. We investigated microbial species composition in tailings and adjacent soils of seven typical Mn mining areas in wet mid-subtropical China. The Mn bioavailable content in tailings was 55 times higher than in soils. Compared to soils, the heavy metal pollution in tailings reduced the hydrolase activities and microbial species diversity by 97 % and 38 %, respectively. The co-occurrence network of bacterial and fungal species in tailings was dominated by symbiosis and synergism, and their network complexity was lower than that in soils. Linear discriminant analysis of effect size revealed that Ralstonia, Acidisoma, and Talaromyces were the species most stimulated by Mn pollution because their relative and absolute abundance in tailings was much higher than those in soils (p < 0.001). These key species defined the co-occurrence networks and affected metabolic pathways of microbial communities. Electrical conductivity and its interaction with Mn bioavailability strongly affected tailings microbial key species. This work identified the key species adapted to extreme Mn pollution in tailings, which can be used for bioremediation and maintenance of ecosystem functions in Mn-contaminated soils.
Additional Links: PMID-39437468
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@article {pmid39437468,
year = {2024},
author = {Lei, J and Dan, Q and Yan, W and Liu, T and Kuzyakov, Y and Wang, W and Xu, Y and Liu, J and Fang, Y and Wang, J and Wu, X},
title = {Response of bacterial and fungal composition in tailings to Mn pollution.},
journal = {Journal of hazardous materials},
volume = {480},
number = {},
pages = {136223},
doi = {10.1016/j.jhazmat.2024.136223},
pmid = {39437468},
issn = {1873-3336},
abstract = {Microorganisms are crucial for natural remediation of heavy metal pollution in mining areas. The regional survey and process analysis of Mn mine microbes is still limited. We investigated microbial species composition in tailings and adjacent soils of seven typical Mn mining areas in wet mid-subtropical China. The Mn bioavailable content in tailings was 55 times higher than in soils. Compared to soils, the heavy metal pollution in tailings reduced the hydrolase activities and microbial species diversity by 97 % and 38 %, respectively. The co-occurrence network of bacterial and fungal species in tailings was dominated by symbiosis and synergism, and their network complexity was lower than that in soils. Linear discriminant analysis of effect size revealed that Ralstonia, Acidisoma, and Talaromyces were the species most stimulated by Mn pollution because their relative and absolute abundance in tailings was much higher than those in soils (p < 0.001). These key species defined the co-occurrence networks and affected metabolic pathways of microbial communities. Electrical conductivity and its interaction with Mn bioavailability strongly affected tailings microbial key species. This work identified the key species adapted to extreme Mn pollution in tailings, which can be used for bioremediation and maintenance of ecosystem functions in Mn-contaminated soils.},
}
RevDate: 2024-10-22
Functional analysis of cyclic diguanylate-modulating proteins in Vibrio fischeri.
mSystems [Epub ahead of print].
As bacterial symbionts transition from a motile free-living state to a sessile biofilm state, they must coordinate behavior changes suitable to each lifestyle. Cyclic diguanylate (c-di-GMP) is an intracellular signaling molecule that can regulate this transition, and it is synthesized by diguanylate cyclase (DGC) enzymes and degraded by phosphodiesterase (PDE) enzymes. Generally, c-di-GMP inhibits motility and promotes biofilm formation. While c-di-GMP and the enzymes that contribute to its metabolism have been well studied in pathogens, considerably less focus has been placed on c-di-GMP regulation in beneficial symbionts. Vibrio fischeri is the sole beneficial symbiont of the Hawaiian bobtail squid (Euprymna scolopes) light organ, and the bacterium requires both motility and biofilm formation to efficiently colonize. c-di-GMP regulates swimming motility and cellulose exopolysaccharide production in V. fischeri. The genome encodes 50 DGCs and PDEs, and while a few of these proteins have been characterized, the majority have not undergone comprehensive characterization. In this study, we use protein overexpression to systematically characterize the functional potential of all 50 V. fischeri proteins. All 28 predicted DGCs and 10 of the 14 predicted PDEs displayed at least one phenotype consistent with their predicted function, and a majority of each displayed multiple phenotypes. Finally, active site mutant analysis of proteins with the potential for both DGC and PDE activities revealed potential activities for these proteins. This work presents a systems-level functional analysis of a family of signaling proteins in a tractable animal symbiont and will inform future efforts to characterize the roles of individual proteins during lifestyle transitions.IMPORTANCECyclic diguanylate (c-di-GMP) is a critical second messenger that mediates bacterial behaviors, and Vibrio fischeri colonization of its Hawaiian bobtail squid host presents a tractable model in which to interrogate the role of c-di-GMP during animal colonization. This work provides systems-level characterization of the 50 proteins predicted to modulate c-di-GMP levels. By combining multiple assays, we generated a rich understanding of which proteins have the capacity to influence c-di-GMP levels and behaviors. Our functional approach yielded insights into how proteins with domains to both synthesize and degrade c-di-GMP may impact bacterial behaviors. Finally, we integrated published data to provide a broader picture of each of the 50 proteins analyzed. This study will inform future work to define specific pathways by which c-di-GMP regulates symbiotic behaviors and transitions.
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@article {pmid39436151,
year = {2024},
author = {Isenberg, RY and Holschbach, CS and Gao, J and Mandel, MJ},
title = {Functional analysis of cyclic diguanylate-modulating proteins in Vibrio fischeri.},
journal = {mSystems},
volume = {},
number = {},
pages = {e0095624},
doi = {10.1128/msystems.00956-24},
pmid = {39436151},
issn = {2379-5077},
abstract = {As bacterial symbionts transition from a motile free-living state to a sessile biofilm state, they must coordinate behavior changes suitable to each lifestyle. Cyclic diguanylate (c-di-GMP) is an intracellular signaling molecule that can regulate this transition, and it is synthesized by diguanylate cyclase (DGC) enzymes and degraded by phosphodiesterase (PDE) enzymes. Generally, c-di-GMP inhibits motility and promotes biofilm formation. While c-di-GMP and the enzymes that contribute to its metabolism have been well studied in pathogens, considerably less focus has been placed on c-di-GMP regulation in beneficial symbionts. Vibrio fischeri is the sole beneficial symbiont of the Hawaiian bobtail squid (Euprymna scolopes) light organ, and the bacterium requires both motility and biofilm formation to efficiently colonize. c-di-GMP regulates swimming motility and cellulose exopolysaccharide production in V. fischeri. The genome encodes 50 DGCs and PDEs, and while a few of these proteins have been characterized, the majority have not undergone comprehensive characterization. In this study, we use protein overexpression to systematically characterize the functional potential of all 50 V. fischeri proteins. All 28 predicted DGCs and 10 of the 14 predicted PDEs displayed at least one phenotype consistent with their predicted function, and a majority of each displayed multiple phenotypes. Finally, active site mutant analysis of proteins with the potential for both DGC and PDE activities revealed potential activities for these proteins. This work presents a systems-level functional analysis of a family of signaling proteins in a tractable animal symbiont and will inform future efforts to characterize the roles of individual proteins during lifestyle transitions.IMPORTANCECyclic diguanylate (c-di-GMP) is a critical second messenger that mediates bacterial behaviors, and Vibrio fischeri colonization of its Hawaiian bobtail squid host presents a tractable model in which to interrogate the role of c-di-GMP during animal colonization. This work provides systems-level characterization of the 50 proteins predicted to modulate c-di-GMP levels. By combining multiple assays, we generated a rich understanding of which proteins have the capacity to influence c-di-GMP levels and behaviors. Our functional approach yielded insights into how proteins with domains to both synthesize and degrade c-di-GMP may impact bacterial behaviors. Finally, we integrated published data to provide a broader picture of each of the 50 proteins analyzed. This study will inform future work to define specific pathways by which c-di-GMP regulates symbiotic behaviors and transitions.},
}
RevDate: 2024-10-22
The poplar SWEET1c glucose transporter plays a key role in the ectomycorrhizal symbiosis.
The New phytologist [Epub ahead of print].
The mutualistic interaction between ectomycorrhizal fungi and trees is characterized by the coordinated exchange of soil nutrients with soluble sugars. Despite the importance of this process, the precise mechanism by which sugars are transported from host roots to colonizing hyphae remains unclear. This study aimed to identify the specific membrane transporters responsible for the unloading of sugars at the symbiotic interface, with a focus on the role of the root Sugars Will Eventually Be Exported Transporter (SWEET) uniporters. Our study used RNA sequencing and quantitative PCR to identify PtaSWEET gene expression in Populus tremula × alba-Laccaria bicolor ectomycorrhizal root tips. Our results suggest that symbiosis-induced PtaSWEET1c is primarily responsible for transporting glucose and sucrose, as demonstrated by the yeast assays. Moreover, we used a promoter-YFP reporter to confirm the localization of the PtaSWEET1c expression in cortical cells of ectomycorrhizal rootlets, supporting its major role in supplying glucose at the symbiotic interface. Furthermore, our observations confirmed the localization of PtaSWEET1c-GFP in the plasma membrane. The inactivation of PtaSWEET1c reduced ectomycorrhizal root formation and [13]C translocation to ectomycorrhizal roots. Our findings highlight the crucial role of PtaSWEET1c in facilitating glucose and sucrose transport at the symbiotic interface of Populus-L. bicolor symbiosis.
Additional Links: PMID-39434237
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@article {pmid39434237,
year = {2024},
author = {Li, R and Shi, W and Zhang, P and Ma, J and Zou, R and Zhang, X and Kohler, A and Martin, FM and Zhang, F},
title = {The poplar SWEET1c glucose transporter plays a key role in the ectomycorrhizal symbiosis.},
journal = {The New phytologist},
volume = {},
number = {},
pages = {},
doi = {10.1111/nph.20183},
pmid = {39434237},
issn = {1469-8137},
support = {2022YFD2201200//National Key Research and Development Program of China/ ; 31901279//National Natural Science Foundation of China/ ; 32271829//National Natural Science Foundation of China/ ; ANR-11-LABX-0002-01//Laboratory of Excellence ARBRE/ ; },
abstract = {The mutualistic interaction between ectomycorrhizal fungi and trees is characterized by the coordinated exchange of soil nutrients with soluble sugars. Despite the importance of this process, the precise mechanism by which sugars are transported from host roots to colonizing hyphae remains unclear. This study aimed to identify the specific membrane transporters responsible for the unloading of sugars at the symbiotic interface, with a focus on the role of the root Sugars Will Eventually Be Exported Transporter (SWEET) uniporters. Our study used RNA sequencing and quantitative PCR to identify PtaSWEET gene expression in Populus tremula × alba-Laccaria bicolor ectomycorrhizal root tips. Our results suggest that symbiosis-induced PtaSWEET1c is primarily responsible for transporting glucose and sucrose, as demonstrated by the yeast assays. Moreover, we used a promoter-YFP reporter to confirm the localization of the PtaSWEET1c expression in cortical cells of ectomycorrhizal rootlets, supporting its major role in supplying glucose at the symbiotic interface. Furthermore, our observations confirmed the localization of PtaSWEET1c-GFP in the plasma membrane. The inactivation of PtaSWEET1c reduced ectomycorrhizal root formation and [13]C translocation to ectomycorrhizal roots. Our findings highlight the crucial role of PtaSWEET1c in facilitating glucose and sucrose transport at the symbiotic interface of Populus-L. bicolor symbiosis.},
}
RevDate: 2024-10-21
Harnessing symbiotic bacteria for disease control.
Nature reviews. Microbiology [Epub ahead of print].
Additional Links: PMID-39433905
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@article {pmid39433905,
year = {2024},
author = {Morales-Cruz, A and Baumgart, LA},
title = {Harnessing symbiotic bacteria for disease control.},
journal = {Nature reviews. Microbiology},
volume = {},
number = {},
pages = {},
pmid = {39433905},
issn = {1740-1534},
}
RevDate: 2024-10-23
CmpDate: 2024-10-21
Fungal community characteristics of the last remaining habitat of three paphiopedilum species in China.
Scientific reports, 14(1):24737.
Paphiopedilum armeniacum, Paphiopedilum wenshanense and Paphiopedilum emersonii are critically endangered wild orchids. Their populations are under severe threat, with a dramatic decline in the number of their natural distribution sites. Ex situ conservation and artificial breeding are the keys to maintaining the population to ensure the success of ex situ conservation and field return in the future. The habitat characteristics and soil nutrient information of the last remaining wild distribution sites of the three species were studied. ITS high-throughput sequencing was used to reveal the composition and structure of the soil fungal community, analyze its diversity and functional characteristics, and reveal its relationship with soil nutrients. The three species preferred to grow on low-lying, ventilated and shaded declivities with good water drainage. There were significant differences in soil alkali-hydrolyzed nitrogen and available phosphorus among the three species. There were 336 fungal species detected in the samples. On average, there were different dominant groups in the soil fungal communities of the three species. The functional groups of soil fungi within their habitats were dominated by saprophytic fungi and ectomycorrhizae, with significant differences in diversity and structure. The co-occurrence network of habitat soil fungi was mainly positive. Soil pH significantly affected soil fungal diversity within their habitats of the three paphiopedilum species. The study confirmed that the dominant groups of soil fungi were significantly correlated with soil nutrients. The three species exhibit comparable habitat inclinations, yet they display substantial variations in the composition, structure, and diversity of soil fungi. The fungal functional group is characterized by a rich presence of saprophytic fungi, a proliferation of ectomycorrhizae, and a modest occurrence of orchid mycorrhizae. The symbiotic interactions among the soil fungi associated with these three species are well-coordinated, enhancing their resilience against challenging environmental conditions. There is a significant correlation between soil environmental factors and the composition of soil fungal communities, with pH emerging as a pivotal factor regulating fungal diversity. Our research into the habitat traits and soil fungal ecosystems of the three wild Paphiopedilum species has established a cornerstone for prospective ex situ conservation measures and the eventual reestablishment of these species in their native landscapes.
Additional Links: PMID-39433552
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Citation:
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@article {pmid39433552,
year = {2024},
author = {Tian, L and An, M and Liu, F and Zhang, Y},
title = {Fungal community characteristics of the last remaining habitat of three paphiopedilum species in China.},
journal = {Scientific reports},
volume = {14},
number = {1},
pages = {24737},
pmid = {39433552},
issn = {2045-2322},
mesh = {China ; *Soil Microbiology ; *Orchidaceae/microbiology ; *Ecosystem ; *Soil/chemistry ; *Mycobiome ; *Fungi/classification/genetics/isolation & purification ; Biodiversity ; Mycorrhizae/genetics/classification ; Nitrogen/analysis ; },
abstract = {Paphiopedilum armeniacum, Paphiopedilum wenshanense and Paphiopedilum emersonii are critically endangered wild orchids. Their populations are under severe threat, with a dramatic decline in the number of their natural distribution sites. Ex situ conservation and artificial breeding are the keys to maintaining the population to ensure the success of ex situ conservation and field return in the future. The habitat characteristics and soil nutrient information of the last remaining wild distribution sites of the three species were studied. ITS high-throughput sequencing was used to reveal the composition and structure of the soil fungal community, analyze its diversity and functional characteristics, and reveal its relationship with soil nutrients. The three species preferred to grow on low-lying, ventilated and shaded declivities with good water drainage. There were significant differences in soil alkali-hydrolyzed nitrogen and available phosphorus among the three species. There were 336 fungal species detected in the samples. On average, there were different dominant groups in the soil fungal communities of the three species. The functional groups of soil fungi within their habitats were dominated by saprophytic fungi and ectomycorrhizae, with significant differences in diversity and structure. The co-occurrence network of habitat soil fungi was mainly positive. Soil pH significantly affected soil fungal diversity within their habitats of the three paphiopedilum species. The study confirmed that the dominant groups of soil fungi were significantly correlated with soil nutrients. The three species exhibit comparable habitat inclinations, yet they display substantial variations in the composition, structure, and diversity of soil fungi. The fungal functional group is characterized by a rich presence of saprophytic fungi, a proliferation of ectomycorrhizae, and a modest occurrence of orchid mycorrhizae. The symbiotic interactions among the soil fungi associated with these three species are well-coordinated, enhancing their resilience against challenging environmental conditions. There is a significant correlation between soil environmental factors and the composition of soil fungal communities, with pH emerging as a pivotal factor regulating fungal diversity. Our research into the habitat traits and soil fungal ecosystems of the three wild Paphiopedilum species has established a cornerstone for prospective ex situ conservation measures and the eventual reestablishment of these species in their native landscapes.},
}
MeSH Terms:
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China
*Soil Microbiology
*Orchidaceae/microbiology
*Ecosystem
*Soil/chemistry
*Mycobiome
*Fungi/classification/genetics/isolation & purification
Biodiversity
Mycorrhizae/genetics/classification
Nitrogen/analysis
RevDate: 2024-10-23
CmpDate: 2024-10-21
Sorlinia euscelidii gen. nov., sp. nov., a novel acetic acid bacterium isolated from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae).
International journal of systematic and evolutionary microbiology, 74(10):.
Acetic acid bacteria - belonging to the Acetobacteraceae family - are found in the gut of many sugar-feeding insects. In this study, six strains have been isolated from the hemipteran leafhopper Euscelidius variegatus. While they exhibit high 16S rRNA gene sequence similarities to uncultured members of the Acetobacteraceae family, they could not be unequivocally assigned to any particular type species. Considering the clonality of the six isolates, the EV16P[T] strain was used as a representative of this group of isolates. The genome sequence of EV16P[T] is composed of a 2.388 Mbp chromosome, with a DNA G+C content of 57 mol%. Phylogenetic analyses based on the 16S rRNA gene sequence and whole-genome multilocus sequence analysis indicate that EV16P[T] forms a monophyletic clade with the uncultivated endosymbiont of Diaphorina citri, the Candidatus Kirkpatrickella diaphorinae. Such a phylogenetic clade is positioned between those of Asaia-Swaminathania and Kozakia. The genomic distance metrics based on gene and protein sequences support the proposal that EV16P[T] is a new species belonging to a yet-undescribed genus. It is a rod-shaped Gram-stain-negative bacterium, strictly aerobic, non-motile, non-spore-forming, showing optimal growth without salt (NaCl) at 30 °C and pH of 6-7. The major quinone is Q10, and the dominant cellular fatty acids (>10%) are C18:l ω7c, C19 : 0 cyclo ω6c, C16 : 0 and C19 : 1 2OH. The polar lipid profile comprises diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, along with unidentified aminophospholipids, glycophospholipids, aminolipids and lipids. Based on a polyphasic approach, including phylogenetic, phylogenomic, genome relatedness, phenotypic and chemotaxonomic characterisations, EV16P[T] (= KCTC 8296[T], = DSM 117028[T]) is proposed as a representative of a novel species in a novel genus with the proposed name Sorlinia euscelidii gen. nov., sp. nov., in honour of Prof. Claudia Sorlini, an Italian environmental microbiologist at the University of Milan who inspired the research on microbial diversity, including symbiosis in plants and animals.
Additional Links: PMID-39432413
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@article {pmid39432413,
year = {2024},
author = {Marasco, R and Michoud, G and Seferji, KA and Gonella, E and Garuglieri, E and Rolli, E and Alma, A and Mapelli, F and Borin, S and Daffonchio, D and Crotti, E},
title = {Sorlinia euscelidii gen. nov., sp. nov., a novel acetic acid bacterium isolated from the leafhopper Euscelidius variegatus (Hemiptera: Cicadellidae).},
journal = {International journal of systematic and evolutionary microbiology},
volume = {74},
number = {10},
pages = {},
pmid = {39432413},
issn = {1466-5034},
mesh = {Animals ; *Hemiptera/microbiology ; *Phylogeny ; *RNA, Ribosomal, 16S/genetics ; *Fatty Acids/analysis/chemistry ; *DNA, Bacterial/genetics ; *Acetobacteraceae/classification/genetics/isolation & purification ; *Base Composition ; *Bacterial Typing Techniques ; *Sequence Analysis, DNA ; *Multilocus Sequence Typing ; Genome, Bacterial ; Acetic Acid/metabolism ; },
abstract = {Acetic acid bacteria - belonging to the Acetobacteraceae family - are found in the gut of many sugar-feeding insects. In this study, six strains have been isolated from the hemipteran leafhopper Euscelidius variegatus. While they exhibit high 16S rRNA gene sequence similarities to uncultured members of the Acetobacteraceae family, they could not be unequivocally assigned to any particular type species. Considering the clonality of the six isolates, the EV16P[T] strain was used as a representative of this group of isolates. The genome sequence of EV16P[T] is composed of a 2.388 Mbp chromosome, with a DNA G+C content of 57 mol%. Phylogenetic analyses based on the 16S rRNA gene sequence and whole-genome multilocus sequence analysis indicate that EV16P[T] forms a monophyletic clade with the uncultivated endosymbiont of Diaphorina citri, the Candidatus Kirkpatrickella diaphorinae. Such a phylogenetic clade is positioned between those of Asaia-Swaminathania and Kozakia. The genomic distance metrics based on gene and protein sequences support the proposal that EV16P[T] is a new species belonging to a yet-undescribed genus. It is a rod-shaped Gram-stain-negative bacterium, strictly aerobic, non-motile, non-spore-forming, showing optimal growth without salt (NaCl) at 30 °C and pH of 6-7. The major quinone is Q10, and the dominant cellular fatty acids (>10%) are C18:l ω7c, C19 : 0 cyclo ω6c, C16 : 0 and C19 : 1 2OH. The polar lipid profile comprises diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylcholine, along with unidentified aminophospholipids, glycophospholipids, aminolipids and lipids. Based on a polyphasic approach, including phylogenetic, phylogenomic, genome relatedness, phenotypic and chemotaxonomic characterisations, EV16P[T] (= KCTC 8296[T], = DSM 117028[T]) is proposed as a representative of a novel species in a novel genus with the proposed name Sorlinia euscelidii gen. nov., sp. nov., in honour of Prof. Claudia Sorlini, an Italian environmental microbiologist at the University of Milan who inspired the research on microbial diversity, including symbiosis in plants and animals.},
}
MeSH Terms:
show MeSH Terms
hide MeSH Terms
Animals
*Hemiptera/microbiology
*Phylogeny
*RNA, Ribosomal, 16S/genetics
*Fatty Acids/analysis/chemistry
*DNA, Bacterial/genetics
*Acetobacteraceae/classification/genetics/isolation & purification
*Base Composition
*Bacterial Typing Techniques
*Sequence Analysis, DNA
*Multilocus Sequence Typing
Genome, Bacterial
Acetic Acid/metabolism
RevDate: 2024-10-21
Chromosome structural rearrangements in invasive haplodiploid ambrosia beetles revealed by the genomes of Euwallacea fornicatus (Eichhoff) and Euwallacea similis (Ferrari) (Coleoptera, Curculionidae, Scolytinae).
Genome biology and evolution pii:7828916 [Epub ahead of print].
Bark and ambrosia beetles are among the most ecologically and economically damaging introduced plant pests worldwide. Life history traits including polyphagy, haplodiploidy, inbreeding polygyny and symbiosis with fungi contribute to their dispersal and impact. Species vary in their interactions with host trees, with many attacking stressed or recently dead trees, such as the globally distributed E. similis (Ferrari). Other species, like the Polyphagous Shot Hole Borer (PSHB) Euwallacea fornicatus (Eichhoff), can attack over 680 host plants and is causing considerable economic damage in several countries. Despite their notoriety, publicly accessible genomic resources for Euwallacea Hopkins species are scarce, hampering our understanding of their invasive capabilities as well as modern control measures, surveillance and management. Using a combination of long and short read sequencing platforms we assembled and annotated high quality (BUSCO > 98% complete) pseudo-chromosome level genomes for these species. Comparative macro-synteny analysis identified an increased number of pseudo-chromosome scaffolds in the haplodiploid inbreeding species of Euwallacea compared to diploid outbred species, due to fission events. This suggests that life history traits can impact chromosome structure. Further, the genome of E. fornicatus had a higher relative proportion of repetitive elements, up to 17% more, than E. similis. Metagenomic assembly pipelines identified microbiota associated with both species including Fusarium fungal symbionts and a novel Wolbachia strain. These novel genomes of haplodiploid inbreeding species will contribute to the understanding of how life history traits are related to their evolution and to the management of these invasive pests.
