Painting by Artemesia Gentileschi: Judith Beheading Holofernes shows the scene of Judith beheading Holofernes, common in art since the early Renaissance. The subject represents an episode (from the apocryphal Book of Judith in the Old Testament) which recounts the assassination of the Assyrian general Holofernes by the Israelite heroine Judith. The painting shows the moment when Judith, helped by her maidservant, beheads the general after he has fallen asleep drunk. The painting is relentlessly physical, from the wide spurts of blood to the energy of the two women as they perform the act. Although the painting depicts a classic scene from the Bible, Gentileschi drew herself as Judith and her mentor Agostino Tassi, who was tried in court for her rape, as Holofernes. Gentileschi's biographer Mary Garrard famously proposed an autobiographical reading of the painting, stating that it functions as "a cathartic expression of the artist's private, and perhaps repressed, rage."

The Mayflower arrives at Cape Cod bearing Brownist Pilgrims.

English philosopher Francis Bacon publishes Novum Organum (New Instrument), which seeks to add inductive reasoning to the tools of deductive inquiry that had been outlined in Aristotle's treatises of logic known as the Organum.

(no entry for this year)

Jamestown massacre: Algonquian natives kill 347 English settlers outside Jamestown, Virginia (one-third of the colony's population) and burn the Henricus settlement.

The third English dictionary, English Dictionarie, and the first to be called a dictionary is published by Henry Cockeram, listing difficult words with definitions.

Gaspard Bauhin publishes Pinax Theatri Botanici describing some 6,000 plants.

Painting by Frans Hals: The Laughing Cavalier has been described as "one of the most brilliant of all Baroque portraits". The title is an invention of the Victorian public and press, dating from its exhibition in the opening display at the Bethnal Green Museum in 1872–75, just after its arrival in England, after which it was regularly reproduced as a print, and became among of the best known old master paintings in Britain. The unknown subject is in fact not laughing, but can be said to have an enigmatic smile, much amplified by his upturned moustache.

Galileo presents to Cesi, founder of the Lincean Academy, a "little eyeglass" (a microscope). The invention will enable the Linceans to study natural objects with unprecedented precision. They will start with bees, then move on to flies and dust mites.

English settlers occupy the islands of Barbados and St. Kitts.

Pieter Jansz Schaghen writes to the States General of the Dutch Republic announcing the purchase of Manhattan from the Native Americans.

The Dutch West India Company imports 11 black male slaves into the New Netherlands.

Aurochs go extinct.

Painting by Nicolas Poussin: The Martyrdom of St. Erasmus

William Harvey publishes Exercitatio Anatomica de Motu Cordis et Sanguinis in Animalibus (Latin for "An Anatomical Exercise on the Motion of the Heart and Blood in Living Beings"). The book established the circulation of the blood and was a landmark in the history of physiology. Just as important as its substance was its method. Harvey combined observations, experiments, measurements, and hypotheses in extraordinary fashion to arrive at his doctrine. His work is a model of its kind. It had an immediate and far-reaching influence on Harvey's contemporaries. In the book, Harvey investigated the effect of ligatures on blood flow. The book also argued that blood was pumped around the body in a "double circulation", where after being returned to the heart, it is recirculated in a closed system to the lungs and back to the heart, where it is returned to the main circulation.

English troops capture Québec.