Additional Links: PMID-39431789
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PubMed:
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@article {pmid39431789,
year = {2024},
author = {Bickerstaff, JRM and Walsh, T and Court, L and Pandey, G and Ireland, K and Cousins, D and Caron, V and Wallenius, T and Slipinski, A and Rane, R and Escalona, HE},
title = {Chromosome structural rearrangements in invasive haplodiploid ambrosia beetles revealed by the genomes of Euwallacea fornicatus (Eichhoff) and Euwallacea similis (Ferrari) (Coleoptera, Curculionidae, Scolytinae).},
journal = {Genome biology and evolution},
volume = {},
number = {},
pages = {},
doi = {10.1093/gbe/evae226},
pmid = {39431789},
issn = {1759-6653},
abstract = {Bark and ambrosia beetles are among the most ecologically and economically damaging introduced plant pests worldwide. Life history traits including polyphagy, haplodiploidy, inbreeding polygyny and symbiosis with fungi contribute to their dispersal and impact. Species vary in their interactions with host trees, with many attacking stressed or recently dead trees, such as the globally distributed E. similis (Ferrari). Other species, like the Polyphagous Shot Hole Borer (PSHB) Euwallacea fornicatus (Eichhoff), can attack over 680 host plants and is causing considerable economic damage in several countries. Despite their notoriety, publicly accessible genomic resources for Euwallacea Hopkins species are scarce, hampering our understanding of their invasive capabilities as well as modern control measures, surveillance and management. Using a combination of long and short read sequencing platforms we assembled and annotated high quality (BUSCO > 98% complete) pseudo-chromosome level genomes for these species. Comparative macro-synteny analysis identified an increased number of pseudo-chromosome scaffolds in the haplodiploid inbreeding species of Euwallacea compared to diploid outbred species, due to fission events. This suggests that life history traits can impact chromosome structure. Further, the genome of E. fornicatus had a higher relative proportion of repetitive elements, up to 17% more, than E. similis. Metagenomic assembly pipelines identified microbiota associated with both species including Fusarium fungal symbionts and a novel Wolbachia strain. These novel genomes of haplodiploid inbreeding species will contribute to the understanding of how life history traits are related to their evolution and to the management of these invasive pests.},
}
RevDate: 2024-10-21
Simultaneous Quantitation of Persulfides, Biothiols, and Hydrogen Sulfide through Sulfur Exchange Reaction with Trityl Spin Probes.
Journal of the American Chemical Society [Epub ahead of print].
Reactive sulfur species (RSS) including persulfides (RSSHs), biothiols, and hydrogen sulfide (H2S) are key regulators in various physiological processes. To better understand the symbiotic relationship and interconversion of these RSS, it is highly desirable but challenging to develop analytical techniques that are capable of detecting and quantifying them. Herein, we report the rational design and synthesis of novel trityl-radical-based electron paramagnetic resonance (EPR) probes dubbed CT02-TNB and OX-TNB. CT02-TNB underwent fast sulfur exchange reactions with two reactive RSSHs (PS1 and PS2) which were released from their corresponding donors PSD1 and PSD2 to afford the specific conjugates. The resulting conjugates exhibit characteristic EPR spectra, thus enabling discriminative detection and quantitation of the two RSSHs. Moreover, CT02-TNB showed good response toward other RSS including glutathione (GSH), cysteine (Cys), H2S, and sulfite as well. Importantly, based on the updated EPR spectral simulation program, simultaneous quantitation of multiple RSS (e.g., PS1/GSH/Cys or PS1/GSH/H2S) by CT02-TNB was also achieved. Finally, the levels of released PS1 from PSD1 and endogenous GSH in isolated mouse livers were measured by the hydrophilic OX-TNB. This work represents the first study achieving discriminative and quantitative detection of different persulfides and other RSS by a spectroscopic method.
Additional Links: PMID-39431326
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PubMed:
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@article {pmid39431326,
year = {2024},
author = {Tan, X and Zhou, J and Yang, L and Chang, Q and Li, SY and Rockenbauer, A and Song, Y and Liu, Y},
title = {Simultaneous Quantitation of Persulfides, Biothiols, and Hydrogen Sulfide through Sulfur Exchange Reaction with Trityl Spin Probes.},
journal = {Journal of the American Chemical Society},
volume = {},
number = {},
pages = {},
doi = {10.1021/jacs.4c10266},
pmid = {39431326},
issn = {1520-5126},
abstract = {Reactive sulfur species (RSS) including persulfides (RSSHs), biothiols, and hydrogen sulfide (H2S) are key regulators in various physiological processes. To better understand the symbiotic relationship and interconversion of these RSS, it is highly desirable but challenging to develop analytical techniques that are capable of detecting and quantifying them. Herein, we report the rational design and synthesis of novel trityl-radical-based electron paramagnetic resonance (EPR) probes dubbed CT02-TNB and OX-TNB. CT02-TNB underwent fast sulfur exchange reactions with two reactive RSSHs (PS1 and PS2) which were released from their corresponding donors PSD1 and PSD2 to afford the specific conjugates. The resulting conjugates exhibit characteristic EPR spectra, thus enabling discriminative detection and quantitation of the two RSSHs. Moreover, CT02-TNB showed good response toward other RSS including glutathione (GSH), cysteine (Cys), H2S, and sulfite as well. Importantly, based on the updated EPR spectral simulation program, simultaneous quantitation of multiple RSS (e.g., PS1/GSH/Cys or PS1/GSH/H2S) by CT02-TNB was also achieved. Finally, the levels of released PS1 from PSD1 and endogenous GSH in isolated mouse livers were measured by the hydrophilic OX-TNB. This work represents the first study achieving discriminative and quantitative detection of different persulfides and other RSS by a spectroscopic method.},
}
RevDate: 2024-10-23
A small secreted protein serves as a plant-derived effector mediating symbiosis between Populus and Laccaria bicolor.
Horticulture research, 11(10):uhae232.
Additional Links: PMID-39431113
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@article {pmid39431113,
year = {2024},
author = {Liu, Y and Zhang, F and Devireddy, AR and Ployet, RA and Rush, TA and Lu, H and Hassan, MM and Yuan, G and Rajput, R and Islam, MT and Agrawal, R and Abraham, PE and Chen, JG and Muchero, W and Martin, F and Veneault-Fourrey, C and Yang, X},
title = {A small secreted protein serves as a plant-derived effector mediating symbiosis between Populus and Laccaria bicolor.},
journal = {Horticulture research},
volume = {11},
number = {10},
pages = {uhae232},
pmid = {39431113},
issn = {2662-6810},
}
RevDate: 2024-10-23
CmpDate: 2024-10-20
Phenology, voltinism, and brood development of Xylosandrus (Coleoptera: Curculionidae) ambrosia beetles in New York.
Journal of insect science (Online), 24(5):.
The ambrosia beetles Xylosandrus germanus (Blanford) and Xylosandrus crassiusculus (Motschulsky) are nonnative pests in orchards and nurseries in North America. They construct galleries in the sapwood of stressed woody hosts and culture a symbiotic fungus as food for their offspring. Preventing attacks is preferred but a better understanding of their biology may elucidate additional avenues for control. Recent phenological studies are lacking for X. germanus that is common in New York, and biological information on brood and gallery development is lacking for the less abundant X. crassiusculus. We conducted both outdoor rearing and laboratory studies to better understand the timing of key events in the maturation of their galleries, particularly associated with the symbiotic fungi. Two and a partial third summer generation were consistently observed over 2 yr for X. germanus; and thus, 3 flights of adult females (foundresses) occurred each summer from mid-April to late September. In both the field and laboratory, initial growth of the symbiotic fungus occurs within a few days of gallery initiation. The rapid development of the reproductive tract and oviposition by X. germanus appears to be stimulated by the presence of the fungus. Fungal, reproductive, and brood development are similar for the related X. crassiusculus in laboratory studies; the 2 species mainly appear to differ in size. The lag between beetle colonization and reproduction currently seems too brief to be exploited for management. Disruption of other stages in gallery development should also be explored to minimize the beetle damage if attacks cannot be prevented.
Additional Links: PMID-39427233
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@article {pmid39427233,
year = {2024},
author = {Milbrath, LR and Biazzo, J},
title = {Phenology, voltinism, and brood development of Xylosandrus (Coleoptera: Curculionidae) ambrosia beetles in New York.},
journal = {Journal of insect science (Online)},
volume = {24},
number = {5},
pages = {},
pmid = {39427233},
issn = {1536-2442},
support = {#8062-22410-007-000D//USDA-Agricultural Research Service/ ; },
mesh = {Animals ; *Weevils/microbiology/growth & development/physiology ; New York ; Female ; *Symbiosis ; Oviposition ; Seasons ; Male ; },
abstract = {The ambrosia beetles Xylosandrus germanus (Blanford) and Xylosandrus crassiusculus (Motschulsky) are nonnative pests in orchards and nurseries in North America. They construct galleries in the sapwood of stressed woody hosts and culture a symbiotic fungus as food for their offspring. Preventing attacks is preferred but a better understanding of their biology may elucidate additional avenues for control. Recent phenological studies are lacking for X. germanus that is common in New York, and biological information on brood and gallery development is lacking for the less abundant X. crassiusculus. We conducted both outdoor rearing and laboratory studies to better understand the timing of key events in the maturation of their galleries, particularly associated with the symbiotic fungi. Two and a partial third summer generation were consistently observed over 2 yr for X. germanus; and thus, 3 flights of adult females (foundresses) occurred each summer from mid-April to late September. In both the field and laboratory, initial growth of the symbiotic fungus occurs within a few days of gallery initiation. The rapid development of the reproductive tract and oviposition by X. germanus appears to be stimulated by the presence of the fungus. Fungal, reproductive, and brood development are similar for the related X. crassiusculus in laboratory studies; the 2 species mainly appear to differ in size. The lag between beetle colonization and reproduction currently seems too brief to be exploited for management. Disruption of other stages in gallery development should also be explored to minimize the beetle damage if attacks cannot be prevented.},
}
MeSH Terms:
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Animals
*Weevils/microbiology/growth & development/physiology
New York
Female
*Symbiosis
Oviposition
Seasons
Male
RevDate: 2024-10-19
Comparison of the effect of anticoccidial drug, probiotic, synbiotic, phytochemicals and vaccine in prevention and control of coccidiosis in broiler chickens challenged with Eimeria spp.
Poultry science, 103(12):104357 pii:S0032-5791(24)00935-0 [Epub ahead of print].
The objective of this study was to investigate the effects of an anti-coccidiosis drug, vaccine, probiotic, symbiotic, and phytochemicals in the prevention and control of coccidia infection in broilers. A total of 525 one-day-old Ross 308 chicks were randomly allocated to 7 experimental diets with 5 replicates of 15 birds each in a completely randomized design. Experimental diets consisted of negative control (NC) without any additives and not challenged. The other 6 groups were challenged with mixed Eimeria and fed the basal diet with no additives (Positive Control, PC) or supplemented with Coxidine 100 (1 g / 1 kg), probiotic, synbiotic, Livacox T vaccine and phytobiotic additives based on the manufacturer's recommended dose. Body weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were recorded weekly. Oocysts per gram of excreta (OPG) were determined on d 25 to 33 and 42. One bird per cage was euthanized to analyze lesion score and jejunum and ileum inflammatory genes expression. Coccidial challenge reduced WG (P < 0.05) during 15 to 28 d and vaccine treatment was more effective in improving WG and FCR on d 29 to 42 and 1 to 42 (P < 0.05) than other treatments. Birds in the PC group had higher (P < 0.05) OPG than NC group for all days and the vaccine treatment resulted in the lowest rate of OPG compared to other treatments (P < 0.05) at 27, 28, 29, 30, 32, and 33 d of age and overall average. Relative mRNA levels of IFN-γ, IL-1β and IL-10 were significantly upregulated among treatments under coccidiosis challenge in jejunum and ileum except for IL-1β expression in the ileum. In conclusion, based on the results of this study the individual characteristics of feed additives for the prevention of coccidiosis can be different depending on the type and source of feed additives, duration, and amount used, levels of oocyst inoculation and Eimeria types.
Additional Links: PMID-39426225
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@article {pmid39426225,
year = {2024},
author = {Javanmiri, E and Rahimi, S and Karimi Torshizi, MA and Nabiyan, S and Behnamifar, A and Grimes, J},
title = {Comparison of the effect of anticoccidial drug, probiotic, synbiotic, phytochemicals and vaccine in prevention and control of coccidiosis in broiler chickens challenged with Eimeria spp.},
journal = {Poultry science},
volume = {103},
number = {12},
pages = {104357},
doi = {10.1016/j.psj.2024.104357},
pmid = {39426225},
issn = {1525-3171},
abstract = {The objective of this study was to investigate the effects of an anti-coccidiosis drug, vaccine, probiotic, symbiotic, and phytochemicals in the prevention and control of coccidia infection in broilers. A total of 525 one-day-old Ross 308 chicks were randomly allocated to 7 experimental diets with 5 replicates of 15 birds each in a completely randomized design. Experimental diets consisted of negative control (NC) without any additives and not challenged. The other 6 groups were challenged with mixed Eimeria and fed the basal diet with no additives (Positive Control, PC) or supplemented with Coxidine 100 (1 g / 1 kg), probiotic, synbiotic, Livacox T vaccine and phytobiotic additives based on the manufacturer's recommended dose. Body weight gain (WG), feed intake (FI) and feed conversion ratio (FCR) were recorded weekly. Oocysts per gram of excreta (OPG) were determined on d 25 to 33 and 42. One bird per cage was euthanized to analyze lesion score and jejunum and ileum inflammatory genes expression. Coccidial challenge reduced WG (P < 0.05) during 15 to 28 d and vaccine treatment was more effective in improving WG and FCR on d 29 to 42 and 1 to 42 (P < 0.05) than other treatments. Birds in the PC group had higher (P < 0.05) OPG than NC group for all days and the vaccine treatment resulted in the lowest rate of OPG compared to other treatments (P < 0.05) at 27, 28, 29, 30, 32, and 33 d of age and overall average. Relative mRNA levels of IFN-γ, IL-1β and IL-10 were significantly upregulated among treatments under coccidiosis challenge in jejunum and ileum except for IL-1β expression in the ileum. In conclusion, based on the results of this study the individual characteristics of feed additives for the prevention of coccidiosis can be different depending on the type and source of feed additives, duration, and amount used, levels of oocyst inoculation and Eimeria types.},
}
RevDate: 2024-10-19
Symbiotic sheep milk cheese containing Moringa oleifera extract and Bifidobacterium pseudolongum INIA P2.
International journal of food microbiology, 427:110942 pii:S0168-1605(24)00386-6 [Epub ahead of print].
Healthy non-bovine functional dairy products are reaching high interest among consumers. In the present study, an aqueous polyphenol-rich Moringa oleifera extract (MoE) and a Bifidobacterium strain of human origin (B. pseudolongum INIA P2) were added, alone or in combination, for the manufacture of three experimental and one control sheep milk cheeses. In general, addition of 2.05 g of lyophilized MoE per 100 g of curd did not affect cheese dry matter or lactococci starter counts during ripening. B. pseudolongum INIA P2 showed good viability in cheese during ripening, and after simulated major gastrointestinal conditions, reaching levels above 7 log CFU / g of cheese. Cheeses with MoE showed lower pH, higher proteolysis and aminopeptidase activity than control cheese. MoE impoved functional properties, significantly (P < 0.01) increasing total phenolic content (TPC) and, especially, antioxidant capacity, with respect to control cheese. MoE modified cheese colour and volatile profile. Cheeses with MoE were darker in colour with higher red and yellow components than control cheese. Several volatile compounds were only detected in cheeses with MoE, indicating their plant origin. On top of that, increased levels of compounds originating from amino acid catabolism were present in these cheeses, as a result of their higher proteolytic and peptidolytic indexes. The symbiotic cheese with MoE and B. pseudolongum INIA P2 could confer beneficial effects on consumers' health by increasing polyphenol bioavailability and contributing to the host antioxidant capacity.
Additional Links: PMID-39426083
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@article {pmid39426083,
year = {2024},
author = {Rodríguez-Mínguez, E and Calzada, J and Sánchez, C and Vázquez, M and Ávila, M and Garde, S and Picon, A},
title = {Symbiotic sheep milk cheese containing Moringa oleifera extract and Bifidobacterium pseudolongum INIA P2.},
journal = {International journal of food microbiology},
volume = {427},
number = {},
pages = {110942},
doi = {10.1016/j.ijfoodmicro.2024.110942},
pmid = {39426083},
issn = {1879-3460},
abstract = {Healthy non-bovine functional dairy products are reaching high interest among consumers. In the present study, an aqueous polyphenol-rich Moringa oleifera extract (MoE) and a Bifidobacterium strain of human origin (B. pseudolongum INIA P2) were added, alone or in combination, for the manufacture of three experimental and one control sheep milk cheeses. In general, addition of 2.05 g of lyophilized MoE per 100 g of curd did not affect cheese dry matter or lactococci starter counts during ripening. B. pseudolongum INIA P2 showed good viability in cheese during ripening, and after simulated major gastrointestinal conditions, reaching levels above 7 log CFU / g of cheese. Cheeses with MoE showed lower pH, higher proteolysis and aminopeptidase activity than control cheese. MoE impoved functional properties, significantly (P < 0.01) increasing total phenolic content (TPC) and, especially, antioxidant capacity, with respect to control cheese. MoE modified cheese colour and volatile profile. Cheeses with MoE were darker in colour with higher red and yellow components than control cheese. Several volatile compounds were only detected in cheeses with MoE, indicating their plant origin. On top of that, increased levels of compounds originating from amino acid catabolism were present in these cheeses, as a result of their higher proteolytic and peptidolytic indexes. The symbiotic cheese with MoE and B. pseudolongum INIA P2 could confer beneficial effects on consumers' health by increasing polyphenol bioavailability and contributing to the host antioxidant capacity.},
}
RevDate: 2024-10-22
CmpDate: 2024-10-18
NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars.
Scientific reports, 14(1):24541.
The symbiotic relationship between legumes and rhizobia is known to be influenced by specific rhizobial type III effectors (T3Es) in certain cases. In this study, we present evidence that the symbiosis between Vigna radiata and Bradyrhizobium elkanii USDA61 is controlled by a T3E called NopP2, and this interaction is highly dependent on the genetic makeup of the host plant. NopP2 plays a crucial role in promoting nodulation in various V. radiata varieties. Additionally, NopP2 is essential for early infection and the formation of nodules in compatible plants. Through evolutionary analysis, we discovered that bradyrhizobial NopPs can be categorized into two distinct clusters: NopP1 and NopP2. Furthermore, both types of bradyrhizobial NopPs were conserved within their respective groups. Our findings suggest that NopP2 serves as a mechanism for optimizing the symbiotic relationship between V. radiata and B. elkanii USDA61 by interacting with the pathogenesis related-10 (PR10) protein and reducing effector-triggered immunity (ETI) responses.
Additional Links: PMID-39424841
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@article {pmid39424841,
year = {2024},
author = {Piromyou, P and Pruksametanan, N and Nguyen, HP and Songwattana, P and Wongdee, J and Nareephot, P and Greetatorn, T and Teamtisong, K and Tittabutr, P and Boonkerd, N and Sato, S and Boonchuen, P and Okazaki, S and Teaumroong, N},
title = {NopP2 effector of Bradyrhizobium elkanii USDA61 is a determinant of nodulation in Vigna radiata cultivars.},
journal = {Scientific reports},
volume = {14},
number = {1},
pages = {24541},
pmid = {39424841},
issn = {2045-2322},
support = {grant number N11A670769//JSPS-NRCT by National Research Council of Thailand/ ; B13F660055//NSRF via the Program Management Unit for Human Resources & Institutional Development, Research, and Innovation/ ; grant number 195582//Thailand Science Research and Innovation (TSRI) and National Science Research and Innovation Fund (NSRF)/ ; },
mesh = {*Bradyrhizobium/physiology/genetics ; *Symbiosis ; *Plant Root Nodulation ; *Vigna/microbiology/genetics ; Bacterial Proteins/metabolism/genetics ; Phylogeny ; Plant Proteins/genetics/metabolism ; },
abstract = {The symbiotic relationship between legumes and rhizobia is known to be influenced by specific rhizobial type III effectors (T3Es) in certain cases. In this study, we present evidence that the symbiosis between Vigna radiata and Bradyrhizobium elkanii USDA61 is controlled by a T3E called NopP2, and this interaction is highly dependent on the genetic makeup of the host plant. NopP2 plays a crucial role in promoting nodulation in various V. radiata varieties. Additionally, NopP2 is essential for early infection and the formation of nodules in compatible plants. Through evolutionary analysis, we discovered that bradyrhizobial NopPs can be categorized into two distinct clusters: NopP1 and NopP2. Furthermore, both types of bradyrhizobial NopPs were conserved within their respective groups. Our findings suggest that NopP2 serves as a mechanism for optimizing the symbiotic relationship between V. radiata and B. elkanii USDA61 by interacting with the pathogenesis related-10 (PR10) protein and reducing effector-triggered immunity (ETI) responses.},
}
MeSH Terms:
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*Bradyrhizobium/physiology/genetics
*Symbiosis
*Plant Root Nodulation
*Vigna/microbiology/genetics
Bacterial Proteins/metabolism/genetics
Phylogeny
Plant Proteins/genetics/metabolism
RevDate: 2024-10-22
CmpDate: 2024-10-18
Transcriptomes of soybean roots and nodules inoculated with Sinorhizobium fredii with NopP and NopI variants.
Scientific data, 11(1):1146.
The major crop, soybean, forms root nodules with symbiotic rhizobia, providing energy and carbon to the bacteria in exchange for bioavailable nitrogen. The relationship is host-specific and highly host-regulated to maximize energy efficiency. Symbiotic nitrogen fixation (SNF) is greener than synthetic fertilizer for replenishing soil fertility, contributing to yield increase. Nodulation Outer Protein P (NopP) and NopI of the type 3 secretion system (T3SS) of the rhizobium determine host specificity. Sinorhizobium fredii CCBAU25509 (R2) and CCBAU45436 (R4) have different NopP and NopI variants, affecting their respective symbiotic compatibilities with the cultivated soybean C08 and the wild soybean W05. Swapping the NopP variants between R2 and R4 has been shown to switch their compatibility with C08 with the rj2/Rfg1 genotype. To understand the effects of Nops on host compatibility, analyses on the transcriptomic data of W05 roots and nodules inoculated with S. fredii strains containing Nop variants uncovered many differentially expressed genes related to nodulation and nodule functions, providing important information on the effects of Nops on hosts and nodules.
Additional Links: PMID-39424807
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@article {pmid39424807,
year = {2024},
author = {Fan, K and Xiao, Z and Wang, L and Cheung, WL and Wong, FL and Zhang, F and Li, MW and Lam, HM},
title = {Transcriptomes of soybean roots and nodules inoculated with Sinorhizobium fredii with NopP and NopI variants.},
journal = {Scientific data},
volume = {11},
number = {1},
pages = {1146},
pmid = {39424807},
issn = {2052-4463},
mesh = {*Glycine max/microbiology/genetics ; *Sinorhizobium fredii/genetics ; *Symbiosis ; *Transcriptome ; *Bacterial Proteins/genetics ; *Root Nodules, Plant/microbiology ; *Plant Roots/microbiology ; Nitrogen Fixation/genetics ; },
abstract = {The major crop, soybean, forms root nodules with symbiotic rhizobia, providing energy and carbon to the bacteria in exchange for bioavailable nitrogen. The relationship is host-specific and highly host-regulated to maximize energy efficiency. Symbiotic nitrogen fixation (SNF) is greener than synthetic fertilizer for replenishing soil fertility, contributing to yield increase. Nodulation Outer Protein P (NopP) and NopI of the type 3 secretion system (T3SS) of the rhizobium determine host specificity. Sinorhizobium fredii CCBAU25509 (R2) and CCBAU45436 (R4) have different NopP and NopI variants, affecting their respective symbiotic compatibilities with the cultivated soybean C08 and the wild soybean W05. Swapping the NopP variants between R2 and R4 has been shown to switch their compatibility with C08 with the rj2/Rfg1 genotype. To understand the effects of Nops on host compatibility, analyses on the transcriptomic data of W05 roots and nodules inoculated with S. fredii strains containing Nop variants uncovered many differentially expressed genes related to nodulation and nodule functions, providing important information on the effects of Nops on hosts and nodules.},
}
MeSH Terms:
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*Glycine max/microbiology/genetics
*Sinorhizobium fredii/genetics
*Symbiosis
*Transcriptome
*Bacterial Proteins/genetics
*Root Nodules, Plant/microbiology
*Plant Roots/microbiology
Nitrogen Fixation/genetics
RevDate: 2024-10-18
Enhancement of PFAS Stress Tolerance and Wastewater Treatment Efficiency by Arbuscular Mycorrhizal Fungi in Constructed Wetlands.
Environmental research pii:S0013-9351(24)02055-3 [Epub ahead of print].
This study aims to explore the effects of arbuscular mycorrhizal fungi (AMF) on the growth of Iris pseudacorus L. and treatment efficacy in constructed wetlands (CWs) subjected to stress from per-and poly-fluoroalkyl substances (PFASs). The findings reveal that PFASs exposure induces oxidative damage and inhibits the growth of I. pseudacorus. However, AMF symbiosis enhances plant tolerance to PFAS stress by modulating oxidative responses. AMF treatment not only promoted plant growth but also improved photosynthetic efficiency under PFAS exposure. Compared to non-AMF treatment, those with AMF treatment exhibited significantly increased levels of peroxidases and antioxidant enzymes, including peroxidase and superoxide dismutase, along with a notable reduction in lipid peroxidation. Additionally, AM symbiosis markedly enhanced the efficacy of CWs in the remediation of wastewater under PFASs-induced stress, with removal efficiencies for COD, TP, TN, and NH4[+]-N increasing by 19-34%, 67-180%, 106-137%, and 25-95%, respectively, compared to the AMF- treatments. In addition, the metabolic pathways of PFASs appeared to be influenced by their carbon chain length, with long-chain PFASs like perfluorooctanoic acid (PFOA) and perfluoro anionic acid (PFNA) exhibiting more complex pathways compared to short-chain PFASs such as perfluoro acetic acid (PFPeA), and perfluoro hexanoic acid (PFHpA). These results suggest that AMF-plant symbiosis can enhance plant resilience against PFAS-induced stress and improve the pollutant removal efficiency of CWs. This study highlights the significant potential of AMF in enhancing environmental remediation strategies, providing new insights for the more effective management of PFAS-contaminated ecosystems.
Additional Links: PMID-39424036
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PubMed:
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@article {pmid39424036,
year = {2024},
author = {Wang, Y and Li, X and You, L and Hu, S and Fang, J and Hu, B and Chen, Z},
title = {Enhancement of PFAS Stress Tolerance and Wastewater Treatment Efficiency by Arbuscular Mycorrhizal Fungi in Constructed Wetlands.},
journal = {Environmental research},
volume = {},
number = {},
pages = {120148},
doi = {10.1016/j.envres.2024.120148},
pmid = {39424036},
issn = {1096-0953},
abstract = {This study aims to explore the effects of arbuscular mycorrhizal fungi (AMF) on the growth of Iris pseudacorus L. and treatment efficacy in constructed wetlands (CWs) subjected to stress from per-and poly-fluoroalkyl substances (PFASs). The findings reveal that PFASs exposure induces oxidative damage and inhibits the growth of I. pseudacorus. However, AMF symbiosis enhances plant tolerance to PFAS stress by modulating oxidative responses. AMF treatment not only promoted plant growth but also improved photosynthetic efficiency under PFAS exposure. Compared to non-AMF treatment, those with AMF treatment exhibited significantly increased levels of peroxidases and antioxidant enzymes, including peroxidase and superoxide dismutase, along with a notable reduction in lipid peroxidation. Additionally, AM symbiosis markedly enhanced the efficacy of CWs in the remediation of wastewater under PFASs-induced stress, with removal efficiencies for COD, TP, TN, and NH4[+]-N increasing by 19-34%, 67-180%, 106-137%, and 25-95%, respectively, compared to the AMF- treatments. In addition, the metabolic pathways of PFASs appeared to be influenced by their carbon chain length, with long-chain PFASs like perfluorooctanoic acid (PFOA) and perfluoro anionic acid (PFNA) exhibiting more complex pathways compared to short-chain PFASs such as perfluoro acetic acid (PFPeA), and perfluoro hexanoic acid (PFHpA). These results suggest that AMF-plant symbiosis can enhance plant resilience against PFAS-induced stress and improve the pollutant removal efficiency of CWs. This study highlights the significant potential of AMF in enhancing environmental remediation strategies, providing new insights for the more effective management of PFAS-contaminated ecosystems.},
}
RevDate: 2024-10-18
Tripartite interactions between grapevine, viruses, and arbuscular mycorrhizal fungi provide insights into modulation of oxidative stress responses.
Journal of plant physiology, 303:154372 pii:S0176-1617(24)00203-7 [Epub ahead of print].
Arbuscular mycorrhizal fungi (AMF) can be beneficial for plants exposed to abiotic and biotic stressors. Although widely present in agroecosystems, AMF influence on crop responses to virus infection is underexplored, particularly in woody plant species such as grapevine. Here, a two-year greenhouse experiment was set up to test the hypothesis that AMF alleviate virus-induced oxidative stress in grapevine. The 'Merlot' cultivar was infected with three grapevine-associated viruses and subsequently colonized with two AMF inocula, containing one or three species, respectively. Five and fifteen months after AMF inoculation, lipid peroxidation - LPO as an indicator of oxidative stress and indicators of antioxidative response (proline, ascorbate - AsA, superoxide dismutase - SOD, ascorbate- APX and guaiacol peroxidases - GPOD, polyphenol oxidase - PPO, glutathione reductase - GR) were analysed. Expression of genes coding for a stilbene synthase (STS1), an enhanced disease susceptibility (EDS1) and a lipoxygenase (LOX) were determined in the second harvesting. AMF induced reduction of AsA and SOD over both years, which, combined with not AMF-triggered APX and GR, suggests decreased activation of the ascorbate-glutathione cycle. In the mature phase of the AM symbiosis establishment GPOD emerged as an important mechanism for scavenging H2O2 accumulation. These results, together with reduction in STS1 and increase in EDS1 gene expression, suggest more efficient reactive oxygen species scavenging in plants inoculated with AMF. Composition of AMF inocula was important for proline accumulation. Overall, our study improves the knowledge on ubiquitous grapevine-virus-AMF systems in the field, highlighting that established functional AM symbiosis could reduce virus-induced stress.
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@article {pmid39423687,
year = {2024},
author = {Radić, T and Vuković, R and Gaši, E and Kujundžić, D and Čarija, M and Balestrini, R and Sillo, F and Gambino, G and Hančević, K},
title = {Tripartite interactions between grapevine, viruses, and arbuscular mycorrhizal fungi provide insights into modulation of oxidative stress responses.},
journal = {Journal of plant physiology},
volume = {303},
number = {},
pages = {154372},
doi = {10.1016/j.jplph.2024.154372},
pmid = {39423687},
issn = {1618-1328},
abstract = {Arbuscular mycorrhizal fungi (AMF) can be beneficial for plants exposed to abiotic and biotic stressors. Although widely present in agroecosystems, AMF influence on crop responses to virus infection is underexplored, particularly in woody plant species such as grapevine. Here, a two-year greenhouse experiment was set up to test the hypothesis that AMF alleviate virus-induced oxidative stress in grapevine. The 'Merlot' cultivar was infected with three grapevine-associated viruses and subsequently colonized with two AMF inocula, containing one or three species, respectively. Five and fifteen months after AMF inoculation, lipid peroxidation - LPO as an indicator of oxidative stress and indicators of antioxidative response (proline, ascorbate - AsA, superoxide dismutase - SOD, ascorbate- APX and guaiacol peroxidases - GPOD, polyphenol oxidase - PPO, glutathione reductase - GR) were analysed. Expression of genes coding for a stilbene synthase (STS1), an enhanced disease susceptibility (EDS1) and a lipoxygenase (LOX) were determined in the second harvesting. AMF induced reduction of AsA and SOD over both years, which, combined with not AMF-triggered APX and GR, suggests decreased activation of the ascorbate-glutathione cycle. In the mature phase of the AM symbiosis establishment GPOD emerged as an important mechanism for scavenging H2O2 accumulation. These results, together with reduction in STS1 and increase in EDS1 gene expression, suggest more efficient reactive oxygen species scavenging in plants inoculated with AMF. Composition of AMF inocula was important for proline accumulation. Overall, our study improves the knowledge on ubiquitous grapevine-virus-AMF systems in the field, highlighting that established functional AM symbiosis could reduce virus-induced stress.},
}
RevDate: 2024-10-18
It's complicated: relationships between integrative and conjugative elements and their bacterial hosts.
Current opinion in microbiology, 82:102556 pii:S1369-5274(24)00132-2 [Epub ahead of print].
Integrative and conjugative elements (ICEs) are typically found integrated in a bacterial host chromosome. They can excise, replicate, and transfer from cell to cell. Many contain genes that confer phenotypes to host cells, including antibiotic resistances, specialized metabolisms, phage defense, and symbiosis or pathogenesis determinants. Recent studies revealed that at least three ICEs (ICEclc, Tn916, and TnSmu1) cause growth arrest or death of host cells upon element activation. This review highlights the complex interactions between ICEs and their hosts, including the recent examples of the significant costs to host cells. We contrast two examples of killing, ICEclc and Tn916, in which killing, respectively, benefits or impairs conjugation and emphasize the importance of understanding the impacts of ICE-host relationships on conjugation. ICEs are typically only active in a small fraction of cells in a population, and we discuss how phenotypes normally occurring in a small subset of host cells can be uncovered.
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@article {pmid39423563,
year = {2024},
author = {Gomberg, AF and Grossman, AD},
title = {It's complicated: relationships between integrative and conjugative elements and their bacterial hosts.},
journal = {Current opinion in microbiology},
volume = {82},
number = {},
pages = {102556},
doi = {10.1016/j.mib.2024.102556},
pmid = {39423563},
issn = {1879-0364},
abstract = {Integrative and conjugative elements (ICEs) are typically found integrated in a bacterial host chromosome. They can excise, replicate, and transfer from cell to cell. Many contain genes that confer phenotypes to host cells, including antibiotic resistances, specialized metabolisms, phage defense, and symbiosis or pathogenesis determinants. Recent studies revealed that at least three ICEs (ICEclc, Tn916, and TnSmu1) cause growth arrest or death of host cells upon element activation. This review highlights the complex interactions between ICEs and their hosts, including the recent examples of the significant costs to host cells. We contrast two examples of killing, ICEclc and Tn916, in which killing, respectively, benefits or impairs conjugation and emphasize the importance of understanding the impacts of ICE-host relationships on conjugation. ICEs are typically only active in a small fraction of cells in a population, and we discuss how phenotypes normally occurring in a small subset of host cells can be uncovered.},
}
RevDate: 2024-10-18
Effect of the drying methods on the stabilization of symbiotic microbeads produced by ionic gelation.
Food chemistry, 464(Pt 1):141546 pii:S0308-8146(24)03196-0 [Epub ahead of print].
Lactobacillus salivarius was encapsulated by ionic gelation using high polymerization degree agave fructans and sodium alginate to obtain symbiotic microspheres stabilized by different drying methods. The microbeads were characterized by physicochemical, reconstitution, microstructure, thermal, radiographic and infrared properties. The viability of L. salivarius was evaluated for one month at 4 °C and under in vitro gastrointestinal conditions. The ionic gelation-drying technique allowed an encapsulation efficiency greater than 90 %. Oven-dried microbeads showed higher viability under in vitro gastrointestinal conditions. Freeze-drying microbeads showed higher viability during storage at 4 °C, and spray-dried microbeads showed a more notable survival at 40 °C after one month of storage. All samples showed up to 7 log CFU/g of viability after in vitro digestion, which is a benefit for consumer health. It was concluded that the ionic gelation-drying process allows obtaining dry microbeads with the potential to be added to various food products.
Additional Links: PMID-39423537
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PubMed:
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@article {pmid39423537,
year = {2024},
author = {Juárez-Trujillo, N and Ortiz-Basurto, RI and Chacón-López, MA and Martinez-Gutierrez, F and Pascual-Pineda, LA and Montalvo-González, E and Jiménez-Fernández, M},
title = {Effect of the drying methods on the stabilization of symbiotic microbeads produced by ionic gelation.},
journal = {Food chemistry},
volume = {464},
number = {Pt 1},
pages = {141546},
doi = {10.1016/j.foodchem.2024.141546},
pmid = {39423537},
issn = {1873-7072},
abstract = {Lactobacillus salivarius was encapsulated by ionic gelation using high polymerization degree agave fructans and sodium alginate to obtain symbiotic microspheres stabilized by different drying methods. The microbeads were characterized by physicochemical, reconstitution, microstructure, thermal, radiographic and infrared properties. The viability of L. salivarius was evaluated for one month at 4 °C and under in vitro gastrointestinal conditions. The ionic gelation-drying technique allowed an encapsulation efficiency greater than 90 %. Oven-dried microbeads showed higher viability under in vitro gastrointestinal conditions. Freeze-drying microbeads showed higher viability during storage at 4 °C, and spray-dried microbeads showed a more notable survival at 40 °C after one month of storage. All samples showed up to 7 log CFU/g of viability after in vitro digestion, which is a benefit for consumer health. It was concluded that the ionic gelation-drying process allows obtaining dry microbeads with the potential to be added to various food products.},
}
RevDate: 2024-10-18
Multi-omics analysis reveals the interplay between pulmonary microbiome and host in immunocompromised patients with sepsis-induced acute lung injury.
Microbiology spectrum [Epub ahead of print].
UNLABELLED: The mechanisms behind the high inflammatory state and immunocompromise in severe sepsis remain unclear. While microbiota's role in immune regulation is known, the impact of pulmonary microbiota on sepsis progression is not fully understood. This study aims to investigate pulmonary microbial characteristics in septic patients and their relationship with host immune-related genes and clinical features. Fifty-four sepsis patients were divided into the immunocompromised host (ICH) group (n = 18) and the control group (n = 36). Bronchoalveolar lavage fluid (BALF) was analyzed using metagenomic next-generation sequencing (mNGS) to assess the pulmonary microbiome, and transcriptomic sequencing evaluated host gene expression. The pulmonary microbiota network in the ICH group showed notable alterations. Symbiotic bacteria like Streptococcus salivarius and Streptococcus oralis were key taxa in the control group. In contrast, opportunistic pathogens such as Campylobacter concisus and Prevotella melaninogenica, typically linked to infections in various body sites, dominated in the ICH group. Transcriptomic analysis revealed differential genes between the two groups. The downregulated differential genes in the ICH group were primarily enriched in pathways related to T-cell activation and the Type I interferon signaling pathway, both crucial for the immune system. Further correlation analysis identified significant associations between certain microbes and host genes, as well as clinical indicators, particularly with species like Campylobacter concisus, Streptococcus salivarius, Streptococcus oralis, and several species of Veillonella. These findings suggest that alterations in the pulmonary microbiome, especially the presence of opportunistic pathogens, may contribute to immune dysregulation in immunocompromised septic patients, warranting further research to explore causal relationships.
IMPORTANCE: Recent research has substantiated the significant role of microbiota in immune regulation, which could influence high inflammatory state and immunocompromise in patients with severe sepsis, as well as provide new opportunities for acute lung injury induced by sepsis diagnosis and treatment. Our study identified some potential critical microbes (Campylobacter concisus and several species of Veillonella), which were correlated with immune-related genes and might be the novel target to regulate immunotherapy in sepsis.
Additional Links: PMID-39422492
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PubMed:
Citation:
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@article {pmid39422492,
year = {2024},
author = {Lu, F and Huang, T and Chen, R and Yin, H},
title = {Multi-omics analysis reveals the interplay between pulmonary microbiome and host in immunocompromised patients with sepsis-induced acute lung injury.},
journal = {Microbiology spectrum},
volume = {},
number = {},
pages = {e0142424},
doi = {10.1128/spectrum.01424-24},
pmid = {39422492},
issn = {2165-0497},
abstract = {UNLABELLED: The mechanisms behind the high inflammatory state and immunocompromise in severe sepsis remain unclear. While microbiota's role in immune regulation is known, the impact of pulmonary microbiota on sepsis progression is not fully understood. This study aims to investigate pulmonary microbial characteristics in septic patients and their relationship with host immune-related genes and clinical features. Fifty-four sepsis patients were divided into the immunocompromised host (ICH) group (n = 18) and the control group (n = 36). Bronchoalveolar lavage fluid (BALF) was analyzed using metagenomic next-generation sequencing (mNGS) to assess the pulmonary microbiome, and transcriptomic sequencing evaluated host gene expression. The pulmonary microbiota network in the ICH group showed notable alterations. Symbiotic bacteria like Streptococcus salivarius and Streptococcus oralis were key taxa in the control group. In contrast, opportunistic pathogens such as Campylobacter concisus and Prevotella melaninogenica, typically linked to infections in various body sites, dominated in the ICH group. Transcriptomic analysis revealed differential genes between the two groups. The downregulated differential genes in the ICH group were primarily enriched in pathways related to T-cell activation and the Type I interferon signaling pathway, both crucial for the immune system. Further correlation analysis identified significant associations between certain microbes and host genes, as well as clinical indicators, particularly with species like Campylobacter concisus, Streptococcus salivarius, Streptococcus oralis, and several species of Veillonella. These findings suggest that alterations in the pulmonary microbiome, especially the presence of opportunistic pathogens, may contribute to immune dysregulation in immunocompromised septic patients, warranting further research to explore causal relationships.
IMPORTANCE: Recent research has substantiated the significant role of microbiota in immune regulation, which could influence high inflammatory state and immunocompromise in patients with severe sepsis, as well as provide new opportunities for acute lung injury induced by sepsis diagnosis and treatment. Our study identified some potential critical microbes (Campylobacter concisus and several species of Veillonella), which were correlated with immune-related genes and might be the novel target to regulate immunotherapy in sepsis.},
}
RevDate: 2024-10-18
Unveiling symbiotic bacterial communities in insects feeding on the latex-rich plant Ficus microcarpa.
Bulletin of entomological research pii:S0007485324000439 [Epub ahead of print].
The diversity and health of insects that feed on plants are closely related to their mutualistic symbionts and host plants. These symbiotic partners significantly influence various metabolic activities in these insects. However, the symbiotic bacterial community of toxic plant feeders still needs further characterisation. This study aims to unravel bacterial communities associated with the different species of insect representing three insect orders: Thysanoptera, Hemiptera, and Lepidoptera, along with their predicted functional role, which exclusively feeds on latex-rich plant species Ficus microcarpa. By using 16S rRNA gene high-throughput sequencing, the analysis was able to define the major alignment of the bacterial population, primarily comprising Proteobacteria, Firmicutes, Bacteroidota, Actinobacteriota, and Acidobacteriota. Significant differences in symbiotic organisms between three insect groups were discovered by the study: hemipterans had Burkholderia and Buchnera, and lepidopterans had Acinetobacter. At the same time, Pseudomonas was detected in high abundance in both lepidopteran and thysanopteran insects. Furthermore, these symbionts exhibit consistent core functions, potentially explaining how different insects can consume the same host plant. The identified core functions of symbionts open avenues for innovative approaches in utilising these relationships to develop environment-friendly solutions for pest control, with broader implications for agriculture and environmental conservation.
Additional Links: PMID-39421892
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PubMed:
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@article {pmid39421892,
year = {2024},
author = {Naveed, WA and Liu, Q and Lu, C and Huang, X},
title = {Unveiling symbiotic bacterial communities in insects feeding on the latex-rich plant Ficus microcarpa.},
journal = {Bulletin of entomological research},
volume = {},
number = {},
pages = {1-11},
doi = {10.1017/S0007485324000439},
pmid = {39421892},
issn = {1475-2670},
abstract = {The diversity and health of insects that feed on plants are closely related to their mutualistic symbionts and host plants. These symbiotic partners significantly influence various metabolic activities in these insects. However, the symbiotic bacterial community of toxic plant feeders still needs further characterisation. This study aims to unravel bacterial communities associated with the different species of insect representing three insect orders: Thysanoptera, Hemiptera, and Lepidoptera, along with their predicted functional role, which exclusively feeds on latex-rich plant species Ficus microcarpa. By using 16S rRNA gene high-throughput sequencing, the analysis was able to define the major alignment of the bacterial population, primarily comprising Proteobacteria, Firmicutes, Bacteroidota, Actinobacteriota, and Acidobacteriota. Significant differences in symbiotic organisms between three insect groups were discovered by the study: hemipterans had Burkholderia and Buchnera, and lepidopterans had Acinetobacter. At the same time, Pseudomonas was detected in high abundance in both lepidopteran and thysanopteran insects. Furthermore, these symbionts exhibit consistent core functions, potentially explaining how different insects can consume the same host plant. The identified core functions of symbionts open avenues for innovative approaches in utilising these relationships to develop environment-friendly solutions for pest control, with broader implications for agriculture and environmental conservation.},
}
RevDate: 2024-10-19
The rootstock genotype shapes the diversity of pecan (Carya illinoinensis) rhizosphere microbial community.
Frontiers in microbiology, 15:1461685.
Pecans (Carya illinoinensis), one of the most valuable native North American nut crops, are commonly propagated through grafting to preserve the desired characteristics from parent trees. Since successful cultivation of pecan trees relies on the interplay among scion varieties, rootstocks, and soil conditions, this study investigated the microbial change to communities in the soils and roots of southern (87MX5-1.7) and northern (Peruque) rootstocks in a rootstock test orchard. Both grafted with the 'Pawnee' scion cultivar. Bacterial 16S ribosomal RNA and fungal ITS were amplified from both roots and rhizosphere soils of the two 10-year-grafted trees, then sequenced and annotated into trophic and nutrient-related groups to characterize the rhizosphere microbiota. The Peruque roots had a higher relative abundance of saprotroph fungi, while 87MX5-1.7 exhibited higher levels of symbiotroph fungi and nitrogen fixation-related bacteria. Among them, the presence of symbiotroph fungi, particularly ectomycorrhizal fungi, notably differed between these two rootstocks, with a significantly higher presence observed in the root of 87MX5-1.7 compared to Peruque. This variation likely leads to divergent pathways of nutrient translocation: Peruque was in favor of multiple fungi (Russula and Inocybe) to gain nutrition, while 87MX5-1.7 preferred a specific domain of fungi (Tuber) and nitrogen fixation-related bacteria (Bradyrhizobia) to form beneficial symbiosis. Moreover, the presence of pathogens suggested a potential risk of Fusarium patch and snow molds in 87MX5-1.7, while canker and black foot disease pose threats in Peruque. The findings of this study suggest that rootstocks from different origins shape rhizosphere microbes differently, potentially affecting nutrient uptake and nut yield. Exploring rootstock-microbe combinations could provide insights into optimizing scion growth and ultimately increasing nut yield. By understanding how different rootstock-microbe interactions influence pecan tree development, growers can strategically select combinations that promote beneficial symbiotic relationships, enhancing nutrient uptake, disease resistance, and overall tree vigor.
Additional Links: PMID-39421556
PubMed:
Citation:
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@article {pmid39421556,
year = {2024},
author = {Ren, W and Zhang, L and Tondre, B and Wang, X and Xu, T},
title = {The rootstock genotype shapes the diversity of pecan (Carya illinoinensis) rhizosphere microbial community.},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1461685},
pmid = {39421556},
issn = {1664-302X},
abstract = {Pecans (Carya illinoinensis), one of the most valuable native North American nut crops, are commonly propagated through grafting to preserve the desired characteristics from parent trees. Since successful cultivation of pecan trees relies on the interplay among scion varieties, rootstocks, and soil conditions, this study investigated the microbial change to communities in the soils and roots of southern (87MX5-1.7) and northern (Peruque) rootstocks in a rootstock test orchard. Both grafted with the 'Pawnee' scion cultivar. Bacterial 16S ribosomal RNA and fungal ITS were amplified from both roots and rhizosphere soils of the two 10-year-grafted trees, then sequenced and annotated into trophic and nutrient-related groups to characterize the rhizosphere microbiota. The Peruque roots had a higher relative abundance of saprotroph fungi, while 87MX5-1.7 exhibited higher levels of symbiotroph fungi and nitrogen fixation-related bacteria. Among them, the presence of symbiotroph fungi, particularly ectomycorrhizal fungi, notably differed between these two rootstocks, with a significantly higher presence observed in the root of 87MX5-1.7 compared to Peruque. This variation likely leads to divergent pathways of nutrient translocation: Peruque was in favor of multiple fungi (Russula and Inocybe) to gain nutrition, while 87MX5-1.7 preferred a specific domain of fungi (Tuber) and nitrogen fixation-related bacteria (Bradyrhizobia) to form beneficial symbiosis. Moreover, the presence of pathogens suggested a potential risk of Fusarium patch and snow molds in 87MX5-1.7, while canker and black foot disease pose threats in Peruque. The findings of this study suggest that rootstocks from different origins shape rhizosphere microbes differently, potentially affecting nutrient uptake and nut yield. Exploring rootstock-microbe combinations could provide insights into optimizing scion growth and ultimately increasing nut yield. By understanding how different rootstock-microbe interactions influence pecan tree development, growers can strategically select combinations that promote beneficial symbiotic relationships, enhancing nutrient uptake, disease resistance, and overall tree vigor.},
}
RevDate: 2024-10-18
Peptide Signals Regulate Nitrogen Deficiency Adaptation of Dicotyledonous Model Plants.
Plant, cell & environment [Epub ahead of print].
Nitrogen is a crucial macroelement essential for plant growth and development. In Arabidopsis Thaliana, classical phytohormones such as auxin and cytokinin orchestrate local and systemic signalling networks coordinate plant growth and development in response to nitrogen deficiency. Nowadays, emerging signalling pathways involving small peptides like CLAVATA3/EMBRYO SURROUNDINGR REGION (CLE) and C-TERMINALLY ENCODED PEPTIDE (CEP) and their corresponding kinase receptors, also regulate Arabidopsis' adaptation to nitrogen scarcity. Unlike Arabidopsis, which adapts to nitrogen deficiency by changing root development, legumes have the unique ability to form nitrogen-fixing root nodules through symbiotic interactions with soil rhizobia. During the symbiotic nodulation in Medicago, CLE and CEP peptides and their receptors consist of an autoregulatory network governing the number of nodules in accordance with the soil nitrogen level. Additionally, other plant peptides, such as phytosulfokine (PSK) and root meristem growth factors (RGF), have been identified as new regulators of leguminous root nodule development under nitrogen-limited condition. However, the precise mechanism by which these peptides coordinate nitrogen deficiency response and the development of nitrogen-fixing organs remains to be fully elucidated. This review summarises the adaptive strategies of dicotyledons to nitrogen deficiency, with a particular focus on the regulation of Medicago nitrogen-fixing nodule development by the peptides.
Additional Links: PMID-39420598
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PubMed:
Citation:
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@article {pmid39420598,
year = {2024},
author = {Luo, L and Yu, L and Yang, J and Wang, E},
title = {Peptide Signals Regulate Nitrogen Deficiency Adaptation of Dicotyledonous Model Plants.},
journal = {Plant, cell & environment},
volume = {},
number = {},
pages = {},
doi = {10.1111/pce.15203},
pmid = {39420598},
issn = {1365-3040},
abstract = {Nitrogen is a crucial macroelement essential for plant growth and development. In Arabidopsis Thaliana, classical phytohormones such as auxin and cytokinin orchestrate local and systemic signalling networks coordinate plant growth and development in response to nitrogen deficiency. Nowadays, emerging signalling pathways involving small peptides like CLAVATA3/EMBRYO SURROUNDINGR REGION (CLE) and C-TERMINALLY ENCODED PEPTIDE (CEP) and their corresponding kinase receptors, also regulate Arabidopsis' adaptation to nitrogen scarcity. Unlike Arabidopsis, which adapts to nitrogen deficiency by changing root development, legumes have the unique ability to form nitrogen-fixing root nodules through symbiotic interactions with soil rhizobia. During the symbiotic nodulation in Medicago, CLE and CEP peptides and their receptors consist of an autoregulatory network governing the number of nodules in accordance with the soil nitrogen level. Additionally, other plant peptides, such as phytosulfokine (PSK) and root meristem growth factors (RGF), have been identified as new regulators of leguminous root nodule development under nitrogen-limited condition. However, the precise mechanism by which these peptides coordinate nitrogen deficiency response and the development of nitrogen-fixing organs remains to be fully elucidated. This review summarises the adaptive strategies of dicotyledons to nitrogen deficiency, with a particular focus on the regulation of Medicago nitrogen-fixing nodule development by the peptides.},
}
RevDate: 2024-10-19
CmpDate: 2024-10-18
Widespread production of plant growth-promoting hormones among marine bacteria and their impacts on the growth of a marine diatom.
Microbiome, 12(1):205.
BACKGROUND: Reciprocal exchanges of metabolites between phytoplankton and bacteria influence the fitness of these microorganisms which ultimately shapes the productivity of marine ecosystems. Recent evidence suggests that plant growth-promoting hormones may be key metabolites within mutualistic phytoplankton-bacteria partnerships, but very little is known about the diversity of plant growth-promoting hormones produced by marine bacteria and their specific effects on phytoplankton growth. Here, we aimed to investigate the capacity of marine bacteria to produce 7 plant growth-promoting hormones and the effects of these hormones on Actinocyclus sp. growth.
RESULTS: We examined the plant growth-promoting hormone synthesis capabilities of 14 bacterial strains that enhance the growth of the common diatom Actinocyclus. Plant growth-promoting hormone biosynthesis was ubiquitous among the bacteria tested. Indeed all 14 strains displayed the genomic potential to synthesise multiple hormones, and mass-spectrometry confirmed that each strain produced at least 6 out of the 7 tested plant growth-promoting hormones. Some of the plant growth-promoting hormones identified here, such as brassinolide and trans-zeatin, have never been reported in marine microorganisms. Importantly, all strains produced the hormone indole-3 acetic acid (IAA) in high concentrations and released it into their surroundings. Furthermore, indole-3 acetic acid extracellular concentrations were positively correlated with the ability of each strain to promote Actinocyclus growth. When inoculated with axenic Actinocyclus cultures, only indole-3 acetic acid and gibberellic acid enhanced the growth of the diatom, with cultures exposed to indole-3 acetic acid exhibiting a two-fold increase in cell numbers.
CONCLUSION: Our results reveal that marine bacteria produce a much broader range of plant growth-promoting hormones than previously suspected and that some of these compounds enhance the growth of a marine diatom. These findings suggest plant growth-promoting hormones play a large role in microbial communication and broaden our knowledge of their fuctions in the marine environment. Video Abstract.
Additional Links: PMID-39420440
PubMed:
Citation:
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@article {pmid39420440,
year = {2024},
author = {Khalil, A and Bramucci, AR and Focardi, A and Le Reun, N and Willams, NLR and Kuzhiumparambil, U and Raina, JB and Seymour, JR},
title = {Widespread production of plant growth-promoting hormones among marine bacteria and their impacts on the growth of a marine diatom.},
journal = {Microbiome},
volume = {12},
number = {1},
pages = {205},
pmid = {39420440},
issn = {2049-2618},
support = {DP180100838//Australian Research Council Grant/ ; DP180100838//Australian Research Council Grant/ ; FT210100100//Australian Research Council Grant/ ; DP180100838//Australian Research Council Grant/ ; },
mesh = {*Plant Growth Regulators/metabolism ; *Diatoms/growth & development/metabolism ; *Bacteria/metabolism/classification/genetics ; Indoleacetic Acids/metabolism ; Seawater/microbiology ; Phytoplankton/growth & development/metabolism ; Aquatic Organisms/metabolism/growth & development ; Symbiosis ; },
abstract = {BACKGROUND: Reciprocal exchanges of metabolites between phytoplankton and bacteria influence the fitness of these microorganisms which ultimately shapes the productivity of marine ecosystems. Recent evidence suggests that plant growth-promoting hormones may be key metabolites within mutualistic phytoplankton-bacteria partnerships, but very little is known about the diversity of plant growth-promoting hormones produced by marine bacteria and their specific effects on phytoplankton growth. Here, we aimed to investigate the capacity of marine bacteria to produce 7 plant growth-promoting hormones and the effects of these hormones on Actinocyclus sp. growth.
RESULTS: We examined the plant growth-promoting hormone synthesis capabilities of 14 bacterial strains that enhance the growth of the common diatom Actinocyclus. Plant growth-promoting hormone biosynthesis was ubiquitous among the bacteria tested. Indeed all 14 strains displayed the genomic potential to synthesise multiple hormones, and mass-spectrometry confirmed that each strain produced at least 6 out of the 7 tested plant growth-promoting hormones. Some of the plant growth-promoting hormones identified here, such as brassinolide and trans-zeatin, have never been reported in marine microorganisms. Importantly, all strains produced the hormone indole-3 acetic acid (IAA) in high concentrations and released it into their surroundings. Furthermore, indole-3 acetic acid extracellular concentrations were positively correlated with the ability of each strain to promote Actinocyclus growth. When inoculated with axenic Actinocyclus cultures, only indole-3 acetic acid and gibberellic acid enhanced the growth of the diatom, with cultures exposed to indole-3 acetic acid exhibiting a two-fold increase in cell numbers.
CONCLUSION: Our results reveal that marine bacteria produce a much broader range of plant growth-promoting hormones than previously suspected and that some of these compounds enhance the growth of a marine diatom. These findings suggest plant growth-promoting hormones play a large role in microbial communication and broaden our knowledge of their fuctions in the marine environment. Video Abstract.},
}
MeSH Terms:
show MeSH Terms
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*Plant Growth Regulators/metabolism
*Diatoms/growth & development/metabolism
*Bacteria/metabolism/classification/genetics
Indoleacetic Acids/metabolism
Seawater/microbiology
Phytoplankton/growth & development/metabolism
Aquatic Organisms/metabolism/growth & development
Symbiosis
RevDate: 2024-10-17
Asaia spp. Accelerate development of the yellow fever mosquito, Aedes aegypti, via interactions with the vertically transmitted larval microbiome.
Journal of applied microbiology pii:7825875 [Epub ahead of print].
AIMS: A wide range of vector control programs rely on the efficient production and release of male mosquito. Asaia bacteria are described as potential symbionts of several mosquito species but their relationship with Aedes aegypti has never been rigorously tested. Here we aimed to quantify the benefits of three Asaia species on host development in Ae. aegypti, and the ability of these bacteria to form a stable symbiotic association with growing larvae.
METHODS AND RESULTS: In order to disentangle direct and indirect effects of Asaia inoculation on host development, experiments used insects with an intact microbiome and those reared in near-aseptic conditions, while we characterized bacterial communities and Asaia densities with culture dependent and independent methods (16S rRNA amplicon sequencing). Neonate larvae were inoculated with Asaia spp. for 24 hours, or left as uninoculated controls, all were reared on sterile food. Aseptic larvae were produced by surface sterilization of eggs. Although all Asaia were transient members of the gut community, two species accelerated larval development relative to controls. The two mutualistic species had lasting impacts on the larval microbiome, largely by altering the relative abundance of dominant bacteria, namely Klebsiella and Pseudomonas. Axenic larvae were dominated by Asaia when inoculated with this species but showed slower development than conventionally reared insects, indicating that Asaia alone could not restore normal development.
CONCLUSIONS: Our results reveal Asaia as a poor mutualist for Ae. aegypti, but with a species-specific positive effect on improving host performance mediated by interactions with other bacteria.
Additional Links: PMID-39419784
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PubMed:
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@article {pmid39419784,
year = {2024},
author = {Roman, A and Koenraadt, CJM and Raymond, B},
title = {Asaia spp. Accelerate development of the yellow fever mosquito, Aedes aegypti, via interactions with the vertically transmitted larval microbiome.},
journal = {Journal of applied microbiology},
volume = {},
number = {},
pages = {},
doi = {10.1093/jambio/lxae261},
pmid = {39419784},
issn = {1365-2672},
abstract = {AIMS: A wide range of vector control programs rely on the efficient production and release of male mosquito. Asaia bacteria are described as potential symbionts of several mosquito species but their relationship with Aedes aegypti has never been rigorously tested. Here we aimed to quantify the benefits of three Asaia species on host development in Ae. aegypti, and the ability of these bacteria to form a stable symbiotic association with growing larvae.
METHODS AND RESULTS: In order to disentangle direct and indirect effects of Asaia inoculation on host development, experiments used insects with an intact microbiome and those reared in near-aseptic conditions, while we characterized bacterial communities and Asaia densities with culture dependent and independent methods (16S rRNA amplicon sequencing). Neonate larvae were inoculated with Asaia spp. for 24 hours, or left as uninoculated controls, all were reared on sterile food. Aseptic larvae were produced by surface sterilization of eggs. Although all Asaia were transient members of the gut community, two species accelerated larval development relative to controls. The two mutualistic species had lasting impacts on the larval microbiome, largely by altering the relative abundance of dominant bacteria, namely Klebsiella and Pseudomonas. Axenic larvae were dominated by Asaia when inoculated with this species but showed slower development than conventionally reared insects, indicating that Asaia alone could not restore normal development.
CONCLUSIONS: Our results reveal Asaia as a poor mutualist for Ae. aegypti, but with a species-specific positive effect on improving host performance mediated by interactions with other bacteria.},
}
RevDate: 2024-10-17
Targeting Inflammation and Gut Microbiota with Antibacterial Therapy: Implications for Central Nervous System Health.
Ageing research reviews pii:S1568-1637(24)00362-3 [Epub ahead of print].
The complex symbiotic relationship between inflammation, the gut microbiota, and the central nervous system (CNS) has become a pivotal focus of contemporary biomedical research. Inflammation, as a physiological defense mechanism, plays a dual role as both a protective and pathological factor, and is intricately associated with gut microbiota homeostasis, often termed the "second brain." The gutbrain axis (GBA) exemplifies this multifaceted interaction, where gut health exerts significantly regulatory effects on CNS functions. Antibacterial therapies represent both promising and challenging strategies for modulating inflammation and gut microbiota composition to confer CNS benefits. However, while such therapies may exert positive modulatory effects on the gut microbiota, they also carry the potential to disrupt microbial equilibrium, potentially exacerbating neurological dysfunction. Recent advances have provided critical insights into the therapeutic implications of antibacterial interventions; nevertheless, the application of these therapies in the context of CNS health warrants a judicious and evidence-based approach. As research progresses, deeper investigation into the microbial-neural interface is essential to fully realize the potential of therapies targeting inflammation and the gut microbiota for CNS health. Future efforts should focus on refining antibacterial interventions to modulate the gut microbiota while minimizing disruption to microbial balance, thereby reducing risks and enhancing efficacy in CNS-related conditions. In conclusion, despite challenges, a more comprehensive understanding of the GBA, along with precise modulation through targeted antibacterial therapies, offers significant promise for advancing CNS disorder treatment. Continued research in this area will lead to innovative interventions and improved patient outcomes.
Additional Links: PMID-39419400
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PubMed:
Citation:
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@article {pmid39419400,
year = {2024},
author = {Wei, J and Liu, C and Qin, D and Ren, F and Duan, J and Chen, T and Wu, A},
title = {Targeting Inflammation and Gut Microbiota with Antibacterial Therapy: Implications for Central Nervous System Health.},
journal = {Ageing research reviews},
volume = {},
number = {},
pages = {102544},
doi = {10.1016/j.arr.2024.102544},
pmid = {39419400},
issn = {1872-9649},
abstract = {The complex symbiotic relationship between inflammation, the gut microbiota, and the central nervous system (CNS) has become a pivotal focus of contemporary biomedical research. Inflammation, as a physiological defense mechanism, plays a dual role as both a protective and pathological factor, and is intricately associated with gut microbiota homeostasis, often termed the "second brain." The gutbrain axis (GBA) exemplifies this multifaceted interaction, where gut health exerts significantly regulatory effects on CNS functions. Antibacterial therapies represent both promising and challenging strategies for modulating inflammation and gut microbiota composition to confer CNS benefits. However, while such therapies may exert positive modulatory effects on the gut microbiota, they also carry the potential to disrupt microbial equilibrium, potentially exacerbating neurological dysfunction. Recent advances have provided critical insights into the therapeutic implications of antibacterial interventions; nevertheless, the application of these therapies in the context of CNS health warrants a judicious and evidence-based approach. As research progresses, deeper investigation into the microbial-neural interface is essential to fully realize the potential of therapies targeting inflammation and the gut microbiota for CNS health. Future efforts should focus on refining antibacterial interventions to modulate the gut microbiota while minimizing disruption to microbial balance, thereby reducing risks and enhancing efficacy in CNS-related conditions. In conclusion, despite challenges, a more comprehensive understanding of the GBA, along with precise modulation through targeted antibacterial therapies, offers significant promise for advancing CNS disorder treatment. Continued research in this area will lead to innovative interventions and improved patient outcomes.},
}
RevDate: 2024-10-19
Symbiotic conserved arbuscular mycorrhiza fungi supports plant health.
The Science of the total environment, 955:176974 pii:S0048-9697(24)07131-6 [Epub ahead of print].
Arbuscular mycorrhiza fungi (AMF) forms a multi-beneficial symbiotic relationship with the host plant, therefore it is considered to be an effective helper to promote plant health. However, failure to consider the source or universality of AMF is often unstable during application. Therefore, it is necessary to screen potential AMF inoculants based on the source and the relationship with host. In search of more effective and broad-spectrum AMF inoculants, we studied AMF community structure properties of healthy and diseased plants in 24 fields from four sampling sites. The results indicated that the environmental filtering effect of roots was obvious, which was manifested as a decrease of α-diversity from rhizosphere to root. Differences in α-diversity between healthy and diseased roots further indicate the importance of AMF communities within roots for maintaining plant health. Glomus is significantly enriched and dominant in healthy roots, independent of environment and phylogenically conserved. Spores were further isolated and evaluated for their disease-preventing and pro-growth properties. Based on whether they were symbiotic with plant and root-enrichment characteristics, isolated AMF spores were classified as symbiotic conserved, symbiotic non-conserved, and non-symbiotic AMF. After spores were propagated and inoculated to plant roots, only symbiotic conserved AMF significantly promoted plant growth and maintained health, highlighting the potential of symbiotic conserved AMF in sustainable plant production.
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@article {pmid39419224,
year = {2024},
author = {Zhou, Y and Jin, Z and Ren, X and Hong, C and Hua, Z and Zhu, Y and Dong, Y and Li, X},
title = {Symbiotic conserved arbuscular mycorrhiza fungi supports plant health.},
journal = {The Science of the total environment},
volume = {955},
number = {},
pages = {176974},
doi = {10.1016/j.scitotenv.2024.176974},
pmid = {39419224},
issn = {1879-1026},
abstract = {Arbuscular mycorrhiza fungi (AMF) forms a multi-beneficial symbiotic relationship with the host plant, therefore it is considered to be an effective helper to promote plant health. However, failure to consider the source or universality of AMF is often unstable during application. Therefore, it is necessary to screen potential AMF inoculants based on the source and the relationship with host. In search of more effective and broad-spectrum AMF inoculants, we studied AMF community structure properties of healthy and diseased plants in 24 fields from four sampling sites. The results indicated that the environmental filtering effect of roots was obvious, which was manifested as a decrease of α-diversity from rhizosphere to root. Differences in α-diversity between healthy and diseased roots further indicate the importance of AMF communities within roots for maintaining plant health. Glomus is significantly enriched and dominant in healthy roots, independent of environment and phylogenically conserved. Spores were further isolated and evaluated for their disease-preventing and pro-growth properties. Based on whether they were symbiotic with plant and root-enrichment characteristics, isolated AMF spores were classified as symbiotic conserved, symbiotic non-conserved, and non-symbiotic AMF. After spores were propagated and inoculated to plant roots, only symbiotic conserved AMF significantly promoted plant growth and maintained health, highlighting the potential of symbiotic conserved AMF in sustainable plant production.},
}
RevDate: 2024-10-17
Symbiotic bacterial communities and carbon metabolic profiles of Acropora coral with varying health status under thermal stress.
Marine pollution bulletin, 209(Pt A):117116 pii:S0025-326X(24)01093-2 [Epub ahead of print].
Thermal-induced coral bleaching has received substantial research attention; however, the dynamics of symbiotic coral-associated bacterial communities are underexplored and the roles of coral with intermediate health status remain unclear. Using high-throughput sequencing and biochemical analyses, we found that the symbiotic zooxanthellae number gradually decreased with the increase of bleaching degree (non-bleached, semi-bleached, and fully-bleached) in the coral Acropora pruinosa. The semi-bleached host exhibited a relatively more complex microbial interaction network. For the carbon metabolic profiles, relatively higher carbon-fixing abilities observed in non-bleached coral symbiotic bacteria, followed by semi-bleached host, and lowest values appeared in fully-bleached coral. Partial least-squares pathway modeling revealed that bacterial community features and carbon metabolic function were directly related with health status, while temperature exerted a strong influence on the bleaching resilience. These findings can help us better understand the coral microecological feature and carbon metabolic potential under changing environment.
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@article {pmid39418876,
year = {2024},
author = {Qin, Y and Cheng, K and Jong, MC and Zheng, H and Cai, Z and Xiao, B and Zhou, J},
title = {Symbiotic bacterial communities and carbon metabolic profiles of Acropora coral with varying health status under thermal stress.},
journal = {Marine pollution bulletin},
volume = {209},
number = {Pt A},
pages = {117116},
doi = {10.1016/j.marpolbul.2024.117116},
pmid = {39418876},
issn = {1879-3363},
abstract = {Thermal-induced coral bleaching has received substantial research attention; however, the dynamics of symbiotic coral-associated bacterial communities are underexplored and the roles of coral with intermediate health status remain unclear. Using high-throughput sequencing and biochemical analyses, we found that the symbiotic zooxanthellae number gradually decreased with the increase of bleaching degree (non-bleached, semi-bleached, and fully-bleached) in the coral Acropora pruinosa. The semi-bleached host exhibited a relatively more complex microbial interaction network. For the carbon metabolic profiles, relatively higher carbon-fixing abilities observed in non-bleached coral symbiotic bacteria, followed by semi-bleached host, and lowest values appeared in fully-bleached coral. Partial least-squares pathway modeling revealed that bacterial community features and carbon metabolic function were directly related with health status, while temperature exerted a strong influence on the bleaching resilience. These findings can help us better understand the coral microecological feature and carbon metabolic potential under changing environment.},
}
RevDate: 2024-10-17
Functions of the Sinorhizobium meliloti LsrB Substrate-Binding Domain in Oxidized Glutathione Resistance, Alfalfa Nodulation Symbiosis, and Growth.
Journal of agricultural and food chemistry [Epub ahead of print].
To successfully colonize legume root nodules, rhizobia must effectively evade host-generated reactive oxygen species (ROS). LsrB, a redox regulator from Sinorhizobium meliloti, is essential for symbiosis with alfalfa (Medicago sativa). The three cysteine residues in LsrB's substrate domain play distinct roles in activating downstream redox genes. The study found that LsrB's substrate-binding domain, dependent on the cysteine residue Cys146, is involved in oxidized glutathione (GSSG) resistance and alfalfa nodulation symbiosis. LsrB homologues from other rhizobia, with Cys172/Cys238 or Cys146, enhance GSSG resistance and complement lsrB mutant's symbiotic nodulation. Substituting amino acids in Azorhizobium caulinodans LsrB with Cys restores lsrB mutant phenotypes. The lsrB deletion mutant shows increased sensitivity to NCR247, suggesting an interaction with host plant-derived NCRs in alfalfa nodules. Our findings reveal that the key cysteine residue in the LsrB's substrate domain is vital for rhizobium-legume symbiosis.
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@article {pmid39418129,
year = {2024},
author = {Zeng, S and Wang, S and Lin, Z and Jin, H and Li, H and Yu, H and Li, J and Yu, L and Luo, L},
title = {Functions of the Sinorhizobium meliloti LsrB Substrate-Binding Domain in Oxidized Glutathione Resistance, Alfalfa Nodulation Symbiosis, and Growth.},
journal = {Journal of agricultural and food chemistry},
volume = {},
number = {},
pages = {},
doi = {10.1021/acs.jafc.4c07925},
pmid = {39418129},
issn = {1520-5118},
abstract = {To successfully colonize legume root nodules, rhizobia must effectively evade host-generated reactive oxygen species (ROS). LsrB, a redox regulator from Sinorhizobium meliloti, is essential for symbiosis with alfalfa (Medicago sativa). The three cysteine residues in LsrB's substrate domain play distinct roles in activating downstream redox genes. The study found that LsrB's substrate-binding domain, dependent on the cysteine residue Cys146, is involved in oxidized glutathione (GSSG) resistance and alfalfa nodulation symbiosis. LsrB homologues from other rhizobia, with Cys172/Cys238 or Cys146, enhance GSSG resistance and complement lsrB mutant's symbiotic nodulation. Substituting amino acids in Azorhizobium caulinodans LsrB with Cys restores lsrB mutant phenotypes. The lsrB deletion mutant shows increased sensitivity to NCR247, suggesting an interaction with host plant-derived NCRs in alfalfa nodules. Our findings reveal that the key cysteine residue in the LsrB's substrate domain is vital for rhizobium-legume symbiosis.},
}
RevDate: 2024-10-19
CmpDate: 2024-10-17
Effects of the symbiotic bacteria, Caballeronia insecticola, on the life history parameters of Riptortus pedestris (Hemiptera: Alydidae) and their implications for the host population growth.
Journal of insect science (Online), 24(5):.
This study aimed to investigate the effects of symbiosis on the life history of host insects and address their implications at the host population level. We evaluated the effects of symbiotic bacteria Caballeronia insecticola on its host Riptortus pedestris (Fabricus) (Hemiptera: Alydidae) from cohorts for nymphal development, adult survivorship, and female reproduction. Then, life table parameters were compared between symbiotic and apo-symbiotic groups, and the effects of symbiosis on the abundance of R. pedestris were simulated for varying proportions of symbiotic individuals in host populations. We found that symbiosis significantly accelerated the nymphal development and reproductive maturation of females. However, symbiosis incurred survival cost on adult females, reducing their longevity by 28.6%. Nonetheless, symbiotic females laid significantly greater numbers of eggs than the apo-symbiotic during early adult ages. This early reproductive investment negated the adverse effect of their reduced longevity, resulting in the mean lifetime fecundity to not significantly differ between the 2 groups. Indeed, total cohort fecundity of the symbiotic group was 1.3-fold greater than that of the apo-symbiotic group. Life table analysis demonstrated shorter generation time and greater population growth rate in the symbiotic population. Finally, the simulation model results indicate that an increase in the proportion of symbiotic R. pedestris favored the population growth, increasing the population size by 1.9 times for every 25% increase in the proportion of symbiotic individuals. Our study demonstrates that symbiont-mediated changes in the life history parameters of host individuals favor the host population growth, despite substantial reduction in the female longevity.
Additional Links: PMID-39417594
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@article {pmid39417594,
year = {2024},
author = {Kho, JW and Jung, M and Lee, DH},
title = {Effects of the symbiotic bacteria, Caballeronia insecticola, on the life history parameters of Riptortus pedestris (Hemiptera: Alydidae) and their implications for the host population growth.},
journal = {Journal of insect science (Online)},
volume = {24},
number = {5},
pages = {},
pmid = {39417594},
issn = {1536-2442},
support = {2021R1A2C1010679//National Research Foundation of Korea/ ; //Basic Science Research Program/ ; RS-2023-00246911//Ministry of Education/ ; },
mesh = {Animals ; *Symbiosis ; Female ; *Nymph/growth & development/physiology/microbiology ; Life History Traits ; Population Growth ; Longevity ; Hemiptera/growth & development/microbiology/physiology ; Heteroptera/growth & development/physiology/microbiology ; Reproduction ; Male ; Fertility ; },
abstract = {This study aimed to investigate the effects of symbiosis on the life history of host insects and address their implications at the host population level. We evaluated the effects of symbiotic bacteria Caballeronia insecticola on its host Riptortus pedestris (Fabricus) (Hemiptera: Alydidae) from cohorts for nymphal development, adult survivorship, and female reproduction. Then, life table parameters were compared between symbiotic and apo-symbiotic groups, and the effects of symbiosis on the abundance of R. pedestris were simulated for varying proportions of symbiotic individuals in host populations. We found that symbiosis significantly accelerated the nymphal development and reproductive maturation of females. However, symbiosis incurred survival cost on adult females, reducing their longevity by 28.6%. Nonetheless, symbiotic females laid significantly greater numbers of eggs than the apo-symbiotic during early adult ages. This early reproductive investment negated the adverse effect of their reduced longevity, resulting in the mean lifetime fecundity to not significantly differ between the 2 groups. Indeed, total cohort fecundity of the symbiotic group was 1.3-fold greater than that of the apo-symbiotic group. Life table analysis demonstrated shorter generation time and greater population growth rate in the symbiotic population. Finally, the simulation model results indicate that an increase in the proportion of symbiotic R. pedestris favored the population growth, increasing the population size by 1.9 times for every 25% increase in the proportion of symbiotic individuals. Our study demonstrates that symbiont-mediated changes in the life history parameters of host individuals favor the host population growth, despite substantial reduction in the female longevity.},
}
MeSH Terms:
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Animals
*Symbiosis
Female
*Nymph/growth & development/physiology/microbiology
Life History Traits
Population Growth
Longevity
Hemiptera/growth & development/microbiology/physiology
Heteroptera/growth & development/physiology/microbiology
Reproduction
Male
Fertility
RevDate: 2024-10-19
The composition of the arbuscular mycorrhizal fungal bacteriome is species dependent.
Environmental microbiome, 19(1):77.
BACKGROUND: In addition to their role as endosymbionts for plant roots, arbuscular mycorrhizal fungi (AMF) engage in complex interactions with various soil microorganisms, the rhizosphere, and the root endosphere of host plants. They also host diverse prokaryotic groups within their mycelia, contributing to what is termed multipartite symbiosis. In this study, we examined the impact of three AMF species-Rhizophagus irregularis, R. clarus, and R. cerebriforme-combined with microbial bioaugmentation on the diversity and composition of bacterial communities in the mycelia and hyphosphere. Using a microcosm design to separate the influence of host plant roots from AMF mycelia and Illumina MiSeq amplicon sequencing to analyze the bacterial communities.
RESULTS: Our results revealed that, while AMF identity and microbial bioaugmentation did not affect the structure of bacterial communities in the hyphosphere soil, they significantly altered the communities associated with their mycelia. Although all three AMF species belong to the same genus, with R. irregularis and R. clarus being closely related compared to R. cerebriforme, we observed variations in the bacterial communities associated with their mycelia. Interestingly, the mycelial bacterial community of R. cerebriforme contained 60 bacteriome core taxa exclusive to it, while R. clarus and R. irregularis had 25 and 9 exclusive taxa, respectively.
CONCLUSION: This study suggests that organismal phylogeny influences the bacterial communities associated with AMF mycelia. These findings provide new insights into AMF and bacterial interactions, which are crucial for the successful deployment of AMF inoculants. The taxonomic diversity of AMF inoculants is important for engineering the plant microbiome and enhancing ecosystem services.
Additional Links: PMID-39415218
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@article {pmid39415218,
year = {2024},
author = {Lahrach, Z and Legeay, J and Ahmed, B and Hijri, M},
title = {The composition of the arbuscular mycorrhizal fungal bacteriome is species dependent.},
journal = {Environmental microbiome},
volume = {19},
number = {1},
pages = {77},
pmid = {39415218},
issn = {2524-6372},
abstract = {BACKGROUND: In addition to their role as endosymbionts for plant roots, arbuscular mycorrhizal fungi (AMF) engage in complex interactions with various soil microorganisms, the rhizosphere, and the root endosphere of host plants. They also host diverse prokaryotic groups within their mycelia, contributing to what is termed multipartite symbiosis. In this study, we examined the impact of three AMF species-Rhizophagus irregularis, R. clarus, and R. cerebriforme-combined with microbial bioaugmentation on the diversity and composition of bacterial communities in the mycelia and hyphosphere. Using a microcosm design to separate the influence of host plant roots from AMF mycelia and Illumina MiSeq amplicon sequencing to analyze the bacterial communities.
RESULTS: Our results revealed that, while AMF identity and microbial bioaugmentation did not affect the structure of bacterial communities in the hyphosphere soil, they significantly altered the communities associated with their mycelia. Although all three AMF species belong to the same genus, with R. irregularis and R. clarus being closely related compared to R. cerebriforme, we observed variations in the bacterial communities associated with their mycelia. Interestingly, the mycelial bacterial community of R. cerebriforme contained 60 bacteriome core taxa exclusive to it, while R. clarus and R. irregularis had 25 and 9 exclusive taxa, respectively.
CONCLUSION: This study suggests that organismal phylogeny influences the bacterial communities associated with AMF mycelia. These findings provide new insights into AMF and bacterial interactions, which are crucial for the successful deployment of AMF inoculants. The taxonomic diversity of AMF inoculants is important for engineering the plant microbiome and enhancing ecosystem services.},
}
RevDate: 2024-10-16
The role of the AHR in host-pathogen interactions.
Nature reviews. Immunology [Epub ahead of print].
Host-microorganism encounters take place in many different ways and with different types of outcomes. Three major types of microorganisms need to be distinguished: (1) pathogens that cause harm to the host and must be controlled; (2) environmental microorganisms that can be ignored but must be controlled at higher abundance; and (3) symbiotic microbiota that require support by the host. Recent evidence indicates that the aryl hydrocarbon receptor (AHR) senses and initiates signalling and gene expression in response to a plethora of microorganisms and infectious conditions. It was originally identified as a receptor that binds xenobiotics. However, it was subsequently found to have a critical role in numerous biological processes, including immunity and inflammation and was recently classified as a pattern recognition receptor. Here we review the role of the AHR in host-pathogen interactions, focusing on AHR sensing of different microbial classes, the ligands involved, responses elicited and disease outcomes. Moreover, we explore the therapeutic potential of targeting the AHR in the context of infection.
Additional Links: PMID-39415055
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@article {pmid39415055,
year = {2024},
author = {Barreira-Silva, P and Lian, Y and Kaufmann, SHE and Moura-Alves, P},
title = {The role of the AHR in host-pathogen interactions.},
journal = {Nature reviews. Immunology},
volume = {},
number = {},
pages = {},
pmid = {39415055},
issn = {1474-1741},
abstract = {Host-microorganism encounters take place in many different ways and with different types of outcomes. Three major types of microorganisms need to be distinguished: (1) pathogens that cause harm to the host and must be controlled; (2) environmental microorganisms that can be ignored but must be controlled at higher abundance; and (3) symbiotic microbiota that require support by the host. Recent evidence indicates that the aryl hydrocarbon receptor (AHR) senses and initiates signalling and gene expression in response to a plethora of microorganisms and infectious conditions. It was originally identified as a receptor that binds xenobiotics. However, it was subsequently found to have a critical role in numerous biological processes, including immunity and inflammation and was recently classified as a pattern recognition receptor. Here we review the role of the AHR in host-pathogen interactions, focusing on AHR sensing of different microbial classes, the ligands involved, responses elicited and disease outcomes. Moreover, we explore the therapeutic potential of targeting the AHR in the context of infection.},
}
RevDate: 2024-10-18
CmpDate: 2024-10-16
Inorganic nitrogen inhibits symbiotic nitrogen fixation through blocking NRAMP2-mediated iron delivery in soybean nodules.
Nature communications, 15(1):8946.
Symbiotic nitrogen fixation (SNF) in legume-rhizobia serves as a sustainable source of nitrogen (N) in agriculture. However, the addition of inorganic N fertilizers significantly inhibits SNF, and the underlying mechanisms remain not-well understood. Here, we report that inorganic N disrupts iron (Fe) homeostasis in soybean nodules, leading to a decrease in SNF efficiency. This disruption is attributed to the inhibition of the Fe transporter genes Natural Resistance-Associated Macrophage Protein 2a and 2b (GmNRAMP2a&2b) by inorganic N. GmNRAMP2a&2b are predominantly localized at the tonoplast of uninfected nodule tissues, affecting Fe transfer to infected cells and consequently, modulating SNF efficiency. In addition, we identified a pair of N-signal regulators, nitrogen-regulated GARP-type transcription factors 1a and 1b (GmNIGT1a&1b), that negatively regulate the expression of GmNRAMP2a&2b, which establishes a link between N signaling and Fe homeostasis in nodules. Our findings reveal a plausible mechanism by which soybean adjusts SNF efficiency through Fe allocation in response to fluctuating inorganic N conditions, offering valuable insights for optimizing N and Fe management in legume-based agricultural systems.
Additional Links: PMID-39414817
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@article {pmid39414817,
year = {2024},
author = {Zhou, M and Li, Y and Yao, XL and Zhang, J and Liu, S and Cao, HR and Bai, S and Chen, CQ and Zhang, DX and Xu, A and Lei, JN and Mao, QZ and Zhou, Y and Duanmu, DQ and Guan, YF and Chen, ZC},
title = {Inorganic nitrogen inhibits symbiotic nitrogen fixation through blocking NRAMP2-mediated iron delivery in soybean nodules.},
journal = {Nature communications},
volume = {15},
number = {1},
pages = {8946},
pmid = {39414817},
issn = {2041-1723},
mesh = {*Glycine max/metabolism/genetics/microbiology ; *Nitrogen Fixation/drug effects ; *Iron/metabolism ; *Root Nodules, Plant/metabolism ; *Nitrogen/metabolism ; *Symbiosis ; *Gene Expression Regulation, Plant/drug effects ; *Plant Proteins/metabolism/genetics ; *Cation Transport Proteins/metabolism/genetics ; Homeostasis ; Bradyrhizobium/metabolism/genetics ; },
abstract = {Symbiotic nitrogen fixation (SNF) in legume-rhizobia serves as a sustainable source of nitrogen (N) in agriculture. However, the addition of inorganic N fertilizers significantly inhibits SNF, and the underlying mechanisms remain not-well understood. Here, we report that inorganic N disrupts iron (Fe) homeostasis in soybean nodules, leading to a decrease in SNF efficiency. This disruption is attributed to the inhibition of the Fe transporter genes Natural Resistance-Associated Macrophage Protein 2a and 2b (GmNRAMP2a&2b) by inorganic N. GmNRAMP2a&2b are predominantly localized at the tonoplast of uninfected nodule tissues, affecting Fe transfer to infected cells and consequently, modulating SNF efficiency. In addition, we identified a pair of N-signal regulators, nitrogen-regulated GARP-type transcription factors 1a and 1b (GmNIGT1a&1b), that negatively regulate the expression of GmNRAMP2a&2b, which establishes a link between N signaling and Fe homeostasis in nodules. Our findings reveal a plausible mechanism by which soybean adjusts SNF efficiency through Fe allocation in response to fluctuating inorganic N conditions, offering valuable insights for optimizing N and Fe management in legume-based agricultural systems.},
}
MeSH Terms:
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hide MeSH Terms
*Glycine max/metabolism/genetics/microbiology
*Nitrogen Fixation/drug effects
*Iron/metabolism
*Root Nodules, Plant/metabolism
*Nitrogen/metabolism
*Symbiosis
*Gene Expression Regulation, Plant/drug effects
*Plant Proteins/metabolism/genetics
*Cation Transport Proteins/metabolism/genetics
Homeostasis
Bradyrhizobium/metabolism/genetics
RevDate: 2024-10-16
Unique ecology of biofilms and flocs: Bacterial composition, assembly, interaction, and nitrogen metabolism within deteriorated bioreactor inoculated with mature partial nitrification-anammox sludge.
Bioresource technology pii:S0960-8524(24)01347-6 [Epub ahead of print].
This work unraveled discrepant ecological patterns between biofilms and flocs in a deteriorated bioreactor inoculated with mature partial nitrification-anammox (PN/A) sludge. Based on 16S rRNA analysis, a comprehensive evaluation of neutral and null models, along with niche width, delineated that the bacterial community assembly in biofilms and flocs was dominantly driven by the stochastic process, and dispersal limitation critically shaped the community assembly. Co-occurrence network analysis revealed that environmental stress caused decentralized and fragmented bacterial colonies, and anammox bacteria were mainly peripheral in biofilms network and less involved in interspecific interactions. Simultaneous PN/A and partial denitrification-anammox (PD/A) processes were identified, whereas PN and PD process primarily occurred in the biofilms and flocs, respectively, as evidenced by metagenomics. Collectively, these outcomes are expected to deepen the basic understanding of complex microbial community and nitrogen metabolism under environmental disturbance, thereby better characterizing and serving the artificial ecosystems.
Additional Links: PMID-39414169
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@article {pmid39414169,
year = {2024},
author = {Zhang, B and Zhang, N and Sui, H and Xue, R and Qiao, S},
title = {Unique ecology of biofilms and flocs: Bacterial composition, assembly, interaction, and nitrogen metabolism within deteriorated bioreactor inoculated with mature partial nitrification-anammox sludge.},
journal = {Bioresource technology},
volume = {},
number = {},
pages = {131643},
doi = {10.1016/j.biortech.2024.131643},
pmid = {39414169},
issn = {1873-2976},
abstract = {This work unraveled discrepant ecological patterns between biofilms and flocs in a deteriorated bioreactor inoculated with mature partial nitrification-anammox (PN/A) sludge. Based on 16S rRNA analysis, a comprehensive evaluation of neutral and null models, along with niche width, delineated that the bacterial community assembly in biofilms and flocs was dominantly driven by the stochastic process, and dispersal limitation critically shaped the community assembly. Co-occurrence network analysis revealed that environmental stress caused decentralized and fragmented bacterial colonies, and anammox bacteria were mainly peripheral in biofilms network and less involved in interspecific interactions. Simultaneous PN/A and partial denitrification-anammox (PD/A) processes were identified, whereas PN and PD process primarily occurred in the biofilms and flocs, respectively, as evidenced by metagenomics. Collectively, these outcomes are expected to deepen the basic understanding of complex microbial community and nitrogen metabolism under environmental disturbance, thereby better characterizing and serving the artificial ecosystems.},
}
RevDate: 2024-10-18
Host genotype and age shape the microbial community in the rhizosphere soils of Camellia forests.
Frontiers in microbiology, 15:1440255.
Microbiota living in the rhizosphere influences plant growth and fitness, from the opposite perspective; whether host genotypes control its root microbiota is of great interest to forest breeders and microbiologists. To improve low-yield plantations and promote sustainable management of Camellia oleifera, high-throughput sequencing was used to study the chemical properties and microbiome in rhizosphere soil of Camellia forests under three genotypes (common C. oleifera, local C. gauchowensis, and C. chekiangoleosa) and three growth stages (sapling stage at 4-year-old, primary fruit stage at 7-year-old, and full fruiting stage at 11-year-old). The results showed that the rhizosphere soil organic matter (OM), nutrient concentrations, diversity, and community composition of the microbiome were significantly varied among different Camellia genotypes. The relative abundance of symbiotic and pathotrophic fungi in the rhizosphere soil of C. chekiangoleosa was significantly higher than that of C. gauchowensis. Concentrations of OM, available phosphorus (AP), and bacterial alpha diversity increased with tree age. Fungi of Saitozyma, Mortierella, and Glomeromycota and bacteria of Burkholderia-Caballeronia-Paraburkholderia and Vicinamibacterales had potential for fertilizer development for Camellia plantation. Camellia genotypes and growth stages were significantly correlated with the rhizosphere soil pH, OM, and available potassium (AK). Soil pH and OM were key factors that affected the microbiome in the Camellia rhizosphere soils. In conclusion, tree genotypes and growth stages shaped microbial communities in Camellia rhizosphere soils, and some plant growth-promoting rhizobacteria were identified as preliminary candidates for improving Camellia plantation growth.
Additional Links: PMID-39411438
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@article {pmid39411438,
year = {2024},
author = {Lv, J and Huo, C and Zhang, J and Huang, Y and Su, Y and Lv, Y and Xie, X and Chen, Z},
title = {Host genotype and age shape the microbial community in the rhizosphere soils of Camellia forests.},
journal = {Frontiers in microbiology},
volume = {15},
number = {},
pages = {1440255},
pmid = {39411438},
issn = {1664-302X},
abstract = {Microbiota living in the rhizosphere influences plant growth and fitness, from the opposite perspective; whether host genotypes control its root microbiota is of great interest to forest breeders and microbiologists. To improve low-yield plantations and promote sustainable management of Camellia oleifera, high-throughput sequencing was used to study the chemical properties and microbiome in rhizosphere soil of Camellia forests under three genotypes (common C. oleifera, local C. gauchowensis, and C. chekiangoleosa) and three growth stages (sapling stage at 4-year-old, primary fruit stage at 7-year-old, and full fruiting stage at 11-year-old). The results showed that the rhizosphere soil organic matter (OM), nutrient concentrations, diversity, and community composition of the microbiome were significantly varied among different Camellia genotypes. The relative abundance of symbiotic and pathotrophic fungi in the rhizosphere soil of C. chekiangoleosa was significantly higher than that of C. gauchowensis. Concentrations of OM, available phosphorus (AP), and bacterial alpha diversity increased with tree age. Fungi of Saitozyma, Mortierella, and Glomeromycota and bacteria of Burkholderia-Caballeronia-Paraburkholderia and Vicinamibacterales had potential for fertilizer development for Camellia plantation. Camellia genotypes and growth stages were significantly correlated with the rhizosphere soil pH, OM, and available potassium (AK). Soil pH and OM were key factors that affected the microbiome in the Camellia rhizosphere soils. In conclusion, tree genotypes and growth stages shaped microbial communities in Camellia rhizosphere soils, and some plant growth-promoting rhizobacteria were identified as preliminary candidates for improving Camellia plantation growth.},
}
RevDate: 2024-10-18
From the inside out: Were the cuticular Pseudonocardia bacteria of fungus-farming ants originally domesticated as gut symbionts?.
PNAS nexus, 3(10):pgae391.
The mutualistic interaction specificity between attine ants and antibiotic-producing Actinobacteria has been controversial because Pseudonocardia strains cannot always be isolated from worker cuticles across attine ant species, while other actinobacteria can apparently replace Pseudonocardia and also inhibit growth of Escovopsis mycopathogens. Here we report that across field samples of Panamanian species: (i) Cuticular Pseudonocardia were largely restricted to species in the crown of the attine phylogeny and their appearance likely coincided with the first attines colonizing Central/North America. (ii) The phylogenetically basal attines almost always had cuticular associations with other Actinobacteria than Pseudonocardia. (iii) The sub-cuticular glands nourishing cuticular bacteria appear to be homologous throughout the phylogeny, consistent with an ancient general attine-Actinobacteria association. (iv) The basal attine species investigated always had Pseudonocardia as gut symbionts while Pseudonocardia presence appeared mutually exclusive between cuticular and gut microbiomes. (v) Gut-associated Pseudonocardia were phylogenetically ancestral while cuticular symbionts formed a derived crown group within the Pseudonocardia phylogeny. We further show that laboratory colonies often secondarily acquire cuticular Actinobacteria that they do not associate with in the field, suggesting that many previous studies were uninformative for questions of co-adaptation in the wild. An exhaustive literature survey showed that published studies concur with our present results, provided that they analyzed field colonies and that Actinobacteria were specifically isolated from worker cuticles shortly after field collection. Our results offer several testable hypotheses for a better overall understanding of attine-Pseudonocardia interaction dynamics and putative coevolution throughout the Americas.
Additional Links: PMID-39411080
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@article {pmid39411080,
year = {2024},
author = {Innocent, TM and Sapountzis, P and Zhukova, M and Poulsen, M and Schiøtt, M and Nash, DR and Boomsma, JJ},
title = {From the inside out: Were the cuticular Pseudonocardia bacteria of fungus-farming ants originally domesticated as gut symbionts?.},
journal = {PNAS nexus},
volume = {3},
number = {10},
pages = {pgae391},
pmid = {39411080},
issn = {2752-6542},
abstract = {The mutualistic interaction specificity between attine ants and antibiotic-producing Actinobacteria has been controversial because Pseudonocardia strains cannot always be isolated from worker cuticles across attine ant species, while other actinobacteria can apparently replace Pseudonocardia and also inhibit growth of Escovopsis mycopathogens. Here we report that across field samples of Panamanian species: (i) Cuticular Pseudonocardia were largely restricted to species in the crown of the attine phylogeny and their appearance likely coincided with the first attines colonizing Central/North America. (ii) The phylogenetically basal attines almost always had cuticular associations with other Actinobacteria than Pseudonocardia. (iii) The sub-cuticular glands nourishing cuticular bacteria appear to be homologous throughout the phylogeny, consistent with an ancient general attine-Actinobacteria association. (iv) The basal attine species investigated always had Pseudonocardia as gut symbionts while Pseudonocardia presence appeared mutually exclusive between cuticular and gut microbiomes. (v) Gut-associated Pseudonocardia were phylogenetically ancestral while cuticular symbionts formed a derived crown group within the Pseudonocardia phylogeny. We further show that laboratory colonies often secondarily acquire cuticular Actinobacteria that they do not associate with in the field, suggesting that many previous studies were uninformative for questions of co-adaptation in the wild. An exhaustive literature survey showed that published studies concur with our present results, provided that they analyzed field colonies and that Actinobacteria were specifically isolated from worker cuticles shortly after field collection. Our results offer several testable hypotheses for a better overall understanding of attine-Pseudonocardia interaction dynamics and putative coevolution throughout the Americas.},
}
RevDate: 2024-10-16
Effect of aeration pretreatment on anaerobic digestion of swine manure.
Environmental technology [Epub ahead of print].
To investigate the effects of aeration pretreatment on the anaerobic digestion (AD) of swine manure, five pretreatment groups were established with dissolved oxygen (DO) in each group set to 0.0, 0.4, 0.8, 1.4, and 2.0 mg/L, respectively. The results demonstrated that compared to the non-aeration group, methane production increased to varying degrees with different aeration pretreatments (AP), with a maximum increase of 27.98% (DO = 2.0 mg/L). AP reduced the hydrogen sulfide (H2S) content of biogas. The H2S concentration in the DO = 2.0 mg/L was only 0.209%, and this represented an increased H2S removal rate of 49.27% compared to that of the DO = 0.0 mg/L (0.412%). Simultaneously, AP increases the hydrolysis rate. When the DO concentration reached 2.0 mg/L, the hydrolysis rate reached its maximum. An increase in the hydrolysis rate further enhanced the removal rate of organic matter. The organic matter removal rate was highest (36.96%) at DO = 2.0 mg/L. AP effectively prolonged the methane generation time and shortened the lag time of methane generation. AP creates a brief micro aerobic environment, accelerates substrate hydrolysis, and promotes the production and consumption of total volatile fatty acids, particularly acetic acid. Additionally, AP promoted the symbiotic relationship between Caldicoprobacter (20.93%-34.96%) and Metanosaeta (14.73%-18.45%).
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@article {pmid39410839,
year = {2024},
author = {Wang, J and Wang, X and Bian, C and Liu, J and Xiao, B},
title = {Effect of aeration pretreatment on anaerobic digestion of swine manure.},
journal = {Environmental technology},
volume = {},
number = {},
pages = {1-17},
doi = {10.1080/09593330.2024.2416094},
pmid = {39410839},
issn = {1479-487X},
abstract = {To investigate the effects of aeration pretreatment on the anaerobic digestion (AD) of swine manure, five pretreatment groups were established with dissolved oxygen (DO) in each group set to 0.0, 0.4, 0.8, 1.4, and 2.0 mg/L, respectively. The results demonstrated that compared to the non-aeration group, methane production increased to varying degrees with different aeration pretreatments (AP), with a maximum increase of 27.98% (DO = 2.0 mg/L). AP reduced the hydrogen sulfide (H2S) content of biogas. The H2S concentration in the DO = 2.0 mg/L was only 0.209%, and this represented an increased H2S removal rate of 49.27% compared to that of the DO = 0.0 mg/L (0.412%). Simultaneously, AP increases the hydrolysis rate. When the DO concentration reached 2.0 mg/L, the hydrolysis rate reached its maximum. An increase in the hydrolysis rate further enhanced the removal rate of organic matter. The organic matter removal rate was highest (36.96%) at DO = 2.0 mg/L. AP effectively prolonged the methane generation time and shortened the lag time of methane generation. AP creates a brief micro aerobic environment, accelerates substrate hydrolysis, and promotes the production and consumption of total volatile fatty acids, particularly acetic acid. Additionally, AP promoted the symbiotic relationship between Caldicoprobacter (20.93%-34.96%) and Metanosaeta (14.73%-18.45%).},
}
RevDate: 2024-10-16
Azolla as a Safe Food: Suppression of Cyanotoxin-Related Genes and Cyanotoxin Production in Its Symbiont, Nostoc azollae.
Plants (Basel, Switzerland), 13(19): pii:plants13192707.
The floating freshwater fern Azolla is the only plant that retains an endocyanobiont, Nostoc azollae (aka Anabaena azollae), during its sexual and asexual reproduction. The increased interest in Azolla as a potential source of food and its unique evolutionary history have raised questions about its cyanotoxin content and genome. Cyanotoxins are potent toxins synthesized by cyanobacteria which have an anti-herbivore effect but have also been linked to neurodegenerative disorders including Alzheimer's and Parkinson's diseases, liver and kidney failure, muscle paralysis, and other severe health issues. In this study, we investigated 48 accessions of Azolla-Nostoc symbiosis for the presence of genes coding microcystin, nodularin, cylindrospermopsin and saxitoxin, and BLAST analysis for anatoxin-a. We also investigated the presence of the neurotoxin β-N-methylamino-L-alanine (BMAA) in Azolla and N. azollae through LC-MS/MS. The PCR amplification of saxitoxin, cylindrospermospin, microcystin, and nodularin genes showed that Azolla and its cyanobiont N. azollae do not have the genes to synthesize these cyanotoxins. Additionally, the matching of the anatoxin-a gene to the sequenced N. azollae genome does not indicate the presence of the anatoxin-a gene. The LC-MS/MS analysis showed that BMAA and its isomers AEG and DAB are absent from Azolla and Nostoc azollae. Azolla therefore has the potential to safely feed millions of people due to its rapid growth while free-floating on shallow fresh water without the need for nitrogen fertilizers.
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@article {pmid39409577,
year = {2024},
author = {Bujak, JP and Pereira, AL and Azevedo, J and Bujak, AA and Leshyk, V and Pham Gia, M and Stadtlander, T and Vasconcelos, V and Winstead, DJ},
title = {Azolla as a Safe Food: Suppression of Cyanotoxin-Related Genes and Cyanotoxin Production in Its Symbiont, Nostoc azollae.},
journal = {Plants (Basel, Switzerland)},
volume = {13},
number = {19},
pages = {},
doi = {10.3390/plants13192707},
pmid = {39409577},
issn = {2223-7747},
support = {Research on Emergency Food Resilience project//Contributions made by D. Winstead were supported financially by Open Philanthropy for the Penn State University/ ; },
abstract = {The floating freshwater fern Azolla is the only plant that retains an endocyanobiont, Nostoc azollae (aka Anabaena azollae), during its sexual and asexual reproduction. The increased interest in Azolla as a potential source of food and its unique evolutionary history have raised questions about its cyanotoxin content and genome. Cyanotoxins are potent toxins synthesized by cyanobacteria which have an anti-herbivore effect but have also been linked to neurodegenerative disorders including Alzheimer's and Parkinson's diseases, liver and kidney failure, muscle paralysis, and other severe health issues. In this study, we investigated 48 accessions of Azolla-Nostoc symbiosis for the presence of genes coding microcystin, nodularin, cylindrospermopsin and saxitoxin, and BLAST analysis for anatoxin-a. We also investigated the presence of the neurotoxin β-N-methylamino-L-alanine (BMAA) in Azolla and N. azollae through LC-MS/MS. The PCR amplification of saxitoxin, cylindrospermospin, microcystin, and nodularin genes showed that Azolla and its cyanobiont N. azollae do not have the genes to synthesize these cyanotoxins. Additionally, the matching of the anatoxin-a gene to the sequenced N. azollae genome does not indicate the presence of the anatoxin-a gene. The LC-MS/MS analysis showed that BMAA and its isomers AEG and DAB are absent from Azolla and Nostoc azollae. Azolla therefore has the potential to safely feed millions of people due to its rapid growth while free-floating on shallow fresh water without the need for nitrogen fertilizers.},
}
RevDate: 2024-10-16
CmpDate: 2024-10-16
Bacteria and Allergic Diseases.
International journal of molecular sciences, 25(19): pii:ijms251910298.
Microorganisms colonize all barrier tissues and are present on the skin and all mucous membranes from birth. Bacteria have many ways of influencing the host organism, including activation of innate immunity receptors by pathogen-associated molecular patterns and synthesis of various chemical compounds, such as vitamins, short-chain fatty acids, bacteriocins, toxins. Bacteria, using extracellular vesicles, can also introduce high-molecular compounds, such as proteins and nucleic acids, into the cell, regulating the metabolic pathways of the host cells. Epithelial cells and immune cells recognize bacterial bioregulators and, depending on the microenvironment and context, determine the direction and intensity of the immune response. A large number of factors influence the maintenance of symbiotic microflora, the diversity of which protects hosts against pathogen colonization. Reduced bacterial diversity is associated with pathogen dominance and allergic diseases of the skin, gastrointestinal tract, and upper and lower respiratory tract, as seen in atopic dermatitis, allergic rhinitis, chronic rhinosinusitis, food allergies, and asthma. Understanding the multifactorial influence of microflora on maintaining health and disease determines the effectiveness of therapy and disease prevention and changes our food preferences and lifestyle to maintain health and active longevity.
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@article {pmid39408628,
year = {2024},
author = {Guryanova, SV},
title = {Bacteria and Allergic Diseases.},
journal = {International journal of molecular sciences},
volume = {25},
number = {19},
pages = {},
doi = {10.3390/ijms251910298},
pmid = {39408628},
issn = {1422-0067},
mesh = {Humans ; *Hypersensitivity/microbiology/immunology ; *Bacteria/metabolism ; Animals ; Microbiota ; },
abstract = {Microorganisms colonize all barrier tissues and are present on the skin and all mucous membranes from birth. Bacteria have many ways of influencing the host organism, including activation of innate immunity receptors by pathogen-associated molecular patterns and synthesis of various chemical compounds, such as vitamins, short-chain fatty acids, bacteriocins, toxins. Bacteria, using extracellular vesicles, can also introduce high-molecular compounds, such as proteins and nucleic acids, into the cell, regulating the metabolic pathways of the host cells. Epithelial cells and immune cells recognize bacterial bioregulators and, depending on the microenvironment and context, determine the direction and intensity of the immune response. A large number of factors influence the maintenance of symbiotic microflora, the diversity of which protects hosts against pathogen colonization. Reduced bacterial diversity is associated with pathogen dominance and allergic diseases of the skin, gastrointestinal tract, and upper and lower respiratory tract, as seen in atopic dermatitis, allergic rhinitis, chronic rhinosinusitis, food allergies, and asthma. Understanding the multifactorial influence of microflora on maintaining health and disease determines the effectiveness of therapy and disease prevention and changes our food preferences and lifestyle to maintain health and active longevity.},
}
MeSH Terms:
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Humans
*Hypersensitivity/microbiology/immunology
*Bacteria/metabolism
Animals
Microbiota
RevDate: 2024-10-16
CmpDate: 2024-10-16
Secondary Metabolites from Australian Lichens Ramalina celastri and Stereocaulon ramulosum Affect Growth and Metabolism of Photobiont Asterochloris erici through Allelopathy.
Molecules (Basel, Switzerland), 29(19): pii:molecules29194620.
In the present work, the phytotoxic effects of secondary metabolites extracted from lichen Ramalina celastri (usnic acid) and lichen Stereocaulon ramulosum (a naturally occurring mixture of atranorin and perlatolic acid, approx. 3:1) on cultures of the aposymbiotically grown lichen photobiont Asterochloris erici were evaluated. Algae were cultivated on the surface of glass microfiber disks with applied crystals of lichen extracts for 14 days. The toxicity of each extract was tested at the two selected doses in quantities of 0.01 mg/disk and 0.1 mg/disk. Cytotoxicity of lichen extracts was assessed using selected physiological parameters, such as growth (biomass production) of photobiont cultures, content of soluble proteins, chlorophyll a fluorescence, chlorophyll a integrity, contents of chlorophylls and total carotenoids, hydrogen peroxide, superoxide anion, TBARS, ascorbic acid (AsA), reduced (GSH) and oxidized (GSSG) glutathione, and composition of selected organic acids of the Krebs cycle. The application of both tested metabolic extracts decreased the growth of photobiont cells in a dose-dependent manner; however, a mixture of atranorin and perlatolic acid was more effective when compared to usnic acid at the same dose tested. A higher degree of cytotoxicity of extracts from lichen S. ramulosum when compared to identical doses of extracts from lichen R. celastri was also confirmed by a more pronounced decrease in chlorophyll a fluorescence and chlorophyll a integrity, decreased content of chlorophylls and total carotenoids, increased production of hydrogen peroxide and superoxide anion, peroxidation of membrane lipids (assessed as TBARS), and a strong decrease in non-enzymatic antioxidants such as AsA, GSH, and GSSG. The cytotoxicity of lichen compounds was confirmed by a strong alteration in the composition of selected organic acids included in the Krebs cycle. The increased ratio between pyruvic acid and citric acid was a very sensitive parameter of phytotoxicity of lichen secondary metabolites to the algal partner of symbiosis. Secondary metabolites of lichens are potent allelochemicals and play significant roles in maintaining the balance between mycobionts and photobionts, forming lichen thallus.
Additional Links: PMID-39407550
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@article {pmid39407550,
year = {2024},
author = {Bačkor, M and Kecsey, D and Drábová, B and Urminská, D and Šemeláková, M and Goga, M},
title = {Secondary Metabolites from Australian Lichens Ramalina celastri and Stereocaulon ramulosum Affect Growth and Metabolism of Photobiont Asterochloris erici through Allelopathy.},
journal = {Molecules (Basel, Switzerland)},
volume = {29},
number = {19},
pages = {},
doi = {10.3390/molecules29194620},
pmid = {39407550},
issn = {1420-3049},
support = {APVV-21-0289//Slovak Research and Development Agency/ ; 008SPU-4/2023, 009UPJS-4/2023//Slovak Grant Agency KEGA/ ; VEGA 1/0252/24//Slovak Grant Agency VEGA/ ; },
mesh = {*Lichens/metabolism/chemistry/growth & development ; *Secondary Metabolism ; *Allelopathy ; Benzofurans/pharmacology/metabolism/chemistry ; Chlorophyll A/metabolism ; Chlorophyll/metabolism ; Ascomycota/metabolism/drug effects ; Carotenoids/metabolism ; Hydroxybenzoates ; },
abstract = {In the present work, the phytotoxic effects of secondary metabolites extracted from lichen Ramalina celastri (usnic acid) and lichen Stereocaulon ramulosum (a naturally occurring mixture of atranorin and perlatolic acid, approx. 3:1) on cultures of the aposymbiotically grown lichen photobiont Asterochloris erici were evaluated. Algae were cultivated on the surface of glass microfiber disks with applied crystals of lichen extracts for 14 days. The toxicity of each extract was tested at the two selected doses in quantities of 0.01 mg/disk and 0.1 mg/disk. Cytotoxicity of lichen extracts was assessed using selected physiological parameters, such as growth (biomass production) of photobiont cultures, content of soluble proteins, chlorophyll a fluorescence, chlorophyll a integrity, contents of chlorophylls and total carotenoids, hydrogen peroxide, superoxide anion, TBARS, ascorbic acid (AsA), reduced (GSH) and oxidized (GSSG) glutathione, and composition of selected organic acids of the Krebs cycle. The application of both tested metabolic extracts decreased the growth of photobiont cells in a dose-dependent manner; however, a mixture of atranorin and perlatolic acid was more effective when compared to usnic acid at the same dose tested. A higher degree of cytotoxicity of extracts from lichen S. ramulosum when compared to identical doses of extracts from lichen R. celastri was also confirmed by a more pronounced decrease in chlorophyll a fluorescence and chlorophyll a integrity, decreased content of chlorophylls and total carotenoids, increased production of hydrogen peroxide and superoxide anion, peroxidation of membrane lipids (assessed as TBARS), and a strong decrease in non-enzymatic antioxidants such as AsA, GSH, and GSSG. The cytotoxicity of lichen compounds was confirmed by a strong alteration in the composition of selected organic acids included in the Krebs cycle. The increased ratio between pyruvic acid and citric acid was a very sensitive parameter of phytotoxicity of lichen secondary metabolites to the algal partner of symbiosis. Secondary metabolites of lichens are potent allelochemicals and play significant roles in maintaining the balance between mycobionts and photobionts, forming lichen thallus.},
}
MeSH Terms:
show MeSH Terms
hide MeSH Terms
*Lichens/metabolism/chemistry/growth & development
*Secondary Metabolism
*Allelopathy
Benzofurans/pharmacology/metabolism/chemistry
Chlorophyll A/metabolism
Chlorophyll/metabolism
Ascomycota/metabolism/drug effects
Carotenoids/metabolism
Hydroxybenzoates
RevDate: 2024-10-16
CmpDate: 2024-10-16
The Influence of Rhizobial Nod Factors on the Synthesis of Flavonoids in Common Buckwheat (Fagopyrum esculentum Moench).
Molecules (Basel, Switzerland), 29(19): pii:molecules29194546.
Flavonoids constitute a class of polyphenolic secondary metabolites synthesised mainly by plants and possessing anticancer, antioxidant, anti-inflammatory, and antiviral properties. Common buckwheat (F. esculentum Moench) is a dicotyledonous plant rich in different classes of flavonoids (e.g., rutin) and other phenolic compounds. Lipochitooligosaccharides (LCOs), i.e., rhizobial Nod factors and important signalling molecules for the initiation of symbiosis with legumes, are very effective mitogens that stimulate cell division in plant meristems and the production of secondary metabolites. They can also act in this way in non-legume plants. It has been shown that rhizobial Nod factors noticeably improve plant growth. Rhizobial Nod factors influence the production of flavonoids in common buckwheat grown in greenhouse conditions. The amount of rutin and isoorientin in leaves and flowers has been shown to increase in a statistically significant way after application of Nod factors to buckwheat seeds. The presence of rhizobial Nod factors has no influence on the flavonoid content in stems and roots.
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@article {pmid39407476,
year = {2024},
author = {Kidaj, D and Zamlynska, K and Swatek, A and Komaniecka, I},
title = {The Influence of Rhizobial Nod Factors on the Synthesis of Flavonoids in Common Buckwheat (Fagopyrum esculentum Moench).},
journal = {Molecules (Basel, Switzerland)},
volume = {29},
number = {19},
pages = {},
doi = {10.3390/molecules29194546},
pmid = {39407476},
issn = {1420-3049},
mesh = {*Fagopyrum/metabolism/chemistry ; *Flavonoids/metabolism ; *Rhizobium/metabolism ; Lipopolysaccharides ; Plant Leaves/metabolism/chemistry ; Plant Roots/metabolism ; Flowers/metabolism/chemistry ; Symbiosis ; Seeds/metabolism/chemistry ; },
abstract = {Flavonoids constitute a class of polyphenolic secondary metabolites synthesised mainly by plants and possessing anticancer, antioxidant, anti-inflammatory, and antiviral properties. Common buckwheat (F. esculentum Moench) is a dicotyledonous plant rich in different classes of flavonoids (e.g., rutin) and other phenolic compounds. Lipochitooligosaccharides (LCOs), i.e., rhizobial Nod factors and important signalling molecules for the initiation of symbiosis with legumes, are very effective mitogens that stimulate cell division in plant meristems and the production of secondary metabolites. They can also act in this way in non-legume plants. It has been shown that rhizobial Nod factors noticeably improve plant growth. Rhizobial Nod factors influence the production of flavonoids in common buckwheat grown in greenhouse conditions. The amount of rutin and isoorientin in leaves and flowers has been shown to increase in a statistically significant way after application of Nod factors to buckwheat seeds. The presence of rhizobial Nod factors has no influence on the flavonoid content in stems and roots.},
}
MeSH Terms:
show MeSH Terms
hide MeSH Terms
*Fagopyrum/metabolism/chemistry
*Flavonoids/metabolism
*Rhizobium/metabolism
Lipopolysaccharides
Plant Leaves/metabolism/chemistry
Plant Roots/metabolism
Flowers/metabolism/chemistry
Symbiosis
Seeds/metabolism/chemistry
RevDate: 2024-10-15
CmpDate: 2024-10-16
Unveiling lignocellulolytic potential: a genomic exploration of bacterial lineages within the termite gut.
Microbiome, 12(1):201.
BACKGROUND: The microbial landscape within termite guts varies across termite families. The gut microbiota of lower termites (LT) is dominated by cellulolytic flagellates that sequester wood particles in their digestive vacuoles, whereas in the flagellate-free higher termites (HT), cellulolytic activity has been attributed to fiber-associated bacteria. However, little is known about the role of individual lineages in fiber digestion, particularly in LT.
RESULTS: We investigated the lignocellulolytic potential of 2223 metagenome-assembled genomes (MAGs) recovered from the gut metagenomes of 51 termite species. In the flagellate-dependent LT, cellulolytic enzymes are restricted to MAGs of Bacteroidota (Dysgonomonadaceae, Tannerellaceae, Bacteroidaceae, Azobacteroidaceae) and Spirochaetota (Breznakiellaceae) and reflect a specialization on cellodextrins, whereas their hemicellulolytic arsenal features activities on xylans and diverse heteropolymers. By contrast, the MAGs derived from flagellate-free HT possess a comprehensive arsenal of exo- and endoglucanases that resembles that of termite gut flagellates, underlining that Fibrobacterota and Spirochaetota occupy the cellulolytic niche that became vacant after the loss of the flagellates. Furthermore, we detected directly or indirectly oxygen-dependent enzymes that oxidize cellulose or modify lignin in MAGs of Pseudomonadota (Burkholderiales, Pseudomonadales) and Actinomycetota (Actinomycetales, Mycobacteriales), representing lineages located at the hindgut wall.
CONCLUSIONS: The results of this study refine our concept of symbiotic digestion of lignocellulose in termite guts, emphasizing the differential roles of specific bacterial lineages in both flagellate-dependent and flagellate-independent breakdown of cellulose and hemicelluloses, as well as a so far unappreciated role of oxygen in the depolymerization of plant fiber and lignin in the microoxic periphery during gut passage in HT. Video Abstract.
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@article {pmid39407345,
year = {2024},
author = {Salgado, JFM and Hervé, V and Vera, MAG and Tokuda, G and Brune, A},
title = {Unveiling lignocellulolytic potential: a genomic exploration of bacterial lineages within the termite gut.},
journal = {Microbiome},
volume = {12},
number = {1},
pages = {201},
pmid = {39407345},
issn = {2049-2618},
mesh = {Animals ; *Isoptera/microbiology ; *Lignin/metabolism ; *Gastrointestinal Microbiome ; Phylogeny ; Bacteria/genetics/classification/isolation & purification/enzymology ; Metagenome ; Polysaccharides/metabolism ; Genome, Bacterial ; Genomics ; Cellulose/metabolism ; },
abstract = {BACKGROUND: The microbial landscape within termite guts varies across termite families. The gut microbiota of lower termites (LT) is dominated by cellulolytic flagellates that sequester wood particles in their digestive vacuoles, whereas in the flagellate-free higher termites (HT), cellulolytic activity has been attributed to fiber-associated bacteria. However, little is known about the role of individual lineages in fiber digestion, particularly in LT.
RESULTS: We investigated the lignocellulolytic potential of 2223 metagenome-assembled genomes (MAGs) recovered from the gut metagenomes of 51 termite species. In the flagellate-dependent LT, cellulolytic enzymes are restricted to MAGs of Bacteroidota (Dysgonomonadaceae, Tannerellaceae, Bacteroidaceae, Azobacteroidaceae) and Spirochaetota (Breznakiellaceae) and reflect a specialization on cellodextrins, whereas their hemicellulolytic arsenal features activities on xylans and diverse heteropolymers. By contrast, the MAGs derived from flagellate-free HT possess a comprehensive arsenal of exo- and endoglucanases that resembles that of termite gut flagellates, underlining that Fibrobacterota and Spirochaetota occupy the cellulolytic niche that became vacant after the loss of the flagellates. Furthermore, we detected directly or indirectly oxygen-dependent enzymes that oxidize cellulose or modify lignin in MAGs of Pseudomonadota (Burkholderiales, Pseudomonadales) and Actinomycetota (Actinomycetales, Mycobacteriales), representing lineages located at the hindgut wall.
CONCLUSIONS: The results of this study refine our concept of symbiotic digestion of lignocellulose in termite guts, emphasizing the differential roles of specific bacterial lineages in both flagellate-dependent and flagellate-independent breakdown of cellulose and hemicelluloses, as well as a so far unappreciated role of oxygen in the depolymerization of plant fiber and lignin in the microoxic periphery during gut passage in HT. Video Abstract.},
}
MeSH Terms:
show MeSH Terms
hide MeSH Terms
Animals
*Isoptera/microbiology
*Lignin/metabolism
*Gastrointestinal Microbiome
Phylogeny
Bacteria/genetics/classification/isolation & purification/enzymology
Metagenome
Polysaccharides/metabolism
Genome, Bacterial
Genomics
Cellulose/metabolism
RevDate: 2024-10-17
CmpDate: 2024-10-17
Characterization of the ruminal microbiota in sheep and goats fed different levels of tannin-rich Sericea lespedeza hay.
Journal of animal science, 102:.
Understanding ruminal microbiota and diet-host breed interactions under forage feeding conditions is essential for optimizing rumen fermentation and improving feed efficiency in small ruminants. This study aimed to investigate the effects of different ratios of condensed tannin-rich Sericea lespedeza (SL; Lespedeza cuneata) in the diets on changes and interactions of ruminal microbiota and host species (i.e., sheep and goats). Katahdin sheep (n = 12) and Alpine goats (n = 12) at approximately 10 to 12 mo of age were blocked by body weight (BW = 30.3 and 25.5 kg, respectively) and randomly assigned to one of the 3 treatments. Diets contained 75% coarsely ground forage and 25% concentrate. The forages were 1) 100% alfalfa hay (AL), 2) 100% SL, and 3) 50% AL + 50% SL (ASL). In the present study, the diversity and composition of ruminal microbiota differed between sheep and goats fed similar diets. Based on the taxonomic analysis, there was a distinct clustering pattern (P < 0.05) for sheep by diets, but such a pattern was not observed for goats (P > 0.1). The most predominant phyla were Firmicutes, Bacteroidetes, Ascomycota, and methanogen species of Methanobrevibactor sp. in the rumen of sheep and goats, regardless of diets. The Bacteroidetes and Ascomycota were enriched in sheep fed AL and ASL. In contrast, these microbial phyla were enhanced in goats fed tannin-rich SL diets, with the diet-by-host species interaction (P < 0.02) for the Bacteroidetes phylum. Sheep rumen fluid samples showed a higher degree of variability in microbial community composition compared to goat rumen fluid samples. The relative proportion of the Aspergillus fungi population was reduced to 90.7% in the SL group compared with the AL group, regardless of host species. The antimicrobial activity of tannins and greater sensitivities of selected microbiota species to these tannin compounds during SL feeding in sheep and goats perhaps caused this difference. The results from this study suggest that differences in the microbiota were associated with differences in diets and host species. Therefore, this study provides a better understanding of ruminal microbiota and diet-host species interactions under various tannin-rich diets, which could advance consolidative information on rumen microbiome community diversity changes and may improve sheep and goat production.
Additional Links: PMID-39018107
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@article {pmid39018107,
year = {2024},
author = {Min, BR and Wang, W and Pitta, DW and Indugu, N and Patra, AK and Wang, HH and Abrahamsen, F and Hilaire, M and Puchala, R},
title = {Characterization of the ruminal microbiota in sheep and goats fed different levels of tannin-rich Sericea lespedeza hay.},
journal = {Journal of animal science},
volume = {102},
number = {},
pages = {},
pmid = {39018107},
issn = {1525-3163},
mesh = {Animals ; *Goats ; *Rumen/microbiology ; *Diet/veterinary ; *Animal Feed/analysis ; Sheep/microbiology ; *Tannins/pharmacology/chemistry ; *Gastrointestinal Microbiome/drug effects ; Lespedeza/chemistry ; Animal Nutritional Physiological Phenomena ; Male ; Random Allocation ; Fermentation ; },
abstract = {Understanding ruminal microbiota and diet-host breed interactions under forage feeding conditions is essential for optimizing rumen fermentation and improving feed efficiency in small ruminants. This study aimed to investigate the effects of different ratios of condensed tannin-rich Sericea lespedeza (SL; Lespedeza cuneata) in the diets on changes and interactions of ruminal microbiota and host species (i.e., sheep and goats). Katahdin sheep (n = 12) and Alpine goats (n = 12) at approximately 10 to 12 mo of age were blocked by body weight (BW = 30.3 and 25.5 kg, respectively) and randomly assigned to one of the 3 treatments. Diets contained 75% coarsely ground forage and 25% concentrate. The forages were 1) 100% alfalfa hay (AL), 2) 100% SL, and 3) 50% AL + 50% SL (ASL). In the present study, the diversity and composition of ruminal microbiota differed between sheep and goats fed similar diets. Based on the taxonomic analysis, there was a distinct clustering pattern (P < 0.05) for sheep by diets, but such a pattern was not observed for goats (P > 0.1). The most predominant phyla were Firmicutes, Bacteroidetes, Ascomycota, and methanogen species of Methanobrevibactor sp. in the rumen of sheep and goats, regardless of diets. The Bacteroidetes and Ascomycota were enriched in sheep fed AL and ASL. In contrast, these microbial phyla were enhanced in goats fed tannin-rich SL diets, with the diet-by-host species interaction (P < 0.02) for the Bacteroidetes phylum. Sheep rumen fluid samples showed a higher degree of variability in microbial community composition compared to goat rumen fluid samples. The relative proportion of the Aspergillus fungi population was reduced to 90.7% in the SL group compared with the AL group, regardless of host species. The antimicrobial activity of tannins and greater sensitivities of selected microbiota species to these tannin compounds during SL feeding in sheep and goats perhaps caused this difference. The results from this study suggest that differences in the microbiota were associated with differences in diets and host species. Therefore, this study provides a better understanding of ruminal microbiota and diet-host species interactions under various tannin-rich diets, which could advance consolidative information on rumen microbiome community diversity changes and may improve sheep and goat production.},
}
MeSH Terms:
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Animals
*Goats
*Rumen/microbiology
*Diet/veterinary
*Animal Feed/analysis
Sheep/microbiology
*Tannins/pharmacology/chemistry
*Gastrointestinal Microbiome/drug effects
Lespedeza/chemistry
Animal Nutritional Physiological Phenomena
Male
Random Allocation
Fermentation
RevDate: 2024-10-15
CmpDate: 2024-10-16
An iron-binding protein of entomopathogenic fungus suppresses the proliferation of host symbiotic bacteria.
Microbiome, 12(1):202.
BACKGROUND: Entomopathogenic fungal infection-induced dysbiosis of host microbiota offers a window into understanding the complex interactions between pathogenic fungi and host symbionts. Such insights are critical for enhancing the efficacy of mycoinsecticides. However, the utilization of these interactions in pest control remains largely unexplored.
RESULTS: Here, we found that infection by the host-specialist fungus Metarhizium acridum alters the composition of the symbiotic microbiota and increases the dominance of some bacterial symbionts in locusts. Meanwhile, M. acridum also effectively limits the overgrowth of the predominant bacteria. Comparative transcriptomic screening revealed that the fungus upregulates the production of MaCFEM1, an iron-binding protein, in the presence of bacteria. This protein sequesters iron, thereby limiting its availability. Functionally, overexpression of MaCFEM1 in the fungus induces iron deprivation, which significantly suppresses bacterial growth. Conversely, MaCFEM1 knockout relieves the restriction on bacterial iron availability, resulting in iron reallocation. Upon ΔMaCFEM1 infection, some host bacterial symbionts proliferate uncontrollably, turning into opportunistic pathogens and significantly accelerating host death.
CONCLUSIONS: This study elucidates the critical role of pathogenic fungal-dominated iron allocation in mediating the shift of host microbes from symbiosis to pathogenicity. It also highlights a unique biocontrol strategy that jointly exploits pathogenic fungi and bacterial symbionts to increase host mortality. Video Abstract.
Additional Links: PMID-39407320
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@article {pmid39407320,
year = {2024},
author = {Li, J and Li, J and Cao, L and Chen, Q and Ding, D and Kang, L},
title = {An iron-binding protein of entomopathogenic fungus suppresses the proliferation of host symbiotic bacteria.},
journal = {Microbiome},
volume = {12},
number = {1},
pages = {202},
pmid = {39407320},
issn = {2049-2618},
mesh = {*Symbiosis ; *Iron/metabolism ; Animals ; *Metarhizium/genetics/metabolism/pathogenicity/physiology ; *Fungal Proteins/genetics/metabolism ; *Bacteria/metabolism/genetics/classification ; Grasshoppers/microbiology ; Host-Pathogen Interactions ; Dysbiosis/microbiology ; },
abstract = {BACKGROUND: Entomopathogenic fungal infection-induced dysbiosis of host microbiota offers a window into understanding the complex interactions between pathogenic fungi and host symbionts. Such insights are critical for enhancing the efficacy of mycoinsecticides. However, the utilization of these interactions in pest control remains largely unexplored.
RESULTS: Here, we found that infection by the host-specialist fungus Metarhizium acridum alters the composition of the symbiotic microbiota and increases the dominance of some bacterial symbionts in locusts. Meanwhile, M. acridum also effectively limits the overgrowth of the predominant bacteria. Comparative transcriptomic screening revealed that the fungus upregulates the production of MaCFEM1, an iron-binding protein, in the presence of bacteria. This protein sequesters iron, thereby limiting its availability. Functionally, overexpression of MaCFEM1 in the fungus induces iron deprivation, which significantly suppresses bacterial growth. Conversely, MaCFEM1 knockout relieves the restriction on bacterial iron availability, resulting in iron reallocation. Upon ΔMaCFEM1 infection, some host bacterial symbionts proliferate uncontrollably, turning into opportunistic pathogens and significantly accelerating host death.
CONCLUSIONS: This study elucidates the critical role of pathogenic fungal-dominated iron allocation in mediating the shift of host microbes from symbiosis to pathogenicity. It also highlights a unique biocontrol strategy that jointly exploits pathogenic fungi and bacterial symbionts to increase host mortality. Video Abstract.},
}
MeSH Terms:
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*Symbiosis
*Iron/metabolism
Animals
*Metarhizium/genetics/metabolism/pathogenicity/physiology
*Fungal Proteins/genetics/metabolism
*Bacteria/metabolism/genetics/classification
Grasshoppers/microbiology
Host-Pathogen Interactions
Dysbiosis/microbiology
RevDate: 2024-10-15
CmpDate: 2024-10-15
Prior heat stress increases pathogen susceptibility in the model cnidarian Exaiptasia diaphana.
Communications biology, 7(1):1328.
Anthropogenic climate change has significantly altered terrestrial and marine ecosystems globally, often in the form of climate-related events such as thermal anomalies and disease outbreaks. Although the isolated effects of these stressors have been well documented, a growing body of literature suggests that stressors often interact, resulting in complex effects on ecosystems. This includes coral reefs where sequential associations between heat stress and disease have had profound impacts. Here we used the model cnidarian Exaiptasia diaphana to investigate mechanisms linking prior heat stress to increased disease susceptibility. We examined anemone pathogen susceptibility and physiology (symbiosis, immunity, and energetics) following recovery from heat stress. We observed significantly increased pathogen susceptibility in anemones previously exposed to heat stress. Notably, prior heat stress reduced anemone energetic reserves (carbohydrate concentration), and activity of multiple immune components. Minimal effects of prior heat stress on symbiont density were observed. Together, results suggest changes in energetic availability might have the strongest effect on pathogen susceptibility and immunity following heat stress. The results presented here provide critical insight regarding the interplay between heat stress recovery and pathogen susceptibility in cnidarians and are an important first step towards understanding temporal associations between these stressors.
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@article {pmid39406834,
year = {2024},
author = {Diaz de Villegas, SC and Borbee, EM and Abdelbaki, PY and Fuess, LE},
title = {Prior heat stress increases pathogen susceptibility in the model cnidarian Exaiptasia diaphana.},
journal = {Communications biology},
volume = {7},
number = {1},
pages = {1328},
pmid = {39406834},
issn = {2399-3642},
support = {Startup Funding//Texas State University/ ; Gulf Research Early Career Fellowship//National Academies of Sciences, Engineering, and Medicine | National Academy of Engineering (NAE)/ ; PRFB 2209205//National Science Foundation (NSF)/ ; },
mesh = {Animals ; *Sea Anemones/physiology ; *Heat-Shock Response ; Disease Susceptibility ; Symbiosis ; Climate Change ; },
abstract = {Anthropogenic climate change has significantly altered terrestrial and marine ecosystems globally, often in the form of climate-related events such as thermal anomalies and disease outbreaks. Although the isolated effects of these stressors have been well documented, a growing body of literature suggests that stressors often interact, resulting in complex effects on ecosystems. This includes coral reefs where sequential associations between heat stress and disease have had profound impacts. Here we used the model cnidarian Exaiptasia diaphana to investigate mechanisms linking prior heat stress to increased disease susceptibility. We examined anemone pathogen susceptibility and physiology (symbiosis, immunity, and energetics) following recovery from heat stress. We observed significantly increased pathogen susceptibility in anemones previously exposed to heat stress. Notably, prior heat stress reduced anemone energetic reserves (carbohydrate concentration), and activity of multiple immune components. Minimal effects of prior heat stress on symbiont density were observed. Together, results suggest changes in energetic availability might have the strongest effect on pathogen susceptibility and immunity following heat stress. The results presented here provide critical insight regarding the interplay between heat stress recovery and pathogen susceptibility in cnidarians and are an important first step towards understanding temporal associations between these stressors.},
}
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Animals
*Sea Anemones/physiology
*Heat-Shock Response
Disease Susceptibility
Symbiosis
Climate Change
RevDate: 2024-10-15
Plastiskin: A new form of plastic pollution affecting rocky shore organisms.
Marine pollution bulletin, 209(Pt A):117121 pii:S0025-326X(24)01098-1 [Epub ahead of print].
Plastic pollution has become a significant environmental concern, with profound consequences for ecosystems worldwide, particularly for marine systems. Our study introduces 'plastiskin', a newly identified plastic pollution type encrusting intertidal organisms. Found on mussels and macroalgae, 'plastiskin' was composed of polypropylene and polyethylene. In mussels, the presence of 'plastiskin' was correlated with the absence of living endoliths in areas of the shells it covered, indicating a detrimental impact on the symbiotic endolithic community residing within mussel shells. In addition, we examined the potential negative effects of 'plastiskin' on the photosynthetic efficiency of macroalgae, however, these findings were inconclusive, stressing the need for further studies with larger sample sizes. Our baseline observations may serve as a groundwork for further investigation into the spatial distribution, temporal persistence, and ecological ramifications of 'plastiskin'. potential incorporation of 'plastiskin' as a new marine debris category into management and monitoring frameworks warrants serious consideration.
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@article {pmid39406061,
year = {2024},
author = {Zardi, GI and Seuront, L and Gevaert, F and Nicastro, KR},
title = {Plastiskin: A new form of plastic pollution affecting rocky shore organisms.},
journal = {Marine pollution bulletin},
volume = {209},
number = {Pt A},
pages = {117121},
doi = {10.1016/j.marpolbul.2024.117121},
pmid = {39406061},
issn = {1879-3363},
abstract = {Plastic pollution has become a significant environmental concern, with profound consequences for ecosystems worldwide, particularly for marine systems. Our study introduces 'plastiskin', a newly identified plastic pollution type encrusting intertidal organisms. Found on mussels and macroalgae, 'plastiskin' was composed of polypropylene and polyethylene. In mussels, the presence of 'plastiskin' was correlated with the absence of living endoliths in areas of the shells it covered, indicating a detrimental impact on the symbiotic endolithic community residing within mussel shells. In addition, we examined the potential negative effects of 'plastiskin' on the photosynthetic efficiency of macroalgae, however, these findings were inconclusive, stressing the need for further studies with larger sample sizes. Our baseline observations may serve as a groundwork for further investigation into the spatial distribution, temporal persistence, and ecological ramifications of 'plastiskin'. potential incorporation of 'plastiskin' as a new marine debris category into management and monitoring frameworks warrants serious consideration.},
}
RevDate: 2024-10-15
Preinoculation with Bradyrhizobium japonicum enhances the salt tolerance of Glycine max seedlings by regulating polyamine metabolism in roots.
Plant physiology and biochemistry : PPB, 216:109196 pii:S0981-9428(24)00864-7 [Epub ahead of print].
Rhizobia are common symbiotic microorganisms in the root system of leguminous plants that can usually provide nitrogen to the host through nitrogen fixation. Studies have shown that rhizobium-preinoculated soybean plants usually exhibit improved salt tolerance, but the underlying mechanism is not fully understood. In this paper, transcriptome sequencing (RNA-seq) revealed that preinoculation with rhizobia affected polyamine (PA) metabolism in soybean roots. The assay of PA contents showed that preinoculation with rhizobia significantly increased the putrescine (Put) content in roots and leaves during short-term salt treatment (0-5 d). Long-term salt treatment (5-7 d) resulted in a high Put content and significantly increased Spm and Spd contents, resulting in a rapid increase in the Put/(Spd + Spm) ratio (0-5 d) and subsequent decrease. Moreover, rhizobium preinoculation of soybean plants resulted in increased contents of conjugated and bound PAs under salt stress. Further transcriptome sequencing, PA contents, PA synthase expression and activity analysis revealed that GmADC may be a key gene related to salt tolerance in rhizobium-preinoculated soybean plants, and the GmADC-overexpressing soybean hairy-root composite plants exhibited less ROS damage, lower Cl[-]/NO3[-] ratios and Na[+]/K[+] ratios, and stabilized ion homeostasis. Taken together, preinoculation with rhizobia increased the expression level and enzyme activity of arginine decarboxylase (ADC) in soybean roots, increased the content of Put in roots and leaves, and increased the content of conjugated and bound PAs in soybean plants, thereby alleviating the oxidative and ionic injuries of soybean plants and enhancing the salt tolerance.
Additional Links: PMID-39405999
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@article {pmid39405999,
year = {2024},
author = {Li, C and Huang, Q and Sun, S and Cheng, C and Chen, Y and Yu, B},
title = {Preinoculation with Bradyrhizobium japonicum enhances the salt tolerance of Glycine max seedlings by regulating polyamine metabolism in roots.},
journal = {Plant physiology and biochemistry : PPB},
volume = {216},
number = {},
pages = {109196},
doi = {10.1016/j.plaphy.2024.109196},
pmid = {39405999},
issn = {1873-2690},
abstract = {Rhizobia are common symbiotic microorganisms in the root system of leguminous plants that can usually provide nitrogen to the host through nitrogen fixation. Studies have shown that rhizobium-preinoculated soybean plants usually exhibit improved salt tolerance, but the underlying mechanism is not fully understood. In this paper, transcriptome sequencing (RNA-seq) revealed that preinoculation with rhizobia affected polyamine (PA) metabolism in soybean roots. The assay of PA contents showed that preinoculation with rhizobia significantly increased the putrescine (Put) content in roots and leaves during short-term salt treatment (0-5 d). Long-term salt treatment (5-7 d) resulted in a high Put content and significantly increased Spm and Spd contents, resulting in a rapid increase in the Put/(Spd + Spm) ratio (0-5 d) and subsequent decrease. Moreover, rhizobium preinoculation of soybean plants resulted in increased contents of conjugated and bound PAs under salt stress. Further transcriptome sequencing, PA contents, PA synthase expression and activity analysis revealed that GmADC may be a key gene related to salt tolerance in rhizobium-preinoculated soybean plants, and the GmADC-overexpressing soybean hairy-root composite plants exhibited less ROS damage, lower Cl[-]/NO3[-] ratios and Na[+]/K[+] ratios, and stabilized ion homeostasis. Taken together, preinoculation with rhizobia increased the expression level and enzyme activity of arginine decarboxylase (ADC) in soybean roots, increased the content of Put in roots and leaves, and increased the content of conjugated and bound PAs in soybean plants, thereby alleviating the oxidative and ionic injuries of soybean plants and enhancing the salt tolerance.},
}
RevDate: 2024-10-15
Deciphering defense system modulating bacteria-mobile genetic elements symbiosis in microbial aggregates under elevated hydraulic stress.
Water research, 268(Pt A):122590 pii:S0043-1354(24)01489-1 [Epub ahead of print].
Bacterial defense systems are under strong evolutionary pressures to defend against mobile genetic elements (MGEs), yet their distribution in microbial aggregates in engineered systems remains largely unexplored. Herein, we investigated the bacterial defensome and MGEs within activated sludge flocs (AS) and membrane-attached biofilm (MF) in a full-scale membrane bioreactor. Similar distribution pattern of bacterial defense systems (63 types) was observed in prokaryotic genome in AS and MF, including RM system (∼40 %), Cas system (∼18 %) and TA-Abi system (∼28 %), exhibiting a dependency on the genome size and bacterial taxonomy in microbial aggregates under elevated hydraulic stress (MF). In contrast to plasmid and provirus, which carried defense systems (22 types) similar to their associated hosts, virome (61 %) carried novel defense systems (40 types) absent in their associated hosts. With 54 % of which involved in MGEs geneflow network, 69 % of high quality bacterial genome bins were associated with horizontal gene transfer (HGT), facilitating the exchange of mobile core functional genes. This potentially conferred competitive advantages to hosts through habitat-specific payload genes related to biotic defense, antibiotic resistance, and nitrogen metabolism. The longer growth cycle and varied defense gene density suggested the potential defense redundancy and trade-off of metabolic expense and immunity in bacterial host-MGE symbionts. Furthermore, enhanced cooperative network modules of cross-feeding and defense were observed in the MF, potentially helped the symbiotic microbial communities in coping with hostile conditions under elevated hydraulic stress. These findings shed light on the dynamics of bacterial defense systems in host-MGE coevolution and provide new perspectives of microbial aggregates manipulation for ecological and engineering application.
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@article {pmid39405618,
year = {2024},
author = {Tan, Y and Yu, P and Yu, Z and Xuan, F and Zhu, L},
title = {Deciphering defense system modulating bacteria-mobile genetic elements symbiosis in microbial aggregates under elevated hydraulic stress.},
journal = {Water research},
volume = {268},
number = {Pt A},
pages = {122590},
doi = {10.1016/j.watres.2024.122590},
pmid = {39405618},
issn = {1879-2448},
abstract = {Bacterial defense systems are under strong evolutionary pressures to defend against mobile genetic elements (MGEs), yet their distribution in microbial aggregates in engineered systems remains largely unexplored. Herein, we investigated the bacterial defensome and MGEs within activated sludge flocs (AS) and membrane-attached biofilm (MF) in a full-scale membrane bioreactor. Similar distribution pattern of bacterial defense systems (63 types) was observed in prokaryotic genome in AS and MF, including RM system (∼40 %), Cas system (∼18 %) and TA-Abi system (∼28 %), exhibiting a dependency on the genome size and bacterial taxonomy in microbial aggregates under elevated hydraulic stress (MF). In contrast to plasmid and provirus, which carried defense systems (22 types) similar to their associated hosts, virome (61 %) carried novel defense systems (40 types) absent in their associated hosts. With 54 % of which involved in MGEs geneflow network, 69 % of high quality bacterial genome bins were associated with horizontal gene transfer (HGT), facilitating the exchange of mobile core functional genes. This potentially conferred competitive advantages to hosts through habitat-specific payload genes related to biotic defense, antibiotic resistance, and nitrogen metabolism. The longer growth cycle and varied defense gene density suggested the potential defense redundancy and trade-off of metabolic expense and immunity in bacterial host-MGE symbionts. Furthermore, enhanced cooperative network modules of cross-feeding and defense were observed in the MF, potentially helped the symbiotic microbial communities in coping with hostile conditions under elevated hydraulic stress. These findings shed light on the dynamics of bacterial defense systems in host-MGE coevolution and provide new perspectives of microbial aggregates manipulation for ecological and engineering application.},
}
RevDate: 2024-10-15
CmpDate: 2024-10-15
Variation in SSRs at different genomic regions and implications for the evolution and identification of Armillaria gallica.
PloS one, 19(10):e0312114.
Armillaria spp. are devastating forest pathogens. Due to its low pathogenicity and abundant genetic variation, Armillaria gallica exhibited a unique and beneficial symbiosis with Gastrodia elata, which was used as a traditional Chinese medicine. However, the variation and population structure of A. gallica populations have rarely been investigated. Hence, we analyzed the evolution and variation in simple sequence repeats (SSRs) in three Armillaria genomes: A. gallica, A. cepistipes, and A. ostoyae to assess the genetic diversity and population structure of 14 A. gallica strains. Genome analysis revealed that SSRs were more abundant in the intergenic region than the intron and exon region, as was the SSR density. Compared with other two genomes, SSR density was the lowest in exon region and largest in the intron region of A. gallica, with significant variation in genic region. There were 17 polymorphic markers in A. gallica genome was identified, with 26.7% in genic region, which is higher than that of 18.8% in the intergenic region. Moreover, a total of 50 alleles and 42 polymorphic loci were detected among these A. gallica strains. The averaged polymorphism information content (PIC) was 0.4487, ranged from 0.2577 to 0.6786. Both principal coordinate analysis (PCoA) and population structure analyses based on the genotype data of SSRs divided the strains into two clusters. The cluster I included all the strains from high-altitude G. elata producing areas and some low-altitude areas, while the strains in Cluster II originated from low-altitude G. elata producing areas. These results indicated that substantial genome-specific variation in SSRs within the genic region of A. gallica and provide new insights for further studies on the evolution and breeding of A. gallica.
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@article {pmid39405284,
year = {2024},
author = {Li, S and Xu, J and Hu, Y and Ou, X and Yuan, Q and Li, P and Jiang, W and Guo, L and Zhou, T},
title = {Variation in SSRs at different genomic regions and implications for the evolution and identification of Armillaria gallica.},
journal = {PloS one},
volume = {19},
number = {10},
pages = {e0312114},
pmid = {39405284},
issn = {1932-6203},
mesh = {*Microsatellite Repeats/genetics ; *Armillaria/genetics ; Phylogeny ; Genome, Plant ; Evolution, Molecular ; Polymorphism, Genetic ; Genetic Variation ; },
abstract = {Armillaria spp. are devastating forest pathogens. Due to its low pathogenicity and abundant genetic variation, Armillaria gallica exhibited a unique and beneficial symbiosis with Gastrodia elata, which was used as a traditional Chinese medicine. However, the variation and population structure of A. gallica populations have rarely been investigated. Hence, we analyzed the evolution and variation in simple sequence repeats (SSRs) in three Armillaria genomes: A. gallica, A. cepistipes, and A. ostoyae to assess the genetic diversity and population structure of 14 A. gallica strains. Genome analysis revealed that SSRs were more abundant in the intergenic region than the intron and exon region, as was the SSR density. Compared with other two genomes, SSR density was the lowest in exon region and largest in the intron region of A. gallica, with significant variation in genic region. There were 17 polymorphic markers in A. gallica genome was identified, with 26.7% in genic region, which is higher than that of 18.8% in the intergenic region. Moreover, a total of 50 alleles and 42 polymorphic loci were detected among these A. gallica strains. The averaged polymorphism information content (PIC) was 0.4487, ranged from 0.2577 to 0.6786. Both principal coordinate analysis (PCoA) and population structure analyses based on the genotype data of SSRs divided the strains into two clusters. The cluster I included all the strains from high-altitude G. elata producing areas and some low-altitude areas, while the strains in Cluster II originated from low-altitude G. elata producing areas. These results indicated that substantial genome-specific variation in SSRs within the genic region of A. gallica and provide new insights for further studies on the evolution and breeding of A. gallica.},
}
MeSH Terms:
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*Microsatellite Repeats/genetics
*Armillaria/genetics
Phylogeny
Genome, Plant
Evolution, Molecular
Polymorphism, Genetic
Genetic Variation
RevDate: 2024-10-16
CmpDate: 2024-10-14
Escherichia coli on colorectal cancer: A two-edged sword.
Microbial biotechnology, 17(10):e70029.
Escherichia coli (E. coli) is a ubiquitous symbiotic bacterium in the gut, and the diversity of E. coli genes determines the diversity of its functions. In this review, the two-edged sword theory was innovatively proposed. For the question 'how can we harness the ambivalent nature of E. coli to screen and treat CRC?', in terms of CRC screening, the variations in the abundance and subtypes of E. coli across different populations present an opportunity to utilise it as a biomarker, while in terms of CRC treatment, the natural beneficial effect of E. coli on CRC may be limited, and engineered E. coli, particularly certain subtypes with probiotic potential, can indeed play a significant role in CRC treatment. It seems that the favourable role of E. coli as a genetic tool lies not in its direct impact on CRC but its potential as a research platform that can be integrated with various technologies such as nanoparticles, imaging methods, and synthetic biology modification. The relationship between gut microflora and CRC remains unclear due to the complex diversity and interaction of gut microflora. Therefore, the application of E. coli should be based on the 'One Health' view and take the interactions between E. coli and other microorganisms, host, and environmental factors, as well as its own changes into account. In this paper, the two-edged sword role of E. coli in CRC is emphasised to realise the great potential of E. coli in CRC screening and treatment.
Additional Links: PMID-39400440
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@article {pmid39400440,
year = {2024},
author = {Jian, C and Yinhang, W and Jing, Z and Zhanbo, Q and Zefeng, W and Shuwen, H},
title = {Escherichia coli on colorectal cancer: A two-edged sword.},
journal = {Microbial biotechnology},
volume = {17},
number = {10},
pages = {e70029},
pmid = {39400440},
issn = {1751-7915},
support = {2023GZ86//Public Welfare Technology Application Research Program of Huzhou/ ; 2023HT078//China University Industry University Research Innovation Fund/ ; 2023GY04//Medical Science and Technology Project of Zhejiang Province/ ; },
mesh = {*Colorectal Neoplasms/microbiology ; *Escherichia coli/genetics ; Humans ; *Gastrointestinal Microbiome ; Probiotics ; },
abstract = {Escherichia coli (E. coli) is a ubiquitous symbiotic bacterium in the gut, and the diversity of E. coli genes determines the diversity of its functions. In this review, the two-edged sword theory was innovatively proposed. For the question 'how can we harness the ambivalent nature of E. coli to screen and treat CRC?', in terms of CRC screening, the variations in the abundance and subtypes of E. coli across different populations present an opportunity to utilise it as a biomarker, while in terms of CRC treatment, the natural beneficial effect of E. coli on CRC may be limited, and engineered E. coli, particularly certain subtypes with probiotic potential, can indeed play a significant role in CRC treatment. It seems that the favourable role of E. coli as a genetic tool lies not in its direct impact on CRC but its potential as a research platform that can be integrated with various technologies such as nanoparticles, imaging methods, and synthetic biology modification. The relationship between gut microflora and CRC remains unclear due to the complex diversity and interaction of gut microflora. Therefore, the application of E. coli should be based on the 'One Health' view and take the interactions between E. coli and other microorganisms, host, and environmental factors, as well as its own changes into account. In this paper, the two-edged sword role of E. coli in CRC is emphasised to realise the great potential of E. coli in CRC screening and treatment.},
}
MeSH Terms:
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*Colorectal Neoplasms/microbiology
*Escherichia coli/genetics
Humans
*Gastrointestinal Microbiome
Probiotics
RevDate: 2024-10-14
Urbanization drives partner switching and loss of mutualism in an ant-plant symbiosis.
Ecology [Epub ahead of print].
Mutualistic interactions between species underpin biodiversity and ecosystem function, but may be lost when partners respond differently to abiotic conditions. Except for a few prominent examples, effects of global anthropogenic change on mutualisms are poorly understood. Here we assess the effects of urbanization on a symbiosis in which the plant Cordia nodosa house ants in hollow structures (domatia) in exchange for defense against herbivores. We expected to find that mutualist ants would be replaced in the city by heat-tolerant opportunists, leaving urban plants vulnerable to herbivory. In five protected forest sites and five urban forest fragments in southeast Perú, we recorded the identity and heat tolerance (CTmax) of ant residents of C. nodosa. We also assayed their plant-defensive behaviors and their effects on herbivory. We characterized the urban heat-island effect in ambient temperatures and within domatia. Forest plants housed a consistent ant community dominated by three specialized plant ants, whereas urban plants housed a suite of 10 opportunistic taxa that were, collectively, about 13 times less likely than forest ants to respond defensively to plant disturbance. In the forest, ant exclusion had the expected effect of increasing herbivory, but in urban sites, exclusion reduced herbivory. Despite poor ant defense in urban sites, we detected no difference in total standing herbivory, perhaps because herbivores themselves also declined in the city. Urban sites were warmer than forest sites (daily maxima in urban domatia averaged 1.6°C hotter), and the urban ant community as a whole was slightly more heat tolerant. These results illustrate a case of mutualism loss associated with anthropogenic disturbance. If urbanization is representative of increasing anthropogenic stressors more broadly, we might expect to see destabilization of myrmecophytic mutualisms in forest ecosystems in the future.
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@article {pmid39400307,
year = {2024},
author = {Youngsteadt, E and Prado, SG and Duran Aquino, AK and Peña Valdeiglesias, J and Gonzales Ojeda, T and Garate Quispe, JS},
title = {Urbanization drives partner switching and loss of mutualism in an ant-plant symbiosis.},
journal = {Ecology},
volume = {},
number = {},
pages = {e4449},
doi = {10.1002/ecy.4449},
pmid = {39400307},
issn = {1939-9170},
support = {059-2021-FONDECYT//Fondo Nacional de Desarrollo Científico, Tecnológico y de Innovación Tecnológica/ ; //North Carolina State University Internationalization Seed Grant/ ; },
abstract = {Mutualistic interactions between species underpin biodiversity and ecosystem function, but may be lost when partners respond differently to abiotic conditions. Except for a few prominent examples, effects of global anthropogenic change on mutualisms are poorly understood. Here we assess the effects of urbanization on a symbiosis in which the plant Cordia nodosa house ants in hollow structures (domatia) in exchange for defense against herbivores. We expected to find that mutualist ants would be replaced in the city by heat-tolerant opportunists, leaving urban plants vulnerable to herbivory. In five protected forest sites and five urban forest fragments in southeast Perú, we recorded the identity and heat tolerance (CTmax) of ant residents of C. nodosa. We also assayed their plant-defensive behaviors and their effects on herbivory. We characterized the urban heat-island effect in ambient temperatures and within domatia. Forest plants housed a consistent ant community dominated by three specialized plant ants, whereas urban plants housed a suite of 10 opportunistic taxa that were, collectively, about 13 times less likely than forest ants to respond defensively to plant disturbance. In the forest, ant exclusion had the expected effect of increasing herbivory, but in urban sites, exclusion reduced herbivory. Despite poor ant defense in urban sites, we detected no difference in total standing herbivory, perhaps because herbivores themselves also declined in the city. Urban sites were warmer than forest sites (daily maxima in urban domatia averaged 1.6°C hotter), and the urban ant community as a whole was slightly more heat tolerant. These results illustrate a case of mutualism loss associated with anthropogenic disturbance. If urbanization is representative of increasing anthropogenic stressors more broadly, we might expect to see destabilization of myrmecophytic mutualisms in forest ecosystems in the future.},
}
RevDate: 2024-10-14
Dynamic microbiome diversity shaping the adaptation of sponge holobionts in coastal waters.
Microbiology spectrum [Epub ahead of print].
UNLABELLED: The microbial communities associated with sponges contribute to the adaptation of hosts to environments, which are essential for the trophic transformation of benthic-marine coupling. However, little is known about the symbiotic microbial community interactions and adaptative strategies of high- and low-microbial abundance (HMA and LMA) sponges, which represent two typical ecological phenotypes. Here, we compared the 1-year dynamic patterns of microbiomes with the HMA sponge Spongia officinalis and two LMA sponge species Tedania sp. and Haliclona simulans widespread on the coast of China. Symbiotic bacterial communities with the characteristic HMA-LMA dichotomy presented higher diversity and stability in S. officinalis than in Tedania sp. and H. simulans, while archaeal communities showed consistent diversity across all sponges throughout the year. Dissolved oxygen, dissolved inorganic phosphorus, dissolved organic phosphorus, and especially temperature were the major factors affecting the seasonal changes in sponge microbial communities. S. officinalis-associated microbiome had higher diversity, stronger stability, and closer interaction, which adopted a relatively isolated strategy to cope with environmental changes, while Tedania sp. and H. simulans were more susceptible and shared more bacterial Amplicon Sequence Variants (ASVs) with surrounding waters, with an open way facing the uncertainty of the environment. Meta-analysis of the microbiome in composition, diversity, and ecological function from 13 marine sponges further supported that bacterial communities associated with HMA and LMA sponges have evolved two distinct environmental adaptation strategies. We propose that the different adaptive ways of sponges responding to the environment may be responsible for their successful evolution and their competence in global ocean change.
IMPORTANCE: During long-term evolution, sponge holobionts, among the oldest symbiotic relationships between microbes and metazoans, developed two distinct phenotypes with high- and low-microbial abundance (HMA and LMA). Despite sporadic studies indicating that the characteristic microbial assemblages present in HMA and LMA sponges, the adaptation strategies of symbionts responding to environments are still unclear. This deficiency limits our understanding of the selection of symbionts and the ecological functions during the evolutionary history and the adaptative assessment of HMA and LMA sponges in variable environments. Here, we explored symbiotic communities with two distinct phenotypes in a 1-year dynamic environment and combined with the meta-analysis of 13 sponges. The different strategies of symbionts in adapting to the environment were basically drawn: microbes with LMA were more acclimated to environmental changes, forming relatively loose-connected communities, while HMA developed relatively tight-connected and more similar communities beyond the divergence of species and geographical location.
Additional Links: PMID-39400157
Publisher:
PubMed:
Citation:
show bibtex listing
hide bibtex listing
@article {pmid39400157,
year = {2024},
author = {Gan, B and Wang, K and Zhang, B and Jia, C and Lin, X and Zhao, J and Ding, S},
title = {Dynamic microbiome diversity shaping the adaptation of sponge holobionts in coastal waters.},
journal = {Microbiology spectrum},
volume = {},
number = {},
pages = {e0144824},
doi = {10.1128/spectrum.01448-24},
pmid = {39400157},
issn = {2165-0497},
abstract = {UNLABELLED: The microbial communities associated with sponges contribute to the adaptation of hosts to environments, which are essential for the trophic transformation of benthic-marine coupling. However, little is known about the symbiotic microbial community interactions and adaptative strategies of high- and low-microbial abundance (HMA and LMA) sponges, which represent two typical ecological phenotypes. Here, we compared the 1-year dynamic patterns of microbiomes with the HMA sponge Spongia officinalis and two LMA sponge species Tedania sp. and Haliclona simulans widespread on the coast of China. Symbiotic bacterial communities with the characteristic HMA-LMA dichotomy presented higher diversity and stability in S. officinalis than in Tedania sp. and H. simulans, while archaeal communities showed consistent diversity across all sponges throughout the year. Dissolved oxygen, dissolved inorganic phosphorus, dissolved organic phosphorus, and especially temperature were the major factors affecting the seasonal changes in sponge microbial communities. S. officinalis-associated microbiome had higher diversity, stronger stability, and closer interaction, which adopted a relatively isolated strategy to cope with environmental changes, while Tedania sp. and H. simulans were more susceptible and shared more bacterial Amplicon Sequence Variants (ASVs) with surrounding waters, with an open way facing the uncertainty of the environment. Meta-analysis of the microbiome in composition, diversity, and ecological function from 13 marine sponges further supported that bacterial communities associated with HMA and LMA sponges have evolved two distinct environmental adaptation strategies. We propose that the different adaptive ways of sponges responding to the environment may be responsible for their successful evolution and their competence in global ocean change.
IMPORTANCE: During long-term evolution, sponge holobionts, among the oldest symbiotic relationships between microbes and metazoans, developed two distinct phenotypes with high- and low-microbial abundance (HMA and LMA). Despite sporadic studies indicating that the characteristic microbial assemblages present in HMA and LMA sponges, the adaptation strategies of symbionts responding to environments are still unclear. This deficiency limits our understanding of the selection of symbionts and the ecological functions during the evolutionary history and the adaptative assessment of HMA and LMA sponges in variable environments. Here, we explored symbiotic communities with two distinct phenotypes in a 1-year dynamic environment and combined with the meta-analysis of 13 sponges. The different strategies of symbionts in adapting to the environment were basically drawn: microbes with LMA were more acclimated to environmental changes, forming relatively loose-connected communities, while HMA developed relatively tight-connected and more similar communities beyond the divergence of species and geographical location.},
}
RevDate: 2024-10-15
Constructed wetlands combined with microbial fuel cells (CW-MFCs) as a sustainable technology for leachate treatment and power generation.
RSC advances, 14(44):32073-32100.
The physical and chemical treatment processes of leachate are not only costly but can also possibly produce harmful by products. Constructed wetlands (CW) has been considered a promising alternative technology for leachate treatment due to less demand for energy, economic, ecological benefits, and simplicity of operations. Various trends and approaches for the application of CW for leachate treatment have been discussed in this review along with offering an informatics peek of the recent innovative developments in CW technology and its perspectives. In addition, coupling CW with microbial fuel cells (MFCs) has proven to produce renewable energy (electricity) while treating contaminants in leachate wastewaters (CW-MFC). The combination of CW-MFC is a promising bio electrochemical that plays symbiotic among plant microorganisms in the rhizosphere of an aquatic plant that convert sun electricity is transformed into bioelectricity with the aid of using the formation of radical secretions, as endogenous substrates, and microbial activity. Several researchers study and try to find out the application of CW-MFC for leachate treatment, along with this system and performance. Several key elements for the advancement of CW-MFC technology such as bioelectricity, reactor configurations, plant species, and electrode materials, has been comprehensively discussed and future research directions were suggested for further improving the performance. Overall, CW-MFC may offer an eco-friendly approach to protecting the aquatic environment and come with built-in advantages for visual appeal and animal habitats using natural materials such as gravel, soil, electroactive bacteria, and plants under controlled condition.
Additional Links: PMID-39399250
PubMed:
Citation:
show bibtex listing
hide bibtex listing
@article {pmid39399250,
year = {2024},
author = {Arliyani, I and Noori, MT and Ammarullah, MI and Tangahu, BV and Mangkoedihardjo, S and Min, B},
title = {Constructed wetlands combined with microbial fuel cells (CW-MFCs) as a sustainable technology for leachate treatment and power generation.},
journal = {RSC advances},
volume = {14},
number = {44},
pages = {32073-32100},
pmid = {39399250},
issn = {2046-2069},
abstract = {The physical and chemical treatment processes of leachate are not only costly but can also possibly produce harmful by products. Constructed wetlands (CW) has been considered a promising alternative technology for leachate treatment due to less demand for energy, economic, ecological benefits, and simplicity of operations. Various trends and approaches for the application of CW for leachate treatment have been discussed in this review along with offering an informatics peek of the recent innovative developments in CW technology and its perspectives. In addition, coupling CW with microbial fuel cells (MFCs) has proven to produce renewable energy (electricity) while treating contaminants in leachate wastewaters (CW-MFC). The combination of CW-MFC is a promising bio electrochemical that plays symbiotic among plant microorganisms in the rhizosphere of an aquatic plant that convert sun electricity is transformed into bioelectricity with the aid of using the formation of radical secretions, as endogenous substrates, and microbial activity. Several researchers study and try to find out the application of CW-MFC for leachate treatment, along with this system and performance. Several key elements for the advancement of CW-MFC technology such as bioelectricity, reactor configurations, plant species, and electrode materials, has been comprehensively discussed and future research directions were suggested for further improving the performance. Overall, CW-MFC may offer an eco-friendly approach to protecting the aquatic environment and come with built-in advantages for visual appeal and animal habitats using natural materials such as gravel, soil, electroactive bacteria, and plants under controlled condition.},
}
RevDate: 2024-10-15
Beyond Boundaries: The Role of Artificial Intelligence in Shaping the Future Careers of Medical Students in Saudi Arabia.
Cureus, 16(9):e69332.
INTRODUCTION: Artificial intelligence (AI) stands at the forefront of revolutionizing healthcare, wielding its computational prowess to navigate the labyrinth of medical data with unprecedented precision. In this study, we delved into the perspectives of medical students in the Kingdom of Saudi Arabia (KSA) regarding AI's seismic impact on their careers and the medical landscape.
METHODS: A cross-sectional study conducted from February to December 2023 examined the impact of AI on the future of medical students' careers in KSA, surveying approximately 400 participants, including Saudi medical students and interns, and uncovering a fascinating tapestry of perceptions.
RESULTS: Astonishingly, 75.4% of respondents boasted familiarity with AI, heralding its transformative potential. A resounding 88.9% lauded its capacity to enrich medical education, marking a paradigm shift in learning approaches. However, amidst this wave of optimism, shadows of apprehension loomed. A staggering 42.5% harbored concerns of AI precipitating job displacement, while 34.4% envisioned a future where AI usurps traditional doctor roles. Despite this dichotomy, there existed a unanimous recognition of the symbiotic relationship between AI and human healthcare professionals, heralding an era of collaborative synergy.
CONCLUSION: Our findings underscored a critical need for educational initiatives to assuage fears and facilitate the seamless integration of AI into clinical practice. Moreover, AI's burgeoning influence in diagnostic radiology and personalized healthcare plans emerged as catalysts propelling the domain of precision medicine into uncharted realms of innovation. As AI reshapes the contours of healthcare delivery, it not only promises unparalleled efficiency but also holds the key to unlocking new frontiers in treatment outcomes and accessibility, heralding a transformative epoch in the annals of medicine.
Additional Links: PMID-39398766
PubMed:
Citation:
show bibtex listing
hide bibtex listing
@article {pmid39398766,
year = {2024},
author = {Alammari, DM and Melebari, RE and Alshaikh, JA and Alotaibi, LB and Basabeen, HS and Saleh, AF},
title = {Beyond Boundaries: The Role of Artificial Intelligence in Shaping the Future Careers of Medical Students in Saudi Arabia.},
journal = {Cureus},
volume = {16},
number = {9},
pages = {e69332},
pmid = {39398766},
issn = {2168-8184},
abstract = {INTRODUCTION: Artificial intelligence (AI) stands at the forefront of revolutionizing healthcare, wielding its computational prowess to navigate the labyrinth of medical data with unprecedented precision. In this study, we delved into the perspectives of medical students in the Kingdom of Saudi Arabia (KSA) regarding AI's seismic impact on their careers and the medical landscape.
METHODS: A cross-sectional study conducted from February to December 2023 examined the impact of AI on the future of medical students' careers in KSA, surveying approximately 400 participants, including Saudi medical students and interns, and uncovering a fascinating tapestry of perceptions.
RESULTS: Astonishingly, 75.4% of respondents boasted familiarity with AI, heralding its transformative potential. A resounding 88.9% lauded its capacity to enrich medical education, marking a paradigm shift in learning approaches. However, amidst this wave of optimism, shadows of apprehension loomed. A staggering 42.5% harbored concerns of AI precipitating job displacement, while 34.4% envisioned a future where AI usurps traditional doctor roles. Despite this dichotomy, there existed a unanimous recognition of the symbiotic relationship between AI and human healthcare professionals, heralding an era of collaborative synergy.
CONCLUSION: Our findings underscored a critical need for educational initiatives to assuage fears and facilitate the seamless integration of AI into clinical practice. Moreover, AI's burgeoning influence in diagnostic radiology and personalized healthcare plans emerged as catalysts propelling the domain of precision medicine into uncharted realms of innovation. As AI reshapes the contours of healthcare delivery, it not only promises unparalleled efficiency but also holds the key to unlocking new frontiers in treatment outcomes and accessibility, heralding a transformative epoch in the annals of medicine.},
}
RevDate: 2024-10-15
Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana.
Journal of pesticide science, 49(3):186-194.
Strigolactones (SLs) are a class of plant hormones that play several roles in plants, such as suppressing shoot branching and promoting arbuscular mycorrhizal symbiosis. The positive regulation of plant disease resistance by SLs has recently been demonstrated by analyses using SL-related mutants. In Arabidopsis, SL-mediated signaling has been reported to modulate salicylic acid-mediated disease resistance, in which the priming of plant immunity plays an important role. In this study, we analyzed the effect of the synthetic SL analogue rac-GR24 on resistance against necrotrophic pathogen Botrytis cinerea. In rac-GR24-treated plants, disease resistance against B. cinerea was enhanced in an ethylene- and camalexin-dependent manners. Expression of the ethylene-related genes and the camalexin biosynthetic gene and camalexin accumulation after pathogen infection were enhanced by immune priming in rac-GR24-treated plants. These suggest that SL-mediated immune priming is effective for many types of resistance mechanisms in plant self-defense systems.
Additional Links: PMID-39398504
PubMed:
Citation:
show bibtex listing
hide bibtex listing
@article {pmid39398504,
year = {2024},
author = {Fujita, M and Tanaka, T and Kusajima, M and Inoshima, K and Narita, F and Nakamura, H and Asami, T and Maruyama-Nakashita, A and Nakashita, H},
title = {Enhanced disease resistance against Botrytis cinerea by strigolactone-mediated immune priming in Arabidopsis thaliana.},
journal = {Journal of pesticide science},
volume = {49},
number = {3},
pages = {186-194},
pmid = {39398504},
issn = {1348-589X},
abstract = {Strigolactones (SLs) are a class of plant hormones that play several roles in plants, such as suppressing shoot branching and promoting arbuscular mycorrhizal symbiosis. The positive regulation of plant disease resistance by SLs has recently been demonstrated by analyses using SL-related mutants. In Arabidopsis, SL-mediated signaling has been reported to modulate salicylic acid-mediated disease resistance, in which the priming of plant immunity plays an important role. In this study, we analyzed the effect of the synthetic SL analogue rac-GR24 on resistance against necrotrophic pathogen Botrytis cinerea. In rac-GR24-treated plants, disease resistance against B. cinerea was enhanced in an ethylene- and camalexin-dependent manners. Expression of the ethylene-related genes and the camalexin biosynthetic gene and camalexin accumulation after pathogen infection were enhanced by immune priming in rac-GR24-treated plants. These suggest that SL-mediated immune priming is effective for many types of resistance mechanisms in plant self-defense systems.},
}
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ESP Quick Facts
ESP Origins
In the early 1990's, Robert Robbins was a faculty member at Johns Hopkins, where he directed the informatics core of GDB — the human gene-mapping database of the international human genome project. To share papers with colleagues around the world, he set up a small paper-sharing section on his personal web page. This small project evolved into The Electronic Scholarly Publishing Project.
ESP Support
In 1995, Robbins became the VP/IT of the Fred Hutchinson Cancer Research Center in Seattle, WA. Soon after arriving in Seattle, Robbins secured funding, through the ELSI component of the US Human Genome Project, to create the original ESP.ORG web site, with the formal goal of providing free, world-wide access to the literature of classical genetics.
ESP Rationale
Although the methods of molecular biology can seem almost magical to the uninitiated, the original techniques of classical genetics are readily appreciated by one and all: cross individuals that differ in some inherited trait, collect all of the progeny, score their attributes, and propose mechanisms to explain the patterns of inheritance observed.
ESP Goal
In reading the early works of classical genetics, one is drawn, almost inexorably, into ever more complex models, until molecular explanations begin to seem both necessary and natural. At that point, the tools for understanding genome research are at hand. Assisting readers reach this point was the original goal of The Electronic Scholarly Publishing Project.
ESP Usage
Usage of the site grew rapidly and has remained high. Faculty began to use the site for their assigned readings. Other on-line publishers, ranging from The New York Times to Nature referenced ESP materials in their own publications. Nobel laureates (e.g., Joshua Lederberg) regularly used the site and even wrote to suggest changes and improvements.
ESP Content
When the site began, no journals were making their early content available in digital format. As a result, ESP was obliged to digitize classic literature before it could be made available. For many important papers — such as Mendel's original paper or the first genetic map — ESP had to produce entirely new typeset versions of the works, if they were to be available in a high-quality format.
ESP Help
Early support from the DOE component of the Human Genome Project was critically important for getting the ESP project on a firm foundation. Since that funding ended (nearly 20 years ago), the project has been operated as a purely volunteer effort. Anyone wishing to assist in these efforts should send an email to Robbins.
ESP Plans
With the development of methods for adding typeset side notes to PDF files, the ESP project now plans to add annotated versions of some classical papers to its holdings. We also plan to add new reference and pedagogical material. We have already started providing regularly updated, comprehensive bibliographies to the ESP.ORG site.
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Dinosaur tail, complete with feathers, found preserved in amber.
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Mysterious fast radio burst (FRB) detected in the distant universe.
Big Data & Informatics
Big Data: Buzzword or Big Deal?
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